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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

2272R-2

Symbol

slpr

Full Name

slipper

CG No

CG2272

Old CG No

CG2272

Synonyms

dMLK, MLK2, Mlk2, dMLK2, CG2272, slpr, Mixed lineage protein kinase 2, slipper, Slpr

Accession No (Link to NCBI)

NM_132230.2

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

male lethal

Map Viewer

Reference
[Please submit your publication]

Lesch C, Jo J, Wu Y, Fish GS, Galko MJ.<br> A targeted UAS-RNAi screen in Drosophila larvae identifies wound closure genes regulating distinct cellular processes.<br> Genetics (2010) 186(3) 943-57 [ PubMed ID = 20813879 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCTGTGGACCGCGCTGTATGAC

Primer Seq. 3'

aatctagaggtaccTTGAGACTTCCTCCCCTGGCA

Predicted Fragment Size

IR fragment full Seq

0001 CTGTGGACCG CGCTGTATGA CTACGATGCC CAGGGCGAGG ATGAGCTGAC ACTGCGACGC 
0061 GGCGAAATCG TTGTGGTCCT GTCCACGGAC AGCGAGGTGT CTGGCGATGT GGGCTGGTGG 
0121 ACCGGCAAGA TTGGAGATAA GGTCGGTGTC TTTCCCAAGG ACTTTGTCAC CGATGAGGAC 
0181 CCACTGCAAC TGAATGTCTC GTCCGCCATT GGCGACNATC CAGCCGCATG AGATCGAATA 
0241 CAATGAGCTG GACATCAAGG AAGTTATCGG CTCCGGTGGC TTCTGCAAGG TCCATCGTGG 
0301 CTACTACGAT GGCGANGGAG GTGGCTATTA AGATTGCCCA TCAAACGGGC GAGGATGATA 
0361 TGCAGAGGAT GCNGGGACAA TGTCCTGCAG GAGNGCCAAA CTGTTTTNGG GCCCTCAAGC 
0421 ACGAAAATAT AGCAGCCCTG CGAGGAGTAT GTCTGAATAC CAAACTCTGT CTGGTGATGG 
0481 AGTATGCCAG GGGAGGAAGT CTCAA

in silico PCR Fragment

0001 CTGTGGACCG CGCTGTATGA CTACGATGCC CAGGGCGAGG ATGAGCTGAC ACTGCGACGC 
0061 GGCGAAATCG TTGTGGTCCT GTCCACGGAC AGCGAGGTGT CTGGCGATGT GGGCTGGTGG 
0121 ACCGGCAAGA TTGGAGATAA GGTCGGTGTC TTTCCCAAGG ACTTTGTCAC CGATGAGGAC 
0181 CCACTGCAAC TGAATGTCTC GTCCGCCATT GGCGAC-ATC CAGCCGCATG AGATCGAATA 
0241 CAATGAGCTG GACATCAAGG AAGTTATCGG CTCCGGTGGC TTCTGCAAGG TCCATCGTGG 
0301 CTACTACGAT GGCGA-GGAG GTGGCTATTA AGATTGCCCA TCAAACGGGC GAGGATGATA 
0361 TGCAGAGGAT GC-GGGACAA TGTCCTGCAG GAG-GCCAAA CTGTTTT-GG GCCCTCAAGC 
0421 ACGAAAATAT AGCAGCCCTG CGAGGAGTAT GTCTGAATAC CAAACTCTGT CTGGTGATGG 
0481 AGTATGCCAG GGGAGGAAGT CTCAA

Assemble Data

<PRE>

2272R-2.IR_full       1   CTGTGGACCGCGCTGTATGACTACGATGCCCAGGGCGAGGATGAGCTGACACTGCGACGC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2272R-2.in silico     1   CTGTGGACCGCGCTGTATGACTACGATGCCCAGGGCGAGGATGAGCTGACACTGCGACGC 60


2272R-2.IR_full       61  GGCGAAATCGTTGTGGTCCTGTCCACGGACAGCGAGGTGTCTGGCGATGTGGGCTGGTGG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2272R-2.in silico     61  GGCGAAATCGTTGTGGTCCTGTCCACGGACAGCGAGGTGTCTGGCGATGTGGGCTGGTGG 120


2272R-2.IR_full       121 ACCGGCAAGATTGGAGATAAGGTCGGTGTCTTTCCCAAGGACTTTGTCACCGATGAGGAC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2272R-2.in silico     121 ACCGGCAAGATTGGAGATAAGGTCGGTGTCTTTCCCAAGGACTTTGTCACCGATGAGGAC 180


2272R-2.IR_full       181 CCACTGCAACTGAATGTCTCGTCCGCCATTGGCGAC<span class="snp"><tt>N</tt></span>ATCCAGCCGCATGAGATCGAATA 240
                          ||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||
2272R-2.in silico     181 CCACTGCAACTGAATGTCTCGTCCGCCATTGGCGAC<span class="snp"><tt>-</tt></span>ATCCAGCCGCATGAGATCGAATA 240


2272R-2.IR_full       241 CAATGAGCTGGACATCAAGGAAGTTATCGGCTCCGGTGGCTTCTGCAAGGTCCATCGTGG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2272R-2.in silico     241 CAATGAGCTGGACATCAAGGAAGTTATCGGCTCCGGTGGCTTCTGCAAGGTCCATCGTGG 300


2272R-2.IR_full       301 CTACTACGATGGCGA<span class="snp"><tt>N</tt></span>GGAGGTGGCTATTAAGATTGCCCATCAAACGGGCGAGGATGATA 360
                          |||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||
2272R-2.in silico     301 CTACTACGATGGCGA<span class="snp"><tt>-</tt></span>GGAGGTGGCTATTAAGATTGCCCATCAAACGGGCGAGGATGATA 360


2272R-2.IR_full       361 TGCAGAGGATGC<span class="snp"><tt>N</tt></span>GGGACAATGTCCTGCAGGAG<span class="snp"><tt>N</tt></span>GCCAAACTGTTTT<span class="snp"><tt>N</tt></span>GGGCCCTCAAGC 420
                          ||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||
2272R-2.in silico     361 TGCAGAGGATGC<span class="snp"><tt>-</tt></span>GGGACAATGTCCTGCAGGAG<span class="snp"><tt>-</tt></span>GCCAAACTGTTTT<span class="snp"><tt>-</tt></span>GGGCCCTCAAGC 420


2272R-2.IR_full       421 ACGAAAATATAGCAGCCCTGCGAGGAGTATGTCTGAATACCAAACTCTGTCTGGTGATGG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2272R-2.in silico     421 ACGAAAATATAGCAGCCCTGCGAGGAGTATGTCTGAATACCAAACTCTGTCTGGTGATGG 480


2272R-2.IR_full       481 AGTATGCCAGGGGAGGAAGTCTCAA 505
                          |||||||||||||||||||||||||
2272R-2.in silico     481 AGTATGCCAGGGGAGGAAGTCTCAA 505

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	1	NM_132230.2	CG2272-RA (slpr), mRNA
0.2	1	2	2	6	NM_143605.2	CG11339-RA (CG11339), mRNA
0	0	3	2	3	NM_140866.1	CG9299-RA (CG9299), mRNA
0	0	2	4	4	NM_132019.1	CG4096-RA (CG4096), mRNA
0	0	2	3	2	NM_206251.2	CG16973-RD, transcript variant D (msn), mRNA
0	0	2	3	2	NM_206249.2	CG16973-RE, transcript variant E (msn), mRNA
0	0	2	3	2	NM_206252.2	CG16973-RB, transcript variant B (msn), mRNA
0	0	2	3	2	NM_079940.4	CG16973-RA, transcript variant A (msn), mRNA
0	0	2	3	2	NM_206250.2	CG16973-RC, transcript variant C (msn), mRNA
0	0	2	2	2	NM_137846.2	CG17807-RA (CG17807), mRNA
0	0	1	5	4	NM_138031.1	CG3173-RA (CG3173), mRNA
0	0	1	4	6	NM_164554.2	CG2822-RB, transcript variant B (Shaw), mRNA
0	0	1	4	5	NM_057373.2	CG2822-RA, transcript variant A (Shaw), mRNA
0	0	1	4	2	NM_130577.2	CG3095-RA (hfw), mRNA
0	0	1	2	5	NM_057425.2	CG9450-RA (tud), mRNA
0	0	1	2	3	NM_080148.2	CG2522-RA (Gtp-bp), mRNA
0	0	1	2	2	NM_137339.2	CG9646-RA (CG9646), mRNA
0	0	0	6	10	NM_140033.1	CG4684-RA (nwk), mRNA
0	0	0	6	8	NM_079863.2	CG2048-RC, transcript variant C (dco), mRNA
0	0	0	6	8	NM_170535.1	CG2048-RA, transcript variant A (dco), mRNA
0	0	0	6	8	NM_170536.2	CG2048-RB, transcript variant B (dco), mRNA
0	0	0	6	4	NM_079902.2	CG6741-RB, transcript variant B (a), mRNA
0	0	0	6	4	NM_166514.1	CG6741-RA, transcript variant A (a), mRNA
0	0	0	6	4	NM_001014543.1	CG6741-RC, transcript variant C (a), mRNA
0	0	0	6	2	NM_143759.2	CG6760-RA (l(3)70Da), mRNA
0	0	0	5	13	NM_001014545.1	CG33519-RB (Unc-89), mRNA
0	0	0	5	7	NM_141809.1	CG6715-RA (KP78a), mRNA
0	0	0	5	4	NM_132051.1	CG3726-RA (CG3726), mRNA
0	0	0	5	2	NM_078500.3	CG4317-RA (Mipp2), mRNA
0	0	0	4	6	NM_138264.1	CG9173-RA (CG9173), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

dme04013 : MAPK signaling pathway - fly

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