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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
2272R-1 |
| Symbol |
slpr |
Full Name |
slipper |
| CG No |
CG2272 |
Old CG No |
CG2272 |
| Synonyms |
dMLK, MLK2, Mlk2, dMLK2, CG2272, slpr, Mixed lineage protein kinase 2, slipper, Slpr |
| Accession No (Link to NCBI) |
NM_132230.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Fairchild MJ, Islam F, Tanentzapf G.<br> Identification of genetic networks that act in the somatic cells of the testis to mediate the developmental program of spermatogenesis.<br> PLoS Genet (2017) 13(9) e1007026
[
PubMed ID = 28957323
]
[
RRC reference
]
Swarup S, Pradhan-Sundd T, Verheyen EM.<br> Genome-wide identification of phospho-regulators of Wnt signaling in Drosophila.<br> Development (2015) 142(8) 1502-15
[
PubMed ID = 25852200
]
[
RRC reference
]
Neisch AL, Speck O, Stronach B, Fehon RG.<br> Rho1 regulates apoptosis via activation of the JNK signaling pathway at the plasma membrane.<br> J Cell Biol (2010) 189(2) 311-23
[
PubMed ID = 20404112
]
[
RRC reference
]
Lesch C, Jo J, Wu Y, Fish GS, Galko MJ.<br> A targeted UAS-RNAi screen in Drosophila larvae identifies wound closure genes regulating distinct cellular processes.<br> Genetics (2010) 186(3) 943-57
[
PubMed ID = 20813879
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCTGTGGACCGCGCTGTATGAC |
| Primer Seq. 3' |
aatctagaggtaccTTGAGACTTCCTCCCCTGGCA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CTGTGGACCG CGCTGTATGA CTACGATGCC CAGGGCGAGG ATGAGCTGAC ACTGCGACGC
0061 GGCGAAATCG TTGTGGTCCT GTCCACGGAC AGCGAGGTGT CTGGCGATGT GGGCTGGTGG
0121 ACCGGCAAGA TTGGAGATAA GGTCGGTGTC TTTCCCAAGG ACTTTGTCAC CGATGAGGAC
0181 CCACTGCAAC TGAATGTCTC GTCCGCCATT GGCGACNATC CAGCCGCATG AGATCGAATA
0241 CAATGAGCTG GACATCAAGG AAGTTATCGG CTCCGGTGGC TTCTGCAAGG TCCATCGTGG
0301 CTACTACGAT GGCGANGGAG GTGGCTATTA AGATTGCCCA TCAAACGGGC GAGGATGATA
0361 TGCAGAGGAT GCNGGGACAA TGTCCTGCAG GAGNGCCAAA CTGTTTTNGG GCCCTCAAGC
0421 ACGAAAATAT AGCAGCCCTG CGAGGAGTAT GTCTGAATAC CAAACTCTGT CTGGTGATGG
0481 AGTATGCCAG GGGAGGAAGT CTCAA |
| in silico PCR Fragment |
0001 CTGTGGACCG CGCTGTATGA CTACGATGCC CAGGGCGAGG ATGAGCTGAC ACTGCGACGC
0061 GGCGAAATCG TTGTGGTCCT GTCCACGGAC AGCGAGGTGT CTGGCGATGT GGGCTGGTGG
0121 ACCGGCAAGA TTGGAGATAA GGTCGGTGTC TTTCCCAAGG ACTTTGTCAC CGATGAGGAC
0181 CCACTGCAAC TGAATGTCTC GTCCGCCATT GGCGAC-ATC CAGCCGCATG AGATCGAATA
0241 CAATGAGCTG GACATCAAGG AAGTTATCGG CTCCGGTGGC TTCTGCAAGG TCCATCGTGG
0301 CTACTACGAT GGCGA-GGAG GTGGCTATTA AGATTGCCCA TCAAACGGGC GAGGATGATA
0361 TGCAGAGGAT GC-GGGACAA TGTCCTGCAG GAG-GCCAAA CTGTTTT-GG GCCCTCAAGC
0421 ACGAAAATAT AGCAGCCCTG CGAGGAGTAT GTCTGAATAC CAAACTCTGT CTGGTGATGG
0481 AGTATGCCAG GGGAGGAAGT CTCAA |
| Assemble Data |
<PRE>
2272R-1.IR_full 1 CTGTGGACCGCGCTGTATGACTACGATGCCCAGGGCGAGGATGAGCTGACACTGCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2272R-1.in silico 1 CTGTGGACCGCGCTGTATGACTACGATGCCCAGGGCGAGGATGAGCTGACACTGCGACGC 60
2272R-1.IR_full 61 GGCGAAATCGTTGTGGTCCTGTCCACGGACAGCGAGGTGTCTGGCGATGTGGGCTGGTGG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2272R-1.in silico 61 GGCGAAATCGTTGTGGTCCTGTCCACGGACAGCGAGGTGTCTGGCGATGTGGGCTGGTGG 120
2272R-1.IR_full 121 ACCGGCAAGATTGGAGATAAGGTCGGTGTCTTTCCCAAGGACTTTGTCACCGATGAGGAC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2272R-1.in silico 121 ACCGGCAAGATTGGAGATAAGGTCGGTGTCTTTCCCAAGGACTTTGTCACCGATGAGGAC 180
2272R-1.IR_full 181 CCACTGCAACTGAATGTCTCGTCCGCCATTGGCGAC<span class="snp"><tt>N</tt></span>ATCCAGCCGCATGAGATCGAATA 240
||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||
2272R-1.in silico 181 CCACTGCAACTGAATGTCTCGTCCGCCATTGGCGAC<span class="snp"><tt>-</tt></span>ATCCAGCCGCATGAGATCGAATA 240
2272R-1.IR_full 241 CAATGAGCTGGACATCAAGGAAGTTATCGGCTCCGGTGGCTTCTGCAAGGTCCATCGTGG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2272R-1.in silico 241 CAATGAGCTGGACATCAAGGAAGTTATCGGCTCCGGTGGCTTCTGCAAGGTCCATCGTGG 300
2272R-1.IR_full 301 CTACTACGATGGCGA<span class="snp"><tt>N</tt></span>GGAGGTGGCTATTAAGATTGCCCATCAAACGGGCGAGGATGATA 360
|||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||||
2272R-1.in silico 301 CTACTACGATGGCGA<span class="snp"><tt>-</tt></span>GGAGGTGGCTATTAAGATTGCCCATCAAACGGGCGAGGATGATA 360
2272R-1.IR_full 361 TGCAGAGGATGC<span class="snp"><tt>N</tt></span>GGGACAATGTCCTGCAGGAG<span class="snp"><tt>N</tt></span>GCCAAACTGTTTT<span class="snp"><tt>N</tt></span>GGGCCCTCAAGC 420
||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||<span class="snp"><tt> </tt></span>||||||||||||
2272R-1.in silico 361 TGCAGAGGATGC<span class="snp"><tt>-</tt></span>GGGACAATGTCCTGCAGGAG<span class="snp"><tt>-</tt></span>GCCAAACTGTTTT<span class="snp"><tt>-</tt></span>GGGCCCTCAAGC 420
2272R-1.IR_full 421 ACGAAAATATAGCAGCCCTGCGAGGAGTATGTCTGAATACCAAACTCTGTCTGGTGATGG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2272R-1.in silico 421 ACGAAAATATAGCAGCCCTGCGAGGAGTATGTCTGAATACCAAACTCTGTCTGGTGATGG 480
2272R-1.IR_full 481 AGTATGCCAGGGGAGGAAGTCTCAA 505
|||||||||||||||||||||||||
2272R-1.in silico 481 AGTATGCCAGGGGAGGAAGTCTCAA 505
</PRE>
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
1 |
NM_132230.2 |
CG2272-RA (slpr), mRNA |
| 0.2 |
1 |
2 |
2 |
6 |
NM_143605.2 |
CG11339-RA (CG11339), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_140866.1 |
CG9299-RA (CG9299), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_132019.1 |
CG4096-RA (CG4096), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_206251.2 |
CG16973-RD, transcript variant D (msn), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_206249.2 |
CG16973-RE, transcript variant E (msn), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_206252.2 |
CG16973-RB, transcript variant B (msn), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_079940.4 |
CG16973-RA, transcript variant A (msn), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_206250.2 |
CG16973-RC, transcript variant C (msn), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_137846.2 |
CG17807-RA (CG17807), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_138031.1 |
CG3173-RA (CG3173), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_164554.2 |
CG2822-RB, transcript variant B (Shaw), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_057373.2 |
CG2822-RA, transcript variant A (Shaw), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_130577.2 |
CG3095-RA (hfw), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_057425.2 |
CG9450-RA (tud), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_080148.2 |
CG2522-RA (Gtp-bp), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_137339.2 |
CG9646-RA (CG9646), mRNA |
| 0 |
0 |
0 |
6 |
10 |
NM_140033.1 |
CG4684-RA (nwk), mRNA |
| 0 |
0 |
0 |
6 |
8 |
NM_079863.2 |
CG2048-RC, transcript variant C (dco), mRNA |
| 0 |
0 |
0 |
6 |
8 |
NM_170535.1 |
CG2048-RA, transcript variant A (dco), mRNA |
| 0 |
0 |
0 |
6 |
8 |
NM_170536.2 |
CG2048-RB, transcript variant B (dco), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_079902.2 |
CG6741-RB, transcript variant B (a), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_166514.1 |
CG6741-RA, transcript variant A (a), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_001014543.1 |
CG6741-RC, transcript variant C (a), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_143759.2 |
CG6760-RA (l(3)70Da), mRNA |
| 0 |
0 |
0 |
5 |
13 |
NM_001014545.1 |
CG33519-RB (Unc-89), mRNA |
| 0 |
0 |
0 |
5 |
7 |
NM_141809.1 |
CG6715-RA (KP78a), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_132051.1 |
CG3726-RA (CG3726), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_078500.3 |
CG4317-RA (Mipp2), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_138264.1 |
CG9173-RA (CG9173), mRNA |
|
| Wing Disc |
GFP
 |
Puc
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Caspase
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DAPI
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Merge
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Figure
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|
JNK signal
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Caspase-3 signal
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Pathways (updated: 2024/04/26)
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| KEGG Pathway |
dme04013
:
MAPK signaling pathway - fly
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