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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
2248R-2 |
| Symbol |
Rac1 |
Full Name |
Rac1 |
| CG No |
CG2248 |
Old CG No |
CG2248 |
| Synonyms |
Drac, rac1, DmRAC1, Rac, Drac1, GTPase Rac1, DRac1, Rac GTPase, rac, dRac1, D-Rac 1, Dm Rac1, dRac, DracA, DRac, RacA, D-Rac, D-Rac1, DRacA, Drac1a, Dmrac1, CG2248, Rac1 |
| Accession No (Link to NCBI) |
NM_057602.3 |
| Inserted Chr. |
X |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Baek SH, Kwon YC, Lee H, Choe KM.<br> Rho-family small GTPases are required for cell polarization and directional sensing in Drosophila wound healing.<br> Biochem Biophys Res Commun (2010) 394(3) 488-92
[
PubMed ID = 20184864
]
[
RRC reference
]
Baek SH, Cho HW, Kwon YC, Lee JH, Kim MJ, Lee H, Choe KM.<br> Requirement for Pak3 in Rac1-induced organization of actin and myosin during Drosophila larval wound healing.<br> FEBS Lett (2012) 586(6) 772-7
[
PubMed ID = 22449966
]
[
RRC reference
]
Xavier MJ, Williams MJ.<br> The Rho-family GTPase Rac1 regulates integrin localization in Drosophila immunosurveillance cells.<br> PLoS One (2011) 6(5) e19504
[
PubMed ID = 21603603
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGCAGGCGATCAAGTGCGTCGT |
| Primer Seq. 3' |
aatctagaggtaccCGGTTTTCAGACCCTTCTGCG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GCAGGCGATC AAGTGCGTCG TCGTGGGCGA CGGAGCCGTG GGAAAGACCT GCCTGCTGAT
0061 CAGCTACACG ACCAATGCCT TTCCCGGCGA GTACATACCC ACCGTGTTCG ACAACTACTC
0121 GGCCAACGTG ATGGTGGACN GCCAAGCCCA TCAACCTGGG CCTGTGGGAT ACGGCCGGGC
0181 AGGNAGGACT ACGACCGACT GAGGCCACTG TCTTATCCCC AGACCGATGT CTTCCTCATC
0241 TGCTTCTCGC TGGTGAATCC GGCATCGTTC GAGAACGTGC GGGCCAAGTG GTATCCGGAG
0301 GTGCGCCACC ACTGCCCCAG CACGCCCATC ATCCTGGTGG GCACCAAGCT GGATTTGCGC
0361 GACGACAAGA ACACAATCGA AAAGCTGAGG GACAAGAAAC TGGCGCCCAT CACCTATCCG
0421 CAGGGCCTgG CCATGGCCAA GGAAATCGGA GCGGTCAAGT ATCTGGAGTG CTCGGCCCTG
0481 ACGCAGAAGG GTCTGAAAAC CG |
| in silico PCR Fragment |
0001 GCAGGCGATC AAGTGCGTCG TCGTGGGCGA CGGAGCCGTG GGAAAGACCT GCCTGCTGAT
0061 CAGCTACACG ACCAATGCCT TTCCCGGCGA GTACATACCC ACCGTGTTCG ACAACTACTC
0121 GGCCAACGTG ATGGTGGAC- GCCAAGCCCA TCAACCTGGG CCTGTGGGAT ACGGCCGGGC
0181 AGG-AGGACT ACGACCGACT GAGGCCACTG TCTTATCCCC AGACCGATGT CTTCCTCATC
0241 TGCTTCTCGC TGGTGAATCC GGCATCGTTC GAGAACGTGC GGGCCAAGTG GTATCCGGAG
0301 GTGCGCCACC ACTGCCCCAG CACGCCCATC ATCCTGGTGG GCACCAAGCT GGATTTGCGC
0361 GACGACAAGA ACACAATCGA AAAGCTGAGG GACAAGAAAC TGGCGCCCAT CACCTATCCG
0421 CAGGGCCTGG CCATGGCCAA GGAAATCGGA GCGGTCAAGT ATCTGGAGTG CTCGGCCCTG
0481 ACGCAGAAGG GTCTGAAAAC CG |
| Assemble Data |
<PRE>
2248R-2.IR_full 1 GCAGGCGATCAAGTGCGTCGTCGTGGGCGACGGAGCCGTGGGAAAGACCTGCCTGCTGAT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2248R-2.in silico 1 GCAGGCGATCAAGTGCGTCGTCGTGGGCGACGGAGCCGTGGGAAAGACCTGCCTGCTGAT 60
2248R-2.IR_full 61 CAGCTACACGACCAATGCCTTTCCCGGCGAGTACATACCCACCGTGTTCGACAACTACTC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2248R-2.in silico 61 CAGCTACACGACCAATGCCTTTCCCGGCGAGTACATACCCACCGTGTTCGACAACTACTC 120
2248R-2.IR_full 121 GGCCAACGTGATGGTGGAC<span class="snp"><tt>N</tt></span>GCCAAGCCCATCAACCTGGGCCTGTGGGATACGGCCGGGC 180
|||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||
2248R-2.in silico 121 GGCCAACGTGATGGTGGAC<span class="snp"><tt>-</tt></span>GCCAAGCCCATCAACCTGGGCCTGTGGGATACGGCCGGGC 180
2248R-2.IR_full 181 AGG<span class="snp"><tt>N</tt></span>AGGACTACGACCGACTGAGGCCACTGTCTTATCCCCAGACCGATGTCTTCCTCATC 240
|||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2248R-2.in silico 181 AGG<span class="snp"><tt>-</tt></span>AGGACTACGACCGACTGAGGCCACTGTCTTATCCCCAGACCGATGTCTTCCTCATC 240
2248R-2.IR_full 241 TGCTTCTCGCTGGTGAATCCGGCATCGTTCGAGAACGTGCGGGCCAAGTGGTATCCGGAG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2248R-2.in silico 241 TGCTTCTCGCTGGTGAATCCGGCATCGTTCGAGAACGTGCGGGCCAAGTGGTATCCGGAG 300
2248R-2.IR_full 301 GTGCGCCACCACTGCCCCAGCACGCCCATCATCCTGGTGGGCACCAAGCTGGATTTGCGC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2248R-2.in silico 301 GTGCGCCACCACTGCCCCAGCACGCCCATCATCCTGGTGGGCACCAAGCTGGATTTGCGC 360
2248R-2.IR_full 361 GACGACAAGAACACAATCGAAAAGCTGAGGGACAAGAAACTGGCGCCCATCACCTATCCG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2248R-2.in silico 361 GACGACAAGAACACAATCGAAAAGCTGAGGGACAAGAAACTGGCGCCCATCACCTATCCG 420
2248R-2.IR_full 421 CAGGGCCTGGCCATGGCCAAGGAAATCGGAGCGGTCAAGTATCTGGAGTGCTCGGCCCTG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2248R-2.in silico 421 CAGGGCCTGGCCATGGCCAAGGAAATCGGAGCGGTCAAGTATCTGGAGTGCTCGGCCCTG 480
2248R-2.IR_full 481 ACGCAGAAGGGTCTGAAAACCG 502
||||||||||||||||||||||
2248R-2.in silico 481 ACGCAGAAGGGTCTGAAAACCG 502
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_057602.3 |
CG2248-RA (Rac1), mRNA |
| 3.73 |
18 |
83 |
145 |
121 |
NM_139864.1 |
CG8556-RA (Rac2), mRNA |
| 2.9 |
14 |
7 |
6 |
7 |
NM_166139.2 |
CG8416-RD, transcript variant D (Rho1), mRNA |
| 2.9 |
14 |
7 |
6 |
7 |
NM_206127.1 |
CG8416-RG, transcript variant G (Rho1), mRNA |
| 2.9 |
14 |
7 |
6 |
7 |
NM_206129.1 |
CG8416-RE, transcript variant E (Rho1), mRNA |
| 2.9 |
14 |
7 |
6 |
7 |
NM_134309.1 |
CG8416-RC, transcript variant C (Rho1), mRNA |
| 2.9 |
14 |
7 |
6 |
7 |
NM_057750.3 |
CG8416-RA, transcript variant A (Rho1), mRNA |
| 2.9 |
14 |
7 |
6 |
7 |
NM_206128.1 |
CG8416-RF, transcript variant F (Rho1), mRNA |
| 2.9 |
14 |
7 |
6 |
7 |
NM_134308.2 |
CG8416-RB, transcript variant B (Rho1), mRNA |
| 0.41 |
2 |
8 |
16 |
7 |
NM_001042801.1 |
CG34104-RB, transcript variant B (CG34104), mRNA |
| 0.41 |
2 |
8 |
16 |
3 |
NM_001042802.1 |
CG34104-RA, transcript variant A (CG34104), mRNA |
| 0 |
0 |
21 |
68 |
65 |
NM_167677.1 |
CG12530-RB, transcript variant B (Cdc42), mRNA |
| 0 |
0 |
21 |
68 |
65 |
NM_078690.2 |
CG12530-RA, transcript variant A (Cdc42), mRNA |
| 0 |
0 |
11 |
43 |
34 |
NM_170344.1 |
CG5588-RC, transcript variant C (Mtl), mRNA |
| 0 |
0 |
11 |
43 |
34 |
NM_079809.2 |
CG5588-RB, transcript variant B (Mtl), mRNA |
| 0 |
0 |
11 |
43 |
34 |
NM_170343.1 |
CG5588-RA, transcript variant A (Mtl), mRNA |
| 0 |
0 |
8 |
14 |
33 |
NM_079568.2 |
CG9366-RA (RhoL), mRNA |
| 0 |
0 |
3 |
7 |
5 |
NM_134310.2 |
CG5771-RA, transcript variant A (Rab11), mRNA |
| 0 |
0 |
3 |
7 |
5 |
NM_057822.3 |
CG5771-RB, transcript variant B (Rab11), mRNA |
| 0 |
0 |
3 |
4 |
5 |
NM_134529.2 |
CG9575-RA (Rab35), mRNA |
| 0 |
0 |
1 |
6 |
2 |
NM_134613.2 |
CG1644-RA (Cyp6t1), mRNA |
| 0 |
0 |
1 |
5 |
5 |
NM_080005.2 |
CG3129-RA (Rab-RP4), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_001014545.1 |
CG33519-RB (Unc-89), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_132938.2 |
CG4789-RA (CG4789), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_136468.1 |
CG12826-RA (CG12826), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_170101.1 |
CG5320-RB, transcript variant B (Gdh), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_206550.1 |
CG5320-RE, transcript variant E (Gdh), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_079746.4 |
CG5320-RA, transcript variant A (Gdh), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_206551.1 |
CG5320-RF, transcript variant F (Gdh), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_167736.1 |
CG32521-RC, transcript variant C (CG32521), mRNA |
|
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Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
dme04013
:
MAPK signaling pathway - fly
dme04145
:
Phagosome
dme04148
:
Efferocytosis
dme04310
:
Wnt signaling pathway
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