NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

2199R-1

Symbol

CG2199

Full Name

CG2199

CG No

CG2199

Old CG No

CG2199

Synonyms

CG2199

Accession No (Link to NCBI)

NM_138260.4

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGAGGTGCGATGCGACCATTGC

Primer Seq. 3'

aatctagaggtaccGCGGGCGTCAGCTTAACCGAA

Predicted Fragment Size

IR fragment full Seq

0001 GAGGTGCGAT GCGACCATTG CGGCACAAGC CAAGGGTCCA AGAGTGTTTT TTCAGGTCAG 
0061 AAGGTGTTCC TGGGCAGGAA AATAACAGAC GTACTGGAGA CCATCACCCA CAGAAGCATC 
0121 CCATCATCCT TGCCCATCAA GATATGCTTC GTCTGCACCT CCACATTCAT GTCCAGCGCG 
0181 GCACTAATCG AGAAGGTCCG CGAGACGGTG GACAGGGTAC AGGAGCAGCC CGCCAAGAAG 
0241 ACTAAGGTAG CGGAAATAGA GGAGCCATCG ACACAGGAAA GCGATAAGAA GGCGGTTAAG 
0301 GTGCCAAAAA AGAACACCAC CCTCCGTCAG CGCAGcAAGT CCATAGCAGC TTTCCCGCCA 
0361 AGCTTTGTGA ATGGAGCTAA TACTAATACA GAAATCGAGA TCATTAGTGC TTCGCCCAAG 
0421 AAACTCGATA AGACCCCTAA AAAGCAGATA TCTCGACTCT TCGAGGATAA TCTTAACGAT 
0481 TCGGTTAAGC TGACGCCCGC

in silico PCR Fragment

0001 GAGGTGCGAT GCGACCATTG CGGCACAAGC CAAGGGTCCA AGAGTGTTTT TTCAGGTCAG 
0061 AAGGTGTTCC TGGGCAGGAA AATAACAGAC GTACTGGAGA CCATCACCCA CAGAAGCATC 
0121 CCATCATCCT TGCCCATCAA GATATGCTTC GTCTGCACCT CCACATTCAT GTCCAGCGCG 
0181 GCACTAATCG AGAAGGTCCG CGAGACGGTG GACAGGGTAC AGGAGCAGCC CGCCAAGAAG 
0241 ACTAAGGTAG CGGAAATAGA GGAGCCATCG ACACAGGAAA GCGATAAGAA GGCGGTTAAG 
0301 GTGCCAAAAA AGAACACCAC CCTCCGTCAG CGCAGCAAGT CCATAGCAGC TTTCCCGCCA 
0361 AGCTTTGTGA ATGGAGCTAA TACTAATACA GAAATCGAGA TCATTAGTGC TTCGCCCAAG 
0421 AAACTCGATA AGACCCCTAA AAAGCAGATA TCTCGACTCT TCGAGGATAA TCTTAACGAT 
0481 TCGGTTAAGC TGACGCCCGC

Assemble Data

<PRE>

2199R-1.IR_full       1   GAGGTGCGATGCGACCATTGCGGCACAAGCCAAGGGTCCAAGAGTGTTTTTTCAGGTCAG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2199R-1.in silico     1   GAGGTGCGATGCGACCATTGCGGCACAAGCCAAGGGTCCAAGAGTGTTTTTTCAGGTCAG 60


2199R-1.IR_full       61  AAGGTGTTCCTGGGCAGGAAAATAACAGACGTACTGGAGACCATCACCCACAGAAGCATC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2199R-1.in silico     61  AAGGTGTTCCTGGGCAGGAAAATAACAGACGTACTGGAGACCATCACCCACAGAAGCATC 120


2199R-1.IR_full       121 CCATCATCCTTGCCCATCAAGATATGCTTCGTCTGCACCTCCACATTCATGTCCAGCGCG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2199R-1.in silico     121 CCATCATCCTTGCCCATCAAGATATGCTTCGTCTGCACCTCCACATTCATGTCCAGCGCG 180


2199R-1.IR_full       181 GCACTAATCGAGAAGGTCCGCGAGACGGTGGACAGGGTACAGGAGCAGCCCGCCAAGAAG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2199R-1.in silico     181 GCACTAATCGAGAAGGTCCGCGAGACGGTGGACAGGGTACAGGAGCAGCCCGCCAAGAAG 240


2199R-1.IR_full       241 ACTAAGGTAGCGGAAATAGAGGAGCCATCGACACAGGAAAGCGATAAGAAGGCGGTTAAG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2199R-1.in silico     241 ACTAAGGTAGCGGAAATAGAGGAGCCATCGACACAGGAAAGCGATAAGAAGGCGGTTAAG 300


2199R-1.IR_full       301 GTGCCAAAAAAGAACACCACCCTCCGTCAGCGCAGCAAGTCCATAGCAGCTTTCCCGCCA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2199R-1.in silico     301 GTGCCAAAAAAGAACACCACCCTCCGTCAGCGCAGCAAGTCCATAGCAGCTTTCCCGCCA 360


2199R-1.IR_full       361 AGCTTTGTGAATGGAGCTAATACTAATACAGAAATCGAGATCATTAGTGCTTCGCCCAAG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2199R-1.in silico     361 AGCTTTGTGAATGGAGCTAATACTAATACAGAAATCGAGATCATTAGTGCTTCGCCCAAG 420


2199R-1.IR_full       421 AAACTCGATAAGACCCCTAAAAAGCAGATATCTCGACTCTTCGAGGATAATCTTAACGAT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2199R-1.in silico     421 AAACTCGATAAGACCCCTAAAAAGCAGATATCTCGACTCTTCGAGGATAATCTTAACGAT 480


2199R-1.IR_full       481 TCGGTTAAGCTGACGCCCGC 500
                          ||||||||||||||||||||
2199R-1.in silico     481 TCGGTTAAGCTGACGCCCGC 500

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_167873.1	CG2199-RB, transcript variant B (CG2199), mRNA
100	482	0	0	0	NM_138260.4	CG2199-RA, transcript variant A (CG2199), mRNA
0.41	2	2	3	2	NM_130490.2	CG17778-RA (CG17778), mRNA
0	0	4	4	3	NM_141731.1	CG3996-RA (CG3996), mRNA
0	0	3	2	2	NM_142338.1	CG5175-RA, transcript variant A (kuk), mRNA
0	0	2	6	3	NM_078600.2	CG10521-RA (NetB), mRNA
0	0	2	2	2	NM_137758.2	CG15676-RA (CG15676), mRNA
0	0	1	5	3	NM_167779.1	CG12576-RB, transcript variant B (CG12576), mRNA
0	0	1	5	3	NM_134621.2	CG12576-RA, transcript variant A (CG12576), mRNA
0	0	1	4	5	NM_139686.2	CG10670-RA (Gen), mRNA
0	0	1	3	2	NM_132595.1	CG15725-RA (CG15725), mRNA
0	0	1	3	2	NM_140473.1	CG3349-RA (CG3349), mRNA
0	0	1	2	5	NM_079108.2	CG30170-RA (bgcn), mRNA
0	0	1	2	5	NM_206715.1	CG5541-RA, transcript variant A (CG5541), mRNA
0	0	1	2	5	NM_001042833.1	CG41480-RA (CG41480), mRNA
0	0	1	2	4	NM_167228.1	CG32681-RA (CG32681), mRNA
0	0	1	2	2	NM_206714.1	CG5541-RB, transcript variant B (CG5541), mRNA
0	0	0	9	3	NM_001042810.1	CG10952-RB, transcript variant B (eag), mRNA
0	0	0	9	3	NM_078603.2	CG10952-RA, transcript variant A (eag), mRNA
0	0	0	7	4	NM_135978.1	CG12288-RA (CG12288), mRNA
0	0	0	7	3	NM_169654.2	CG31302-RC, transcript variant C (CG31302), mRNA
0	0	0	6	7	NM_078588.2	CG4303-RA (Bap60), mRNA
0	0	0	5	7	NM_132457.1	CG11122-RA (CG11122), mRNA
0	0	0	5	4	NM_137739.2	CG10795-RA (CG10795), mRNA
0	0	0	5	2	NM_167940.1	CG32305-RA (CG32305), mRNA
0	0	0	4	6	NM_206510.1	CG7131-RB, transcript variant B (CG7131), mRNA
0	0	0	4	6	NM_142449.1	CG7131-RA, transcript variant A (CG7131), mRNA
0	0	0	4	6	NM_206670.1	CG33224-RA (CG33224), mRNA
0	0	0	4	3	NM_135740.1	CG17036-RA (CG17036), mRNA
0	0	0	4	2	NM_132404.1	CG2889-RA (CG2889), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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