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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
2160R-4 |
| Symbol |
Socs44A |
Full Name |
Suppressor of Cytokine Signaling at 44A |
| CG No |
CG2160 |
Old CG No |
CG2160 |
| Synonyms |
dmSocs44A, SOCS, SOCS44A, socs44A, CG2160, Socs44E, Socs44A |
| Accession No (Link to NCBI) |
NM_078935.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
abnormal genitalia |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCACGGCGACCAGAGTCAAAAC |
| Primer Seq. 3' |
aatctagaggtaccTCCTCGCGCTCAAGCCATTTC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CACGGCGAGC CAGAGTCAAA ACCAGAGCAG CCAGCGGANC CAGGCGCTCA CTGGCGGTGG
0061 TGACTCCGCG AGGGACAGGG AGAAGGAAAA GAAGGGCTGG TTCCACTCGC TGACCAGGCG
0121 CAAGAAGTCC GACTCGGCGC TGGCCGTGAC TGCATCCGCC GCCGTCTCCA CCAGTGGATC
0181 GCAGAACAAC AACAACGAAG TGTNGCGTCC TTGAGGCAGC GGACACTTCC ATGGCCAGCT
0241 GCAGCAATGG GGGCACGGGC ACAGGAACTC TGCGCAGAAG GCGGGACAAG GACAAGCGAA
0301 ACGTGTTTGC CAGACTACGA AGGAAGGTGG GCCAAGGGCT CAGCTCCCTG CGAAACTGGC
0361 ACTCCATGGG CGACTGCGAC GACGGCGGAA CTACTGATGC CACCTACGAG TTCCTTACGC
0421 CGGCCATCTG TCCGGAACCC ACGCTACCAT TTACACACGA CTACTTGCGC TTTACCCGGA
0481 AGAAATGGCT TGAGCGCGAG GA |
| in silico PCR Fragment |
0001 CACGGCGA-C CAGAGTCAAA ACCAGAGCAG CCAGCGGAGC CAGGCGCTCA CTGGCGGTGG
0061 TGACTCCCCG AGGGACAGGG AGAAGGAAAA GAAGGGCTGG TTCCACTCGC TGACCAGGCG
0121 CAAGAAGTCC GACTCGGCGC TGGCCGTGAC TGCATCCGCC GCCGTCTCCA CCAGTGGATC
0181 GCAGAACAAC AACAACGAAG TGT-GCGTCC TTGAGGCAGC GGACTCTTCC ATGGCCAGCT
0241 GCAGCAATGG GGGCACGGGC ACAGGAACTC TGCGCAGAAG GCGGGACAAG GACAAGCGAA
0301 ACGTGTTTGC CAGACTACGA AGGAAGGTGG GCCAAGGGCT CAGCTCCCTG CGAAACTGGC
0361 ACTCCATGGG CGACTGCGAC GACGGCGGAA CTACTGATGC CACCTACGAG TTCCTTACGC
0421 CGGCCATCTG TCCGGAACCC ACGCTACCAT TCACACACGA CTACTTGCGC TTTATCCGGA
0481 AGAAATGGCT TGAGCGCGAG GA |
| Assemble Data |
<PRE>
2160R-4.IR_full 1 CACGGCGA<span class="snp"><tt>G</tt></span>CCAGAGTCAAAACCAGAGCAGCCAGCGGA<span class="snp"><tt>N</tt></span>CCAGGCGCTCACTGGCGGTGG 60
||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||
2160R-4.in silico 1 CACGGCGA<span class="snp"><tt>-</tt></span>CCAGAGTCAAAACCAGAGCAGCCAGCGGA<span class="snp"><tt>G</tt></span>CCAGGCGCTCACTGGCGGTGG 60
2160R-4.IR_full 61 TGACTCC<span class="snp"><tt>G</tt></span>CGAGGGACAGGGAGAAGGAAAAGAAGGGCTGGTTCCACTCGCTGACCAGGCG 120
|||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||||
2160R-4.in silico 61 TGACTCC<span class="snp"><tt>C</tt></span>CGAGGGACAGGGAGAAGGAAAAGAAGGGCTGGTTCCACTCGCTGACCAGGCG 120
2160R-4.IR_full 121 CAAGAAGTCCGACTCGGCGCTGGCCGTGACTGCATCCGCCGCCGTCTCCACCAGTGGATC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2160R-4.in silico 121 CAAGAAGTCCGACTCGGCGCTGGCCGTGACTGCATCCGCCGCCGTCTCCACCAGTGGATC 180
2160R-4.IR_full 181 GCAGAACAACAACAACGAAGTGT<span class="snp"><tt>N</tt></span>GCGTCCTTGAGGCAGCGGAC<span class="snp"><tt>A</tt></span>CTTCCATGGCCAGCT 240
|||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||
2160R-4.in silico 181 GCAGAACAACAACAACGAAGTGT<span class="snp"><tt>-</tt></span>GCGTCCTTGAGGCAGCGGAC<span class="snp"><tt>T</tt></span>CTTCCATGGCCAGCT 240
2160R-4.IR_full 241 GCAGCAATGGGGGCACGGGCACAGGAACTCTGCGCAGAAGGCGGGACAAGGACAAGCGAA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2160R-4.in silico 241 GCAGCAATGGGGGCACGGGCACAGGAACTCTGCGCAGAAGGCGGGACAAGGACAAGCGAA 300
2160R-4.IR_full 301 ACGTGTTTGCCAGACTACGAAGGAAGGTGGGCCAAGGGCTCAGCTCCCTGCGAAACTGGC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2160R-4.in silico 301 ACGTGTTTGCCAGACTACGAAGGAAGGTGGGCCAAGGGCTCAGCTCCCTGCGAAACTGGC 360
2160R-4.IR_full 361 ACTCCATGGGCGACTGCGACGACGGCGGAACTACTGATGCCACCTACGAGTTCCTTACGC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2160R-4.in silico 361 ACTCCATGGGCGACTGCGACGACGGCGGAACTACTGATGCCACCTACGAGTTCCTTACGC 420
2160R-4.IR_full 421 CGGCCATCTGTCCGGAACCCACGCTACCATT<span class="snp"><tt>T</tt></span>ACACACGACTACTTGCGCTTTA<span class="snp"><tt>C</tt></span>CCGGA 480
|||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||
2160R-4.in silico 421 CGGCCATCTGTCCGGAACCCACGCTACCATT<span class="snp"><tt>C</tt></span>ACACACGACTACTTGCGCTTTA<span class="snp"><tt>T</tt></span>CCGGA 480
2160R-4.IR_full 481 AGAAATGGCTTGAGCGCGAGGA 502
||||||||||||||||||||||
2160R-4.in silico 481 AGAAATGGCTTGAGCGCGAGGA 502
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_078935.2 |
CG2160-RA (Socs44A), mRNA |
| 0 |
0 |
5 |
2 |
12 |
NM_176557.1 |
CG33106-RB, transcript variant B (mask), mRNA |
| 0 |
0 |
5 |
2 |
12 |
NM_176556.1 |
CG33106-RA, transcript variant A (mask), mRNA |
| 0 |
0 |
3 |
3 |
11 |
NM_132457.1 |
CG11122-RA (CG11122), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_140344.1 |
CG10967-RA (Atg1), mRNA |
| 0 |
0 |
2 |
8 |
13 |
NM_136733.2 |
CG12907-RA (CG12907), mRNA |
| 0 |
0 |
2 |
6 |
2 |
NM_175954.1 |
CG33123-RA (CG33123), mRNA |
| 0 |
0 |
2 |
4 |
5 |
NM_001038984.1 |
CG12877-RC, transcript variant C (CG12877), mRNA |
| 0 |
0 |
2 |
4 |
5 |
NM_143327.2 |
CG12877-RA, transcript variant A (CG12877), mRNA |
| 0 |
0 |
2 |
4 |
5 |
NM_170355.2 |
CG12877-RB, transcript variant B (CG12877), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_141381.2 |
CG15598-RA (Osi17), mRNA |
| 0 |
0 |
2 |
2 |
8 |
NM_169177.1 |
CG2507-RB, transcript variant B (sas), mRNA |
| 0 |
0 |
2 |
2 |
8 |
NM_057263.2 |
CG2507-RA, transcript variant A (sas), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_137937.2 |
CG3502-RA (CG3502), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_080337.2 |
CG6899-RA, transcript variant A (Ptp4E), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_132425.3 |
CG15211-RA (CG15211), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_134494.2 |
CG12235-RA (Arp11), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_164319.2 |
CG1106-RA, transcript variant A (Gel), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_206427.1 |
CG1106-RG, transcript variant G (Gel), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_001043192.1 |
CG1106-RI, transcript variant I (Gel), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_176394.1 |
CG1106-RF, transcript variant F (Gel), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_080126.2 |
CG1106-RB, transcript variant B (Gel), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_206426.1 |
CG1106-RH, transcript variant H (Gel), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_164321.2 |
CG1106-RD, transcript variant D (Gel), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_079939.2 |
CG5580-RA, transcript variant A (sbb), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_206161.1 |
CG5580-RB, transcript variant B (sbb), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_206162.1 |
CG5580-RC, transcript variant C (sbb), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_166094.1 |
CG8174-RC, transcript variant C (SRPK), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_137190.2 |
CG8174-RB, transcript variant B (SRPK), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_166093.1 |
CG8174-RA, transcript variant A (SRPK), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
|
JNK signal
|
Caspase-3 signal
| N |
 |
| ++ |
|
| DB |
 |
| + |
|
| DC |
 |
| ++ |
|
| DM |
 |
| + |
|
| VM |
 |
| + |
|
| VB |
 |
| |
|
|
|
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Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
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|
