NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

2144R-1

Symbol

CG2144

Full Name

CG2144

CG No

CG2144

Old CG No

CG2144

Synonyms

CG2144

Accession No (Link to NCBI)

NM_136448.2

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGGCTGGTCTATACCATCGGCA

Primer Seq. 3'

aatctagaggtaccGGAATCATGGTTCTCGACCTG

Predicted Fragment Size

IR fragment full Seq

0001 GGCTGGTCAT ATACCATCGG CATGCTCACG GCGTTCCTTT GCGGCGCCGG GCTCTTTCTG 
0061 ATTGGCTTCT TTCCGGCCTC CTACTCGGTG GCGGAGAAGG AAAGCACCGT GCCAGAGGGC 
0121 CGGCCCACCG CTCTGCTTGG CATGGAACTG ACGCCGCCGC CACCTGCCTA TGACTCCTTT 
0181 GTGCTGTTAC TGGTCGACGC GTTGCGCGAT GATTTTCCCG ATGCCACGTC TATGCCGGTG 
0241 GCTTATTCTA GGGCCTGCGA GAAGCTTAAG CTCCATGTGG ACATACCGAC GGTGACTATG 
0301 CCACGCTTGA AGAGCATCAC CACCGGCACC CTTTCGAACT TTATCGACAT TGCGCTAAAC 
0361 GTGGGTCATA CGGAGCAGAT GCAGGACTCG TTCTTGCACC GGCTAAAACA GCAGAATCGA 
0421 GTTGTTTCCT TTGCCGGCGA CCACACCTGG GTGAAGCTCT TTCCCAGCGA GTTTACACGG 
0481 CAGGTCGAGA ACCATGATTC C

in silico PCR Fragment

0001 GGCTGGTC-T ATACCATCGG CATGCTCACG GCGTTCCTTT GCGGCGCCGT GCTCTTTCTG 
0061 ATTGGCTTCT TTCCGGCCTC CTACTCGGTG GCGGAGAAGG AAAGCACCGT GCCAGAGGGC 
0121 CGGCCCACCG CTCTGCTTGG CATGGAACTG ACGCCGCCGC CACCTGCCTA TGACTCCTTT 
0181 GTGCTGTTAC TGGTCGACGC GTTGCGCGAT GATTTTCCCG ATGCCACGTC TATGCCGGTG 
0241 GCTTATTCTA GGGCCTGCGA GAAGCTTAAG CTCCATGTGG ACATACCGAC GGTGACTATG 
0301 CCACGCTTGA AGAGCATCAC CACCGGCACC CTTTCGAACT TTATCGACAT TGCGCTAAAC 
0361 GTGGGTCATA CGGAGCAGAT GCAGGACTCG TTCTTGCACC GGCTAAAACA GCAGAATCGA 
0421 GTTGTTTCCT TTGCCGGCGA CCACACCTGG GTGAAGCTCT TTCCCAGCGA GTTTACACGG 
0481 CAGGTCGAGA ACCATGATTC C

Assemble Data

<PRE>

2144R-1.IR_full       1   GGCTGGTC<span class="snp"><tt>A</tt></span>TATACCATCGGCATGCTCACGGCGTTCCTTTGCGGCGCCG<span class="snp"><tt>G</tt></span>GCTCTTTCTG 60
                          ||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||
2144R-1.in silico     1   GGCTGGTC<span class="snp"><tt>-</tt></span>TATACCATCGGCATGCTCACGGCGTTCCTTTGCGGCGCCG<span class="snp"><tt>T</tt></span>GCTCTTTCTG 60


2144R-1.IR_full       61  ATTGGCTTCTTTCCGGCCTCCTACTCGGTGGCGGAGAAGGAAAGCACCGTGCCAGAGGGC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2144R-1.in silico     61  ATTGGCTTCTTTCCGGCCTCCTACTCGGTGGCGGAGAAGGAAAGCACCGTGCCAGAGGGC 120


2144R-1.IR_full       121 CGGCCCACCGCTCTGCTTGGCATGGAACTGACGCCGCCGCCACCTGCCTATGACTCCTTT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2144R-1.in silico     121 CGGCCCACCGCTCTGCTTGGCATGGAACTGACGCCGCCGCCACCTGCCTATGACTCCTTT 180


2144R-1.IR_full       181 GTGCTGTTACTGGTCGACGCGTTGCGCGATGATTTTCCCGATGCCACGTCTATGCCGGTG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2144R-1.in silico     181 GTGCTGTTACTGGTCGACGCGTTGCGCGATGATTTTCCCGATGCCACGTCTATGCCGGTG 240


2144R-1.IR_full       241 GCTTATTCTAGGGCCTGCGAGAAGCTTAAGCTCCATGTGGACATACCGACGGTGACTATG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2144R-1.in silico     241 GCTTATTCTAGGGCCTGCGAGAAGCTTAAGCTCCATGTGGACATACCGACGGTGACTATG 300


2144R-1.IR_full       301 CCACGCTTGAAGAGCATCACCACCGGCACCCTTTCGAACTTTATCGACATTGCGCTAAAC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2144R-1.in silico     301 CCACGCTTGAAGAGCATCACCACCGGCACCCTTTCGAACTTTATCGACATTGCGCTAAAC 360


2144R-1.IR_full       361 GTGGGTCATACGGAGCAGATGCAGGACTCGTTCTTGCACCGGCTAAAACAGCAGAATCGA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2144R-1.in silico     361 GTGGGTCATACGGAGCAGATGCAGGACTCGTTCTTGCACCGGCTAAAACAGCAGAATCGA 420


2144R-1.IR_full       421 GTTGTTTCCTTTGCCGGCGACCACACCTGGGTGAAGCTCTTTCCCAGCGAGTTTACACGG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2144R-1.in silico     421 GTTGTTTCCTTTGCCGGCGACCACACCTGGGTGAAGCTCTTTCCCAGCGAGTTTACACGG 480


2144R-1.IR_full       481 CAGGTCGAGAACCATGATTCC 501
                          |||||||||||||||||||||
2144R-1.in silico     481 CAGGTCGAGAACCATGATTCC 501

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_136448.2	CG2144-RA (CG2144), mRNA
0	0	2	2	4	NM_137201.2	CG8187-RA (CG8187), mRNA
0	0	1	3	4	NM_001042801.1	CG34104-RB, transcript variant B (CG34104), mRNA
0	0	1	3	4	NM_001042802.1	CG34104-RA, transcript variant A (CG34104), mRNA
0	0	1	3	3	NM_139743.2	CG10483-RA (CG10483), mRNA
0	0	1	3	3	NM_001014727.1	CG12154-RB, transcript variant B (oc), mRNA
0	0	1	3	3	NM_078536.3	CG12154-RA, transcript variant A (oc), mRNA
0	0	1	3	3	NM_143844.2	CG17508-RA (CG17508), mRNA
0	0	1	3	2	NM_169510.1	CG8790-RB, transcript variant B (CG8790), mRNA
0	0	1	3	2	NM_142022.2	CG8790-RA, transcript variant A (CG8790), mRNA
0	0	1	2	4	NM_142590.1	CG4465-RA (CG4465), mRNA
0	0	0	5	4	NM_141866.2	CG14728-RA (sad), mRNA
0	0	0	5	3	NM_135867.2	CG7516-RA (CG7516), mRNA
0	0	0	5	2	NM_078831.1	CG5006-RA (Or33c), mRNA
0	0	0	4	6	NM_078748.2	CG12205-RA (Bsg25A), mRNA
0	0	0	4	6	NM_142819.1	CG6954-RA (CG6954), mRNA
0	0	0	4	4	NM_132127.2	CG4542-RA (CG4542), mRNA
0	0	0	4	4	NM_140877.2	CG9376-RA (CG9376), mRNA
0	0	0	4	3	NM_142382.1	CG14326-RA (CG14326), mRNA
0	0	0	3	7	NM_135393.2	CG13097-RA (CG13097), mRNA
0	0	0	3	6	NM_170286.1	CG31079-RA (CG31079), mRNA
0	0	0	3	6	NM_139735.2	CG5568-RA (CG5568), mRNA
0	0	0	3	6	NM_167686.1	CG11940-RB, transcript variant B (CG11940), mRNA
0	0	0	3	6	NM_134519.1	CG11940-RA, transcript variant A (CG11940), mRNA
0	0	0	3	5	NM_135390.2	CG13088-RA (CG13088), mRNA
0	0	0	3	5	NM_130487.2	CG18273-RA (CG18273), mRNA
0	0	0	3	5	NM_176099.1	CG33140-RA (CG33140), mRNA
0	0	0	3	5	NM_167640.1	CG7990-RB, transcript variant B (CG7990), mRNA
0	0	0	3	5	NM_133133.1	CG7990-RA, transcript variant A (CG7990), mRNA
0	0	0	3	5	NM_166870.1	CG32859-RA (eIF4E-7), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

dme00563 : Glycosylphosphatidylinositol (GPI)-anchor biosynthesis
dme01100 : Metabolic pathways

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