NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

2137R-2

Symbol

CG2137

Full Name

CG2137

CG No

CG2137

Old CG No

CG2137

Synonyms

146747_at, CG2137

Accession No (Link to NCBI)

NM_136451.1

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGCCTGGAGGCCTTTAAGCGAA

Primer Seq. 3'

aatctagaggtaccCATCCTGGCATCGTCGTGCTG

Predicted Fragment Size

IR fragment full Seq

0001 GCGGNACCNC TGNGGAGNCC TTTAAGCGAA CCCACTACGA CATCCTCATC ATTGgcGGCG 
0061 GCGCCGTGGG TTCCGGCTGT GCTCTCGATG CTGCCACGCG GGGCTTGAAG ACCGCCCTGG 
0121 TGGAAGCGAA TGACTTTGGC AGCGGCACTT CCTCCAAATC GAGCAAACTG TTGCACGGCG 
0181 GCCTCAGGGA TCTGGAGCAG GCCATCTCGC GTCTGGACGT GGCTGCCTTT CGGCGTGTTC 
0241 GCTGCTCCTT GCAGGAACGC AGCCACATTG GCAAGCTGGC GCCGCATCTT AGCCAGCCGG 
0301 TCCCCATCCT GCTGCCCGTC CACCACTGGT GGCAGGCTCC GCTCTACTAC ATGAAGCTAA 
0361 AGATTTACCA CCTGATGGCG CCCAAGGCCA CCAGGAGTTT TCAGTATCTG AGTGCCGACG 
0421 ACGCGTTGGA AGTATTTCCG ATGCTGCGAC GCCAGGAGCT GTTTTGGCGC CTTCGTCTTC 
0481 TACGAGGGTC AGCACGACGA TGCCAGGATG

in silico PCR Fragment

0001 GC-------C T--GGAGGCC TTTAAGCGAA CCCACTACGA CATCCTCATC ATTGGCGGCG 
0061 GCGCCGTGGG TTCCGGCTGT GCTCTCGATG CTGCCACGCG GGGCTTGAAG ACCGCCCTGG 
0121 TGGAAGCGAA TGACTTTGGC AGCGGCACTT CCTCCAAATC GAGCAAACTG TTGCACGGCG 
0181 GCCTCAGGGA TCTGGAGCAG GCCATCTCGC GTCTGGACGT GGCTGCCTTT CGGCGTGTTC 
0241 GCTGCTCCTT GCAGGAACGC AGCCACATTG GCAAGCTGGC GCCGCATCTT AGCCAGCCGG 
0301 TCCCCATCCT GCTGCCCGTC CACCACTGGT GGCAGGCTCC GCTCTACTAC ATGAAGCTAA 
0361 AGATTTACCA CCTGATGGCG CCCAAGGCCA CCAGGAGTTT TCAGTATCTG AGTGCCGACG 
0421 ACGCGTTGGA AGTATTTCCG ATGCTGCGAC GCCAGGAGCT G-TTTGGCGC CTTCGTCTTC 
0481 TACGAGGGTC AGCACGACGA TGCCAGGATG

Assemble Data

<PRE>

2137R-2.IR_full       1   GC<span class="snp"><tt>G</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>N</tt></span><span class="snp"><tt>A</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>N</tt></span>CT<span class="snp"><tt>G</tt></span><span class="snp"><tt>N</tt></span>GGAG<span class="snp"><tt>N</tt></span>CCTTTAAGCGAACCCACTACGACATCCTCATCATTGGCGGCG 60
                          ||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||
2137R-2.in silico     1   GC<span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span>CT<span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span>GGAG<span class="snp"><tt>G</tt></span>CCTTTAAGCGAACCCACTACGACATCCTCATCATTGGCGGCG 60


2137R-2.IR_full       61  GCGCCGTGGGTTCCGGCTGTGCTCTCGATGCTGCCACGCGGGGCTTGAAGACCGCCCTGG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2137R-2.in silico     61  GCGCCGTGGGTTCCGGCTGTGCTCTCGATGCTGCCACGCGGGGCTTGAAGACCGCCCTGG 120


2137R-2.IR_full       121 TGGAAGCGAATGACTTTGGCAGCGGCACTTCCTCCAAATCGAGCAAACTGTTGCACGGCG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2137R-2.in silico     121 TGGAAGCGAATGACTTTGGCAGCGGCACTTCCTCCAAATCGAGCAAACTGTTGCACGGCG 180


2137R-2.IR_full       181 GCCTCAGGGATCTGGAGCAGGCCATCTCGCGTCTGGACGTGGCTGCCTTTCGGCGTGTTC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2137R-2.in silico     181 GCCTCAGGGATCTGGAGCAGGCCATCTCGCGTCTGGACGTGGCTGCCTTTCGGCGTGTTC 240


2137R-2.IR_full       241 GCTGCTCCTTGCAGGAACGCAGCCACATTGGCAAGCTGGCGCCGCATCTTAGCCAGCCGG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2137R-2.in silico     241 GCTGCTCCTTGCAGGAACGCAGCCACATTGGCAAGCTGGCGCCGCATCTTAGCCAGCCGG 300


2137R-2.IR_full       301 TCCCCATCCTGCTGCCCGTCCACCACTGGTGGCAGGCTCCGCTCTACTACATGAAGCTAA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2137R-2.in silico     301 TCCCCATCCTGCTGCCCGTCCACCACTGGTGGCAGGCTCCGCTCTACTACATGAAGCTAA 360


2137R-2.IR_full       361 AGATTTACCACCTGATGGCGCCCAAGGCCACCAGGAGTTTTCAGTATCTGAGTGCCGACG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2137R-2.in silico     361 AGATTTACCACCTGATGGCGCCCAAGGCCACCAGGAGTTTTCAGTATCTGAGTGCCGACG 420


2137R-2.IR_full       421 ACGCGTTGGAAGTATTTCCGATGCTGCGACGCCAGGAGCTG<span class="snp"><tt>T</tt></span>TTTGGCGCCTTCGTCTTC 480
                          |||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||
2137R-2.in silico     421 ACGCGTTGGAAGTATTTCCGATGCTGCGACGCCAGGAGCTG<span class="snp"><tt>-</tt></span>TTTGGCGCCTTCGTCTTC 480


2137R-2.IR_full       481 TACGAGGGTCAGCACGACGATGCCAGGATG 510
                          ||||||||||||||||||||||||||||||
2137R-2.in silico     481 TACGAGGGTCAGCACGACGATGCCAGGATG 510

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_136451.1	CG2137-RA (CG2137), mRNA
0.2	1	2	2	4	NM_079255.2	CG6519-RA (Cp15), mRNA
0.2	1	2	2	3	NM_132288.1	CG15365-RA (CG15365), mRNA
0	0	7	2	2	NM_001043110.1	CG6883-RB, transcript variant B (trh), mRNA
0	0	7	2	2	NM_079148.2	CG6883-RA, transcript variant A (trh), mRNA
0	0	3	2	6	NM_132789.2	CG6170-RA, transcript variant A (HDAC6), mRNA
0	0	3	2	5	NM_140017.1	CG5644-RA (CG5644), mRNA
0	0	3	2	5	NM_167436.1	CG6170-RB, transcript variant B (HDAC6), mRNA
0	0	3	2	5	NM_167437.1	CG6170-RC, transcript variant C (HDAC6), mRNA
0	0	2	4	2	NM_137508.2	CG5345-RA (Eip55E), mRNA
0	0	2	3	10	NM_080530.2	CG10655-RA (l(2)37Bb), mRNA
0	0	2	3	10	NM_167852.1	CG9097-RB, transcript variant B (bab1), mRNA
0	0	2	3	4	NM_145873.2	CG18659-RA, transcript variant A (CG18659), mRNA
0	0	2	3	4	NM_136614.4	CG18659-RB, transcript variant B (CG18659), mRNA
0	0	2	2	7	NM_170563.1	CG1815-RB, transcript variant B (CG1815), mRNA
0	0	2	2	7	NM_143624.2	CG1815-RC, transcript variant C (CG1815), mRNA
0	0	2	2	7	NM_170562.1	CG1815-RA, transcript variant A (CG1815), mRNA
0	0	2	2	4	NM_079274.2	CG4167-RA (Hsp67Ba), mRNA
0	0	1	13	19	NM_166118.1	CG8256-RA, transcript variant A (l(2)k05713), mRNA
0	0	1	13	19	NM_166119.1	CG8256-RB, transcript variant B (l(2)k05713), mRNA
0	0	1	13	19	NM_137219.2	CG8256-RC, transcript variant C (l(2)k05713), mRNA
0	0	1	5	4	NM_140735.1	CG6311-RB (CG6311), mRNA
0	0	1	4	10	NM_206710.1	CG1372-RB, transcript variant B (yl), mRNA
0	0	1	4	10	NM_078596.2	CG1372-RA, transcript variant A (yl), mRNA
0	0	1	3	2	NM_142550.1	CG3517-RA (CG3517), mRNA
0	0	1	2	6	NM_001032403.1	CG7649-RB, transcript variant B (Neu3), mRNA
0	0	1	2	4	NM_143366.2	CG9986-RA (CG9986), mRNA
0	0	1	2	3	NM_137413.1	CG6401-RA (CG6401), mRNA
0	0	1	2	3	NM_132521.2	CG1578-RA (CG1578), mRNA
0	0	1	2	3	NM_134503.2	CG14231-RA (CG14231), mRNA