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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 2127R-3 
 Symbol CG2127  Full Name CG2127 
 CG No CG2127  Old CG No CG2127 
 Synonyms bs19a07.y1, NEST:bs19a07, anon-WO0140519.25, CG2127 
 Accession No (Link to NCBI) NM_136531.2 
 Inserted Chr. lll 
 Insertional Mutation  semi-lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		 
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGGGCACCATTTGCGATGCAGT 
 Primer Seq. 3' 
aatctagaggtaccCTGAGAAGCTGGGATACGGCG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GGGCACCATT TGCGATGCAG TCAAGGGCGG TAAGcGCAAG AAGAAGGAAG AGCCCAAGAA 
0061 GGAAAAGTCC AACAAGCCGA AGCTGCCAGC AAAGATGAGG TCCATGTGGT ATATACCCCG 
0121 ACTGCGAGTA CGTGCCAAAG TGCGATGTGC CAGTGCGGTA CGACATCCAG CACTACCGCA 
0181 TATCGGACAA AGAGGCCCGC CAGTACCAGG TGACGTGGAA CGAGTGCCCC CGGTTGGTGA 
0241 TCAAGCCCAA GAAGGTGTGC ATNCCACGCA AAGCGACCGC GTCCGAAGCC CTGCCGCCGT 
0301 CAGCGGAAGA CGGTGGTGGC CACAGCCCGT CCCACGATGC CCATGCTTAA TCCCATGGAG 
0361 TGCAAGAAGG CAGAGGACTC CTCACCCTGT CCGCGCTGGA CTCTTCCCTG CTGCAAGCCC 
0421 GCCCGTATTC CGCCCAGTTG CCACCGAGAA CGCCGACCTA CCGACTGTAC NANGCGcCCG 
0481 GCGCCGTATC CCAGCTTCTC AG 
 in silico PCR Fragment 
0001 GGGCACCATT TGCGATGCAG TCAAGGGCGG TAAGCGCAAG AAGAAGGAAG AGCCCAAGAA 
0061 GGAAAAGTCC AACAAGCCGA AGCTGCCAGC AAAGATGAGG TCCATGTGGT ATATA-CCCG 
0121 ACTGCGAGTA CGTGCCAAAG TGCGATGTGC CAGTGCGGTA CGACATCCAG CACTACCGCA 
0181 TATCGGACAA AGAGGCCCGC CAGTACCAGG TGACGTGGAA CGAGTGCCCC CGGTTGGTGA 
0241 TCAAGCCCAA GAAGGTGTGC AT-CCACGCA AAGCGACCGC GTCCGAAGCC CTGCCGCCGT 
0301 CAGCGGAAGA CGGTGGTGGC CACAGCCCGT CCCACGATGC CCATGCTTAA TCCCATGGAG 
0361 TGCAAGAAGG CAGAGGACTC CTCACCCTGT CCGCGCTGGA CTCTTCCCTG CTGCAAGCCC 
0421 GCCCGTATTC CGCCCAGTTG CCACCGAGAA CGCCGACCTA CCGACTGTAC CAAGCGCCCG 
0481 GCGCCGTATC CCAGCTTCTC AG 
 Assemble Data 
<PRE>

2127R-3.IR_full       1   GGGCACCATTTGCGATGCAGTCAAGGGCGGTAAGCGCAAGAAGAAGGAAGAGCCCAAGAA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2127R-3.in silico     1   GGGCACCATTTGCGATGCAGTCAAGGGCGGTAAGCGCAAGAAGAAGGAAGAGCCCAAGAA 60


2127R-3.IR_full       61  GGAAAAGTCCAACAAGCCGAAGCTGCCAGCAAAGATGAGGTCCATGTGGTATATA<span class="snp"><tt>C</tt></span>CCCG 120
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||
2127R-3.in silico     61  GGAAAAGTCCAACAAGCCGAAGCTGCCAGCAAAGATGAGGTCCATGTGGTATATA<span class="snp"><tt>-</tt></span>CCCG 120


2127R-3.IR_full       121 ACTGCGAGTACGTGCCAAAGTGCGATGTGCCAGTGCGGTACGACATCCAGCACTACCGCA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2127R-3.in silico     121 ACTGCGAGTACGTGCCAAAGTGCGATGTGCCAGTGCGGTACGACATCCAGCACTACCGCA 180


2127R-3.IR_full       181 TATCGGACAAAGAGGCCCGCCAGTACCAGGTGACGTGGAACGAGTGCCCCCGGTTGGTGA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2127R-3.in silico     181 TATCGGACAAAGAGGCCCGCCAGTACCAGGTGACGTGGAACGAGTGCCCCCGGTTGGTGA 240


2127R-3.IR_full       241 TCAAGCCCAAGAAGGTGTGCAT<span class="snp"><tt>N</tt></span>CCACGCAAAGCGACCGCGTCCGAAGCCCTGCCGCCGT 300
                          ||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||
2127R-3.in silico     241 TCAAGCCCAAGAAGGTGTGCAT<span class="snp"><tt>-</tt></span>CCACGCAAAGCGACCGCGTCCGAAGCCCTGCCGCCGT 300


2127R-3.IR_full       301 CAGCGGAAGACGGTGGTGGCCACAGCCCGTCCCACGATGCCCATGCTTAATCCCATGGAG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2127R-3.in silico     301 CAGCGGAAGACGGTGGTGGCCACAGCCCGTCCCACGATGCCCATGCTTAATCCCATGGAG 360


2127R-3.IR_full       361 TGCAAGAAGGCAGAGGACTCCTCACCCTGTCCGCGCTGGACTCTTCCCTGCTGCAAGCCC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2127R-3.in silico     361 TGCAAGAAGGCAGAGGACTCCTCACCCTGTCCGCGCTGGACTCTTCCCTGCTGCAAGCCC 420


2127R-3.IR_full       421 GCCCGTATTCCGCCCAGTTGCCACCGAGAACGCCGACCTACCGACTGTAC<span class="snp"><tt>N</tt></span>A<span class="snp"><tt>N</tt></span>GCGCCCG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|<span class="snp"><tt>&nbsp;</tt></span>|||||||
2127R-3.in silico     421 GCCCGTATTCCGCCCAGTTGCCACCGAGAACGCCGACCTACCGACTGTAC<span class="snp"><tt>C</tt></span>A<span class="snp"><tt>A</tt></span>GCGCCCG 480


2127R-3.IR_full       481 GCGCCGTATCCCAGCTTCTCAG 502
                          ||||||||||||||||||||||
2127R-3.in silico     481 GCGCCGTATCCCAGCTTCTCAG 502

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  6  NM_136531.2  CG2127-RA, transcript variant A (CG2127), mRNA 
100   482  0  0  6  NM_165607.1  CG2127-RB, transcript variant B (CG2127), mRNA 
0   0  7  4  6  NM_139541.1  CG12017-RA (CG12017), mRNA 
0   0  2  3  4  NM_080314.2  CG8590-RA (Klp3A), mRNA 
0   0  2  3  3  NM_057818.2  CG10637-RB, transcript variant B (Nak), mRNA 
0   0  2  3  3  NM_057817.2  CG10637-RA, transcript variant A (Nak), mRNA 
0   0  2  2  7  NM_132267.4  CG12109-RB (Caf1-180), mRNA 
0   0  2  2  5  NM_141388.2  CG1249-RA (CG1249), mRNA 
0   0  1  5  2  NM_078527.2  CG2151-RA, transcript variant A (Trxr-1), mRNA 
0   0  1  5  2  NM_167149.1  CG2151-RB, transcript variant B (Trxr-1), mRNA 
0   0  1  5  2  NM_167150.1  CG2151-RC, transcript variant C (Trxr-1), mRNA 
0   0  1  4  7  NM_135693.1  CG6766-RA (CG6766), mRNA 
0   0  1  4  5  NM_139688.2  CG4623-RA (CG4623), mRNA 
0   0  1  3  7  NM_079127.2  CG3691-RB, transcript variant B (Pof), mRNA 
0   0  1  3  7  NM_166682.1  CG3691-RA, transcript variant A (Pof), mRNA 
0   0  1  3  4  NM_078606.3  CG6146-RA, transcript variant A (Top1), mRNA 
0   0  1  3  4  NM_001014742.1  CG6146-RC, transcript variant C (Top1), mRNA 
0   0  1  2  7  NM_139680.1  CG17150-RA, transcript variant A (CG17150), mRNA 
0   0  1  2  6  NM_141432.1  CG1965-RA (CG1965), mRNA 
0   0  1  2  3  NM_136380.1  CG17266-RA (CG17266), mRNA 
0   0  1  2  2  NM_135331.1  CG7367-RA (CG7367), mRNA 
0   0  0  8  5  NM_132620.1  CG15720-RA, transcript variant A (CG15720), mRNA 
0   0  0  8  5  NM_001042809.1  CG15720-RB, transcript variant B (CG15720), mRNA 
0   0  0  7  9  NM_166957.1  CG32793-RA (CG32793), mRNA 
0   0  0  7  2  NM_169910.1  CG4141-RB, transcript variant B (Pi3K92E), mRNA 
0   0  0  7  2  NM_142645.2  CG4141-RA, transcript variant A (Pi3K92E), mRNA 
0   0  0  6  7  NM_206152.1  CG4847-RE, transcript variant E (CG4847), mRNA 
0   0  0  6  7  NM_166237.1  CG4847-RC, transcript variant C (CG4847), mRNA 
0   0  0  6  7  NM_137377.2  CG4847-RA, transcript variant A (CG4847), mRNA 
0   0  0  6  7  NM_166235.1  CG4847-RD, transcript variant D (CG4847), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway

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