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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 2121R-1 
 Symbol CG2121  Full Name CG2121 
 CG No CG2121  Old CG No CG2121 
 Synonyms CG8698, CG2121 
 Accession No (Link to NCBI) NM_136534.2 
 Inserted Chr. lll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		 
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgAGCTCACCCGGGACAACAACT 
 Primer Seq. 3' 
aatctagaggtaccAACAGGCCGAAGAACTTGACG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 AGCTCACCCG GGACAACAAC TGCTCCGAAC gGGGACAACA AAGTCAATCA GCGCCATTAC 
0061 GAGCCGGCCG AGCGCTTCAT CATCACCAAG AACGTGGTCG TGATAGGCCT GGCTTTCATG 
0121 ATTCACTTCA CGGCCTTCCA CGGCACATCG AATCTGCAGA GCTCGGTGAA CGCGGATAAG 
0181 GCCCTGGGCA CCACCACGCT TGCCGTCATC TACGGCTCGC TGATACTGTC CAACATCTTC 
0241 CTGCCGATGA CCGTGATAAG ATGGTTCGGT TGCCGGTTGA CCATGGCGCT GGCCCTCTTC 
0301 GCCTACATGC CCTACATAGC CGCCCAGTTT TACCCACGGT TCGAGACCCT AATCCCGGCG 
0361 GCCCTGATGG TGGGTTTCGG TGGCGGGCCA TTGTGGTGCT CCAAGTGCAC CTACCTATCC 
0421 ACCGTCTCCG AGGCACTTAC CCAGGTGCGG GGCAACAAGT CGCGTAAGGA TGTGAACACC 
0481 GTCAAGTTCT TCGGCCTGTT  
 in silico PCR Fragment 
0001 AGCTCACCCG GGACAACAAC TGCTCCGAAC GGGGACAACA AAGTCAATCA GCGCCATTAC 
0061 GAGCCGGCCG AGCGCTTCAT CATCACCAAG AACGTGGTCG TGATAGGCCT GGCTTTCATG 
0121 ATTCACTTCA CGGCCTTCCA CGGCACATCG AATCTGCAGA GCTCGGTGAA CGCGGATAAG 
0181 GCCCTGGGCA CCACCACGCT TGCCGTCATC TACGGCTCGC TGATACTGTC CAACATCTTC 
0241 CTGCCGATGA CCGTGATAAG ATGGTTCGGT TGCCGGTTGA CCATGGCGCT GGCCCTCTTC 
0301 GCCTACATGC CCTACATAGC CGCCCAGTTT TACCCACGGT TCGAGACCCT AATCCCGGCG 
0361 GCCCTGATGG TGGGTTTCGG TGGCGGGCCA TTGTGGTGCT CCAAGTGCAC CTACCTATCC 
0421 ACCGTCTCCG AGGCACTTAC CCAGGTGCGG GGCAACAAGT CGCGTAAGGA TGTGAACACC 
0481 GTCAAGTTCT TCGGCCTGTT  
 Assemble Data 
<PRE>

2121R-1.IR_full       1   AGCTCACCCGGGACAACAACTGCTCCGAACGGGGACAACAAAGTCAATCAGCGCCATTAC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2121R-1.in silico     1   AGCTCACCCGGGACAACAACTGCTCCGAACGGGGACAACAAAGTCAATCAGCGCCATTAC 60


2121R-1.IR_full       61  GAGCCGGCCGAGCGCTTCATCATCACCAAGAACGTGGTCGTGATAGGCCTGGCTTTCATG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2121R-1.in silico     61  GAGCCGGCCGAGCGCTTCATCATCACCAAGAACGTGGTCGTGATAGGCCTGGCTTTCATG 120


2121R-1.IR_full       121 ATTCACTTCACGGCCTTCCACGGCACATCGAATCTGCAGAGCTCGGTGAACGCGGATAAG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2121R-1.in silico     121 ATTCACTTCACGGCCTTCCACGGCACATCGAATCTGCAGAGCTCGGTGAACGCGGATAAG 180


2121R-1.IR_full       181 GCCCTGGGCACCACCACGCTTGCCGTCATCTACGGCTCGCTGATACTGTCCAACATCTTC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2121R-1.in silico     181 GCCCTGGGCACCACCACGCTTGCCGTCATCTACGGCTCGCTGATACTGTCCAACATCTTC 240


2121R-1.IR_full       241 CTGCCGATGACCGTGATAAGATGGTTCGGTTGCCGGTTGACCATGGCGCTGGCCCTCTTC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2121R-1.in silico     241 CTGCCGATGACCGTGATAAGATGGTTCGGTTGCCGGTTGACCATGGCGCTGGCCCTCTTC 300


2121R-1.IR_full       301 GCCTACATGCCCTACATAGCCGCCCAGTTTTACCCACGGTTCGAGACCCTAATCCCGGCG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2121R-1.in silico     301 GCCTACATGCCCTACATAGCCGCCCAGTTTTACCCACGGTTCGAGACCCTAATCCCGGCG 360


2121R-1.IR_full       361 GCCCTGATGGTGGGTTTCGGTGGCGGGCCATTGTGGTGCTCCAAGTGCACCTACCTATCC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2121R-1.in silico     361 GCCCTGATGGTGGGTTTCGGTGGCGGGCCATTGTGGTGCTCCAAGTGCACCTACCTATCC 420


2121R-1.IR_full       421 ACCGTCTCCGAGGCACTTACCCAGGTGCGGGGCAACAAGTCGCGTAAGGATGTGAACACC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2121R-1.in silico     421 ACCGTCTCCGAGGCACTTACCCAGGTGCGGGGCAACAAGTCGCGTAAGGATGTGAACACC 480


2121R-1.IR_full       481 GTCAAGTTCTTCGGCCTGTT 500
                          ||||||||||||||||||||
2121R-1.in silico     481 GTCAAGTTCTTCGGCCTGTT 500

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  4  NM_136534.2  CG2121-RA (CG2121), mRNA 
0.2   1  4  2  2  NM_137934.2  CG9861-RA (CG9861), mRNA 
0   0  5  3  3  NM_168448.1  CG32082-RA (CG32082), mRNA 
0   0  3  5  2  NM_176408.1  CG33097-RA, transcript variant A (CG33097), mRNA 
0   0  3  5  2  NM_001043218.1  CG33097-RB, transcript variant B (CG33097), mRNA 
0   0  2  3  2  NM_134490.2  CG12238-RA (l(1)G0084), mRNA 
0   0  2  3  2  NM_079255.2  CG6519-RA (Cp15), mRNA 
0   0  2  2  4  NM_167021.1  CG2861-RB, transcript variant B (CG2861), mRNA 
0   0  2  2  4  NM_131961.1  CG2861-RA, transcript variant A (CG2861), mRNA 
0   0  2  2  3  NM_057400.3  CG6993-RA (ss), mRNA 
0   0  1  4  4  NM_143068.1  CG11786-RA (CG11786), mRNA 
0   0  1  4  3  NM_140972.2  CG5932-RA (CG5932), mRNA 
0   0  1  4  2  NM_139507.2  CG16753-RA (CG16753), mRNA 
0   0  1  3  4  NM_170091.1  CG10217-RB, transcript variant B (CG10217), mRNA 
0   0  1  3  4  NM_142925.1  CG10217-RA, transcript variant A (CG10217), mRNA 
0   0  1  3  4  NM_057410.3  CG10079-RA, transcript variant A (Egfr), mRNA 
0   0  1  3  4  NM_057411.3  CG10079-RB, transcript variant B (Egfr), mRNA 
0   0  1  3  3  NM_136704.2  CG2264-RB, transcript variant B (CG2264), mRNA 
0   0  1  3  2  NM_142915.2  CG31148-RA (CG31148), mRNA 
0   0  1  2  7  NM_206765.1  CG18572-RB, transcript variant B (r), mRNA 
0   0  1  2  7  NM_078653.1  CG18572-RA, transcript variant A (r), mRNA 
0   0  1  2  6  NM_165983.1  CG18278-RA (CG18278), mRNA 
0   0  1  2  5  NM_137028.2  CG30059-RA (CG30059), mRNA 
0   0  0  5  7  NM_137914.2  CG30330-RA (Gr59d), mRNA 
0   0  0  5  3  NM_140882.2  CG8782-RA (Oat), mRNA 
0   0  0  5  2  NM_164701.1  CG11328-RA, transcript variant A (Nhe3), mRNA 
0   0  0  5  2  NM_135220.2  CG11328-RB, transcript variant B (Nhe3), mRNA 
0   0  0  4  4  NM_170398.1  CG14516-RA, transcript variant A (CG14516), mRNA 
0   0  0  4  4  NM_143417.2  CG14516-RB, transcript variant B (CG14516), mRNA 
0   0  0  4  4  NM_080495.3  CG12047-RC, transcript variant C (mud), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway

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