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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

18808R-1

Symbol

CG18808

Full Name

CG18808

CG No

CG18808

Old CG No

CG18808

Synonyms

CG18808

Accession No (Link to NCBI)

NM_144411.1

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAAGCTGCCTTCGTGGCTATCA

Primer Seq. 3'

aatctagaggtaccTGGGACCCATCATCGTTGGAG

Predicted Fragment Size

IR fragment full Seq

0001 AAGCTGCCTT CGTGGCTATC ACATNGCATG CCGCCTCTAC GCAGCAGCGG GGCGACACGT 
0061 CCTTGGTTCT GGTTCTGGTG GATGAGGCCC GCCGCNTTTA TCCAGGACTG CCTGCTACGG 
0121 GTTGGAGTTT CCCCCTCCAA GGTGCGCTGC ATCAGCGAGT TCCTGGTTGT GGCGGACTAT 
0181 CGAGGAAACT ACGGAAGCGG GCTGAATCGC CTGGATTACT CATCTCAGTG ATCTGCAGAG 
0241 CGGGCATGCC AAGGTGGGCG CAGAGCCTTC AATAATCAGT GAAACGGTAG TCCACCGCCC 
0301 ATGTAAATGG CAATTCCGCC TTGGGTGTGT TTGTGGGAAA CTTTTGTATG GNATTTGGCG 
0361 GTGAAGAAAG CTGAAGACTC TGGCATCGGT TTCGTAGTGG CCCAGCAATC TCACGACATT 
0421 GGCATGGCCA GCTGGTTTAC ATTTCGCGCT GCCGGCAAGG GATTAGCCGG AATCGTGATG 
0481 TCCAATTCGG CTCCAACGAT GATGGGTCCC A

in silico PCR Fragment

0001 AAGCTGCCTT CGTGGCTATC ACAT-GCATG CCGCCTCTAC GCAGCAGCGG GGCGACACGT 
0061 CCT------T GGTTCTGGTG GATGAGGCCC GCCGC-TTTA TCCAGGACTG CCTGCTACGG 
0121 GTTGGAGTTT CCCCCTCCAA GGTGCGCTGC ATCAGCGAGT TCCTGGTTGT GGCGGACTAT 
0181 CGAGGAAACT ACGGAAGCGG GCTGAATCGC CTGGATTACT -ATCTCAGTG ATCTGCAGAG 
0241 CGGGCATGCC AAGGTGGGCG CAGAGCCTTC AATAATCAGT GAAACGGT-G TCCACCGCCC 
0301 ATGTAAATGG CAATTCCGCC TTGGGTGTGT TTGTGGGAAA CTTTTGTATG G-ATTTGGCG 
0361 GTGAAGAAAG CTGAAGACTC TGGCATCGGT TTCGTAGTGG CCCAGCAATC TCACGACATT 
0421 GGCATGGCCA GCTGGTTTAC ATTTCGCGCT GCCGGCAAGG GATTAGCCGG AATCGTGATG 
0481 TCCAATTCGG CTCCAACGAT GATGGGTCCC A

Assemble Data

<PRE>

18808R-1.IR_full       1   AAGCTGCCTTCGTGGCTATCACAT<span class="snp"><tt>N</tt></span>GCATGCCGCCTCTACGCAGCAGCGGGGCGACACGT 60
                           ||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||
18808R-1.in silico     1   AAGCTGCCTTCGTGGCTATCACAT<span class="snp"><tt>-</tt></span>GCATGCCGCCTCTACGCAGCAGCGGGGCGACACGT 60


18808R-1.IR_full       61  CCT<span class="snp"><tt>T</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>T</tt></span><span class="snp"><tt>T</tt></span><span class="snp"><tt>C</tt></span>TGGTTCTGGTGGATGAGGCCCGCCGC<span class="snp"><tt>N</tt></span>TTTATCCAGGACTGCCTGCTACGG 120
                           |||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||
18808R-1.in silico     61  CCT<span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span>TGGTTCTGGTGGATGAGGCCCGCCGC<span class="snp"><tt>-</tt></span>TTTATCCAGGACTGCCTGCTACGG 120


18808R-1.IR_full       121 GTTGGAGTTTCCCCCTCCAAGGTGCGCTGCATCAGCGAGTTCCTGGTTGTGGCGGACTAT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18808R-1.in silico     121 GTTGGAGTTTCCCCCTCCAAGGTGCGCTGCATCAGCGAGTTCCTGGTTGTGGCGGACTAT 180


18808R-1.IR_full       181 CGAGGAAACTACGGAAGCGGGCTGAATCGCCTGGATTACT<span class="snp"><tt>C</tt></span>ATCTCAGTGATCTGCAGAG 240
                           ||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||
18808R-1.in silico     181 CGAGGAAACTACGGAAGCGGGCTGAATCGCCTGGATTACT<span class="snp"><tt>-</tt></span>ATCTCAGTGATCTGCAGAG 240


18808R-1.IR_full       241 CGGGCATGCCAAGGTGGGCGCAGAGCCTTCAATAATCAGTGAAACGGT<span class="snp"><tt>A</tt></span>GTCCACCGCCC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||
18808R-1.in silico     241 CGGGCATGCCAAGGTGGGCGCAGAGCCTTCAATAATCAGTGAAACGGT<span class="snp"><tt>-</tt></span>GTCCACCGCCC 300


18808R-1.IR_full       301 ATGTAAATGGCAATTCCGCCTTGGGTGTGTTTGTGGGAAACTTTTGTATGG<span class="snp"><tt>N</tt></span>ATTTGGCG 360
                           |||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||
18808R-1.in silico     301 ATGTAAATGGCAATTCCGCCTTGGGTGTGTTTGTGGGAAACTTTTGTATGG<span class="snp"><tt>-</tt></span>ATTTGGCG 360


18808R-1.IR_full       361 GTGAAGAAAGCTGAAGACTCTGGCATCGGTTTCGTAGTGGCCCAGCAATCTCACGACATT 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18808R-1.in silico     361 GTGAAGAAAGCTGAAGACTCTGGCATCGGTTTCGTAGTGGCCCAGCAATCTCACGACATT 420


18808R-1.IR_full       421 GGCATGGCCAGCTGGTTTACATTTCGCGCTGCCGGCAAGGGATTAGCCGGAATCGTGATG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18808R-1.in silico     421 GGCATGGCCAGCTGGTTTACATTTCGCGCTGCCGGCAAGGGATTAGCCGGAATCGTGATG 480


18808R-1.IR_full       481 TCCAATTCGGCTCCAACGATGATGGGTCCCA 511
                           |||||||||||||||||||||||||||||||
18808R-1.in silico     481 TCCAATTCGGCTCCAACGATGATGGGTCCCA 511

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_144411.1	CG18808-RA (CG18808), mRNA
0	0	2	3	4	NM_078499.3	CG4690-RA (Tsp5D), mRNA
0	0	2	2	3	NM_139723.1	CG17795-RA, transcript variant A (mthl2), mRNA
0	0	2	2	3	NM_176284.1	CG17795-RB, transcript variant B (mthl2), mRNA
0	0	1	3	4	NM_135168.3	CG9491-RA (Gef26), mRNA
0	0	1	2	5	NM_057646.2	CG10844-RD, transcript variant D (Rya-r44F), mRNA
0	0	1	2	5	NM_057645.2	CG10844-RC, transcript variant C (Rya-r44F), mRNA
0	0	1	2	5	NM_057644.2	CG10844-RB, transcript variant B (Rya-r44F), mRNA
0	0	1	2	5	NM_057643.2	CG10844-RA, transcript variant A (Rya-r44F), mRNA
0	0	1	2	3	NM_165611.1	CG8643-RA (rgr), mRNA
0	0	1	2	3	NM_143808.2	CG5707-RA (CG5707), mRNA
0	0	1	2	3	NM_168038.1	CG32264-RD, transcript variant D (CG32264), mRNA
0	0	0	11	33	NM_001032052.1	CG33715-RE, transcript variant E (Msp-300), mRNA
0	0	0	5	11	NM_143112.1	CG11878-RA (CG11878), mRNA
0	0	0	5	4	NM_139619.1	CG1332-RA (CG1332), mRNA
0	0	0	5	2	NM_137830.1	CG3701-RA (CG3701), mRNA
0	0	0	4	7	NM_078717.2	CG3696-RA, transcript variant A (kis), mRNA
0	0	0	4	6	NM_079953.3	CG10776-RA (wit), mRNA
0	0	0	4	3	NM_168207.1	CG8588-RB, transcript variant B (pst), mRNA
0	0	0	4	3	NM_139853.1	CG8588-RA, transcript variant A (pst), mRNA
0	0	0	4	3	NM_168209.1	CG8588-RD, transcript variant D (pst), mRNA
0	0	0	4	3	NM_168208.1	CG8588-RC, transcript variant C (pst), mRNA
0	0	0	4	2	NM_080486.2	CG1966-RA (Acf1), mRNA
0	0	0	4	2	NM_001043139.1	CG11282-RC, transcript variant C (caps), mRNA
0	0	0	4	2	NM_141363.1	CG15589-RA (CG15589), mRNA
0	0	0	4	2	NM_166704.1	CG15792-RB, transcript variant B (zip), mRNA
0	0	0	4	2	NM_001014553.1	CG15792-RC, transcript variant C (zip), mRNA
0	0	0	4	2	NM_079136.2	CG15792-RA, transcript variant A (zip), mRNA
0	0	0	4	2	NM_001014552.1	CG15792-RD, transcript variant D (zip), mRNA
0	0	0	4	2	NM_132394.2	CG17841-RA (CG17841), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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