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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 18610R-3

Symbol CG30122 Full Name CG30122

CG No CG30122 Old CG No CG18610

Synonyms LD11002, CG18610, CG5477, CG30122

Accession No (Link to NCBI) NM_137510.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
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Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCTTCAAGCGAATTGCGGTCGT

Primer Seq. 3'

aatctagaggtaccGCACCCAATTTTGCTGGCCAC

Predicted Fragment Size

IR fragment full Seq

0001 CTTCAAGCGA ATTGCGGTTC GTTTGCATAC CCAGCGAAGA TGAATTGAAA CGTCGCATCG 
0061 CCGAAAAAGA GGAAAAGGGA AATGCTTTTA CAGTTAAAGA ATCAACAATT AATAATTTAC 
0121 GAGCCAACTT CACACTTCCC TCGCTGGAGT TTGGCTGGTT CGATGATATA AACTACACGG 
0181 AGCTGACTGG TGACGAGGCC AAGAGCGAGG TCAAGAAGTA CAACGAGAAG GGCAAAAAGG 
0241 CCATTGACGC GGAGAGGTCG CGTGACAAGA GATCCCGAGG AAGTCGCGAC AACTATCGTC 
0301 GTGATGACCG GAACCGGAAT CGATACAATG ATGACCGACG TCGTGATTAT GGCGGCCAGC 
0361 GGCACGAAAG TCGTTGGAGC GACTCCCGCA GGGGAGGCGG CGGTGGCGGT TACTCCAGCA 
0421 ATAGTGGTGG AGGCGGCAGT CGTGGCTACG ACAATCGCCG CAACTACAAC AGCGGTAGCG 
0481 GTGGCCAGCA AAAATTGGGT GC

in silico PCR Fragment

0001 CTTCAAGCGA ATTGCGG-TC GTTTGCATAC CCAGCGAAGA TGAATTGAAA CGTCGCATCG 
0061 CCGAAAAAGA GGAAAAGGGA AATGCTTTTA CAGTTAAAGA ATCAACAATT AATAATTTAC 
0121 GAGCCAACTT CACACTTCCC TCGCTGGAGT TTGGCTGGTT CGATGATATA AACTACACGG 
0181 AGCTGACTGG TGACGAGGCC AAGAGCGAGG TCAAGAAGTA CAACGAGAAG GGCAAAAAGG 
0241 CCATTGACGC GGAGAGGTCG CGTGACAAGA GATCCCGAGG AAGTCGCGAC AACTATCGTC 
0301 GTGATGACCG GAACCGGAAT CGATACAATG ATGACCGACG TCGTGATTAT GGCGGCCAGC 
0361 GGCACGAAAG TCGTTGGAGC GACTCCCGCA GGGGAGGCGG CGGTGGCGGT TACTCCAGCA 
0421 ATAGTGGTGG AGGCGGCAGT CGTGGCTACG ACAATCGCCG CAACTACAAC AGCGGTAGCG 
0481 GTGGCCAGC- AAAATTGGGT GC

Assemble Data


18610R-3.IR_full       1   CTTCAAGCGAATTGCGGTTCGTTTGCATACCCAGCGAAGATGAATTGAAACGTCGCATCG 60
                           ||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||
18610R-3.in silico     1   CTTCAAGCGAATTGCGG-TCGTTTGCATACCCAGCGAAGATGAATTGAAACGTCGCATCG 60


18610R-3.IR_full       61  CCGAAAAAGAGGAAAAGGGAAATGCTTTTACAGTTAAAGAATCAACAATTAATAATTTAC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18610R-3.in silico     61  CCGAAAAAGAGGAAAAGGGAAATGCTTTTACAGTTAAAGAATCAACAATTAATAATTTAC 120


18610R-3.IR_full       121 GAGCCAACTTCACACTTCCCTCGCTGGAGTTTGGCTGGTTCGATGATATAAACTACACGG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18610R-3.in silico     121 GAGCCAACTTCACACTTCCCTCGCTGGAGTTTGGCTGGTTCGATGATATAAACTACACGG 180


18610R-3.IR_full       181 AGCTGACTGGTGACGAGGCCAAGAGCGAGGTCAAGAAGTACAACGAGAAGGGCAAAAAGG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18610R-3.in silico     181 AGCTGACTGGTGACGAGGCCAAGAGCGAGGTCAAGAAGTACAACGAGAAGGGCAAAAAGG 240


18610R-3.IR_full       241 CCATTGACGCGGAGAGGTCGCGTGACAAGAGATCCCGAGGAAGTCGCGACAACTATCGTC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18610R-3.in silico     241 CCATTGACGCGGAGAGGTCGCGTGACAAGAGATCCCGAGGAAGTCGCGACAACTATCGTC 300


18610R-3.IR_full       301 GTGATGACCGGAACCGGAATCGATACAATGATGACCGACGTCGTGATTATGGCGGCCAGC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18610R-3.in silico     301 GTGATGACCGGAACCGGAATCGATACAATGATGACCGACGTCGTGATTATGGCGGCCAGC 360


18610R-3.IR_full       361 GGCACGAAAGTCGTTGGAGCGACTCCCGCAGGGGAGGCGGCGGTGGCGGTTACTCCAGCA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18610R-3.in silico     361 GGCACGAAAGTCGTTGGAGCGACTCCCGCAGGGGAGGCGGCGGTGGCGGTTACTCCAGCA 420


18610R-3.IR_full       421 ATAGTGGTGGAGGCGGCAGTCGTGGCTACGACAATCGCCGCAACTACAACAGCGGTAGCG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18610R-3.in silico     421 ATAGTGGTGGAGGCGGCAGTCGTGGCTACGACAATCGCCGCAACTACAACAGCGGTAGCG 480


18610R-3.IR_full       481 GTGGCCAGCAAAAATTGGGTGC 502
                           ||||||||| ||||||||||||
18610R-3.in silico     481 GTGGCCAGC-AAAATTGGGTGC 502

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	10	8	18	NM_137510.2	CG30122-RB (CG30122), mRNA
0.2	1	2	11	50	NM_167493.1	CG3606-RA, transcript variant A (caz), mRNA
0.2	1	2	11	50	NM_078641.2	CG3606-RB, transcript variant B (caz), mRNA
0	0	6	4	4	NM_206382.1	CG5151-RB, transcript variant B (CG5151), mRNA
0	0	6	4	4	NM_140587.1	CG5151-RA, transcript variant A (CG5151), mRNA
0	0	4	4	28	NM_135595.2	CG17108-RA (CG17108), mRNA
0	0	3	4	5	NM_135607.2	CG6495-RA (CG6495), mRNA
0	0	3	3	19	NM_057695.3	CG4038-RA (CG4038), mRNA
0	0	3	2	3	NM_079706.2	CG3412-RA (slmb), mRNA
0	0	2	5	2	NM_001042873.1	CG31651-RB, transcript variant B (pgant5), mRNA
0	0	2	5	2	NM_135062.3	CG31651-RA, transcript variant A (pgant5), mRNA
0	0	2	4	13	NM_142942.3	CG31140-RA, transcript variant A (CG31140), mRNA
0	0	2	4	12	NM_206553.1	CG31140-RB, transcript variant B (CG31140), mRNA
0	0	2	4	8	NM_132193.1	CG1514-RA (CG1514), mRNA
0	0	2	3	8	NM_166413.2	CG10052-RA (Rx), mRNA
0	0	2	3	6	NM_143717.1	CG10840-RB (eIF5B), mRNA
0	0	2	2	3	NM_142382.1	CG14326-RA (CG14326), mRNA
0	0	2	2	2	NM_136761.2	CG7614-RA (Mat1), mRNA
0	0	2	2	2	NM_132476.2	CG1657-RA (CG1657), mRNA
0	0	1	5	19	NM_141642.1	CG9373-RA (CG9373), mRNA
0	0	1	4	16	NM_136816.1	CG13214-RA, transcript variant A (CG13214), mRNA
0	0	1	4	3	NM_137767.2	CG4386-RA (CG4386), mRNA
0	0	1	3	10	NM_132953.1	CG13001-RA (CG13001), mRNA
0	0	1	3	9	NM_079962.3	CG18042-RA (lmg), mRNA
0	0	1	3	3	NM_206095.1	CG13214-RB, transcript variant B (CG13214), mRNA
0	0	1	3	2	NM_165832.1	CG30026-RA (CG30026), mRNA
0	0	1	2	11	NM_168102.1	CG32239-RA (Gef64C), mRNA
0	0	1	2	9	NM_078670.2	CG7092-RA (Dhc16F), mRNA
0	0	1	2	8	NM_170646.3	CG31349-RE, transcript variant E (pyd), mRNA
0	0	1	2	8	NM_132386.2	CG2961-RA (Ipod), mRNA

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