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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 1857R-1

Symbol nec Full Name necrotic

CG No CG1857 Old CG No CG1857

Synonyms Spn43Ac, CG1857, SER3, Ser3, anon-WO0118057.3, nec, Nec

Accession No (Link to NCBI) NM_080112.1

Inserted Chr. X

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
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Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCTAACCGTCCATCTTCTGGCT

Primer Seq. 3'

aatctagaggtaccTTGTACTTGATCACGCTCTCG

Predicted Fragment Size

IR fragment full Seq

0001 NTAACCGTCC ATCTTCTGGC TGCTCAGACC TTCGCCCAGG AGCTCATCGC TTGGCAACGG 
0061 CAACAACAAC AGCAGCAACA GCAGCAACTG CAGCTACAGC AGCAAANCTG CTGcTGCAGC 
0121 AGCAACAACA CCAACGTAAC CCAAGACCGG AGCTGGGCCT CCGTTCCCTG CCCGGAAACC 
0181 CGTGGACCCA GAACAATCAG GAAGCCATAA GCGATGTGGT GGCGGTGGAC CTAACCAAAC 
0241 GTGAGCCGGT CACTCCGCCA CCCAATCGCC CGCCGCCCGT CTTCAGCTAC ATGGACCGCT 
0301 TCAGCTCCGA GCTCTTCAAG GAGATCATTA AGTCGCAAAG TCAGCAGAAC GTGGTGTTCT 
0361 CGCCCTTCTC CGTCCACGCG CTGCTGGCCC TGATCTACGG GGCCTCGGAC GGAAAAACGT 
0421 TCCGGGAACT GCAGAAGGCC GGAGAGTTCA GCAAGAACGC CATGGCCGTG GCCCAGGACT 
0481 TCGAGAGCGT GATCAAGTAC AA

in silico PCR Fragment

0001 CTAACCGTCC ATCTTCTGGC TGCTCAGACC TTCGCCCAGG AGCTCATCGC TTGGCAACGG 
0061 CAACAACAAC AGCAGCAACA GCAGCAACTG CAGCTACAGC AGCAA--CTG CTGCTGCAGC 
0121 AGCAACAACA CCAACGTAAC CCAAGACCGG AGCTGGGCCT CCGTTCCCTG CCCGGAAACC 
0181 CGTGGACCCA GAACAATCAG GAAGCCATAA GCGATGTGGT GGCGGTGGAC CTAACCAAAC 
0241 GTGAGCCGGT CACTCCGCCA CCCAATCGCC CGCCGCCCGT CTTCAGCTAC ATGGACCGCT 
0301 TCAGCTCCGA GCTCTTCAAG GAGATCATTA AGTCGCAAAG TCAGCAGAAC GTGGTGTTCT 
0361 CGCCCTTCTC CGTCCACGCG CTGCTGGCCC TGATCTACGG GGCCTCGGAC GGAAAAACGT 
0421 TCCGGGAACT GCAGAAGGCC GGAGAGTTCA GCAAGAACGC CATGGCCGTG GCCCAGGACT 
0481 TCGAGAGCGT GATCAAGTAC AA

Assemble Data


1857R-1.IR_full        1   TAACCGTCCATCTTCTGGCTGCTCAGACCTTCGCCCAGGAGCTCATCGCTTGGCAACGG 59
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1857R-1.in silico      1   TAACCGTCCATCTTCTGGCTGCTCAGACCTTCGCCCAGGAGCTCATCGCTTGGCAACGG 59


1857R-1.IR_full       61  CAACAACAACAGCAGCAACAGCAGCAACTGCAGCTACAGCAGCAAANCTGCTGCTGCAGC 119
                          |||||||||||||||||||||||||||||||||||||||||||||  |||||||||||||
1857R-1.in silico     61  CAACAACAACAGCAGCAACAGCAGCAACTGCAGCTACAGCAGCAA--CTGCTGCTGCAGC 119


1857R-1.IR_full       121 AGCAACAACACCAACGTAACCCAAGACCGGAGCTGGGCCTCCGTTCCCTGCCCGGAAACC 179
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1857R-1.in silico     121 AGCAACAACACCAACGTAACCCAAGACCGGAGCTGGGCCTCCGTTCCCTGCCCGGAAACC 179


1857R-1.IR_full       181 CGTGGACCCAGAACAATCAGGAAGCCATAAGCGATGTGGTGGCGGTGGACCTAACCAAAC 239
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1857R-1.in silico     181 CGTGGACCCAGAACAATCAGGAAGCCATAAGCGATGTGGTGGCGGTGGACCTAACCAAAC 239


1857R-1.IR_full       241 GTGAGCCGGTCACTCCGCCACCCAATCGCCCGCCGCCCGTCTTCAGCTACATGGACCGCT 299
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1857R-1.in silico     241 GTGAGCCGGTCACTCCGCCACCCAATCGCCCGCCGCCCGTCTTCAGCTACATGGACCGCT 299


1857R-1.IR_full       301 TCAGCTCCGAGCTCTTCAAGGAGATCATTAAGTCGCAAAGTCAGCAGAACGTGGTGTTCT 359
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1857R-1.in silico     301 TCAGCTCCGAGCTCTTCAAGGAGATCATTAAGTCGCAAAGTCAGCAGAACGTGGTGTTCT 359


1857R-1.IR_full       361 CGCCCTTCTCCGTCCACGCGCTGCTGGCCCTGATCTACGGGGCCTCGGACGGAAAAACGT 419
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1857R-1.in silico     361 CGCCCTTCTCCGTCCACGCGCTGCTGGCCCTGATCTACGGGGCCTCGGACGGAAAAACGT 419


1857R-1.IR_full       421 TCCGGGAACTGCAGAAGGCCGGAGAGTTCAGCAAGAACGCCATGGCCGTGGCCCAGGACT 479
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1857R-1.in silico     421 TCCGGGAACTGCAGAAGGCCGGAGAGTTCAGCAAGAACGCCATGGCCGTGGCCCAGGACT 479


1857R-1.IR_full       481 TCGAGAGCGTGATCAAGTACAA 501
                          ||||||||||||||||||||||
1857R-1.in silico     481 TCGAGAGCGTGATCAAGTACAA 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	6	60	69	NM_080112.1	CG1857-RA (nec), mRNA
4.56	22	59	125	137	NM_167617.1	CG32548-RB, transcript variant B (CG32548), mRNA
4.56	22	59	125	131	NM_133065.2	CG32548-RC, transcript variant C (CG32548), mRNA
3.52	17	39	96	172	NM_137027.1	CG4744-RA (CG4744), mRNA
3.11	15	173	784	2358	NM_167335.1	CG4013-RC, transcript variant C (Smr), mRNA
3.11	15	173	784	2358	NM_167334.1	CG4013-RB, transcript variant B (Smr), mRNA
3.11	15	173	784	2358	NM_080536.2	CG4013-RA, transcript variant A (Smr), mRNA
2.48	12	30	195	660	NM_132288.1	CG15365-RA (CG15365), mRNA
2.07	10	32	152	487	NM_001014576.1	CG7391-RD, transcript variant D (Clk), mRNA
2.07	10	32	152	487	NM_001014575.1	CG7391-RE, transcript variant E (Clk), mRNA
2.07	10	32	152	487	NM_001014574.1	CG7391-RF, transcript variant F (Clk), mRNA
2.07	10	32	152	487	NM_079240.2	CG7391-RA, transcript variant A (Clk), mRNA
2.07	10	32	152	487	NM_206299.1	CG7391-RC, transcript variant C (Clk), mRNA
2.07	10	32	152	487	NM_170628.1	CG7391-RB, transcript variant B (Clk), mRNA
2.07	10	23	63	207	NM_078545.2	CG12653-RA (btd), mRNA
1.86	9	19	187	477	NM_079845.2	CG7951-RA (sima), mRNA
1.65	8	32	124	328	NM_142209.2	CG5166-RA, transcript variant A (Atx2), mRNA
1.65	8	32	124	328	NM_169657.1	CG5166-RB, transcript variant B (Atx2), mRNA
1.65	8	32	124	328	NM_169658.2	CG5166-RC, transcript variant C (Atx2), mRNA
1.45	7	57	315	927	NM_135077.2	CG14023-RA (CG14023), mRNA
1.45	7	46	238	873	NM_001038734.1	CG16902-RC (Hr4), mRNA
1.45	7	40	108	434	NM_165035.1	CG6043-RB, transcript variant B (CG6043), mRNA
1.45	7	40	108	434	NM_165037.1	CG6043-RC, transcript variant C (CG6043), mRNA
1.45	7	40	108	434	NM_165034.1	CG6043-RA, transcript variant A (CG6043), mRNA
1.45	7	40	108	432	NM_135785.1	CG6043-RD, transcript variant D (CG6043), mRNA
1.45	7	36	163	429	NM_206357.2	CG33261-RD, transcript variant D (Trl), mRNA
1.45	7	27	117	326	NM_167200.1	CG1343-RB, transcript variant B (Sp1), mRNA
1.45	7	27	117	326	NM_132351.1	CG1343-RA, transcript variant A (Sp1), mRNA
1.45	7	26	126	369	NM_206356.2	CG33261-RB, transcript variant B (Trl), mRNA
1.45	7	8	28	85	NM_001038924.1	CG33261-RG, transcript variant G (Trl), mRNA

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