National Institute of Genetics
Contact us      Quick Search       
NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -
Home Home
Japanese | English  
NIG-RNAi Strains Detail
Request : Click the "Order" button to request for this stock discarded request_help.gif
Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 18321R-3 
 Symbol miple2  Full Name miple2 
 CG No CG18321  Old CG No CG18321 
 Synonyms CG18321, miple2 
 Accession No (Link to NCBI) NM_143739.2 
 Inserted Chr. lll 
 Insertional Mutation  2 semi-lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTGGTTGCGGAAAATGGCGGGG 
 Primer Seq. 3' 
aatctagaggtaccTGTTCGACGACTCTGGCCGTG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 TGGTTGCGGA AAATGGCGGG GCACTGTCAT CACAACTACC ACAACCAAAT CACAAGCAAG 
0061 GCCACAAGAA CACTGATAAG CAACAACGAG GAGGGGGATC CAAGCAGCAG CGAGGACATA 
0121 GCCAACATCA TGGAGGAAAT AGGAAAGGGC CTAAGGCAGC GACTCCAGAG AATGGATCGA 
0181 CTTGCCGATA CGCAAAGAGC GCTTGGTCCA ATTGTGATCA CAAGACCAAC ATGCGTTCAC 
0241 GAGTCCTGTC CCTAAGGAAA GGAGAGCAAA ACTGCTTGCC CACGCGGACC ATACAAAAGA 
0301 AGTGCAAGAA AGGCTGCCGC TATGAAAAGG GTGAATGGTC TCAATGCGTC GGAGGACAAA 
0361 TAACCAGAGA GGATAAGTTG GAACCAGAGG CAACTGGCGG TAGTGATCAA AACTGCAATC 
0421 CTGTGCGTAC CGTCAGCAAG AAGTGTAAAG CAAATGGCAA TTCCAGTGGA GGAAAACAAC 
0481 ACGGCCAGAG TCGTCGAACA  
 in silico PCR Fragment 
0001 TGGTTGCGGA AAATGGCGGG GCACTGTCAT CACAACTACC ACAACCAAAT CACAAGCAAG 
0061 GCCACAAGAA CACTGATAAG CAACAACGAG GAGGGGGATC CAAGCAGCAG CGAGGACATA 
0121 GCCAACATCA TGGAGGAAAT AGGAAAGGGC CTAAGGCAGC GACTCCAGAG AATGGATCGA 
0181 CTTGCCGATA CGCAAAGAGC GCTTGGTCCA ATTGTGATCA CAAGACCAAC ATGCGTTCAC 
0241 GAGTCCTGTC CCTAAGGAAA GGAGAGCAAA ACTGCTTGCC CACGCGGACC ATACAAAAGA 
0301 AGTGCAAGAA AGGCTGCCGC TATGAAAAGG GTGAATGGTC TCAATGCGTC GGAGGACAAA 
0361 TAACCAGAGA GGATAAGTTG GAACCAGAGG CAACTGGCGG TAGTGATCAA AACTGCAATC 
0421 CTGTGCGTAC CGTCAGCAAG AAGTGTAAAG CAAATGGCAA TTCCAGTGGA GGAAAACAAC 
0481 ACGGCCAGAG TCGTCGAACA  
 Assemble Data 
<PRE>

18321R-3.IR_full       1   TGGTTGCGGAAAATGGCGGGGCACTGTCATCACAACTACCACAACCAAATCACAAGCAAG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18321R-3.in silico     1   TGGTTGCGGAAAATGGCGGGGCACTGTCATCACAACTACCACAACCAAATCACAAGCAAG 60


18321R-3.IR_full       61  GCCACAAGAACACTGATAAGCAACAACGAGGAGGGGGATCCAAGCAGCAGCGAGGACATA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18321R-3.in silico     61  GCCACAAGAACACTGATAAGCAACAACGAGGAGGGGGATCCAAGCAGCAGCGAGGACATA 120


18321R-3.IR_full       121 GCCAACATCATGGAGGAAATAGGAAAGGGCCTAAGGCAGCGACTCCAGAGAATGGATCGA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18321R-3.in silico     121 GCCAACATCATGGAGGAAATAGGAAAGGGCCTAAGGCAGCGACTCCAGAGAATGGATCGA 180


18321R-3.IR_full       181 CTTGCCGATACGCAAAGAGCGCTTGGTCCAATTGTGATCACAAGACCAACATGCGTTCAC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18321R-3.in silico     181 CTTGCCGATACGCAAAGAGCGCTTGGTCCAATTGTGATCACAAGACCAACATGCGTTCAC 240


18321R-3.IR_full       241 GAGTCCTGTCCCTAAGGAAAGGAGAGCAAAACTGCTTGCCCACGCGGACCATACAAAAGA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18321R-3.in silico     241 GAGTCCTGTCCCTAAGGAAAGGAGAGCAAAACTGCTTGCCCACGCGGACCATACAAAAGA 300


18321R-3.IR_full       301 AGTGCAAGAAAGGCTGCCGCTATGAAAAGGGTGAATGGTCTCAATGCGTCGGAGGACAAA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18321R-3.in silico     301 AGTGCAAGAAAGGCTGCCGCTATGAAAAGGGTGAATGGTCTCAATGCGTCGGAGGACAAA 360


18321R-3.IR_full       361 TAACCAGAGAGGATAAGTTGGAACCAGAGGCAACTGGCGGTAGTGATCAAAACTGCAATC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18321R-3.in silico     361 TAACCAGAGAGGATAAGTTGGAACCAGAGGCAACTGGCGGTAGTGATCAAAACTGCAATC 420


18321R-3.IR_full       421 CTGTGCGTACCGTCAGCAAGAAGTGTAAAGCAAATGGCAATTCCAGTGGAGGAAAACAAC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18321R-3.in silico     421 CTGTGCGTACCGTCAGCAAGAAGTGTAAAGCAAATGGCAATTCCAGTGGAGGAAAACAAC 480


18321R-3.IR_full       481 ACGGCCAGAGTCGTCGAACA 500
                           ||||||||||||||||||||
18321R-3.in silico     481 ACGGCCAGAGTCGTCGAACA 500

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_143739.2  CG18321-RA (miple2), mRNA 
0   0  2  3  6  NM_132230.2  CG2272-RA (slpr), mRNA 
0   0  2  3  2  NM_206430.1  CG2666-RC, transcript variant C (kkv), mRNA 
0   0  2  3  2  NM_079509.1  CG2666-RA, transcript variant A (kkv), mRNA 
0   0  2  3  2  NM_169052.1  CG2666-RB, transcript variant B (kkv), mRNA 
0   0  2  2  2  NM_169057.1  CG31547-RB, transcript variant B (CG31547), mRNA 
0   0  2  2  2  NM_169058.1  CG31547-RA, transcript variant A (CG31547), mRNA 
0   0  1  3  5  NM_078681.2  CG32540-RA (CCKLR-17D3), mRNA 
0   0  1  3  5  NM_141798.1  CG10703-RA (CG10703), mRNA 
0   0  1  2  3  NM_142692.1  CG5802-RA (CG5802), mRNA 
0   0  1  2  3  NM_142335.2  CG5208-RA (CG5208), mRNA 
0   0  1  2  3  NM_137811.2  CG3264-RA (CG3264), mRNA 
0   0  1  2  2  NM_079565.3  CG16755-RA (Or85f), mRNA 
0   0  1  2  2  NM_168163.1  CG32401-RA (Or65a), mRNA 
0   0  0  21  108  NM_170227.2  CG31439-RA (CG31439), mRNA 
0   0  0  7  4  NM_079289.2  CG10574-RA (I-2), mRNA 
0   0  0  6  2  NM_137634.1  CG13870-RA, transcript variant A (CG13870), mRNA 
0   0  0  6  2  NM_166391.1  CG13870-RB, transcript variant B (CG13870), mRNA 
0   0  0  5  3  NM_140034.2  CG4641-RA (CG4641), mRNA 
0   0  0  4  7  NM_165853.1  CG13188-RA, transcript variant A (CG13188), mRNA 
0   0  0  4  7  NM_136870.1  CG13188-RB, transcript variant B (CG13188), mRNA 
0   0  0  4  3  NM_176537.1  CG33099-RA (CG33099), mRNA 
0   0  0  4  3  NM_133108.2  CG32542-RA (CG32542), mRNA 
0   0  0  4  2  NM_137819.2  CG11206-RA, transcript variant A (CG11206), mRNA 
0   0  0  4  2  NM_206200.1  CG11206-RB, transcript variant B (CG11206), mRNA 
0   0  0  3  12  NM_139488.2  CG1240-RA (CG1240), mRNA 
0   0  0  3  8  NM_141936.1  CG6525-RA (CG6525), mRNA 
0   0  0  3  7  NM_168103.1  CG7507-RB, transcript variant B (Dhc64C), mRNA 
0   0  0  3  7  NM_079205.3  CG7507-RA, transcript variant A (Dhc64C), mRNA 
0   0  0  3  7  NM_080047.2  CG8491-RA (kto), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway

SHIGEN - SHared Information of GENetic resources - National Institute of Genetics (NIG)
 Copyright (c) National Institute of Genetics (NIG)
All rights reserved.
The NIG-Fly is a part of the National BioResource Project which started in 2002.