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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
18321R-1 |
| Symbol |
miple2 |
Full Name |
miple2 |
| CG No |
CG18321 |
Old CG No |
CG18321 |
| Synonyms |
CG18321, miple2 |
| Accession No (Link to NCBI) |
NM_143739.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTGGTTGCGGAAAATGGCGGGG |
| Primer Seq. 3' |
aatctagaggtaccTGTTCGACGACTCTGGCCGTG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TGGTTGCGGA AAATGGCGGG GCACTGTCAT CACAACTACC ACAACCAAAT CACAAGCAAG
0061 GCCACAAGAA CACTGATAAG CAACAACGAG GAGGGGGATC CAAGCAGCAG CGAGGACATA
0121 GCCAACATCA TGGAGGAAAT AGGAAAGGGC CTAAGGCAGC GACTCCAGAG AATGGATCGA
0181 CTTGCCGATA CGCAAAGAGC GCTTGGTCCA ATTGTGATCA CAAGACCAAC ATGCGTTCAC
0241 GAGTCCTGTC CCTAAGGAAA GGAGAGCAAA ACTGCTTGCC CACGCGGACC ATACAAAAGA
0301 AGTGCAAGAA AGGCTGCCGC TATGAAAAGG GTGAATGGTC TCAATGCGTC GGAGGACAAA
0361 TAACCAGAGA GGATAAGTTG GAACCAGAGG CAACTGGCGG TAGTGATCAA AACTGCAATC
0421 CTGTGCGTAC CGTCAGCAAG AAGTGTAAAG CAAATGGCAA TTCCAGTGGA GGAAAACAAC
0481 ACGGCCAGAG TCGTCGAACA |
| in silico PCR Fragment |
0001 TGGTTGCGGA AAATGGCGGG GCACTGTCAT CACAACTACC ACAACCAAAT CACAAGCAAG
0061 GCCACAAGAA CACTGATAAG CAACAACGAG GAGGGGGATC CAAGCAGCAG CGAGGACATA
0121 GCCAACATCA TGGAGGAAAT AGGAAAGGGC CTAAGGCAGC GACTCCAGAG AATGGATCGA
0181 CTTGCCGATA CGCAAAGAGC GCTTGGTCCA ATTGTGATCA CAAGACCAAC ATGCGTTCAC
0241 GAGTCCTGTC CCTAAGGAAA GGAGAGCAAA ACTGCTTGCC CACGCGGACC ATACAAAAGA
0301 AGTGCAAGAA AGGCTGCCGC TATGAAAAGG GTGAATGGTC TCAATGCGTC GGAGGACAAA
0361 TAACCAGAGA GGATAAGTTG GAACCAGAGG CAACTGGCGG TAGTGATCAA AACTGCAATC
0421 CTGTGCGTAC CGTCAGCAAG AAGTGTAAAG CAAATGGCAA TTCCAGTGGA GGAAAACAAC
0481 ACGGCCAGAG TCGTCGAACA |
| Assemble Data |
<PRE>
18321R-1.IR_full 1 TGGTTGCGGAAAATGGCGGGGCACTGTCATCACAACTACCACAACCAAATCACAAGCAAG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18321R-1.in silico 1 TGGTTGCGGAAAATGGCGGGGCACTGTCATCACAACTACCACAACCAAATCACAAGCAAG 60
18321R-1.IR_full 61 GCCACAAGAACACTGATAAGCAACAACGAGGAGGGGGATCCAAGCAGCAGCGAGGACATA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18321R-1.in silico 61 GCCACAAGAACACTGATAAGCAACAACGAGGAGGGGGATCCAAGCAGCAGCGAGGACATA 120
18321R-1.IR_full 121 GCCAACATCATGGAGGAAATAGGAAAGGGCCTAAGGCAGCGACTCCAGAGAATGGATCGA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18321R-1.in silico 121 GCCAACATCATGGAGGAAATAGGAAAGGGCCTAAGGCAGCGACTCCAGAGAATGGATCGA 180
18321R-1.IR_full 181 CTTGCCGATACGCAAAGAGCGCTTGGTCCAATTGTGATCACAAGACCAACATGCGTTCAC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18321R-1.in silico 181 CTTGCCGATACGCAAAGAGCGCTTGGTCCAATTGTGATCACAAGACCAACATGCGTTCAC 240
18321R-1.IR_full 241 GAGTCCTGTCCCTAAGGAAAGGAGAGCAAAACTGCTTGCCCACGCGGACCATACAAAAGA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18321R-1.in silico 241 GAGTCCTGTCCCTAAGGAAAGGAGAGCAAAACTGCTTGCCCACGCGGACCATACAAAAGA 300
18321R-1.IR_full 301 AGTGCAAGAAAGGCTGCCGCTATGAAAAGGGTGAATGGTCTCAATGCGTCGGAGGACAAA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18321R-1.in silico 301 AGTGCAAGAAAGGCTGCCGCTATGAAAAGGGTGAATGGTCTCAATGCGTCGGAGGACAAA 360
18321R-1.IR_full 361 TAACCAGAGAGGATAAGTTGGAACCAGAGGCAACTGGCGGTAGTGATCAAAACTGCAATC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18321R-1.in silico 361 TAACCAGAGAGGATAAGTTGGAACCAGAGGCAACTGGCGGTAGTGATCAAAACTGCAATC 420
18321R-1.IR_full 421 CTGTGCGTACCGTCAGCAAGAAGTGTAAAGCAAATGGCAATTCCAGTGGAGGAAAACAAC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18321R-1.in silico 421 CTGTGCGTACCGTCAGCAAGAAGTGTAAAGCAAATGGCAATTCCAGTGGAGGAAAACAAC 480
18321R-1.IR_full 481 ACGGCCAGAGTCGTCGAACA 500
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18321R-1.in silico 481 ACGGCCAGAGTCGTCGAACA 500
</PRE>
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_143739.2 |
CG18321-RA (miple2), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_132230.2 |
CG2272-RA (slpr), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_206430.1 |
CG2666-RC, transcript variant C (kkv), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_079509.1 |
CG2666-RA, transcript variant A (kkv), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_169052.1 |
CG2666-RB, transcript variant B (kkv), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_169057.1 |
CG31547-RB, transcript variant B (CG31547), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_169058.1 |
CG31547-RA, transcript variant A (CG31547), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_078681.2 |
CG32540-RA (CCKLR-17D3), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_141798.1 |
CG10703-RA (CG10703), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_142692.1 |
CG5802-RA (CG5802), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_142335.2 |
CG5208-RA (CG5208), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_137811.2 |
CG3264-RA (CG3264), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_079565.3 |
CG16755-RA (Or85f), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_168163.1 |
CG32401-RA (Or65a), mRNA |
| 0 |
0 |
0 |
21 |
108 |
NM_170227.2 |
CG31439-RA (CG31439), mRNA |
| 0 |
0 |
0 |
7 |
4 |
NM_079289.2 |
CG10574-RA (I-2), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_137634.1 |
CG13870-RA, transcript variant A (CG13870), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_166391.1 |
CG13870-RB, transcript variant B (CG13870), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_140034.2 |
CG4641-RA (CG4641), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_165853.1 |
CG13188-RA, transcript variant A (CG13188), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_136870.1 |
CG13188-RB, transcript variant B (CG13188), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_176537.1 |
CG33099-RA (CG33099), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_133108.2 |
CG32542-RA (CG32542), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_137819.2 |
CG11206-RA, transcript variant A (CG11206), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_206200.1 |
CG11206-RB, transcript variant B (CG11206), mRNA |
| 0 |
0 |
0 |
3 |
12 |
NM_139488.2 |
CG1240-RA (CG1240), mRNA |
| 0 |
0 |
0 |
3 |
8 |
NM_141936.1 |
CG6525-RA (CG6525), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_168103.1 |
CG7507-RB, transcript variant B (Dhc64C), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_079205.3 |
CG7507-RA, transcript variant A (Dhc64C), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_080047.2 |
CG8491-RA (kto), mRNA |
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| Wing Disc |
GFP
 |
Puc
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Caspase
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DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
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Pathways (updated: 2024/04/26)
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| KEGG Pathway |
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