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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

18296R-3

Symbol

axo

Full Name

axotactin

CG No

CG18296

Old CG No

CG18296

Synonyms

CT1277, CG18296, axo

Accession No (Link to NCBI)

NM_079917.2

Inserted Chr.

lll

Insertional Mutation

lethal

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAAGAGCAACCAGTACGAGAAG

Primer Seq. 3'

aatctagaggtaccCGGGTGCGGAATTGAAATGAA

Predicted Fragment Size

IR fragment full Seq

0001 AAGAGCAACC AGTACGAGAA GTGTGCGGGT CCTGGAGATC CGGGTCCATG CAAGCAGTAC 
0061 ATCTACAAGT GGCGCTATGA GcCAACCACC AACGAGTGCA CCAACTTCAT TTGGGGCGGT 
0121 TGTGAAGGAA ATCCGCAGAA CCGTTTCGGC ACCGAGGCGG AGTGCCTGTT CCACTGCATC 
0181 GGCGGACCAC ACCACTCTGC CACCGTTTCT GCAATCCACC ACCCGTGAGC CAAGCACCAC 
0241 GGAGAGCTCC AGTGCTTCTG GGTCTGCCGT ACACCCAGAG TCCGGCTCAG TCGCCGGACG 
0301 GAATGGGCGG GGCAGAGGGC GGTGATGGCA CAACGCCCGT GCCCATCGAA CAGCGCGGAC 
0361 ACCCGAGCTG ACTTTTCGCG GAGACGGGCC AAGGCAAAAC TTTCATTTTC GCCAAGAACA 
0421 ACACGTTCAT CCAAATGGAC GGCGACATTA TACAGACATT TCAATTAAGA CTTTGCCGTG 
0481 AGATTTCATT TCAATTCCGC ACCCG

in silico PCR Fragment

0001 AAGAGCAACC AGTACGAGAA GTGTGCGGGT CCTGGAGATC CGGGTCCATG CAAGCAGTAC 
0061 ATCTACAAGT GGCGCTATGA GCCAACCACC AACGAGTGCA CCAACTTCAT TTGGGGCGGT 
0121 TGTGAAGGAA ATCCGCAGAA CCGTTTCGGC ACCGAGGCGG AGTGCCTGTT CCACTGCATC 
0181 GGCGGACCAC A-CACTCTGC CACCGTTCCT ACAATCCACC ACCCGTGAGC CAAGCACTAC 
0241 GGAGAGCTCC A-TGCTTCTG GGTCTGCCGT ACACCCAGAG TCCGGCTCAG TCACCGGACG 
0301 GAATGGGCGG GGCAGAGGGC GGTGATGGCA CAACGCCCGT GCCCATCGAA CAGCGCGG-- 
0361 ACCCGAGCTG AC-TTTCGCG GAGACGGGCC AAGGCAAAAC TTTCATTTTC GCCAAGAACA 
0421 ACACGTTCAT CCAAATGGAC GGCGACATTA TACAGACATT TCAATTAAGA CTTTGCCGTG 
0481 AGATTTCATT TCAATTCCGC ACCCG

Assemble Data

<PRE>

18296R-3.IR_full       1   AAGAGCAACCAGTACGAGAAGTGTGCGGGTCCTGGAGATCCGGGTCCATGCAAGCAGTAC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18296R-3.in silico     1   AAGAGCAACCAGTACGAGAAGTGTGCGGGTCCTGGAGATCCGGGTCCATGCAAGCAGTAC 60


18296R-3.IR_full       61  ATCTACAAGTGGCGCTATGAGCCAACCACCAACGAGTGCACCAACTTCATTTGGGGCGGT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18296R-3.in silico     61  ATCTACAAGTGGCGCTATGAGCCAACCACCAACGAGTGCACCAACTTCATTTGGGGCGGT 120


18296R-3.IR_full       121 TGTGAAGGAAATCCGCAGAACCGTTTCGGCACCGAGGCGGAGTGCCTGTTCCACTGCATC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18296R-3.in silico     121 TGTGAAGGAAATCCGCAGAACCGTTTCGGCACCGAGGCGGAGTGCCTGTTCCACTGCATC 180


18296R-3.IR_full       181 GGCGGACCACA<span class="snp"><tt>C</tt></span>CACTCTGCCACCGTT<span class="snp"><tt>T</tt></span>CT<span class="snp"><tt>G</tt></span>CAATCCACCACCCGTGAGCCAAGCAC<span class="snp"><tt>C</tt></span>AC 240
                           |||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||
18296R-3.in silico     181 GGCGGACCACA<span class="snp"><tt>-</tt></span>CACTCTGCCACCGTT<span class="snp"><tt>C</tt></span>CT<span class="snp"><tt>A</tt></span>CAATCCACCACCCGTGAGCCAAGCAC<span class="snp"><tt>T</tt></span>AC 240


18296R-3.IR_full       241 GGAGAGCTCCA<span class="snp"><tt>G</tt></span>TGCTTCTGGGTCTGCCGTACACCCAGAGTCCGGCTCAGTC<span class="snp"><tt>G</tt></span>CCGGACG 300
                           |||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||
18296R-3.in silico     241 GGAGAGCTCCA<span class="snp"><tt>-</tt></span>TGCTTCTGGGTCTGCCGTACACCCAGAGTCCGGCTCAGTC<span class="snp"><tt>A</tt></span>CCGGACG 300


18296R-3.IR_full       301 GAATGGGCGGGGCAGAGGGCGGTGATGGCACAACGCCCGTGCCCATCGAACAGCGCGG<span class="snp"><tt>A</tt></span><span class="snp"><tt>C</tt></span> 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>
18296R-3.in silico     301 GAATGGGCGGGGCAGAGGGCGGTGATGGCACAACGCCCGTGCCCATCGAACAGCGCGG<span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span> 360


18296R-3.IR_full       361 ACCCGAGCTGAC<span class="snp"><tt>T</tt></span>TTTCGCGGAGACGGGCCAAGGCAAAACTTTCATTTTCGCCAAGAACA 420
                           ||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||
18296R-3.in silico     361 ACCCGAGCTGAC<span class="snp"><tt>-</tt></span>TTTCGCGGAGACGGGCCAAGGCAAAACTTTCATTTTCGCCAAGAACA 420


18296R-3.IR_full       421 ACACGTTCATCCAAATGGACGGCGACATTATACAGACATTTCAATTAAGACTTTGCCGTG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18296R-3.in silico     421 ACACGTTCATCCAAATGGACGGCGACATTATACAGACATTTCAATTAAGACTTTGCCGTG 480


18296R-3.IR_full       481 AGATTTCATTTCAATTCCGCACCCG 505
                           |||||||||||||||||||||||||
18296R-3.in silico     481 AGATTTCATTTCAATTCCGCACCCG 505

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	2	NM_079917.2	CG18296-RA (axo), mRNA
0.2	1	5	2	2	NM_140567.2	CG6017-RA (CG6017), mRNA
0	0	4	4	3	NM_169504.1	CG32473-RC, transcript variant C (CG32473), mRNA
0	0	4	4	3	NM_169503.1	CG32473-RA, transcript variant A (CG32473), mRNA
0	0	4	4	3	NM_142019.2	CG32473-RB, transcript variant B (CG32473), mRNA
0	0	2	4	5	NM_136037.2	CG10275-RA (CG10275), mRNA
0	0	2	4	2	NM_168742.2	CG6143-RA, transcript variant A (Pep), mRNA
0	0	2	4	2	NM_206396.1	CG6143-RC, transcript variant C (Pep), mRNA
0	0	2	4	2	NM_080119.3	CG6143-RB, transcript variant B (Pep), mRNA
0	0	2	2	6	NM_136816.1	CG13214-RA, transcript variant A (CG13214), mRNA
0	0	2	2	4	NM_143716.2	CG6754-RB (nbs), mRNA
0	0	1	4	3	NM_134983.2	CG4104-RA (Tps1), mRNA
0	0	1	3	3	NM_135183.3	CG9527-RA (CG9527), mRNA
0	0	1	3	2	NM_137529.2	CG15093-RB, transcript variant B (CG15093), mRNA
0	0	1	3	2	NM_166306.1	CG15093-RA, transcript variant A (CG15093), mRNA
0	0	1	3	2	NM_167580.1	CG32554-RA (CG32554), mRNA
0	0	0	5	2	NM_057546.2	CG3090-RA, transcript variant A (Sox14), mRNA
0	0	0	5	2	NM_134290.1	CG3090-RB, transcript variant B (Sox14), mRNA
0	0	0	4	10	NM_078526.1	CG10759-RA (Or7a), mRNA
0	0	0	4	7	NM_141948.1	CG5333-RA (trus), mRNA
0	0	0	4	7	NM_057805.2	CG9660-RA, transcript variant A (toc), mRNA
0	0	0	4	5	NM_167478.1	CG8933-RB, transcript variant B (exd), mRNA
0	0	0	4	5	NM_167479.1	CG8933-RC, transcript variant C (exd), mRNA
0	0	0	4	5	NM_078636.4	CG8933-RA, transcript variant A (exd), mRNA
0	0	0	4	3	NM_169834.1	CG7720-RA, transcript variant A (CG7720), mRNA
0	0	0	4	3	NM_142496.2	CG7720-RB, transcript variant B (CG7720), mRNA
0	0	0	4	3	NM_167190.1	CG15316-RB, transcript variant B (CG15316), mRNA
0	0	0	4	3	NM_132338.1	CG15316-RA, transcript variant A (CG15316), mRNA
0	0	0	4	3	NM_136256.2	CG8678-RA (CG8678), mRNA
0	0	0	4	2	NM_166705.1	CG2679-RB, transcript variant B (gol), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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