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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

18178R-1

Symbol

CG18178

Full Name

CG18178

CG No

CG18178

Old CG No

CG18178

Synonyms

CG18178

Accession No (Link to NCBI)

NM_140093.1

Inserted Chr.

Insertional Mutation

lethal

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAGCAGGTGGAAAGTGCATCCG

Primer Seq. 3'

aatctagaggtaccTAAGCCCTTGTGCTTGTCATC

Predicted Fragment Size

IR fragment full Seq

0001 AGCAGGTGGA AAGTGCATCC GAGGAGGAGN AAGTGCAGTC CGAAAACTCC GAGGCTTCCG 
0061 ACGAGGaGGA GCTACAGGCA GAGGACTCCG ACGAGGACCA GCTGCAGGCC GNACGACTCC 
0121 GAAGACGAAG ATTCCGATGG ATCCGAACTG GATCTGGACG CCAGTGACAC CGAATCCGAT 
0181 GAAGATGATG GTGAGGAGGA AGATACAATC AAACCGCAGC CCAAAATGCC GTGCCGAGAT 
0241 TATCGACGAG AAGATCCACA CCAAGTACGA GCAGTTAAAG GGCACCCGGC TGTATGTCCG 
0301 GTTTCCCCAA AAACTGCCCC TGGACTTGGA TGAATTCAAT GCCAAGGTGA AGNNTCTGCA 
0361 TCCACTGGTT CTGAAGTCCA CAAAGCCTCG CCAGAAGCAT GCACGTTTCT GCCTCGTCGA 
0421 TTTCAAGTCG AAGGAAGATC GCGATCAAGC CTTTAAGGAT ATCAAGGCTT CGATCGAAAA 
0481 GGATGACAAG CACAAGGGCT TA

in silico PCR Fragment

0001 AGCAGGTGGA AAGTGCATCC GAGGAGGAGG AAGTGCAGTC CGAAAACTCC GAGGCTTCCG 
0061 ACGAGGAGGA GCTACAGGCA GAGGACTCCG ACGAGGACCA GCTGCAGG-C GGACGACTCC 
0121 GAAGACGAAG ATTCCGATGG ATCCGAACTG GATCTGGACG CCAGTGACAC CGAATCCGAT 
0181 GAAGATGATG GTGAGGAGGA AGATACAATC AAACCGCAGC CCAAAA-GCC GTGCCGAGAT 
0241 TATCGACGAG AAGATCCACA CCAAGTACGA GCAGTTAAAG GGCACCCGGC TGTATGTCCG 
0301 GTTTCCCCAA AAACTGCCCC TGGACTTGGA TGAATTCAAT GCCAAGGTGA AGGCTCTGCA 
0361 TCCACTGGTT CTGAAGTCCA CAAAGCCTCG CCAGAAGCAT GCACGTTTCT GCCTCGTCGA 
0421 TTTCAAGTCG AAGGAAGATC GCGATCAAGC CTTTAAGGAT ATCAAGGCTT CGATCGAAAA 
0481 GGATGACAAG CACAAGGGCT TA

Assemble Data

<PRE>

18178R-1.IR_full       1   AGCAGGTGGAAAGTGCATCCGAGGAGGAG<span class="snp"><tt>N</tt></span>AAGTGCAGTCCGAAAACTCCGAGGCTTCCG 60
                           |||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||
18178R-1.in silico     1   AGCAGGTGGAAAGTGCATCCGAGGAGGAG<span class="snp"><tt>G</tt></span>AAGTGCAGTCCGAAAACTCCGAGGCTTCCG 60


18178R-1.IR_full       61  ACGAGGAGGAGCTACAGGCAGAGGACTCCGACGAGGACCAGCTGCAGG<span class="snp"><tt>C</tt></span>CG<span class="snp"><tt>N</tt></span>ACGACTCC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||<span class="snp"><tt>&nbsp;</tt></span>||||||||
18178R-1.in silico     61  ACGAGGAGGAGCTACAGGCAGAGGACTCCGACGAGGACCAGCTGCAGG<span class="snp"><tt>-</tt></span>CG<span class="snp"><tt>G</tt></span>ACGACTCC 120


18178R-1.IR_full       121 GAAGACGAAGATTCCGATGGATCCGAACTGGATCTGGACGCCAGTGACACCGAATCCGAT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18178R-1.in silico     121 GAAGACGAAGATTCCGATGGATCCGAACTGGATCTGGACGCCAGTGACACCGAATCCGAT 180


18178R-1.IR_full       181 GAAGATGATGGTGAGGAGGAAGATACAATCAAACCGCAGCCCAAAA<span class="snp"><tt>T</tt></span>GCCGTGCCGAGAT 240
                           ||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||
18178R-1.in silico     181 GAAGATGATGGTGAGGAGGAAGATACAATCAAACCGCAGCCCAAAA<span class="snp"><tt>-</tt></span>GCCGTGCCGAGAT 240


18178R-1.IR_full       241 TATCGACGAGAAGATCCACACCAAGTACGAGCAGTTAAAGGGCACCCGGCTGTATGTCCG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18178R-1.in silico     241 TATCGACGAGAAGATCCACACCAAGTACGAGCAGTTAAAGGGCACCCGGCTGTATGTCCG 300


18178R-1.IR_full       301 GTTTCCCCAAAAACTGCCCCTGGACTTGGATGAATTCAATGCCAAGGTGAAG<span class="snp"><tt>N</tt></span><span class="snp"><tt>N</tt></span>TCTGCA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>||||||
18178R-1.in silico     301 GTTTCCCCAAAAACTGCCCCTGGACTTGGATGAATTCAATGCCAAGGTGAAG<span class="snp"><tt>G</tt></span><span class="snp"><tt>C</tt></span>TCTGCA 360


18178R-1.IR_full       361 TCCACTGGTTCTGAAGTCCACAAAGCCTCGCCAGAAGCATGCACGTTTCTGCCTCGTCGA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18178R-1.in silico     361 TCCACTGGTTCTGAAGTCCACAAAGCCTCGCCAGAAGCATGCACGTTTCTGCCTCGTCGA 420


18178R-1.IR_full       421 TTTCAAGTCGAAGGAAGATCGCGATCAAGCCTTTAAGGATATCAAGGCTTCGATCGAAAA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18178R-1.in silico     421 TTTCAAGTCGAAGGAAGATCGCGATCAAGCCTTTAAGGATATCAAGGCTTCGATCGAAAA 480


18178R-1.IR_full       481 GGATGACAAGCACAAGGGCTTA 502
                           ||||||||||||||||||||||
18178R-1.in silico     481 GGATGACAAGCACAAGGGCTTA 502

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	24	NM_140093.1	CG18178-RA (CG18178), mRNA
0	0	3	4	4	NM_169221.1	CG7954-RC, transcript variant C (stck), mRNA
0	0	3	4	4	NM_169220.1	CG7954-RB, transcript variant B (stck), mRNA
0	0	3	4	4	NM_079554.2	CG7954-RA, transcript variant A (stck), mRNA
0	0	3	2	2	NM_080142.2	CG1925-RA (mus205), mRNA
0	0	2	7	6	NM_140901.2	CG7762-RA (Rpn1), mRNA
0	0	2	2	7	NM_165156.1	CG5813-RB, transcript variant B (chif), mRNA
0	0	2	2	7	NM_078859.2	CG5813-RA, transcript variant A (chif), mRNA
0	0	1	7	15	NM_131954.1	CG7024-RA (CG7024), mRNA
0	0	1	4	4	NM_166734.1	CG32006-RA (CG32006), mRNA
0	0	1	3	10	NM_080250.2	CG6224-RA (dbo), mRNA
0	0	1	3	9	NM_165243.1	CG15154-RA, transcript variant A (Socs36E), mRNA
0	0	1	3	9	NM_078869.4	CG15154-RB, transcript variant B (Socs36E), mRNA
0	0	1	3	7	NM_135461.2	CG4364-RA (CG4364), mRNA
0	0	1	3	5	NM_164955.1	CG6141-RB, transcript variant B (RpL9), mRNA
0	0	1	3	5	NM_057813.3	CG6141-RA, transcript variant A (RpL9), mRNA
0	0	1	3	3	NM_137538.1	CG15100-RA (CG15100), mRNA
0	0	1	3	3	NM_145192.2	CG15107-RA (CG15107), mRNA
0	0	1	3	2	NM_135183.3	CG9527-RA (CG9527), mRNA
0	0	1	3	2	NM_136289.3	CG3651-RA (CG3651), mRNA
0	0	1	2	4	NM_167027.2	CG6824-RC, transcript variant C (ovo), mRNA
0	0	1	2	4	NM_167026.2	CG6824-RB, transcript variant B (ovo), mRNA
0	0	1	2	4	NM_001038742.1	CG6824-RD, transcript variant D (ovo), mRNA
0	0	1	2	4	NM_080338.3	CG6824-RA, transcript variant A (ovo), mRNA
0	0	1	2	3	NM_058131.3	CG6712-RA (CG6712), mRNA
0	0	1	2	2	NM_142806.1	CG5278-RA (CG5278), mRNA
0	0	1	2	2	NM_167228.1	CG32681-RA (CG32681), mRNA
0	0	0	8	37	NM_168401.1	CG6611-RC, transcript variant C (ect), mRNA
0	0	0	8	26	NM_168400.1	CG6611-RB, transcript variant B (ect), mRNA
0	0	0	8	26	NM_079967.4	CG6611-RA, transcript variant A (ect), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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