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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 18128R-2 
 Symbol CG18128  Full Name CG18128 
 CG No CG18128  Old CG No CG18128 
 Synonyms CG18128 
 Accession No (Link to NCBI) NM_137978.1 
 Inserted Chr. ll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgGTGCAACTACGACTGCTGCAA 
 Primer Seq. 3'
aatctagaggtaccTTATGTCGCCCAGGGCGAATC 
 Predicted Fragment Size
500 
 IR fragment full Seq
0001 GTGCAACTAC GACTGCTGCA AGTCCAGGTC GCCGATTGCC AAGCGAATGG CGGCCAGGAA 
0061 GCTTCTGCAG ATGGAGGAGG AGGAACGCCG GNAAGCCCAA GCTGGTCATC CCGACGCCCC 
0121 AGAGCCTCTT CTACCCCTTC GAGGAAGTGG AGGCCATGGC CAAGNTACAT CGTGAACGTT 
0181 AGCCACATCC GGCCAAAGTA CGGACTAATC TGCGGCAGCT TCCTGAGCGA TATGGTCAGC 
0241 TTGGTGGAGC AGCCGGTGGT CATTCCCTAC GAGGACATAC CCAACTTCCC CGACGGCATA 
0301 GAGCCGGACT GCAGCTTCGT CTNTGGGCAC GGTCATGGGA GCGCCCATAA TAGCGCTGGT 
0361 CCACAGTTTC CACTCCTGCG ACGGCTACAA CCTGGCCACC TGTNCGCTGC CCGTCCGCGT 
0421 GATGCAGTTG TGCGGGGTCA GGACCATCAT GCTGACCTCT GAAGCAGCGG CCGTAGATCA 
0481 CGGANTcNCC CTGGGCGACA TAA 
 in silico PCR Fragment
0001 GTGCAACTAC GACTGCTGCA AGTCCAGGTC GCCGATTGCC AAGCGAATGG CGGCCAGGAA 
0061 GCTTCTGCAG ATGGAGGAGG AGGAACGCCG G-AAGCCCAA GCTGGTCATC CCGACGCCCC 
0121 AGAGCCTCTT CTACCCCTTC GAGGAAGTCG AGGCCATGGC CAAG-TACAT CGTGAACGTT 
0181 AGCCACATCA GGCCAAAGTA CGGACTAATC TGCGGCAGCT TCCTGAGCGA TATGGTCAGC 
0241 TTGGTGGAGC AGCCGGTGGT CATTCCCTAC GAGGACATAC CCAACTTCCC CGACGGCATA 
0301 GAGCCGGACT GCAGCTTCGT CT-TGGGCAC GGTCATGGGA GCGCCCATAA TAGCGCTGGT 
0361 CCACAGTTTC CACTCCTGCG ACGGCTACAA CCTGGCCACC TGTGCGCTGC CCGTCCGCGT 
0421 GATGCAGTTG TGCGGGGTCA GGACCATCAT GCTGACCTCT GAAGCAGCGG CCGTAGATCA 
0481 CGGATTCGCC CTGGGCGACA TAA 
 Assemble Data

18128R-2.IR_full       1   GTGCAACTACGACTGCTGCAAGTCCAGGTCGCCGATTGCCAAGCGAATGGCGGCCAGGAA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18128R-2.in silico     1   GTGCAACTACGACTGCTGCAAGTCCAGGTCGCCGATTGCCAAGCGAATGGCGGCCAGGAA 60


18128R-2.IR_full       61  GCTTCTGCAGATGGAGGAGGAGGAACGCCGGNAAGCCCAAGCTGGTCATCCCGACGCCCC 120
                           ||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||
18128R-2.in silico     61  GCTTCTGCAGATGGAGGAGGAGGAACGCCGG-AAGCCCAAGCTGGTCATCCCGACGCCCC 120


18128R-2.IR_full       121 AGAGCCTCTTCTACCCCTTCGAGGAAGTGGAGGCCATGGCCAAGNTACATCGTGAACGTT 180
                           |||||||||||||||||||||||||||| ||||||||||||||| |||||||||||||||
18128R-2.in silico     121 AGAGCCTCTTCTACCCCTTCGAGGAAGTCGAGGCCATGGCCAAG-TACATCGTGAACGTT 180


18128R-2.IR_full       181 AGCCACATCCGGCCAAAGTACGGACTAATCTGCGGCAGCTTCCTGAGCGATATGGTCAGC 240
                           ||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||
18128R-2.in silico     181 AGCCACATCAGGCCAAAGTACGGACTAATCTGCGGCAGCTTCCTGAGCGATATGGTCAGC 240


18128R-2.IR_full       241 TTGGTGGAGCAGCCGGTGGTCATTCCCTACGAGGACATACCCAACTTCCCCGACGGCATA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18128R-2.in silico     241 TTGGTGGAGCAGCCGGTGGTCATTCCCTACGAGGACATACCCAACTTCCCCGACGGCATA 300


18128R-2.IR_full       301 GAGCCGGACTGCAGCTTCGTCTNTGGGCACGGTCATGGGAGCGCCCATAATAGCGCTGGT 360
                           |||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||
18128R-2.in silico     301 GAGCCGGACTGCAGCTTCGTCT-TGGGCACGGTCATGGGAGCGCCCATAATAGCGCTGGT 360


18128R-2.IR_full       361 CCACAGTTTCCACTCCTGCGACGGCTACAACCTGGCCACCTGTNCGCTGCCCGTCCGCGT 420
                           ||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||
18128R-2.in silico     361 CCACAGTTTCCACTCCTGCGACGGCTACAACCTGGCCACCTGTGCGCTGCCCGTCCGCGT 420


18128R-2.IR_full       421 GATGCAGTTGTGCGGGGTCAGGACCATCATGCTGACCTCTGAAGCAGCGGCCGTAGATCA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18128R-2.in silico     421 GATGCAGTTGTGCGGGGTCAGGACCATCATGCTGACCTCTGAAGCAGCGGCCGTAGATCA 480


18128R-2.IR_full       481 CGGANTCNCCCTGGGCGACATAA 503
                           |||| || |||||||||||||||
18128R-2.in silico     481 CGGATTCGCCCTGGGCGACATAA 503

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  NM_137978.1  CG18128-RA (CG18128), mRNA 
0   NM_206262.1  CG12734-RB, transcript variant B (CG12734), mRNA 
0   NM_139523.1  CG12734-RA, transcript variant A (CG12734), mRNA 
0   NM_140225.3  CG6091-RA, transcript variant A (CG6091), mRNA 
0   NM_168465.2  CG6091-RC, transcript variant C (CG6091), mRNA 
0   NM_140301.1  CG11529-RA (CG11529), mRNA 
0   NM_143573.1  CG15545-RA (CG15545), mRNA 
0   12  NM_170038.1  CG31151-RB, transcript variant B (wge), mRNA 
0   12  NM_170037.1  CG31151-RA, transcript variant A (wge), mRNA 
0   12  NM_001043281.1  CG31151-RC, transcript variant C (wge), mRNA 
0   NM_137119.2  CG10105-RA (CG10105), mRNA 
0   NM_001043208.1  CG41467-RA, transcript variant A (CG41467), mRNA 
0   NM_144196.1  CG8620-RA (CG8620), mRNA 
0   NM_078545.2  CG12653-RA (btd), mRNA 
0   NM_136902.2  CG8858-RA (CG8858), mRNA 
0   NM_139462.1  CG13800-RA (CG13800), mRNA 
0   NM_141574.2  CG13318-RA (CG13318), mRNA 
0   NM_176508.1  CG3962-RC, transcript variant C (Keap1), mRNA 
0   NM_142337.1  CG3962-RA, transcript variant A (Keap1), mRNA 
0   NM_169744.1  CG3962-RB, transcript variant B (Keap1), mRNA 
0   NM_176472.1  CG3359-RK, transcript variant K (mfas), mRNA 
0   NM_176470.1  CG3359-RP, transcript variant P (mfas), mRNA 
0   NM_176468.1  CG3359-RD, transcript variant D (mfas), mRNA 
0   NM_176475.1  CG3359-RF, transcript variant F (mfas), mRNA 
0   NM_176469.1  CG3359-RL, transcript variant L (mfas), mRNA 
0   NM_079600.2  CG3359-RC, transcript variant C (mfas), mRNA 
0   NM_176474.1  CG3359-RO, transcript variant O (mfas), mRNA 
0   NM_176476.1  CG3359-RG, transcript variant G (mfas), mRNA 
0   NM_176478.1  CG3359-RJ, transcript variant J (mfas), mRNA 
0   NM_176467.1  CG3359-RN, transcript variant N (mfas), mRNA 
 Wing Disc
GFP
Puc
Caspase
DAPI
Merge
Figure
JNK signal
N
 
DB
DC
++ 
DM
 
VM
 
VB
 
Caspase-3 signal
N
DB
DC
++ 
DM
VM
VB
 
Dcompartment   Penetrance
   

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