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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

18063R-3

Symbol

CG18063

Full Name

CG18063

CG No

CG18063

Old CG No

CG18063

Synonyms

DS07486.5, BG:DS07486.5, CG18063

Accession No (Link to NCBI)

NM_135931.1

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGTTCAAAGCGGCTCGCACAAT

Primer Seq. 3'

aatctagaggtaccATGTGGCCGTAAACGTCTTTC

Predicted Fragment Size

IR fragment full Seq

0001 GTTCAAAGCG GCTCGCACAA TTCAGCGTTA TATTCACGGA TGGCTAGTTC GAGAAAATTT 
0061 CCGAAAACTT CAGAAGTCTG CGATTATTAT TCAAAAATGG TGGCGCCGTT TTGAGGCTCA 
0121 GAAAAATTTG CTTTATGTGG CCGAAAGCGC TTTGCAGTCG GCTGTTTTGG CCCATTACGA 
0181 CAAGTCTGCT ACCCTGATTC AGACGCTCTA TCGCGGATGG TGGTCGCGAA AACATATCTT 
0241 TGACCTAACC ATGCTCAAGA GCGTGCAGAT AATGTTGGCA AAGGACCTGA TTCACTCTTT 
0301 GGTCAATTAC TTGCACGCAA CGAAAAATTC TGAAATGCTT CCAGGCATTT ACACGATACG 
0361 GGACTCGAGC ATATGTCTGG AAACATTGGA GGAACTGATG GCAACATTTG GCTTTCGATT 
0421 CTATAACGCT AATGCTTGCT ACAAAATGAA GGAAACGTTG TCAATGGTGG CACAGAGCCG 
0481 AAAGACGTTT ACGGCCACAT

in silico PCR Fragment

0001 GTTCAAAGCG GCTCGCACAA TTCAGCGTTA TATTCACGGA TGGCTAGTTC GAGAAAATTT 
0061 CCGAAAACTT CAGAAGTCTG CGATTATTAT TCAAAAATGG TGGCGCCGTT TTGAGGCTCA 
0121 GAAAAATTTG CTTTATGTGG CCGAAAGCGC TTTGCAGTCG GCTGTTTTGG CCCATTACGA 
0181 CAAGTCTGCT ACCCTGATTC AGACGCTCTA TCGCGGATGG TGGTCGCGAA AACATATCTT 
0241 TGACCTAACC ATGCTCAAGA GCGTGCAGAT AATGTTGGCA AAGGACCTGA TTCACTCTTT 
0301 GGTCAATTAC TTGCACGCAA CGAAAAATTC TGAAATGCTT CCAGGCATTT ACACGATACG 
0361 GGACTCGAGC ATATGTCTGG AAACATTGGA GGAACTGATG GCAACATTTG GCTTTCGATT 
0421 CTATAACGCT AATGCTTGCT ACAAAATGAA GGAAACGTTG TCAATGGTGG CACAGAGCCG 
0481 AAAGACGTTT ACGGCCACAT

Assemble Data


18063R-3.IR_full       1   GTTCAAAGCGGCTCGCACAATTCAGCGTTATATTCACGGATGGCTAGTTCGAGAAAATTT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18063R-3.in silico     1   GTTCAAAGCGGCTCGCACAATTCAGCGTTATATTCACGGATGGCTAGTTCGAGAAAATTT 60


18063R-3.IR_full       61  CCGAAAACTTCAGAAGTCTGCGATTATTATTCAAAAATGGTGGCGCCGTTTTGAGGCTCA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18063R-3.in silico     61  CCGAAAACTTCAGAAGTCTGCGATTATTATTCAAAAATGGTGGCGCCGTTTTGAGGCTCA 120


18063R-3.IR_full       121 GAAAAATTTGCTTTATGTGGCCGAAAGCGCTTTGCAGTCGGCTGTTTTGGCCCATTACGA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18063R-3.in silico     121 GAAAAATTTGCTTTATGTGGCCGAAAGCGCTTTGCAGTCGGCTGTTTTGGCCCATTACGA 180


18063R-3.IR_full       181 CAAGTCTGCTACCCTGATTCAGACGCTCTATCGCGGATGGTGGTCGCGAAAACATATCTT 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18063R-3.in silico     181 CAAGTCTGCTACCCTGATTCAGACGCTCTATCGCGGATGGTGGTCGCGAAAACATATCTT 240


18063R-3.IR_full       241 TGACCTAACCATGCTCAAGAGCGTGCAGATAATGTTGGCAAAGGACCTGATTCACTCTTT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18063R-3.in silico     241 TGACCTAACCATGCTCAAGAGCGTGCAGATAATGTTGGCAAAGGACCTGATTCACTCTTT 300


18063R-3.IR_full       301 GGTCAATTACTTGCACGCAACGAAAAATTCTGAAATGCTTCCAGGCATTTACACGATACG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18063R-3.in silico     301 GGTCAATTACTTGCACGCAACGAAAAATTCTGAAATGCTTCCAGGCATTTACACGATACG 360


18063R-3.IR_full       361 GGACTCGAGCATATGTCTGGAAACATTGGAGGAACTGATGGCAACATTTGGCTTTCGATT 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18063R-3.in silico     361 GGACTCGAGCATATGTCTGGAAACATTGGAGGAACTGATGGCAACATTTGGCTTTCGATT 420


18063R-3.IR_full       421 CTATAACGCTAATGCTTGCTACAAAATGAAGGAAACGTTGTCAATGGTGGCACAGAGCCG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18063R-3.in silico     421 CTATAACGCTAATGCTTGCTACAAAATGAAGGAAACGTTGTCAATGGTGGCACAGAGCCG 480


18063R-3.IR_full       481 AAAGACGTTTACGGCCACAT 500
                           ||||||||||||||||||||
18063R-3.in silico     481 AAAGACGTTTACGGCCACAT 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_135931.1	CG18063-RA, transcript variant A (CG18063), mRNA
100	482	0	0	0	NM_165141.1	CG18063-RB, transcript variant B (CG18063), mRNA
0	0	2	2	6	NM_079224.3	CG10037-RA (vvl), mRNA
0	0	2	2	3	NM_136937.2	CG8525-RA (CG8525), mRNA
0	0	1	5	4	NM_134967.2	CG3980-RB, transcript variant B (CG3980), mRNA
0	0	1	5	4	NM_164563.2	CG3980-RA, transcript variant A (CG3980), mRNA
0	0	1	3	2	NM_135168.3	CG9491-RA (Gef26), mRNA
0	0	1	2	4	NM_134507.1	CG14234-RA (CG14234), mRNA
0	0	0	4	3	NM_079494.3	CG8779-RA, transcript variant A (nrm), mRNA
0	0	0	4	3	NM_001043156.1	CG8779-RB, transcript variant B (nrm), mRNA
0	0	0	4	2	NM_176592.1	CG12071-RB, transcript variant B (CG12071), mRNA
0	0	0	4	2	NM_143432.2	CG31445-RA (CG31445), mRNA
0	0	0	4	2	NM_143575.2	CG12071-RA, transcript variant A (CG12071), mRNA
0	0	0	3	7	NM_001042904.1	CG15148-RB, transcript variant B (btv), mRNA
0	0	0	3	6	NM_176464.1	CG31211-RB, transcript variant B (CG31211), mRNA
0	0	0	3	6	NM_141874.2	CG31211-RA, transcript variant A (CG31211), mRNA
0	0	0	3	6	NM_176465.1	CG31211-RC, transcript variant C (CG31211), mRNA
0	0	0	3	6	NM_057674.2	CG11482-RA (Mlh1), mRNA
0	0	0	3	6	NM_079764.2	CG6875-RA (asp), mRNA
0	0	0	3	6	NM_136013.1	CG15148-RA, transcript variant A (btv), mRNA
0	0	0	3	6	NM_001042905.1	CG15148-RC, transcript variant C (btv), mRNA
0	0	0	3	5	NM_167251.1	CG1691-RF, transcript variant F (Imp), mRNA
0	0	0	3	3	NM_165130.1	CG3903-RB, transcript variant B (Gli), mRNA
0	0	0	3	3	NM_057254.3	CG3903-RA, transcript variant A (Gli), mRNA
0	0	0	3	3	NM_165128.1	CG3903-RD, transcript variant D (Gli), mRNA
0	0	0	3	3	NM_165127.1	CG3903-RC, transcript variant C (Gli), mRNA
0	0	0	3	3	NM_057768.2	CG7779-RA (Cng), mRNA
0	0	0	3	3	NM_057471.4	CG4260-RA, transcript variant A (alpha-Adaptin), mRNA
0	0	0	3	3	NM_165129.1	CG3903-RE, transcript variant E (Gli), mRNA
0	0	0	3	3	NM_166591.1	CG30416-RA (CG30416), mRNA

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