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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
17998R-3 |
| Symbol |
Gprk2 |
Full Name |
G protein-coupled receptor kinase 2 |
| CG No |
CG17998 |
Old CG No |
CG17998 |
| Synonyms |
154978_at, gprk2, CG17998, Gprk-2, Gprk2 |
| Accession No (Link to NCBI) |
NM_057519.3 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Cho Y, Lai CM, Lin KY, Hsu HJ.<br> A Targeted <i>RNAi</i> Screen Reveals <i>Drosophila</i> Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.<br> G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.<br> An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.<br> PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Valanne S, Myllymäki H, Kallio J, Schmid MR, Kleino A, Murumägi A, Airaksinen L, Kotipelto T, Kaustio M, Ulvila J, Esfahani SS, Engström Y, Silvennoinen O, Hultmark D, Parikka M, Rämet M.<br> Genome-wide RNA interference in Drosophila cells identifies G protein-coupled receptor kinase 2 as a conserved regulator of NF-kappaB signaling.<br> J Immunol (2010) 184(11) 6188-98
[
PubMed ID = 20421637
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTGGCCAATACGGTCTACTTGA |
| Primer Seq. 3' |
aatctagaggtaccGTGCTGCGAGTTGTTGCTGTT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TGGCCAATAC GGTCTACTTG AAAGCGAGAG AAGGTGGTTC CGACAGCAAC AAGGGAAAGA
0061 GNCAAAAAAT GGCGCAAAAT ATTGCAATTC CCACACATAT CGCAATGCAT CAACCTGAAA
0121 GACAAATTAG ATATAAGCTA TGGCTACGTG ATAGATCAGC AACCCATTGG TCGTGAGCTC
0181 TTCCGTCTGT TTTGCGAGAA CAAACGGCCC GTCTACTTTC GCTACATCAC CTTCCTGGAT
0241 GAGGTGGTCA AATACGAGAT CGAGTACATC TCGAACCGCA TATTCATTGG CCATGACATC
0301 GGCCGTCGCT TCTTGGATGT CGAGGCACAG CTGGAGCTGC GCAACGGCAG CGGGGGCGAC
0361 GCCTTGGACG CCGAGACGCA GGAGGAGCTG CTGCTCAACA GCAGCAATGC CAATCCAACT
0421 GAAACAGCTG AGACTGAACA CTGCAACAAT ACCACCGCCA ACAACTGCAA CAACATCAAC
0481 AACAGCAACA ACTCGCATGC Ac |
| in silico PCR Fragment |
0001 TGGCCAATAC GGTCTACTTG AAAGCGAGAG AAGGTGGTTC CGACAGCAAC AAGGGAAAGA
0061 G-CAAAAAAT GGCGCAAAAT ATTGCAATTC CCACACATAT CGCAATGCAT CAACCTGAAA
0121 GACAAATTAG ATATAAGCTA TGGCTACGTG ATAGATCAGC AACCCATTGG TCGTGAGCTC
0181 TTCCGTCTGT TTTGCGAGAA CAAACGGCCC GTCTACTTTC GCTACATCAC CTTCCTGGAT
0241 GAGGTGGTCA AATACGAGAT CGAGTACATC TCGAACCGCA TATTCATTGG CCATGACATC
0301 GGCCGTCGCT TCTTGGATGT CGAGGCACAG CTGGAGCTGC GCAACGGCAG CGGGGGCGAC
0361 GCCTTGGACG CCGAGACGCA GGAGGAGCTG CTGCTCAACA GCAGCAATGC CAATCCAACT
0421 GAAACAGCTG AGACTGAACA CTGCAACAAT ACCACCGCCA ACAACTGCAA CAACATCAAC
0481 AACAGCAACA ACTCGCA-GC AC |
| Assemble Data |
<PRE>
17998R-3.IR_full 1 TGGCCAATACGGTCTACTTGAAAGCGAGAGAAGGTGGTTCCGACAGCAACAAGGGAAAGA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17998R-3.in silico 1 TGGCCAATACGGTCTACTTGAAAGCGAGAGAAGGTGGTTCCGACAGCAACAAGGGAAAGA 60
17998R-3.IR_full 61 G<span class="snp"><tt>N</tt></span>CAAAAAATGGCGCAAAATATTGCAATTCCCACACATATCGCAATGCATCAACCTGAAA 120
|<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17998R-3.in silico 61 G<span class="snp"><tt>-</tt></span>CAAAAAATGGCGCAAAATATTGCAATTCCCACACATATCGCAATGCATCAACCTGAAA 120
17998R-3.IR_full 121 GACAAATTAGATATAAGCTATGGCTACGTGATAGATCAGCAACCCATTGGTCGTGAGCTC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17998R-3.in silico 121 GACAAATTAGATATAAGCTATGGCTACGTGATAGATCAGCAACCCATTGGTCGTGAGCTC 180
17998R-3.IR_full 181 TTCCGTCTGTTTTGCGAGAACAAACGGCCCGTCTACTTTCGCTACATCACCTTCCTGGAT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17998R-3.in silico 181 TTCCGTCTGTTTTGCGAGAACAAACGGCCCGTCTACTTTCGCTACATCACCTTCCTGGAT 240
17998R-3.IR_full 241 GAGGTGGTCAAATACGAGATCGAGTACATCTCGAACCGCATATTCATTGGCCATGACATC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17998R-3.in silico 241 GAGGTGGTCAAATACGAGATCGAGTACATCTCGAACCGCATATTCATTGGCCATGACATC 300
17998R-3.IR_full 301 GGCCGTCGCTTCTTGGATGTCGAGGCACAGCTGGAGCTGCGCAACGGCAGCGGGGGCGAC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17998R-3.in silico 301 GGCCGTCGCTTCTTGGATGTCGAGGCACAGCTGGAGCTGCGCAACGGCAGCGGGGGCGAC 360
17998R-3.IR_full 361 GCCTTGGACGCCGAGACGCAGGAGGAGCTGCTGCTCAACAGCAGCAATGCCAATCCAACT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17998R-3.in silico 361 GCCTTGGACGCCGAGACGCAGGAGGAGCTGCTGCTCAACAGCAGCAATGCCAATCCAACT 420
17998R-3.IR_full 421 GAAACAGCTGAGACTGAACACTGCAACAATACCACCGCCAACAACTGCAACAACATCAAC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17998R-3.in silico 421 GAAACAGCTGAGACTGAACACTGCAACAATACCACCGCCAACAACTGCAACAACATCAAC 480
17998R-3.IR_full 481 AACAGCAACAACTCGCA<span class="snp"><tt>T</tt></span>GCAC 502
|||||||||||||||||<span class="snp"><tt> </tt></span>||||
17998R-3.in silico 481 AACAGCAACAACTCGCA<span class="snp"><tt>-</tt></span>GCAC 502
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
17 |
NM_057519.3 |
CG17998-RA (Gprk2), mRNA |
| 1.03 |
5 |
9 |
45 |
156 |
NM_135615.2 |
CG6700-RA (CG6700), mRNA |
| 0.41 |
2 |
3 |
15 |
84 |
NM_079736.2 |
CG10868-RA, transcript variant A (orb), mRNA |
| 0.41 |
2 |
3 |
13 |
74 |
NM_170062.1 |
CG10868-RB, transcript variant B (orb), mRNA |
| 0.41 |
2 |
3 |
13 |
74 |
NM_170063.2 |
CG10868-RD, transcript variant D (orb), mRNA |
| 0.41 |
2 |
3 |
8 |
58 |
NM_170064.1 |
CG10868-RC, transcript variant C (orb), mRNA |
| 0.41 |
2 |
3 |
8 |
58 |
NM_170065.2 |
CG10868-RE, transcript variant E (orb), mRNA |
| 0.2 |
1 |
4 |
17 |
36 |
NM_001038966.1 |
CG33967-RA (CG33967), mRNA |
| 0.2 |
1 |
2 |
17 |
71 |
NM_079015.2 |
CG8404-RA (Sox15), mRNA |
| 0.2 |
1 |
2 |
2 |
2 |
NM_057581.4 |
CG18466-RB, transcript variant B (Nmdmc), mRNA |
| 0.2 |
1 |
2 |
2 |
2 |
NM_057582.4 |
CG18466-RA, transcript variant A (Nmdmc), mRNA |
| 0 |
0 |
12 |
39 |
107 |
NM_166987.2 |
CG32781-RB (CG32781), mRNA |
| 0 |
0 |
10 |
45 |
195 |
NM_135077.2 |
CG14023-RA (CG14023), mRNA |
| 0 |
0 |
10 |
27 |
111 |
NM_079507.2 |
CG2530-RA (corto), mRNA |
| 0 |
0 |
9 |
19 |
24 |
NM_078692.2 |
CG11937-RA (amn), mRNA |
| 0 |
0 |
9 |
14 |
50 |
NM_164430.2 |
CG31666-RD, transcript variant D (CG31666), mRNA |
| 0 |
0 |
8 |
68 |
220 |
NM_078592.2 |
CG11172-RA (NFAT), mRNA |
| 0 |
0 |
8 |
30 |
88 |
NM_142521.1 |
CG6026-RA (CG6026), mRNA |
| 0 |
0 |
7 |
62 |
422 |
NM_167335.1 |
CG4013-RC, transcript variant C (Smr), mRNA |
| 0 |
0 |
7 |
62 |
421 |
NM_167334.1 |
CG4013-RB, transcript variant B (Smr), mRNA |
| 0 |
0 |
7 |
62 |
421 |
NM_080536.2 |
CG4013-RA, transcript variant A (Smr), mRNA |
| 0 |
0 |
7 |
30 |
103 |
NM_176184.2 |
CG18250-RC, transcript variant C (Dg), mRNA |
| 0 |
0 |
7 |
25 |
133 |
NM_206357.2 |
CG33261-RD, transcript variant D (Trl), mRNA |
| 0 |
0 |
7 |
25 |
130 |
NM_206360.1 |
CG33261-RF, transcript variant F (Trl), mRNA |
| 0 |
0 |
7 |
25 |
130 |
NM_206358.2 |
CG33261-RA, transcript variant A (Trl), mRNA |
| 0 |
0 |
7 |
14 |
74 |
NM_078601.2 |
CG9533-RA (rut), mRNA |
| 0 |
0 |
7 |
11 |
21 |
NM_079128.2 |
CG4859-RB, transcript variant B (Mmp1), mRNA |
| 0 |
0 |
7 |
11 |
21 |
NM_166683.1 |
CG4859-RA, transcript variant A (Mmp1), mRNA |
| 0 |
0 |
6 |
146 |
376 |
NM_168571.2 |
CG32133-RA (CG32133), mRNA |
| 0 |
0 |
6 |
17 |
111 |
NM_167387.1 |
CG32611-RB (CG32611), mRNA |
|
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Pathways (updated: 2024/04/26)
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| KEGG Pathway |
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