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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
17894R-3 |
| Symbol |
cnc |
Full Name |
cap-n-collar |
| CG No |
CG17894 |
Old CG No |
CG17894 |
| Synonyms |
CG4578, Cnc, CNC, l(3)j5E7, CNC_DROME, 5134, l(3)03921, CG4566, CG17894, anon-WO0153538.7, anon-WO0153538.6, cnc |
| Accession No (Link to NCBI) |
NM_170055.1 |
| Inserted Chr. |
X |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Cho Y, Lai CM, Lin KY, Hsu HJ.<br> A Targeted <i>RNAi</i> Screen Reveals <i>Drosophila</i> Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.<br> G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.<br> DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.<br> Dev Biol (2017) 421(2) 233-244
[
PubMed ID = 27908785
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGCAGTGGACATGTTAGCAACG |
| Primer Seq. 3' |
aatctagaggtaccCTTGATACTCTGCGATTGGAC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GCAGTGGACA TGTTAGCAAC GGCTCCGGTA GCTCGGCACA ACTTGGGGCG GGAAATCCgC
0061 ACGGTAACCA GGCCAACgGA GCGTCCGGCG GCTGTTGGCT cAATGAGCGG ATCAGCTGTG
0121 GGCGCTGGAN CTACGGGAAT GACCGCCGAT CTCTTGGCAA GCGGCgGTGC AGGAGCACAA
0181 GGCGGTGCGG ATCGCTTGGA CGCGTCCAgc GACAGTGCTG TCAGTTCGAT GGGCTCCCGA
0241 GCGATGTGCC GTCCCTCTCC GACGGCGAGT GGGGTGAGGG CATGTGATCT CCGCCCAGGA
0301 TTACCATCAG GGCAAGTACG GAGGCCCCTA CGACTTCAGC TACAACAATA ATTCACGGCT
0361 TAGCACCGCC ACACGTCAGC CGCCGGTGGC GCAGAAGAAG CATCAGCTGT ACGGCAAGAG
0421 GGATCCCCAT AAGCATGACA GCCATCGGCT TTGCCGACCA ACAGCTCCAC CAGCAGCCGC
0481 GACTGCAGTC CAATCGCAGA GTATCAAAGG TG |
| in silico PCR Fragment |
0001 GCAGTGGACA TGTTAGCAAC GGCTCCGGTA GCTCGGCACA ACTTGGGGCG GGAAATCCGC
0061 ACGGTAACCA GGCCAACGGA GCGTCCGGCG GC-GTTGGCT CAATGAGCGG ATCAGCTGTG
0121 GGCGCTGGAG CTACGGGAAT GACCGCCGAT CTCTTGGCAA GCGGCGGTGC AGGAGCACAA
0181 GGCGGTGCGG ATCGCTTGGA CGCGTCCAGC GACAGTGCTG TCAGTTCGAT GGGCT-CCGA
0241 GCGA-GTGCC GTCCCTCTCC GACGGCGAGT GGGGTGAGGG CA-GTGA-CT CCGCCCAGGA
0301 TTACCATCAG GGCAAGTACG GAGGCCCCTA CGACTTCAGC TACAACAATA ATTCACGGCT
0361 TAGCACCGCC ACACGTCAGC CGCCGGTGGC GCAGAAGAAG CATCAGCTGT ACGGCAAGAG
0421 GGATCCCCAT AAGCA-GAC- GCCATCGGCT TTGCC-ACCA ACAGCTCCAC CAGCAGCCGC
0481 GACTGCAGTC CAATCGCAGA GTATCAA--- -G |
| Assemble Data |
<PRE>
17894R-3.IR_full 1 GCAGTGGACATGTTAGCAACGGCTCCGGTAGCTCGGCACAACTTGGGGCGGGAAATCCGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17894R-3.in silico 1 GCAGTGGACATGTTAGCAACGGCTCCGGTAGCTCGGCACAACTTGGGGCGGGAAATCCGC 60
17894R-3.IR_full 61 ACGGTAACCAGGCCAACGGAGCGTCCGGCGGC<span class="snp"><tt>T</tt></span>GTTGGCTCAATGAGCGGATCAGCTGTG 120
||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||
17894R-3.in silico 61 ACGGTAACCAGGCCAACGGAGCGTCCGGCGGC<span class="snp"><tt>-</tt></span>GTTGGCTCAATGAGCGGATCAGCTGTG 120
17894R-3.IR_full 121 GGCGCTGGA<span class="snp"><tt>N</tt></span>CTACGGGAATGACCGCCGATCTCTTGGCAAGCGGCGGTGCAGGAGCACAA 180
|||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||
17894R-3.in silico 121 GGCGCTGGA<span class="snp"><tt>G</tt></span>CTACGGGAATGACCGCCGATCTCTTGGCAAGCGGCGGTGCAGGAGCACAA 180
17894R-3.IR_full 181 GGCGGTGCGGATCGCTTGGACGCGTCCAGCGACAGTGCTGTCAGTTCGATGGGCT<span class="snp"><tt>C</tt></span>CCGA 240
|||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||
17894R-3.in silico 181 GGCGGTGCGGATCGCTTGGACGCGTCCAGCGACAGTGCTGTCAGTTCGATGGGCT<span class="snp"><tt>-</tt></span>CCGA 240
17894R-3.IR_full 241 GCGA<span class="snp"><tt>T</tt></span>GTGCCGTCCCTCTCCGACGGCGAGTGGGGTGAGGGCA<span class="snp"><tt>T</tt></span>GTGA<span class="snp"><tt>T</tt></span>CTCCGCCCAGGA 300
||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||<span class="snp"><tt> </tt></span>||||||||||||
17894R-3.in silico 241 GCGA<span class="snp"><tt>-</tt></span>GTGCCGTCCCTCTCCGACGGCGAGTGGGGTGAGGGCA<span class="snp"><tt>-</tt></span>GTGA<span class="snp"><tt>-</tt></span>CTCCGCCCAGGA 300
17894R-3.IR_full 301 TTACCATCAGGGCAAGTACGGAGGCCCCTACGACTTCAGCTACAACAATAATTCACGGCT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17894R-3.in silico 301 TTACCATCAGGGCAAGTACGGAGGCCCCTACGACTTCAGCTACAACAATAATTCACGGCT 360
17894R-3.IR_full 361 TAGCACCGCCACACGTCAGCCGCCGGTGGCGCAGAAGAAGCATCAGCTGTACGGCAAGAG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17894R-3.in silico 361 TAGCACCGCCACACGTCAGCCGCCGGTGGCGCAGAAGAAGCATCAGCTGTACGGCAAGAG 420
17894R-3.IR_full 421 GGATCCCCATAAGCA<span class="snp"><tt>T</tt></span>GAC<span class="snp"><tt>A</tt></span>GCCATCGGCTTTGCC<span class="snp"><tt>G</tt></span>ACCAACAGCTCCACCAGCAGCCGC 480
|||||||||||||||<span class="snp"><tt> </tt></span>|||<span class="snp"><tt> </tt></span>|||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||
17894R-3.in silico 421 GGATCCCCATAAGCA<span class="snp"><tt>-</tt></span>GAC<span class="snp"><tt>-</tt></span>GCCATCGGCTTTGCC<span class="snp"><tt>-</tt></span>ACCAACAGCTCCACCAGCAGCCGC 480
17894R-3.IR_full 481 GACTGCAGTCCAATCGCAGAGTATCAA<span class="snp"><tt>A</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>T</tt></span>G 512
|||||||||||||||||||||||||||<span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span>|
17894R-3.in silico 481 GACTGCAGTCCAATCGCAGAGTATCAA<span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span>G 512
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
2 |
NM_170053.2 |
CG17894-RC, transcript variant C (cnc), mRNA |
| 100 |
482 |
0 |
0 |
2 |
NM_170054.2 |
CG17894-RB, transcript variant B (cnc), mRNA |
| 100 |
482 |
0 |
0 |
2 |
NM_170059.1 |
CG17894-RG, transcript variant G (cnc), mRNA |
| 100 |
482 |
0 |
0 |
2 |
NM_170058.1 |
CG17894-RF, transcript variant F (cnc), mRNA |
| 100 |
482 |
0 |
0 |
2 |
NM_170057.1 |
CG17894-RE, transcript variant E (cnc), mRNA |
| 100 |
482 |
0 |
0 |
2 |
NM_170056.1 |
CG17894-RD, transcript variant D (cnc), mRNA |
| 100 |
482 |
0 |
0 |
2 |
NM_170055.1 |
CG17894-RA, transcript variant A (cnc), mRNA |
| 0 |
0 |
3 |
3 |
9 |
NM_206456.1 |
CG32466-RB, transcript variant B (rn), mRNA |
| 0 |
0 |
2 |
8 |
8 |
NM_078684.2 |
CG3291-RA (pcm), mRNA |
| 0 |
0 |
2 |
5 |
4 |
NM_130728.1 |
CG15240-RA (CG15240), mRNA |
| 0 |
0 |
2 |
5 |
3 |
NM_176156.1 |
CG30044-RA (CG30044), mRNA |
| 0 |
0 |
2 |
3 |
10 |
NM_132569.1 |
CG11245-RA (CG11245), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_166335.2 |
CG7097-RA, transcript variant A (CG7097), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_167330.2 |
CG17762-RD, transcript variant D (tomosyn), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_167329.2 |
CG17762-RC, transcript variant C (tomosyn), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_167328.2 |
CG17762-RA, transcript variant A (tomosyn), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_132591.3 |
CG17762-RB, transcript variant B (tomosyn), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_078559.2 |
CG18085-RA (sev), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_132256.1 |
CG15350-RA (Cp7Fb), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_170307.1 |
CG6265-RB, transcript variant B (Nep5), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_143270.2 |
CG6265-RA, transcript variant A (Nep5), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_167210.1 |
CG32694-RC, transcript variant C (CG32694), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_167209.1 |
CG32694-RA, transcript variant A (CG32694), mRNA |
| 0 |
0 |
1 |
10 |
25 |
NM_169775.1 |
CG7467-RA, transcript variant A (osa), mRNA |
| 0 |
0 |
1 |
10 |
25 |
NM_079668.2 |
CG7467-RB, transcript variant B (osa), mRNA |
| 0 |
0 |
1 |
8 |
21 |
NM_206506.1 |
CG7467-RC, transcript variant C (osa), mRNA |
| 0 |
0 |
1 |
6 |
8 |
NM_134926.2 |
CG3304-RA, transcript variant A (CG3304), mRNA |
| 0 |
0 |
1 |
6 |
8 |
NM_205901.1 |
CG3304-RC, transcript variant C (CG3304), mRNA |
| 0 |
0 |
1 |
6 |
7 |
NM_166393.1 |
CG30143-RA (CG30143), mRNA |
| 0 |
0 |
1 |
6 |
7 |
NM_138229.1 |
CG13908-RA (CG13908), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
|
JNK signal
|
Caspase-3 signal
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Pathways (updated: 2024/04/26)
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| KEGG Pathway |
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