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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 17824R-3 
 Symbol CG17824  Full Name CG17824 
 CG No CG17824  Old CG No CG17824 
 Synonyms CG17824 
 Accession No (Link to NCBI) NM_140277.1 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgAGCGAACTGTTCCCAGTACGA 
 Primer Seq. 3' 
aatctagaggtaccGCTCCCGACTGAAGTACATGC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 AGCGAACTGT TCCCAGTACG AGATGTGCAT CGATGGTCGT TGGCGACGAC GCACTTGCTC 
0061 CGACCAGCGA TACTACAATC CGGAGCAGCA AAGATGCCTG GAGCCGCGAG ATGACTTGGT 
0121 ATGCGCCTAC GCAAGGGTAT CGAATATACC GACCTGCACC GCCATCAGCG AATCCATGAC 
0181 TATTCCCGCC AAAAGTGGCA ATTGTATGCA GTACTTCCGC TGCTCCGGTG GCAAATGGCG 
0241 TCTGCGCAAC TGTCCGAAGC AGCACTACTA CTCCCCGTTG ATAGGATCCT GTTTGCCTTT 
0301 CCCGCGGGGT CATGAGCAGG ACAGGAATCA AACTATTTGC ANTTGGGCGA TGAGCAAATT 
0361 GGCTGGTAAT TCCTCAGACG ACTGCCAGCA CCTGGCAGTG CGTCCTTCCT TGGCGGGCGG 
0421 CTGTCAGAGC TACCTAATGT GTCTGGACAA CTCTTGGTGG CTGCACCAGT GTCCCTTGGG 
0481 CATGTACTTC AGTCGGGAGC  
 in silico PCR Fragment 
0001 AGCGAACTGT TCCCAGTACG AGATGTGCAT CGATGGTCGT TGGCGACGAC GCACTTGCTC 
0061 CGACCAGCGA TACTACAATC CGGAGCAGCA AAGATGCCTG GAGCCGCGAG ATGACTTGGT 
0121 ATGCGCCTAC GCAAGGGTAT CGAATATACC GACCTGCACC GCCATCAGCG AATCCATGAC 
0181 TATTCCCGCC AAAAGTGGCA ATTGTATGCA GTACTTCCGC TGCTCCGGTG GCAAATGGCG 
0241 TCTGCGCAAC TGTCCGAAGC AGCACTACTA CTCCCCGTTG ATAGGATCCT GTTTGCCTTT 
0301 CCCGCGGGGT CATGAGCAGG ACAGGAATCA AACTATTTGC AGTTGGGCGA TGAGCAAATT 
0361 GGCTGGTAAT TCCTCAGACG ACTGCCAGCA CCTGGCAGTG CGTCCTTCCT TGGCGGGCGG 
0421 CTGTCAGAGC TACCTAATGT GTCTGGACAA CTCTTGGTGG CTGCACCAGT GTCCCTTGGG 
0481 CATGTACTTC AGTCGGGAGC  
 Assemble Data 
<PRE>

17824R-3.IR_full       1   AGCGAACTGTTCCCAGTACGAGATGTGCATCGATGGTCGTTGGCGACGACGCACTTGCTC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17824R-3.in silico     1   AGCGAACTGTTCCCAGTACGAGATGTGCATCGATGGTCGTTGGCGACGACGCACTTGCTC 60


17824R-3.IR_full       61  CGACCAGCGATACTACAATCCGGAGCAGCAAAGATGCCTGGAGCCGCGAGATGACTTGGT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17824R-3.in silico     61  CGACCAGCGATACTACAATCCGGAGCAGCAAAGATGCCTGGAGCCGCGAGATGACTTGGT 120


17824R-3.IR_full       121 ATGCGCCTACGCAAGGGTATCGAATATACCGACCTGCACCGCCATCAGCGAATCCATGAC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17824R-3.in silico     121 ATGCGCCTACGCAAGGGTATCGAATATACCGACCTGCACCGCCATCAGCGAATCCATGAC 180


17824R-3.IR_full       181 TATTCCCGCCAAAAGTGGCAATTGTATGCAGTACTTCCGCTGCTCCGGTGGCAAATGGCG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17824R-3.in silico     181 TATTCCCGCCAAAAGTGGCAATTGTATGCAGTACTTCCGCTGCTCCGGTGGCAAATGGCG 240


17824R-3.IR_full       241 TCTGCGCAACTGTCCGAAGCAGCACTACTACTCCCCGTTGATAGGATCCTGTTTGCCTTT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17824R-3.in silico     241 TCTGCGCAACTGTCCGAAGCAGCACTACTACTCCCCGTTGATAGGATCCTGTTTGCCTTT 300


17824R-3.IR_full       301 CCCGCGGGGTCATGAGCAGGACAGGAATCAAACTATTTGCA<span class="snp"><tt>N</tt></span>TTGGGCGATGAGCAAATT 360
                           |||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||
17824R-3.in silico     301 CCCGCGGGGTCATGAGCAGGACAGGAATCAAACTATTTGCA<span class="snp"><tt>G</tt></span>TTGGGCGATGAGCAAATT 360


17824R-3.IR_full       361 GGCTGGTAATTCCTCAGACGACTGCCAGCACCTGGCAGTGCGTCCTTCCTTGGCGGGCGG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17824R-3.in silico     361 GGCTGGTAATTCCTCAGACGACTGCCAGCACCTGGCAGTGCGTCCTTCCTTGGCGGGCGG 420


17824R-3.IR_full       421 CTGTCAGAGCTACCTAATGTGTCTGGACAACTCTTGGTGGCTGCACCAGTGTCCCTTGGG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17824R-3.in silico     421 CTGTCAGAGCTACCTAATGTGTCTGGACAACTCTTGGTGGCTGCACCAGTGTCCCTTGGG 480


17824R-3.IR_full       481 CATGTACTTCAGTCGGGAGC 500
                           ||||||||||||||||||||
17824R-3.in silico     481 CATGTACTTCAGTCGGGAGC 500

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  1  NM_140277.1  CG17824-RA (CG17824), mRNA 
0.2   1  2  2  5  NM_078859.2  CG5813-RA, transcript variant A (chif), mRNA 
0.2   1  2  2  2  NM_135951.2  CG4580-RA (CG4580), mRNA 
0.2   1  2  2  2  NM_165156.1  CG5813-RB, transcript variant B (chif), mRNA 
0   0  3  2  2  NM_169646.2  CG6236-RB, transcript variant B (CG6236), mRNA 
0   0  3  2  2  NM_142189.3  CG6236-RA, transcript variant A (CG6236), mRNA 
0   0  2  6  4  NM_080152.2  CG5092-RA (Tor), mRNA 
0   0  2  4  4  NM_139914.2  CG7565-RA (CG7565), mRNA 
0   0  2  4  3  NM_170181.1  CG31120-RB, transcript variant B (CG31120), mRNA 
0   0  2  3  2  NM_137071.3  CG13344-RA, transcript variant A (CG13344), mRNA 
0   0  2  3  2  NM_001032245.1  CG13344-RB, transcript variant B (CG13344), mRNA 
0   0  1  5  5  NM_142343.2  CG17475-RA (CG17475), mRNA 
0   0  1  3  5  NM_142886.1  CG4393-RA (CG4393), mRNA 
0   0  1  3  4  NM_164375.1  CG18497-RC, transcript variant C (spen), mRNA 
0   0  1  3  4  NM_164374.1  CG18497-RA, transcript variant A (spen), mRNA 
0   0  1  3  4  NM_079979.2  CG18497-RB, transcript variant B (spen), mRNA 
0   0  1  3  2  NM_136140.2  CG13082-RA (CG13082), mRNA 
0   0  1  2  4  NM_168894.1  CG9390-RA, transcript variant A (AcCoAS), mRNA 
0   0  1  2  2  NM_001014599.1  CG9390-RC, transcript variant C (AcCoAS), mRNA 
0   0  1  2  2  NM_142994.3  CG18528-RA (CG18528), mRNA 
0   0  1  2  2  NM_143524.2  CG9743-RA (CG9743), mRNA 
0   0  1  2  2  NM_079472.2  CG9390-RB, transcript variant B (AcCoAS), mRNA 
0   0  0  5  2  NM_206498.1  CG11648-RE, transcript variant E (Abd-B), mRNA 
0   0  0  5  2  NM_169735.1  CG11648-RC, transcript variant C (Abd-B), mRNA 
0   0  0  5  2  NM_142320.1  CG11648-RA, transcript variant A (Abd-B), mRNA 
0   0  0  5  2  NM_169736.1  CG11648-RD, transcript variant D (Abd-B), mRNA 
0   0  0  5  2  NM_080157.2  CG11648-RB, transcript variant B (Abd-B), mRNA 
0   0  0  4  7  NM_138759.2  CG9242-RA (bur), mRNA 
0   0  0  4  6  NM_136797.2  CG7737-RA (CG7737), mRNA 
0   0  0  4  5  NM_141324.1  CG1098-RA (Madm), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway

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