NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

17753R-1

Symbol

CCS

Full Name

CCS

CG No

CG17753

Old CG No

CG17753

Synonyms

CG17753, CCD, l(2)03221, anon-EST:Posey231, CCS

Accession No (Link to NCBI)

NM_143772.3

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Molnar C, Casado M, López-Varea A, Cruz C, de Celis JF.<br> Genetic annotation of gain-of-function screens using RNA interference and in situ hybridization of candidate genes in the Drosophila wing.<br> Genetics (2012) 192(2) 741-52 [ PubMed ID = 22798488 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAAGAGGTGACGAATCCTATGC

Primer Seq. 3'

aatctagaggtaccCAAATCTAAAGGTGGCCCTGC

Predicted Fragment Size

IR fragment full Seq

0001 AAGAGGTGAC GAATCCTATG CCGGTGCCCT CCGTTCGGCG CTGGATGGGG TCGGCCAGGT 
0061 GGAAATTGAC ACCCAGGAAG GTCGAGTGAT AATCCAAACC CAACGACCTT GGTCAGAGAT 
0121 TCAGGACAAG ATAGAAGCCA CAGGTGTCCG GGCCGTACTC TCCGGATTCG GCGGACAGTC 
0181 GGCGGTGGCC CTTATTAACA CAACAGGAAG TGTAGTGGAC AAGACACCAA TCCAGGGAGT 
0241 GGTTCGGTTT ACCACCATTA CCGCAGACAA GAAGCCTGGA GTGGTTGTGG ATGGCGTTGT 
0301 GGACGGTCTA TCGCCTGGAC TGCACGGATT ACATATTCAC GAGAGTGGTG ACACTTCCGC 
0361 AGGTTGCTCG TCGGTCGGAG AACACTACAA TCCCCGCCAG TCGCCACATG GAAGCCCTGC 
0421 AGCTGGGGCA GAGGAGCGGC ACGCCGGAGA TCTTGGCAAC ATCCGAGCGG ATGAAAACGG 
0481 CAGGGCCACC TTTAGATTTG

in silico PCR Fragment

0001 AAGAGGTGAC GAATCCTATG CCGGTGCCCT CCGTTCGGCG CTGGATGGGG TCGGCCAGGT 
0061 GGAAATTGAC ACCCAGGAAG GTCGAGTGAT AATCCAAACC CAACGACCTT GGTCAGAGAT 
0121 TCAGGACAAG ATAGAAGCCA CAGGTGTCCG GGCCGTACTC TCCGGATTCG GCGGCCAGTC 
0181 GGCGGTGGCC CTTATAAACA CAACAGGAAG TGTAGTGGAC AAGACACCAA TCCAGGGAGT 
0241 GGTTCGGTTT ACCACCATTA CCGCAGACAA GAAGCCTGGA GTGGTTGTGG ATGGCGTTGT 
0301 GGACGGTCTA TCGCCTGGAC TGCACGGATT ACATATTCAC GAGAGTGGTG ACACTTCCGC 
0361 AGGTTGCTCG TCGGTCGGAG AACACTACAA TCCCCGCCAG TCGCCACATG GAAGCCCTGC 
0421 AGCTGGGGCA GAGGAGCGGC ACGCCGGAGA TCTTGGCAAC ATCCGAGCGG ATGAAAACGG 
0481 CAGGGCCACC TTTAGATTTG

Assemble Data

<PRE>

17753R-1.IR_full       1   AAGAGGTGACGAATCCTATGCCGGTGCCCTCCGTTCGGCGCTGGATGGGGTCGGCCAGGT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17753R-1.in silico     1   AAGAGGTGACGAATCCTATGCCGGTGCCCTCCGTTCGGCGCTGGATGGGGTCGGCCAGGT 60


17753R-1.IR_full       61  GGAAATTGACACCCAGGAAGGTCGAGTGATAATCCAAACCCAACGACCTTGGTCAGAGAT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17753R-1.in silico     61  GGAAATTGACACCCAGGAAGGTCGAGTGATAATCCAAACCCAACGACCTTGGTCAGAGAT 120


17753R-1.IR_full       121 TCAGGACAAGATAGAAGCCACAGGTGTCCGGGCCGTACTCTCCGGATTCGGCGG<span class="snp"><tt>A</tt></span>CAGTC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||
17753R-1.in silico     121 TCAGGACAAGATAGAAGCCACAGGTGTCCGGGCCGTACTCTCCGGATTCGGCGG<span class="snp"><tt>C</tt></span>CAGTC 180


17753R-1.IR_full       181 GGCGGTGGCCCTTAT<span class="snp"><tt>T</tt></span>AACACAACAGGAAGTGTAGTGGACAAGACACCAATCCAGGGAGT 240
                           |||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||
17753R-1.in silico     181 GGCGGTGGCCCTTAT<span class="snp"><tt>A</tt></span>AACACAACAGGAAGTGTAGTGGACAAGACACCAATCCAGGGAGT 240


17753R-1.IR_full       241 GGTTCGGTTTACCACCATTACCGCAGACAAGAAGCCTGGAGTGGTTGTGGATGGCGTTGT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17753R-1.in silico     241 GGTTCGGTTTACCACCATTACCGCAGACAAGAAGCCTGGAGTGGTTGTGGATGGCGTTGT 300


17753R-1.IR_full       301 GGACGGTCTATCGCCTGGACTGCACGGATTACATATTCACGAGAGTGGTGACACTTCCGC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17753R-1.in silico     301 GGACGGTCTATCGCCTGGACTGCACGGATTACATATTCACGAGAGTGGTGACACTTCCGC 360


17753R-1.IR_full       361 AGGTTGCTCGTCGGTCGGAGAACACTACAATCCCCGCCAGTCGCCACATGGAAGCCCTGC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17753R-1.in silico     361 AGGTTGCTCGTCGGTCGGAGAACACTACAATCCCCGCCAGTCGCCACATGGAAGCCCTGC 420


17753R-1.IR_full       421 AGCTGGGGCAGAGGAGCGGCACGCCGGAGATCTTGGCAACATCCGAGCGGATGAAAACGG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17753R-1.in silico     421 AGCTGGGGCAGAGGAGCGGCACGCCGGAGATCTTGGCAACATCCGAGCGGATGAAAACGG 480


17753R-1.IR_full       481 CAGGGCCACCTTTAGATTTG 500
                           ||||||||||||||||||||
17753R-1.in silico     481 CAGGGCCACCTTTAGATTTG 500

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	3	NM_143772.3	CG17753-RA (CCS), mRNA
0	0	5	2	3	NM_079997.2	CG13580-RA (Crtp), mRNA
0	0	4	2	3	NM_136502.2	CG14762-RA (CG14762), mRNA
0	0	3	2	3	NM_141151.2	CG11370-RA (CG11370), mRNA
0	0	2	4	2	NM_166909.2	CG14814-RA, transcript variant A (CG14814), mRNA
0	0	2	4	2	NM_130591.2	CG14814-RB, transcript variant B (CG14814), mRNA
0	0	2	2	7	NM_141158.3	CG7369-RA (CG7369), mRNA
0	0	2	2	4	NM_140596.1	CG13071-RA (CG13071), mRNA
0	0	1	4	5	NM_140438.2	CG32139-RA (Sox21b), mRNA
0	0	1	3	10	NM_080110.2	CG32356-RA, transcript variant A (ImpE1), mRNA
0	0	1	3	5	NM_168270.1	CG32356-RB, transcript variant B (ImpE1), mRNA
0	0	1	3	3	NM_143018.2	CG5794-RD, transcript variant D (CG5794), mRNA
0	0	1	3	3	NM_170158.1	CG5794-RB, transcript variant B (CG5794), mRNA
0	0	1	3	3	NM_135123.2	CG9021-RA (CG9021), mRNA
0	0	1	3	2	NM_206564.1	CG5794-RE, transcript variant E (CG5794), mRNA
0	0	1	2	2	NM_168162.1	CG10173-RA (Best2), mRNA
0	0	1	2	2	NM_168571.2	CG32133-RA (CG32133), mRNA
0	0	0	6	7	NM_137133.2	CG10143-RA (Adgf-E), mRNA
0	0	0	6	3	NM_142043.1	CG9637-RA (Task6), mRNA
0	0	0	6	3	NM_132701.2	CG11092-RA (CG11092), mRNA
0	0	0	5	7	NM_139536.1	CG11505-RB, transcript variant B (CG11505), mRNA
0	0	0	5	7	NM_168007.1	CG11505-RA, transcript variant A (CG11505), mRNA
0	0	0	5	6	NM_206263.1	CG11505-RC, transcript variant C (CG11505), mRNA
0	0	0	5	1	NM_136145.1	CG13078-RA (CG13078), mRNA
0	0	0	4	8	NM_134507.1	CG14234-RA (CG14234), mRNA
0	0	0	4	7	NM_132955.1	CG4937-RA, transcript variant A (RhoGAP15B), mRNA
0	0	0	4	7	NM_001042817.1	CG4937-RB, transcript variant B (RhoGAP15B), mRNA
0	0	0	4	7	NM_140680.1	CG13022-RA (CG13022), mRNA
0	0	0	4	6	NM_137794.2	CG11073-RA (CG11073), mRNA
0	0	0	4	3	NM_206441.1	CG31481-RB, transcript variant B (pb), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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