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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 17633R-3

Symbol CG17633 Full Name CG17633

CG No CG17633 Old CG No CG17633

Synonyms CG17633

Accession No (Link to NCBI) NM_135466.2

Inserted Chr. X

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTCAACTCGGAGAATGCCAAGC

Primer Seq. 3'

aatctagaggtaccGATGATAAAGGTGGCGGCAGC

Predicted Fragment Size

IR fragment full Seq

0001 TCAACTCGGA GAATGCCAAG CAGCTGGAGG TGCTGAAGGA CCTCGAGGGA TCCAGCGACT 
0061 CAATCATGTT CTTGGATGGC GTTCATCTGG TTGGTGCCGA TATTCAGATC ATCGTGGCTC 
0121 CCCACAAGGT GCCCGATTTC CTAGAGATCC TGGGCAAGTC TGAAATCAAG TATGAGCTGC 
0181 AGTCGCGCGA TGTGCAGAAG TCTCTGGACG AGATCGACGA GAAGGTCGCT ATCAAGGGAC 
0241 GTGCCACCAC CGCCTACAAC TGGGCCGAGT ACTACGAACT CGACGACACC TATGCCTGGC 
0301 NCCAGTCCCT GGCCCAGACG AATCCCGGAG TGGTCACCCT CATTGAGGGC GGCAAGACCT 
0361 ACCAGGGACG CTCCATCCTC GGTGTTAAGA TCACCAAGGG AGGTGAGACC ATAAACGGCA 
0421 AgGCCAAGCC CGGCATCTTC CTGGAGGCCG GTATCCATGC CCGCGAGTGG ATTGCTCCAG 
0481 CTGCCNGCCA CCTTTATCAT C

in silico PCR Fragment

0001 TCAACTCGGA GAATGCCAAG CAACTGGAGG TGCTGAAGGA TCTCGAGGGA TCCAGCGACT 
0061 CAATCATGTT CTTGGATGGC GTTCATCTGG TTGGTGCCGA TATTCAGATA ATCGTGGCTC 
0121 CCCACAAGGT GCCCGACTTC CTGGAGATTC TGGGCAAGTC TGAAATCAAG TATGAGCTGC 
0181 AATCGCGCGA TGTGCAAAAG TCTCTGGACG AGATCGACGA GAAGGTCGCT ATCAAGGGAC 
0241 GTGCCACCAC CGCCTACAAC TGGGCCCAGT ACTACGAACT CGACGACACC TATGCCTGGC 
0301 TCCAGTCCCT GGCCCAGACG AATCCCGGAG TGGTCACCCT CATTGAGGGC GGCAAGACCT 
0361 ACCAGGGACG CTCCATCCTC GGTGTTAAGA TCACCAAGGG AGGTGAGACC ATAAACGGCA 
0421 AGGCCAAGCC CGGCATCTTC CTGGAGGCCG GTATCCATGC CCGCGAGTGG ATTGCTCCAG 
0481 CTGCC-GCCA CCTTTATCAT C

Assemble Data


17633R-3.IR_full       1   TCAACTCGGAGAATGCCAAGCAGCTGGAGGTGCTGAAGGACCTCGAGGGATCCAGCGACT 60
                           |||||||||||||||||||||| ||||||||||||||||| |||||||||||||||||||
17633R-3.in silico     1   TCAACTCGGAGAATGCCAAGCAACTGGAGGTGCTGAAGGATCTCGAGGGATCCAGCGACT 60


17633R-3.IR_full       61  CAATCATGTTCTTGGATGGCGTTCATCTGGTTGGTGCCGATATTCAGATCATCGTGGCTC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||
17633R-3.in silico     61  CAATCATGTTCTTGGATGGCGTTCATCTGGTTGGTGCCGATATTCAGATAATCGTGGCTC 120


17633R-3.IR_full       121 CCCACAAGGTGCCCGATTTCCTAGAGATCCTGGGCAAGTCTGAAATCAAGTATGAGCTGC 180
                           |||||||||||||||| ||||| ||||| |||||||||||||||||||||||||||||||
17633R-3.in silico     121 CCCACAAGGTGCCCGACTTCCTGGAGATTCTGGGCAAGTCTGAAATCAAGTATGAGCTGC 180


17633R-3.IR_full       181 AGTCGCGCGATGTGCAGAAGTCTCTGGACGAGATCGACGAGAAGGTCGCTATCAAGGGAC 240
                           | |||||||||||||| |||||||||||||||||||||||||||||||||||||||||||
17633R-3.in silico     181 AATCGCGCGATGTGCAAAAGTCTCTGGACGAGATCGACGAGAAGGTCGCTATCAAGGGAC 240


17633R-3.IR_full       241 GTGCCACCACCGCCTACAACTGGGCCGAGTACTACGAACTCGACGACACCTATGCCTGGC 300
                           |||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||
17633R-3.in silico     241 GTGCCACCACCGCCTACAACTGGGCCCAGTACTACGAACTCGACGACACCTATGCCTGGC 300


17633R-3.IR_full        301 CCAGTCCCTGGCCCAGACGAATCCCGGAGTGGTCACCCTCATTGAGGGCGGCAAGACCT 359
                            |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17633R-3.in silico      301 CCAGTCCCTGGCCCAGACGAATCCCGGAGTGGTCACCCTCATTGAGGGCGGCAAGACCT 359


17633R-3.IR_full       361 ACCAGGGACGCTCCATCCTCGGTGTTAAGATCACCAAGGGAGGTGAGACCATAAACGGCA 419
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17633R-3.in silico     361 ACCAGGGACGCTCCATCCTCGGTGTTAAGATCACCAAGGGAGGTGAGACCATAAACGGCA 419


17633R-3.IR_full       421 AGGCCAAGCCCGGCATCTTCCTGGAGGCCGGTATCCATGCCCGCGAGTGGATTGCTCCAG 479
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17633R-3.in silico     421 AGGCCAAGCCCGGCATCTTCCTGGAGGCCGGTATCCATGCCCGCGAGTGGATTGCTCCAG 479


17633R-3.IR_full       481 CTGCCNGCCACCTTTATCATC 500
                           ||||| |||||||||||||||
17633R-3.in silico     481 CTGCC-GCCACCTTTATCATC 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_135466.2	CG17633-RA (CG17633), mRNA
0	0	3	5	3	NM_138222.2	CG12191-RA (dpr20), mRNA
0	0	2	2	3	NM_001043101.1	CG3682-RC, transcript variant C (PIP5K59B), mRNA
0	0	2	2	3	NM_137885.2	CG3682-RA, transcript variant A (PIP5K59B), mRNA
0	0	2	2	3	NM_166567.1	CG3682-RB, transcript variant B (PIP5K59B), mRNA
0	0	2	2	2	NM_167558.1	CG32562-RA (xmas-2), mRNA
0	0	1	5	5	NM_137689.2	CG3221-RA (CG3221), mRNA
0	0	1	3	5	NM_165735.2	CG12919-RA, transcript variant A (egr), mRNA
0	0	1	3	5	NM_206069.1	CG12919-RB, transcript variant B (egr), mRNA
0	0	1	3	4	NM_136413.1	CG12836-RA (CG12836), mRNA
0	0	1	3	2	NM_135967.1	CG13272-RA (CG13272), mRNA
0	0	1	2	7	NM_136041.1	CG10231-RA (Pde11), mRNA
0	0	1	2	4	NM_136587.1	CG8230-RA (CG8230), mRNA
0	0	1	2	4	NM_138068.3	CG3483-RA (CG3483), mRNA
0	0	1	2	3	NM_164711.1	CG9261-RD, transcript variant D (nrv2), mRNA
0	0	1	2	3	NM_057821.3	CG9261-RA, transcript variant A (nrv2), mRNA
0	0	1	2	3	NM_001014476.1	CG9261-RE, transcript variant E (nrv2), mRNA
0	0	1	2	2	NM_168948.3	CG12673-RA (olf413), mRNA
0	0	1	2	2	NM_001038757.1	CG9201-RC, transcript variant C (Grip128), mRNA
0	0	1	2	2	NM_132802.2	CG9201-RB, transcript variant B (Grip128), mRNA
0	0	0	14	9	NM_139863.3	CG8560-RA (CG8560), mRNA
0	0	0	10	7	NM_142884.1	CG4408-RA (CG4408), mRNA
0	0	0	7	5	NM_165019.2	CG5792-RB, transcript variant B (CG5792), mRNA
0	0	0	7	3	NM_135746.2	CG5792-RA, transcript variant A (CG5792), mRNA
0	0	0	7	2	NM_138013.2	CG16787-RA (CG16787), mRNA
0	0	0	6	5	NM_165868.1	CG8857-RC, transcript variant C (RpS11), mRNA
0	0	0	6	5	NM_136903.3	CG8857-RA, transcript variant A (RpS11), mRNA
0	0	0	6	4	NM_142581.1	CG4562-RA (CG4562), mRNA
0	0	0	6	3	NM_139803.2	CG14820-RA (CG14820), mRNA
0	0	0	6	2	NM_168084.1	CG11347-RD, transcript variant D (CG11347), mRNA

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