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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 17324R-1

Symbol CG17324 Full Name CG17324

CG No CG17324 Old CG No CG17324

Synonyms GH06505, BEST:GH06505, CG17324

Accession No (Link to NCBI) NM_136065.3

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
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Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCTGGAGAAACCAGTGGCCAAC

Primer Seq. 3'

aatctagaggtaccTCAGCGATCAGGGCATCGGTG

Predicted Fragment Size

IR fragment full Seq

0001 CTGGAGAAAC CAGTGGCCAA CTACACGGAT TACGTCTTTC AAGGAATGCC TTTGCTCACC 
0061 GATATTGTGG ACTTAAGTAA CTTTGAATCC GAATGGAAGC CCCTGGGACT GCCGTTCAAG 
0121 GTGCCCACCT ACTTTATGCT TCACGATTGG GGTCTGCGAT CCTGTAAGGT GGCCCTGAAC 
0181 TCACCCCTCA TCACCCAACT GCTCAAGTCC CCCATTCGAT ATGACGTCAT ACTCCTGGAA 
0241 CACTTCTCCA ACGATTGCAT GGCANGCGGT GGCCCACCTA TTGAATGCAC CTGTAATCGC 
0301 ATTGAGTAGC TGTGCGANTA ATGCCGTGGC ACTATAAACG CATGGGAAGT CCGTTCATTA 
0361 ACCCCATTAT GCCCATGAAT TTCCTGCCCT ACACTGATGA GATGAGTCTG ATCGATCGAC 
0421 TGAATAATTT CTTTCATTTC CACACCGTTA ATACTCTGTA CAACATGATT ACCCAACCAG 
0481 CCACCGATGC CCTGATCGCT GA

in silico PCR Fragment

0001 CTGGAGAAAC CAGTGGCCAA CTACACGGAT TACGTCTTTC AAGGAATGCC CTTGCTCACC 
0061 GATATTGTGG ACTTAAGTAA CTTTGAATCC GAATGGAAGC CCCTGGGACT GCCGTTCAAG 
0121 GTGCCCACCT ACTTTATGCT TCACGATTGG GGTCTGCGAT CCTGTAAGGT GGCCCTGAAC 
0181 TCACCCCTCA TCACCCAACT GCTCAAGTCC CCCATTCGAT ATGACGTCAT ACTCCTGGAA 
0241 CACTTCTCCA ACGATTGCAT GGCA-GCGGT GGCCCACCTA TTGAATGCAC CTGTAATCGC 
0301 ATTGAGTAGC TGTGCGA-TA ATGCCGTGGC ACTACAAACG CATGGGAAGT CCGTTCATTA 
0361 ACCCCATTAT GCCCATGAAT TTCCTGCCCT ACACTGATGA GATGAGTCTG ATCGATCGAC 
0421 TGAATAATTT CTTTCATTTC CACACCGTTA ATACTCTGTA CAACATGATT ACCCAACCAG 
0481 CCACCGATGC CCTGATCGCT GA

Assemble Data


17324R-1.IR_full       1   CTGGAGAAACCAGTGGCCAACTACACGGATTACGTCTTTCAAGGAATGCCTTTGCTCACC 60
                           |||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||
17324R-1.in silico     1   CTGGAGAAACCAGTGGCCAACTACACGGATTACGTCTTTCAAGGAATGCCCTTGCTCACC 60


17324R-1.IR_full       61  GATATTGTGGACTTAAGTAACTTTGAATCCGAATGGAAGCCCCTGGGACTGCCGTTCAAG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17324R-1.in silico     61  GATATTGTGGACTTAAGTAACTTTGAATCCGAATGGAAGCCCCTGGGACTGCCGTTCAAG 120


17324R-1.IR_full       121 GTGCCCACCTACTTTATGCTTCACGATTGGGGTCTGCGATCCTGTAAGGTGGCCCTGAAC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17324R-1.in silico     121 GTGCCCACCTACTTTATGCTTCACGATTGGGGTCTGCGATCCTGTAAGGTGGCCCTGAAC 180


17324R-1.IR_full       181 TCACCCCTCATCACCCAACTGCTCAAGTCCCCCATTCGATATGACGTCATACTCCTGGAA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17324R-1.in silico     181 TCACCCCTCATCACCCAACTGCTCAAGTCCCCCATTCGATATGACGTCATACTCCTGGAA 240


17324R-1.IR_full       241 CACTTCTCCAACGATTGCATGGCANGCGGTGGCCCACCTATTGAATGCACCTGTAATCGC 300
                           |||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||
17324R-1.in silico     241 CACTTCTCCAACGATTGCATGGCA-GCGGTGGCCCACCTATTGAATGCACCTGTAATCGC 300


17324R-1.IR_full       301 ATTGAGTAGCTGTGCGANTAATGCCGTGGCACTATAAACGCATGGGAAGTCCGTTCATTA 360
                           ||||||||||||||||| |||||||||||||||| |||||||||||||||||||||||||
17324R-1.in silico     301 ATTGAGTAGCTGTGCGA-TAATGCCGTGGCACTACAAACGCATGGGAAGTCCGTTCATTA 360


17324R-1.IR_full       361 ACCCCATTATGCCCATGAATTTCCTGCCCTACACTGATGAGATGAGTCTGATCGATCGAC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17324R-1.in silico     361 ACCCCATTATGCCCATGAATTTCCTGCCCTACACTGATGAGATGAGTCTGATCGATCGAC 420


17324R-1.IR_full       421 TGAATAATTTCTTTCATTTCCACACCGTTAATACTCTGTACAACATGATTACCCAACCAG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17324R-1.in silico     421 TGAATAATTTCTTTCATTTCCACACCGTTAATACTCTGTACAACATGATTACCCAACCAG 480


17324R-1.IR_full       481 CCACCGATGCCCTGATCGCTGA 502
                           ||||||||||||||||||||||
17324R-1.in silico     481 CCACCGATGCCCTGATCGCTGA 502

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_136065.3	CG17324-RA (CG17324), mRNA
0	0	2	2	2	NM_141960.1	CG11600-RA (CG11600), mRNA
0	0	1	4	3	NM_206220.1	CG6845-RB, transcript variant B (CG6845), mRNA
0	0	1	4	3	NM_138156.2	CG6845-RA, transcript variant A (CG6845), mRNA
0	0	1	2	4	NM_141371.2	CG15592-RA (Osi9), mRNA
0	0	1	1	2	NM_138264.1	CG9173-RA (CG9173), mRNA
0	0	0	6	3	NM_170232.1	CG5071-RA, transcript variant A (CG5071), mRNA
0	0	0	6	3	NM_143149.1	CG5071-RB, transcript variant B (CG5071), mRNA
0	0	0	5	11	NM_168357.2	CG32045-RB, transcript variant B (fry), mRNA
0	0	0	5	8	NM_057393.3	CG3158-RA (spn-E), mRNA
0	0	0	5	4	NM_078783.2	CG7068-RA (TepIII), mRNA
0	0	0	4	32	NM_136066.2	CG17323-RA (CG17323), mRNA
0	0	0	4	4	NM_167553.1	CG9059-RA, transcript variant A (CG9059), mRNA
0	0	0	3	9	NM_057969.3	CG9635-RD, transcript variant D (RhoGEF2), mRNA
0	0	0	3	9	NM_206147.1	CG9635-RE, transcript variant E (RhoGEF2), mRNA
0	0	0	3	9	NM_206146.1	CG9635-RF, transcript variant F (RhoGEF2), mRNA
0	0	0	3	7	NM_137730.2	CG30387-RA, transcript variant A (CG30387), mRNA
0	0	0	3	7	NM_140828.2	CG3797-RA (CG3797), mRNA
0	0	0	3	6	NM_142959.2	CG6129-RB, transcript variant B (CG6129), mRNA
0	0	0	3	6	NM_168143.1	CG32408-RA (CG32408), mRNA
0	0	0	3	6	NM_132120.1	CG14438-RA, transcript variant A (CG14438), mRNA
0	0	0	3	5	NM_167940.1	CG32305-RA (CG32305), mRNA
0	0	0	3	5	NM_132479.2	CG1597-RA (CG1597), mRNA
0	0	0	3	4	NM_137136.1	CG12866-RA (CG12866), mRNA
0	0	0	3	4	NM_057345.2	CG10325-RA, transcript variant A (abd-A), mRNA
0	0	0	3	4	NM_169733.2	CG10325-RB, transcript variant B (abd-A), mRNA
0	0	0	3	3	NM_141979.1	CG10909-RA (CG10909), mRNA
0	0	0	3	3	NM_057938.3	CG3093-RA (dor), mRNA
0	0	0	3	3	NM_140905.2	CG7823-RA (RhoGDI), mRNA
0	0	0	3	3	NM_144328.2	CG10570-RA (CG10570), mRNA

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