NIG-Fly - Fly Stocks of National Institute of Genetics -

NIG-RNAi Strains Detail

Request : Click the "Order" button to request for this stock

temporarily unavailable

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 17321R-4

Symbol CG17321 Full Name CG17321

CG No CG17321 Old CG No CG17321

Synonyms CG17321

Accession No (Link to NCBI) NM_136071.2

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGGTCTGCTGTCAGCTGGACGA

Primer Seq. 3'

aatctagaggtaccGGACAAGCAGTGTGGAGCTCA

Predicted Fragment Size

IR fragment full Seq

0001 GGTCTGCTGT CAGCTGGACG ATGAGCAGGA CTTTGAAATA GCTGGCAGCA GGGCCGGCGA 
0061 GCTCTCCCAC TCGCACTCGC ACTCGCTGTC CTTGGAATCC CCCCTCGGAA CCTGCACCTC 
0121 CTGGGACTCC CATGCCAACT ACAAGCAGAG GGGCGGCCAC ACCCCCCTGC CCTgGGGCGT 
0181 CGCCCTTCAC TGTGATCCGC AACACTTGAA ATCGCCTACA GGGATTGTGC GCATACTATT 
0241 GGTGTTATCC TCGGCTGCCT GCCTGGCCTG TGAATGCTCC GCGGGCACCG TACAGGTGGG 
0301 CCTCTTTCTA CTGCCACTCA TCGGACGTCC TGAGGCTGAT GGTCTTCTGC GCGCTCTTTT 
0361 CGCTACTCAT CACGTGTCTC ATGCTCTTTC TGGATATATC GCATATAGCC CTCATGTTCC 
0421 CATTCAACTG GACAAAAGTG AATACGTGGA TGTACCTGAG TGTTGGTTTG ATATTTATTT 
0481 TGAGCTCCAC ACTGCTTGTC C

in silico PCR Fragment

0001 GGTCTGCTGT CAGCTGGACG ATGAGCAGGA CTTTGAAATA GCTGGCAGCA GGGCCGGCGA 
0061 GCTCTCCCAC TCGCACTCGC ACTCGCTGTC CTTGGAATCC CCCCTCGGAA CCTGCACCTC 
0121 CTGGGACTCC CATGCCAACT ACAAGCAGAG GGGCGGCCAC ACCCCCCTGC CCTGGGGCGT 
0181 CGCCCTTCAC TGTGATCCGC AACACTTGAA ATCGCCTACA GGGATTGTGC GCATACTATT 
0241 GGTGTTATCC TCGGCTGCCT GCCTGGCCTG TGAATGCTCC GCGGGCACCG TACAGGTGGG 
0301 CCTCTTTCTA CTGCCACTCA TCGGACG-CC TGAGGCTGAT GGTCTTCTGC GCGCTCTTTT 
0361 CGCTACTCAT CACGTGTCTC ATGCTCTTTC TGGATATATC GCATATAGCC CTCATGTTCC 
0421 CATTCAACTG GACAAAAGTG AATACGTGGA TGTACCTGAG TGTTGGTTTG ATATTTATTT 
0481 TGAGCTCCAC ACTGCTTGTC C

Assemble Data


17321R-4.IR_full       1   GGTCTGCTGTCAGCTGGACGATGAGCAGGACTTTGAAATAGCTGGCAGCAGGGCCGGCGA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17321R-4.in silico     1   GGTCTGCTGTCAGCTGGACGATGAGCAGGACTTTGAAATAGCTGGCAGCAGGGCCGGCGA 60


17321R-4.IR_full       61  GCTCTCCCACTCGCACTCGCACTCGCTGTCCTTGGAATCCCCCCTCGGAACCTGCACCTC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17321R-4.in silico     61  GCTCTCCCACTCGCACTCGCACTCGCTGTCCTTGGAATCCCCCCTCGGAACCTGCACCTC 120


17321R-4.IR_full       121 CTGGGACTCCCATGCCAACTACAAGCAGAGGGGCGGCCACACCCCCCTGCCCTGGGGCGT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17321R-4.in silico     121 CTGGGACTCCCATGCCAACTACAAGCAGAGGGGCGGCCACACCCCCCTGCCCTGGGGCGT 180


17321R-4.IR_full       181 CGCCCTTCACTGTGATCCGCAACACTTGAAATCGCCTACAGGGATTGTGCGCATACTATT 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17321R-4.in silico     181 CGCCCTTCACTGTGATCCGCAACACTTGAAATCGCCTACAGGGATTGTGCGCATACTATT 240


17321R-4.IR_full       241 GGTGTTATCCTCGGCTGCCTGCCTGGCCTGTGAATGCTCCGCGGGCACCGTACAGGTGGG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17321R-4.in silico     241 GGTGTTATCCTCGGCTGCCTGCCTGGCCTGTGAATGCTCCGCGGGCACCGTACAGGTGGG 300


17321R-4.IR_full       301 CCTCTTTCTACTGCCACTCATCGGACGTCCTGAGGCTGATGGTCTTCTGCGCGCTCTTTT 360
                           ||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||
17321R-4.in silico     301 CCTCTTTCTACTGCCACTCATCGGACG-CCTGAGGCTGATGGTCTTCTGCGCGCTCTTTT 360


17321R-4.IR_full       361 CGCTACTCATCACGTGTCTCATGCTCTTTCTGGATATATCGCATATAGCCCTCATGTTCC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17321R-4.in silico     361 CGCTACTCATCACGTGTCTCATGCTCTTTCTGGATATATCGCATATAGCCCTCATGTTCC 420


17321R-4.IR_full       421 CATTCAACTGGACAAAAGTGAATACGTGGATGTACCTGAGTGTTGGTTTGATATTTATTT 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17321R-4.in silico     421 CATTCAACTGGACAAAAGTGAATACGTGGATGTACCTGAGTGTTGGTTTGATATTTATTT 480


17321R-4.IR_full       481 TGAGCTCCACACTGCTTGTCC 501
                           |||||||||||||||||||||
17321R-4.in silico     481 TGAGCTCCACACTGCTTGTCC 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	2	10	NM_136071.2	CG17321-RA (CG17321), mRNA
0.2	1	8	10	12	NM_079577.3	CG6281-RA, transcript variant A (Timp), mRNA
0.2	1	8	10	12	NM_169336.2	CG6281-RB, transcript variant B (Timp), mRNA
0.2	1	3	2	3	NM_166823.1	CG11059-RB, transcript variant B (cals), mRNA
0	0	5	6	9	NM_001043266.1	CG34119-RA (CG34119), mRNA
0	0	3	7	6	NM_206631.1	CG10706-RF, transcript variant F (SK), mRNA
0	0	3	7	6	NM_167029.1	CG10706-RC, transcript variant C (SK), mRNA
0	0	3	7	5	NM_167032.1	CG10706-RB, transcript variant B (SK), mRNA
0	0	3	7	5	NM_167031.1	CG10706-RA, transcript variant A (SK), mRNA
0	0	3	4	4	NM_166189.1	CG30463-RB, transcript variant B (CG30463), mRNA
0	0	3	4	4	NM_166188.1	CG30463-RA, transcript variant A (CG30463), mRNA
0	0	3	2	6	NM_167336.1	CG4004-RB, transcript variant B (CG4004), mRNA
0	0	3	2	6	NM_132596.1	CG4004-RA, transcript variant A (CG4004), mRNA
0	0	3	2	3	NM_140304.2	CG5620-RA, transcript variant A (CG5620), mRNA
0	0	3	2	3	NM_206343.1	CG5620-RB, transcript variant B (CG5620), mRNA
0	0	2	8	8	NM_139875.3	CG7546-RA, transcript variant A (CG7546), mRNA
0	0	2	8	8	NM_168217.2	CG7546-RB, transcript variant B (CG7546), mRNA
0	0	2	8	3	NM_165622.1	CG8411-RA (gcl), mRNA
0	0	2	5	5	NM_134618.2	CG14619-RA, transcript variant A (CG14619), mRNA
0	0	2	5	5	NM_167775.1	CG14619-RD, transcript variant D (CG14619), mRNA
0	0	2	5	5	NM_167776.1	CG14619-RE, transcript variant E (CG14619), mRNA
0	0	2	3	8	NM_078549.3	CG32688-RA, transcript variant A (Hk), mRNA
0	0	2	2	5	NM_078742.2	CG3289-RA (Ptpa), mRNA
0	0	2	2	2	NM_136591.3	CG8213-RA (CG8213), mRNA
0	0	1	9	8	NM_134290.1	CG3090-RB, transcript variant B (Sox14), mRNA
0	0	1	9	8	NM_057546.2	CG3090-RA, transcript variant A (Sox14), mRNA
0	0	1	8	11	NM_134553.2	CG1812-RA, transcript variant A (CG1812), mRNA
0	0	1	5	11	NM_057400.3	CG6993-RA (ss), mRNA
0	0	1	4	5	NM_079507.2	CG2530-RA (corto), mRNA
0	0	1	4	2	NM_137482.1	CG17525-RA (GstE4), mRNA

Back