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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 17245R-4 
 Symbol plexB  Full Name plexin B 
 CG No CG17245  Old CG No CG17245 
 Synonyms PlexB, plex, unnamed, CG17245, plexB 
 Accession No (Link to NCBI) NM_079877.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees semi-lethal 
 Map Viewer
 Reference
[Please submit your publication]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgTCGGTTCTCAGCATTATTGCG 
 Primer Seq. 3'
aatctagaggtaccAACTGCAAAAAACTGGGGCGT 
 Predicted Fragment Size
500 
 IR fragment full Seq
0001 TCGGTTCTCA GCATTATTGC GTTAACTGCA TTGAAGAACT GCCGGCTCAG GCGTTATCTC 
0061 CCTTAAATGA CATAGTCGCC CAGTTTAACT TGCCATCAAT ACCAGTTCAA TCAACAGCTT 
0121 CCAGCTATGG CAATCGATCA ATTGGAAACA ATATAGAAAG TGTTAGAGAC TCTCAATCTA 
0181 AAAATTATTT TACTCACATG AGCTTCGACT TTATGCACAA TGTTTTGTTT GCGGGTGCTA 
0241 CAAATAAAAT ACTTAAACTT AACGAAAACC TTCGAGTGCT GGCCGAGGCT GTTACTGGGC 
0301 CGTTACATGA TTCCCCGCAA TGTCACGCTG GAGGCTGTCC GGAAGATATT GAGACATCGC 
0361 TTGTCAATAA CTTCAACAAA ATTCTAGTAN GTAAGCTACG CTCACGATGG TATTCTCATA 
0421 GCGTGTGGAA GCATTCGACA AGGAGCCTGC GAAATTTACA GCCTGCCACG TTTTCCGGCA 
0481 ACGCCCCAGT TTTTTGCAGT T 
 in silico PCR Fragment
0001 TCGGTTCTCA GCATTATTGC GTTAACTGCA TTGAAGAACT GCCGGCTCAG GCGTTATCTC 
0061 CCTTAAATGA CATAGTCGCC CAGTTTAACT TGCCATCAAT ACCAGTTCAA TCAACAGCTT 
0121 CCAGCTATGG CAATCGATCA ATTGGAAACA ATATAGAAAG TGTTAGAGAC TCTCAATCTA 
0181 AAAATTATTT TACTCACATG AGCTTCGACT TTATGCACAA TGTTTTGTTT GCGGGTGCTA 
0241 CAAATAAAAT ACTTAAACTT AACGAAAACC TTCGAGTGCT GGCCGAGGCT GTTACTGGGC 
0301 CGTTACATGA TTCCCCGCAA TGTCACGCTG GAGGCTGTCC GGAAGATATT GAGACATCGC 
0361 TTGTCAATAA CTTCAACAAA ATTCTAGTA- GTAAGCTACG CTCACGATGG TATTCTCATA 
0421 GCGTGTGGAA GCATTCGACA AGGAGCCTGC GAAATTTACA GCCTGCCACG TTTTCCGGCA 
0481 ACGCCCCAGT TTTTTGCAGT T 
 Assemble Data

17245R-4.IR_full       1   TCGGTTCTCAGCATTATTGCGTTAACTGCATTGAAGAACTGCCGGCTCAGGCGTTATCTC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17245R-4.in silico     1   TCGGTTCTCAGCATTATTGCGTTAACTGCATTGAAGAACTGCCGGCTCAGGCGTTATCTC 60


17245R-4.IR_full       61  CCTTAAATGACATAGTCGCCCAGTTTAACTTGCCATCAATACCAGTTCAATCAACAGCTT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17245R-4.in silico     61  CCTTAAATGACATAGTCGCCCAGTTTAACTTGCCATCAATACCAGTTCAATCAACAGCTT 120


17245R-4.IR_full       121 CCAGCTATGGCAATCGATCAATTGGAAACAATATAGAAAGTGTTAGAGACTCTCAATCTA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17245R-4.in silico     121 CCAGCTATGGCAATCGATCAATTGGAAACAATATAGAAAGTGTTAGAGACTCTCAATCTA 180


17245R-4.IR_full       181 AAAATTATTTTACTCACATGAGCTTCGACTTTATGCACAATGTTTTGTTTGCGGGTGCTA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17245R-4.in silico     181 AAAATTATTTTACTCACATGAGCTTCGACTTTATGCACAATGTTTTGTTTGCGGGTGCTA 240


17245R-4.IR_full       241 CAAATAAAATACTTAAACTTAACGAAAACCTTCGAGTGCTGGCCGAGGCTGTTACTGGGC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17245R-4.in silico     241 CAAATAAAATACTTAAACTTAACGAAAACCTTCGAGTGCTGGCCGAGGCTGTTACTGGGC 300


17245R-4.IR_full       301 CGTTACATGATTCCCCGCAATGTCACGCTGGAGGCTGTCCGGAAGATATTGAGACATCGC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17245R-4.in silico     301 CGTTACATGATTCCCCGCAATGTCACGCTGGAGGCTGTCCGGAAGATATTGAGACATCGC 360


17245R-4.IR_full       361 TTGTCAATAACTTCAACAAAATTCTAGTANGTAAGCTACGCTCACGATGGTATTCTCATA 420
                           ||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||
17245R-4.in silico     361 TTGTCAATAACTTCAACAAAATTCTAGTA-GTAAGCTACGCTCACGATGGTATTCTCATA 420


17245R-4.IR_full       421 GCGTGTGGAAGCATTCGACAAGGAGCCTGCGAAATTTACAGCCTGCCACGTTTTCCGGCA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17245R-4.in silico     421 GCGTGTGGAAGCATTCGACAAGGAGCCTGCGAAATTTACAGCCTGCCACGTTTTCCGGCA 480


17245R-4.IR_full       481 ACGCCCCAGTTTTTTGCAGTT 501
                           |||||||||||||||||||||
17245R-4.in silico     481 ACGCCCCAGTTTTTTGCAGTT 501

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  NM_079877.2  CG17245-RA (plexB), mRNA 
0   NM_143432.2  CG31445-RA (CG31445), mRNA 
0   NM_132859.2  CG9066-RA (CG9066), mRNA 
0   12  NM_164517.1  CG8817-RC, transcript variant C (lilli), mRNA 
0   NM_078740.2  CG8817-RB, transcript variant B (lilli), mRNA 
0   NM_164516.1  CG8817-RA, transcript variant A (lilli), mRNA 
0   NM_143567.3  CG11315-RA, transcript variant A (CG11315), mRNA 
0   NM_170521.2  CG11315-RB, transcript variant B (CG11315), mRNA 
0   NM_141562.1  CG11760-RB, transcript variant B (CG11760), mRNA 
0   NM_169236.1  CG11760-RA, transcript variant A (CG11760), mRNA 
0   NM_057515.2  CG6518-RA (inaC), mRNA 
0   NM_176153.1  CG33013-RB (CG33013), mRNA 
0   NM_142034.1  CG9764-RA (yrt), mRNA 
0   NM_078867.2  CG5526-RA (Dhc36C), mRNA 
0   NM_167821.1  CG32476-RA (mthl14), mRNA 
0   NM_168386.1  CG8108-RA, transcript variant A (CG8108), mRNA 
0   NM_140110.2  CG8108-RB, transcript variant B (CG8108), mRNA 
0   NM_140048.2  CG4080-RA (CG4080), mRNA 
0   NM_135355.2  CG8282-RA (Snx6), mRNA 
0   NM_141289.2  CG2519-RA, transcript variant A (CG2519), mRNA 
0   NM_139791.1  CG10144-RA (CG10144), mRNA 
0   NM_168773.1  CG4144-RC, transcript variant C (GNBP2), mRNA 
0   NM_079417.2  CG4144-RB, transcript variant B (GNBP2), mRNA 
0   NM_168772.1  CG4144-RD, transcript variant D (GNBP2), mRNA 
0   NM_168771.1  CG4144-RA, transcript variant A (GNBP2), mRNA 
0   NM_136807.1  CG13226-RA (CG13226), mRNA 
0   NM_166704.1  CG15792-RB, transcript variant B (zip), mRNA 
0   NM_001014553.1  CG15792-RC, transcript variant C (zip), mRNA 
0   NM_079136.2  CG15792-RA, transcript variant A (zip), mRNA 
0   NM_001014552.1  CG15792-RD, transcript variant D (zip), mRNA 
 Wing Disc
GFP
Puc
Caspase
DAPI
Merge
Figure
JNK signal
N
 
DB
 
DC
 
DM
 
VM
 
VB
 
Caspase-3 signal
N
DB
DC
++ 
DM
VM
 
VB
 
Dcompartment   Penetrance
     

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