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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 17239R-3

Symbol CG17239 Full Name CG17239

CG No CG17239 Old CG No CG17239

Synonyms SP138, CG17239

Accession No (Link to NCBI) NM_164469.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGATTTTCCTGGCCTTCAGCGT

Primer Seq. 3'

aatctagaggtaccGACACTGATCCTGGTCCACAA

Predicted Fragment Size

IR fragment full Seq

0001 NATTTTCCTG GCCTTCAGCG TCACCGTGGT TTCCTCTAAT TGGATTCCGG AACGAATCGT 
0061 GGGAGGCGAC TTGATAACCA TTCTATCAGT TNCCCTGGCA GGCTTCCATT CTTAGGCGTG 
0121 GACGGTTTCA CTGCGGTGCA GCCNATCNTA CAGTGAAGAC ATCGTCATAA CGGCAGCCCA 
0181 CTGCCTCACC GACCGCGAGA CCGAGTTCCT CTCAGTGAGA GTCGGCTCAT CGTTCACATT 
0241 CTTCGGTGGT CAGGTGGTGA GGGTATCCAG GGTTCTGTTA CACGAGGAGT ACGACCAGTC 
0301 CTGGTCCAAC GACATAGCCG TGATGAGGCT TCAGTCCAAG CTCCGGCTGG GAAGTGCAGT 
0361 CAGTGTCATT CCCTTGGCCG ATACTCCTCC AGCCAGTGGA TCACCTGCCA CCGTTTCTGG 
0421 ATGGGGAGCC ATTGGTTATA AGAAGAATTA CCCCATATCC ATACTGTCCG CTTCGGTGGA 
0481 CATTGTGGAC CAGGATCAGT GTC

in silico PCR Fragment

0001 GATTTTCCTG GCCTTCAGCG TCACCGTGGT TTCCTCTAAT TGGATTCCGG AACGAATCGT 
0061 GGGAGGCGAC TTGATAACCA TTCTATCAGT T-CCCTGGCA GGCTTCCATT CTTAGGCTTG 
0121 GACGGTTTCA CTGCGGTGCA GCC-ATC-TA CAGTGAAGAC ATCGTCATAA CGGCAGCCCA 
0181 CTGCCTCACC GACCGCGAGA CCGAGTTCCT CTCAGTGAGA GTCGGCTCAT CGTTCACATT 
0241 CTTCGGTGGT CAGGTGGTGA GGGTATCCAG TGTTCTGTTA CACGAGGAAT ACGACCAGTC 
0301 CTGGTCCAAC GACATAGCCG TGATGAGGCT TCAGTCCAAG CTCCGGCTGG GAAGTGCAGT 
0361 CAGTGTCATT CCCTTGGCCG ATACTCCCCC GGCCAGTGGA TCACCTGCCA CCGTTTCTGG 
0421 ATGGGGAGCC ATTGGTTTTA AGAAGAATTA CCCCATGTCC ATACTGTCCG CTTCGGTGGA 
0481 CATTGTGGAC CAGGATCAGT GTC

Assemble Data


17239R-3.IR_full        1   ATTTTCCTGGCCTTCAGCGTCACCGTGGTTTCCTCTAATTGGATTCCGGAACGAATCGT 59
                            |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17239R-3.in silico      1   ATTTTCCTGGCCTTCAGCGTCACCGTGGTTTCCTCTAATTGGATTCCGGAACGAATCGT 59


17239R-3.IR_full       61  GGGAGGCGACTTGATAACCATTCTATCAGTTNCCCTGGCAGGCTTCCATTCTTAGGCGTG 119
                           ||||||||||||||||||||||||||||||| ||||||||||||||||||||||||| ||
17239R-3.in silico     61  GGGAGGCGACTTGATAACCATTCTATCAGTT-CCCTGGCAGGCTTCCATTCTTAGGCTTG 119


17239R-3.IR_full       121 GACGGTTTCACTGCGGTGCAGCCNATCNTACAGTGAAGACATCGTCATAACGGCAGCCCA 179
                           ||||||||||||||||||||||| ||| ||||||||||||||||||||||||||||||||
17239R-3.in silico     121 GACGGTTTCACTGCGGTGCAGCC-ATC-TACAGTGAAGACATCGTCATAACGGCAGCCCA 179


17239R-3.IR_full       181 CTGCCTCACCGACCGCGAGACCGAGTTCCTCTCAGTGAGAGTCGGCTCATCGTTCACATT 239
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17239R-3.in silico     181 CTGCCTCACCGACCGCGAGACCGAGTTCCTCTCAGTGAGAGTCGGCTCATCGTTCACATT 239


17239R-3.IR_full       241 CTTCGGTGGTCAGGTGGTGAGGGTATCCAGGGTTCTGTTACACGAGGAGTACGACCAGTC 299
                           |||||||||||||||||||||||||||||| ||||||||||||||||| |||||||||||
17239R-3.in silico     241 CTTCGGTGGTCAGGTGGTGAGGGTATCCAGTGTTCTGTTACACGAGGAATACGACCAGTC 299


17239R-3.IR_full       301 CTGGTCCAACGACATAGCCGTGATGAGGCTTCAGTCCAAGCTCCGGCTGGGAAGTGCAGT 359
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17239R-3.in silico     301 CTGGTCCAACGACATAGCCGTGATGAGGCTTCAGTCCAAGCTCCGGCTGGGAAGTGCAGT 359


17239R-3.IR_full       361 CAGTGTCATTCCCTTGGCCGATACTCCTCCAGCCAGTGGATCACCTGCCACCGTTTCTGG 419
                           ||||||||||||||||||||||||||| || |||||||||||||||||||||||||||||
17239R-3.in silico     361 CAGTGTCATTCCCTTGGCCGATACTCCCCCGGCCAGTGGATCACCTGCCACCGTTTCTGG 419


17239R-3.IR_full       421 ATGGGGAGCCATTGGTTATAAGAAGAATTACCCCATATCCATACTGTCCGCTTCGGTGGA 479
                           ||||||||||||||||| |||||||||||||||||| |||||||||||||||||||||||
17239R-3.in silico     421 ATGGGGAGCCATTGGTTTTAAGAAGAATTACCCCATGTCCATACTGTCCGCTTCGGTGGA 479


17239R-3.IR_full       481 CATTGTGGACCAGGATCAGTGTC 502
                           |||||||||||||||||||||||
17239R-3.in silico     481 CATTGTGGACCAGGATCAGTGTC 502

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_164469.1	CG17239-RA (CG17239), mRNA
0	0	4	3	4	NM_169839.1	CG17836-RA, transcript variant A (CG17836), mRNA
0	0	4	3	4	NM_142504.2	CG17836-RB, transcript variant B (CG17836), mRNA
0	0	3	4	7	NM_164472.1	CG17242-RA (CG17242), mRNA
0	0	3	3	4	NM_169693.1	CG4261-RA (Hel89B), mRNA
0	0	2	3	3	NM_140569.2	CG5841-RA (mib1), mRNA
0	0	2	3	2	NM_206627.1	CG4068-RB, transcript variant B (CG4068), mRNA
0	0	2	3	2	NM_140888.2	CG8743-RA (CG8743), mRNA
0	0	2	2	5	NM_134871.1	CG3131-RA (Duox), mRNA
0	0	2	2	5	NM_169548.1	CG9351-RB, transcript variant B (flfl), mRNA
0	0	2	2	5	NM_142047.1	CG9351-RA, transcript variant A (flfl), mRNA
0	0	2	2	5	NM_169549.1	CG9351-RC, transcript variant C (flfl), mRNA
0	0	1	6	2	NM_169207.1	CG7602-RB, transcript variant B (DNApol-iota), mRNA
0	0	1	6	2	NM_141515.2	CG7602-RA, transcript variant A (DNApol-iota), mRNA
0	0	1	4	5	NM_139960.1	CG7170-RA (Jon66Cii), mRNA
0	0	1	4	4	NM_142446.2	CG7142-RA (CG7142), mRNA
0	0	1	4	3	NM_168271.1	CG7118-RA (Jon66Ci), mRNA
0	0	1	3	4	NM_057232.3	CG1934-RA (ImpE2), mRNA
0	0	1	3	3	NM_138127.1	CG3650-RA (CG3650), mRNA
0	0	1	3	3	NM_079011.2	CG6692-RC, transcript variant C (Cp1), mRNA
0	0	1	3	3	NM_166027.1	CG6692-RB, transcript variant B (Cp1), mRNA
0	0	1	3	3	NM_166026.1	CG6692-RA, transcript variant A (Cp1), mRNA
0	0	1	2	6	NM_139833.1	CG8616-RA (CG8616), mRNA
0	0	1	2	3	NM_170038.1	CG31151-RB, transcript variant B (wge), mRNA
0	0	1	2	3	NM_170037.1	CG31151-RA, transcript variant A (wge), mRNA
0	0	1	2	3	NM_001043281.1	CG31151-RC, transcript variant C (wge), mRNA
0	0	1	2	3	NM_166031.1	CG8233-RA, transcript variant A (CG8233), mRNA
0	0	1	2	3	NM_137083.2	CG8233-RB, transcript variant B (CG8233), mRNA
0	0	1	2	3	NM_166032.1	CG8233-RC, transcript variant C (CG8233), mRNA
0	0	1	2	2	NM_078671.3	CG7098-RA (dik), mRNA

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