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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 17183R-4

Symbol MED30 Full Name Mediator complex subunit 30

CG No CG17183 Old CG No CG17183

Synonyms Med30, Trap25, CG17183, Med11/TRAP25, dMED20, dTRAP25, Med20, MED30

Accession No (Link to NCBI) NM_138202.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Bauke AC, Sasse S, Matzat T, Klämbt C.
A transcriptional network controlling glial development in the Drosophila visual system.
Development (2015) 142(12) 2184-93 [ PubMed ID = 26015542 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgACGGTCAAAACCAGGGTAATC

Primer Seq. 3'

aatctagaggtaccCTGCGAGATAGCCAGCATATT

Predicted Fragment Size

IR fragment full Seq

0001 ACGGTCAAAA CCAGGGTAAT CAGGGACCAT CGAGCGGCGG CGGAGGAGGC GGGGGCCCCA 
0061 ACATGATGCC GATGGGTGGA TTTGGCATGC AGCACGGCAA TATGCAGCAA ATGCACATGT 
0121 CGCCTCAACA TCAACAGCAG CAACAACAAA TGGGCATGAT GGGCGGCCCG GGTAGCATGC 
0181 AGATGAATCC CCAAGGACCT GGAGGTCCTG GNCGGCCTGA TGCCCGGCAT GTCTCCGCAG 
0241 CACCAGATGC AGCAACAGCA GCAGCAGCAG ATGATGCAGC AGCAAATGAT GGTGCCCCAA 
0301 CAAGGAGTGG GCGTCGGAGT GGGAATGGGC GGTGGTGTGG GAATGGGCGG AGGGGGAGTG 
0361 GTTCCACAGC AGCAGCAACA GCAGCCCCAA CAAAACATGC CCCAGCAGAA CATTCCCCAA 
0421 CAGCAACAGC AACAGCAACT CAATCCGGTC GCAGGAATTC CAcNCGGTGG AgNCGGGGGC 
0481 AGCAACAATA TGCTGGCTAT CTCGCAg

in silico PCR Fragment

0001 ACGGTCAAAA CCAGGGTAAT CAGGGACCAT CGAGCGGCGG CGGAGGAGGC GGGGGCCCCA 
0061 ACATGATGCC GATGGGTGGA TTTGGCATGC AGCACGGCAA TATGCAGCAA ATGCACATGT 
0121 CGCCTCAACA TCAACAGCAG CAACAACAAA TGGGCATGAT GGGCGGCCCG GGTAGCATGC 
0181 AGATGAATCC CCAAGGACCT GGAGGTCCTG G-CGGCCTGA TGCCCGGCAT GTCTCCGCAG 
0241 CACCAGATGC AGCAACAGCA GCAGCAGCAG ATGATGCAGC AGCAAATGAT GGTGCCCCAA 
0301 CAAGGAGTGG GCGTCGGAGT GGGAATGGGC GGTGGTGTGG GAATGGGCGG AGGGGGAGTG 
0361 GTTCCACAGC AGCAGCAACA GCAGCCCCAA CAAAACATGC CCCAGCAGAA CATTCCC--- 
0421 ---CAACAGC AACAGCAACT CAATCCGGTC GCAGGAATTC CACCCGGTGG AGCCGGGGGC 
0481 AGCAACAATA TGCTGGCTAT CTCGCAG

Assemble Data


17183R-4.IR_full       1   ACGGTCAAAACCAGGGTAATCAGGGACCATCGAGCGGCGGCGGAGGAGGCGGGGGCCCCA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17183R-4.in silico     1   ACGGTCAAAACCAGGGTAATCAGGGACCATCGAGCGGCGGCGGAGGAGGCGGGGGCCCCA 60


17183R-4.IR_full       61  ACATGATGCCGATGGGTGGATTTGGCATGCAGCACGGCAATATGCAGCAAATGCACATGT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17183R-4.in silico     61  ACATGATGCCGATGGGTGGATTTGGCATGCAGCACGGCAATATGCAGCAAATGCACATGT 120


17183R-4.IR_full       121 CGCCTCAACATCAACAGCAGCAACAACAAATGGGCATGATGGGCGGCCCGGGTAGCATGC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17183R-4.in silico     121 CGCCTCAACATCAACAGCAGCAACAACAAATGGGCATGATGGGCGGCCCGGGTAGCATGC 180


17183R-4.IR_full       181 AGATGAATCCCCAAGGACCTGGAGGTCCTGGNCGGCCTGATGCCCGGCATGTCTCCGCAG 240
                           ||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||
17183R-4.in silico     181 AGATGAATCCCCAAGGACCTGGAGGTCCTGG-CGGCCTGATGCCCGGCATGTCTCCGCAG 240


17183R-4.IR_full       241 CACCAGATGCAGCAACAGCAGCAGCAGCAGATGATGCAGCAGCAAATGATGGTGCCCCAA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17183R-4.in silico     241 CACCAGATGCAGCAACAGCAGCAGCAGCAGATGATGCAGCAGCAAATGATGGTGCCCCAA 300


17183R-4.IR_full       301 CAAGGAGTGGGCGTCGGAGTGGGAATGGGCGGTGGTGTGGGAATGGGCGGAGGGGGAGTG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17183R-4.in silico     301 CAAGGAGTGGGCGTCGGAGTGGGAATGGGCGGTGGTGTGGGAATGGGCGGAGGGGGAGTG 360


17183R-4.IR_full       361 GTTCCACAGCAGCAGCAACAGCAGCCCCAACAAAACATGCCCCAGCAGAACATTCCCCAA 420
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||||   
17183R-4.in silico     361 GTTCCACAGCAGCAGCAACAGCAGCCCCAACAAAACATGCCCCAGCAGAACATTCCC--- 420


17183R-4.IR_full       421 CAGCAACAGCAACAGCAACTCAATCCGGTCGCAGGAATTCCACNCGGTGGAGNCGGGGGC 480
                              |||||||||||||||||||||||||||||||||||||||| |||||||| |||||||
17183R-4.in silico     421 ---CAACAGCAACAGCAACTCAATCCGGTCGCAGGAATTCCACCCGGTGGAGCCGGGGGC 480


17183R-4.IR_full       481 AGCAACAATATGCTGGCTATCTCGCAG 507
                           |||||||||||||||||||||||||||
17183R-4.in silico     481 AGCAACAATATGCTGGCTATCTCGCAG 507

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	14	100	NM_138202.2	CG17183-RA (MED30), mRNA
6.84	33	315	858	2410	NM_167335.1	CG4013-RC, transcript variant C (Smr), mRNA
6.84	33	315	858	2410	NM_167334.1	CG4013-RB, transcript variant B (Smr), mRNA
6.84	33	315	858	2410	NM_080536.2	CG4013-RA, transcript variant A (Smr), mRNA
5.18	25	114	384	1158	NM_080376.1	CG8118-RA, transcript variant A (mam), mRNA
5.18	25	114	384	1155	NM_166030.1	CG8118-RB, transcript variant B (mam), mRNA
3.52	17	37	219	558	NM_080364.3	CG5461-RA, transcript variant A (bun), mRNA
3.52	17	37	197	476	NM_001042894.1	CG5461-RF, transcript variant F (bun), mRNA
3.31	16	44	276	734	NM_139493.2	CG2083-RA (CG2083), mRNA
2.9	14	73	243	694	NM_131975.3	CG32767-RA, transcript variant A (CG32767), mRNA
2.9	14	73	243	694	NM_206628.1	CG32767-RB, transcript variant B (CG32767), mRNA
2.9	14	51	197	488	NM_167000.1	CG32778-RA (CG32778), mRNA
2.9	14	41	130	326	NM_166446.1	CG9696-RD, transcript variant D (dom), mRNA
2.9	14	41	130	326	NM_080094.2	CG9696-RA, transcript variant A (dom), mRNA
2.69	13	26	106	283	NM_169653.2	CG31302-RB, transcript variant B (CG31302), mRNA
2.69	13	26	106	283	NM_169652.2	CG31302-RA, transcript variant A (CG31302), mRNA
2.69	13	26	106	283	NM_169654.2	CG31302-RC, transcript variant C (CG31302), mRNA
2.48	12	78	343	922	NM_079903.2	CG15319-RB (nej), mRNA
2.48	12	46	197	500	NM_176592.1	CG12071-RB, transcript variant B (CG12071), mRNA
2.48	12	46	197	500	NM_143575.2	CG12071-RA, transcript variant A (CG12071), mRNA
2.48	12	42	197	478	NM_079507.2	CG2530-RA (corto), mRNA
2.28	11	86	269	797	NM_079671.2	CG7847-RA, transcript variant A (sr), mRNA
2.28	11	53	202	514	NM_134474.4	CG32532-RA (CG32532), mRNA
2.07	10	66	245	723	NM_169782.1	CG31243-RA, transcript variant A (cpo), mRNA
2.07	10	66	245	723	NM_169781.1	CG31243-RE, transcript variant E (cpo), mRNA
2.07	10	66	245	722	NM_169783.1	CG31243-RB, transcript variant B (cpo), mRNA
2.07	10	40	186	590	NM_080105.2	CG31243-RF, transcript variant F (cpo), mRNA
2.07	10	34	159	357	NM_206456.1	CG32466-RB, transcript variant B (rn), mRNA
2.07	10	31	144	359	NM_132004.2	CG4136-RA (CG4136), mRNA
2.07	10	31	112	230	NM_057511.3	CG3936-RA (N), mRNA

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