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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
17181R-1 |
| Symbol |
CG17181 |
Full Name |
CG17181 |
| CG No |
CG17181 |
Old CG No |
CG17181 |
| Synonyms |
CG17181 |
| Accession No (Link to NCBI) |
NM_138196.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.<br> Zyxin links fat signaling to the hippo pathway.<br> PLoS Biol (2011) 9(6) e1000624
[
PubMed ID = 21666802
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATGACGAAGGACCAGCAGGCG |
| Primer Seq. 3' |
aatctagaggtaccATCGAGTAGGCGGGCATCGAA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ATGACGAAGG ACCAGCAGGC GACACCCATT CCCGAGGAGT TGTACAATCT CACCCAGCTG
0061 GCCGAGGTTA CTCTGTCCGT TGGTCCTCTG GTCACGGACG AGGTGAAGCC ACTNCCACTG
0121 TACGCCTCCT CGGACGACGA CTCCAACTAC TACAGCCACA AGGTCTTCGA TCGCAGGAAA
0181 CTGCGTCGTT GCACAATCTC CGACTCGAAT NTCCTGCGCC AGCNAGCANT TCCAGTTCCA
0241 CCTCCAGCCG TCAATCCTCG GAGGACCACC CTGGGACTCC CAGGGGCACT CATCGGTACA
0301 CCAGCATCAT GGGGAGCAGG GTGAAATCCT CAACAGCACT TCGCTGCTGG AGGACGAGCA
0361 CATCTGTCCC GAGTGCGGAA AGAAATACTC CACATCCTCC AACCTAGCAC GTCATAGACA
0421 AACGCACAGA TCAATCATGG ATAAGAAAGC GCGTCACTGT CCTTATTGCG AGAAGGTGTA
0481 CGTTTTCGAT GCCCGCCTAC TCGAT |
| in silico PCR Fragment |
0001 ATGACGAAGG ACCAGCAGGC GACACCCATT CCCGAGGAGT TGTACAATCT CACCCAGCTG
0061 GCCGAGGTTA CTCTGTCCGT TGGTCCTCTG GTCACGGACG AGGTGAAGCC ACTGCCACTG
0121 TACGCCTCCT CGGACGACGA CTCCAACTAC TACAGCCACA AGGTCTTCGA TCGCAGGAAA
0181 CTGCGTCGTT GCACAATCTC CGACTCGAAT -TCCTGCGCC AGC-AGCAGT TCCAGTTCCA
0241 CCTCCAGCCG TCAATCCTCG GAGGACCA-C CTGGGACT-C CAGGGGCACT CATCGGTGCA
0301 CCACCATCAT GGGGAGCAGG GCGAAATCCT CAACAGCACT TCGTTGCTGG AGGACGAGCA
0361 CATCTGTCCC GAGTGCGGAA AGAAATACTC CACATCCTCC AACCTAGCAC GTCATAGACA
0421 AACGCACAGA TCAATCATGG ATAAGAAAGC GCGTCACTGT CCTTATTGCG AGAAGGTGTA
0481 CG-TTTCGAT GCCCGCCTAC TCGAT |
| Assemble Data |
<PRE>
17181R-1.IR_full 1 ATGACGAAGGACCAGCAGGCGACACCCATTCCCGAGGAGTTGTACAATCTCACCCAGCTG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17181R-1.in silico 1 ATGACGAAGGACCAGCAGGCGACACCCATTCCCGAGGAGTTGTACAATCTCACCCAGCTG 60
17181R-1.IR_full 61 GCCGAGGTTACTCTGTCCGTTGGTCCTCTGGTCACGGACGAGGTGAAGCCACT<span class="snp"><tt>N</tt></span>CCACTG 120
|||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||
17181R-1.in silico 61 GCCGAGGTTACTCTGTCCGTTGGTCCTCTGGTCACGGACGAGGTGAAGCCACT<span class="snp"><tt>G</tt></span>CCACTG 120
17181R-1.IR_full 121 TACGCCTCCTCGGACGACGACTCCAACTACTACAGCCACAAGGTCTTCGATCGCAGGAAA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17181R-1.in silico 121 TACGCCTCCTCGGACGACGACTCCAACTACTACAGCCACAAGGTCTTCGATCGCAGGAAA 180
17181R-1.IR_full 181 CTGCGTCGTTGCACAATCTCCGACTCGAAT<span class="snp"><tt>N</tt></span>TCCTGCGCCAGC<span class="snp"><tt>N</tt></span>AGCA<span class="snp"><tt>N</tt></span>TTCCAGTTCCA 240
||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||<span class="snp"><tt> </tt></span>||||<span class="snp"><tt> </tt></span>|||||||||||
17181R-1.in silico 181 CTGCGTCGTTGCACAATCTCCGACTCGAAT<span class="snp"><tt>-</tt></span>TCCTGCGCCAGC<span class="snp"><tt>-</tt></span>AGCA<span class="snp"><tt>G</tt></span>TTCCAGTTCCA 240
17181R-1.IR_full 241 CCTCCAGCCGTCAATCCTCGGAGGACCA<span class="snp"><tt>C</tt></span>CCTGGGACT<span class="snp"><tt>C</tt></span>CCAGGGGCACTCATCGGT<span class="snp"><tt>A</tt></span>CA 300
||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||<span class="snp"><tt> </tt></span>||
17181R-1.in silico 241 CCTCCAGCCGTCAATCCTCGGAGGACCA<span class="snp"><tt>-</tt></span>CCTGGGACT<span class="snp"><tt>-</tt></span>CCAGGGGCACTCATCGGT<span class="snp"><tt>G</tt></span>CA 300
17181R-1.IR_full 301 CCA<span class="snp"><tt>G</tt></span>CATCATGGGGAGCAGGG<span class="snp"><tt>T</tt></span>GAAATCCTCAACAGCACTTCG<span class="snp"><tt>C</tt></span>TGCTGGAGGACGAGCA 360
|||<span class="snp"><tt> </tt></span>|||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||
17181R-1.in silico 301 CCA<span class="snp"><tt>C</tt></span>CATCATGGGGAGCAGGG<span class="snp"><tt>C</tt></span>GAAATCCTCAACAGCACTTCG<span class="snp"><tt>T</tt></span>TGCTGGAGGACGAGCA 360
17181R-1.IR_full 361 CATCTGTCCCGAGTGCGGAAAGAAATACTCCACATCCTCCAACCTAGCACGTCATAGACA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17181R-1.in silico 361 CATCTGTCCCGAGTGCGGAAAGAAATACTCCACATCCTCCAACCTAGCACGTCATAGACA 420
17181R-1.IR_full 421 AACGCACAGATCAATCATGGATAAGAAAGCGCGTCACTGTCCTTATTGCGAGAAGGTGTA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17181R-1.in silico 421 AACGCACAGATCAATCATGGATAAGAAAGCGCGTCACTGTCCTTATTGCGAGAAGGTGTA 480
17181R-1.IR_full 481 CG<span class="snp"><tt>T</tt></span>TTTCGATGCCCGCCTACTCGAT 505
||<span class="snp"><tt> </tt></span>||||||||||||||||||||||
17181R-1.in silico 481 CG<span class="snp"><tt>-</tt></span>TTTCGATGCCCGCCTACTCGAT 505
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_138196.1 |
CG17181-RA (CG17181), mRNA |
| 0.2 |
1 |
4 |
5 |
4 |
NM_079322.2 |
CG10605-RA (caup), mRNA |
| 0 |
0 |
3 |
2 |
35 |
NM_131995.1 |
CG15784-RA (CG15784), mRNA |
| 0 |
0 |
3 |
2 |
6 |
NM_176739.1 |
CG33173-RA (CG33173), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_078612.2 |
CG8470-RA (mRpS30), mRNA |
| 0 |
0 |
2 |
6 |
14 |
NM_079735.3 |
CG4637-RA, transcript variant A (hh), mRNA |
| 0 |
0 |
2 |
6 |
14 |
NM_001038976.1 |
CG4637-RB, transcript variant B (hh), mRNA |
| 0 |
0 |
2 |
4 |
12 |
NM_133144.2 |
CG7893-RA, transcript variant A (vav), mRNA |
| 0 |
0 |
2 |
4 |
12 |
NM_167642.1 |
CG7893-RB, transcript variant B (vav), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_169036.1 |
CG31543-RC, transcript variant C (Hph), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_141268.2 |
CG31543-RA, transcript variant A (Hph), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_169037.1 |
CG31543-RB, transcript variant B (Hph), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_134518.2 |
CG11943-RA, transcript variant A (CG11943), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_167687.1 |
CG11943-RB, transcript variant B (CG11943), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_137546.2 |
CG15105-RA, transcript variant A (CG15105), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_166318.1 |
CG15105-RB, transcript variant B (CG15105), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_078937.3 |
CG2411-RA (ptc), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_137864.1 |
CG30259-RA (CG30259), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_142397.1 |
CG14318-RA (CG14318), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_001042883.1 |
CG18405-RC, transcript variant C (Sema-1a), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_079918.2 |
CG7919-RA (fan), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_137958.2 |
CG3906-RA (CG3906), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_141047.2 |
CG7338-RA (CG7338), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_176504.1 |
CG33207-RB, transcript variant B (pxb), mRNA |
| 0 |
0 |
0 |
8 |
9 |
NM_169826.1 |
CG31224-RA (CG31224), mRNA |
| 0 |
0 |
0 |
7 |
8 |
NM_143378.2 |
CG1523-RA (CG1523), mRNA |
| 0 |
0 |
0 |
7 |
4 |
NM_134854.2 |
CG17301-RA (CG17301), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_168808.1 |
CG8756-RC, transcript variant C (LCBP1), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_168809.1 |
CG8756-RA, transcript variant A (LCBP1), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_140885.1 |
CG8756-RD, transcript variant D (LCBP1), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| +- |
|
| DB |
 |
| ++ |
|
| DC |
 |
| ++ |
|
| DM |
 |
| + |
|
| VM |
 |
| + |
|
| VB |
 |
| |
|
|
Caspase-3 signal
| N |
|
| + |
|
| DB |
|
| ++ |
|
| DC |
|
| ++ |
|
| DM |
|
| ++ |
|
| VM |
|
| + |
|
| VB |
|
| |
|
|
| Dcompartment |
|
Penetrance |
| + |
|
|
|
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
|
|
|
