NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

17028R-3

Symbol

CG17028

Full Name

CG17028

CG No

CG17028

Old CG No

CG17028

Synonyms

IMP, CG17028

Accession No (Link to NCBI)

NM_140565.1

Inserted Chr.

lll

Insertional Mutation

2 lethal

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGATGCCCCCACTTGGATCATA

Primer Seq. 3'

aatctagaggtaccCAGTCGGGTTTCATCACATCG

Predicted Fragment Size

IR fragment full Seq

0001 GATGCCCCCA CTTGGATCAT AGATCCCATT GATGGCACCA ACAACTACGT TCGAAAGATC 
0061 CCCCATTGCT GTATTTCCGT GGGCTTGGCC ATAAACAAAG AGCTGGTGCT GGGGATTGTG 
0121 TACAATCCAT CTGCAAATGA GTTGTATTCA GCTTGGCAAG GCCACGGAGC TTATTTAAAT 
0181 GGACAACCCA TTGAGGTTTC CAACGCTAAG AAGATAAACC AAGCATTGGT GTGCTATGAG 
0241 ATCTCCCTAA TCGTGGTGTC TAAGGGGCGG GACAAGAACG TGAAGCGTCT CTACAAGCTG 
0301 GCCTCGAGTG CCACTGGCAC CCGCAGCTTT GGATNNGCAG CTCTCaNNNT CTGCTATNAT 
0361 AgCCGCCGGC AGATGTGACG CCTATCATGT GGAGAATCTA AAGCCCTGGG ATCTGGCTGG 
0421 CGGTGCGGTA ATCCTCAGGG AAgCCGGTGG CAGGGTATAT CACACAANCG GTGCAAGATT 
0481 CGATGTGATG AAAcCCGACT G

in silico PCR Fragment

0001 GATGCCCCCA CTTGGATCAT AGATCCCATT GATGGCACCA ACAACTACGT TCGAAAGATC 
0061 CCCCATTGCT GTATTTCCGT GGGCTTGGCC ATAAACAAAG AGCTGGTGCT GGGGATTGTG 
0121 TACAATCCAT CTGCAAATGA GTTGTATTCA GCTTGGCAAG GCCACGGAGC TTATTTAAAT 
0181 GGACAACCCA TTGAGGTTTC CAACGCTAAG AAGATAAACC AAGCATTGGT GTGCTATGAG 
0241 ATCTCCCTAA TCGTGGTGTC TAAGGGGCGG GACAAGAACG TGAAGCGTCT CTACAAGCTG 
0301 GCCTCGAGTG CCACTGGCAC CCGCAGCTTT GGATGCGCAG CTCTCACGCT CTGCTAT-AT 
0361 AGCCGCCGGC AGATGTGACG CCTATCATGT GGAGAATCTA AAGCCCTGGG ATCTGGCTGG 
0421 CGGTGCGGTA ATCCTCAGGG AAGCCGGTGG CAGGGTATAT CACACAAGCG GTGCAAGATT 
0481 CGATGTGATG AAACCCGACT G

Assemble Data

<PRE>

17028R-3.IR_full       1   GATGCCCCCACTTGGATCATAGATCCCATTGATGGCACCAACAACTACGTTCGAAAGATC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17028R-3.in silico     1   GATGCCCCCACTTGGATCATAGATCCCATTGATGGCACCAACAACTACGTTCGAAAGATC 60


17028R-3.IR_full       61  CCCCATTGCTGTATTTCCGTGGGCTTGGCCATAAACAAAGAGCTGGTGCTGGGGATTGTG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17028R-3.in silico     61  CCCCATTGCTGTATTTCCGTGGGCTTGGCCATAAACAAAGAGCTGGTGCTGGGGATTGTG 120


17028R-3.IR_full       121 TACAATCCATCTGCAAATGAGTTGTATTCAGCTTGGCAAGGCCACGGAGCTTATTTAAAT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17028R-3.in silico     121 TACAATCCATCTGCAAATGAGTTGTATTCAGCTTGGCAAGGCCACGGAGCTTATTTAAAT 180


17028R-3.IR_full       181 GGACAACCCATTGAGGTTTCCAACGCTAAGAAGATAAACCAAGCATTGGTGTGCTATGAG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17028R-3.in silico     181 GGACAACCCATTGAGGTTTCCAACGCTAAGAAGATAAACCAAGCATTGGTGTGCTATGAG 240


17028R-3.IR_full       241 ATCTCCCTAATCGTGGTGTCTAAGGGGCGGGACAAGAACGTGAAGCGTCTCTACAAGCTG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17028R-3.in silico     241 ATCTCCCTAATCGTGGTGTCTAAGGGGCGGGACAAGAACGTGAAGCGTCTCTACAAGCTG 300


17028R-3.IR_full       301 GCCTCGAGTGCCACTGGCACCCGCAGCTTTGGAT<span class="snp"><tt>N</tt></span><span class="snp"><tt>N</tt></span>GCAGCTCTCA<span class="snp"><tt>N</tt></span><span class="snp"><tt>N</tt></span><span class="snp"><tt>N</tt></span>TCTGCTAT<span class="snp"><tt>N</tt></span>AT 360
                           ||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>||||||||||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>||||||||<span class="snp"><tt>&nbsp;</tt></span>||
17028R-3.in silico     301 GCCTCGAGTGCCACTGGCACCCGCAGCTTTGGAT<span class="snp"><tt>G</tt></span><span class="snp"><tt>C</tt></span>GCAGCTCTCA<span class="snp"><tt>C</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>C</tt></span>TCTGCTAT<span class="snp"><tt>-</tt></span>AT 360


17028R-3.IR_full       361 AGCCGCCGGCAGATGTGACGCCTATCATGTGGAGAATCTAAAGCCCTGGGATCTGGCTGG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17028R-3.in silico     361 AGCCGCCGGCAGATGTGACGCCTATCATGTGGAGAATCTAAAGCCCTGGGATCTGGCTGG 420


17028R-3.IR_full       421 CGGTGCGGTAATCCTCAGGGAAGCCGGTGGCAGGGTATATCACACAA<span class="snp"><tt>N</tt></span>CGGTGCAAGATT 480
                           |||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||
17028R-3.in silico     421 CGGTGCGGTAATCCTCAGGGAAGCCGGTGGCAGGGTATATCACACAA<span class="snp"><tt>G</tt></span>CGGTGCAAGATT 480


17028R-3.IR_full       481 CGATGTGATGAAACCCGACTG 501
                           |||||||||||||||||||||
17028R-3.in silico     481 CGATGTGATGAAACCCGACTG 501

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	1	NM_140565.1	CG17028-RA (CG17028), mRNA
0.41	2	9	10	1	NM_140563.1	CG17026-RA (CG17026), mRNA
0.2	1	4	11	10	NM_141038.2	CG9389-RA (CG9389), mRNA
0.2	1	2	4	4	NM_167095.1	CG32743-RA (Smg1), mRNA
0	0	15	53	58	NM_140564.1	CG17029-RA (CG17029), mRNA
0	0	10	9	2	NM_140566.2	CG17027-RA (CG17027), mRNA
0	0	4	3	2	NM_167259.1	CG1615-RA, transcript variant A (Ork1), mRNA
0	0	4	3	2	NM_078557.4	CG1615-RB, transcript variant B (Ork1), mRNA
0	0	3	5	2	NM_142222.1	CG5302-RA (CG5302), mRNA
0	0	2	4	5	NM_137268.1	CG30099-RA (CG30099), mRNA
0	0	1	2	3	NM_140803.1	CG14073-RB, transcript variant B (CG14073), mRNA
0	0	0	6	6	NM_168054.1	CG32259-RB (CG32259), mRNA
0	0	0	6	4	NM_169776.1	CG7660-RA, transcript variant A (pxt), mRNA
0	0	0	6	4	NM_142391.3	CG7660-RB, transcript variant B (pxt), mRNA
0	0	0	5	6	NM_164580.1	CG2969-RB, transcript variant B (Atet), mRNA
0	0	0	5	6	NM_078747.2	CG2969-RA, transcript variant A (Atet), mRNA
0	0	0	4	5	NM_164605.1	CG8886-RB, transcript variant B (l(2)05714), mRNA
0	0	0	4	5	NM_143776.3	CG8886-RA, transcript variant A (l(2)05714), mRNA
0	0	0	4	3	NM_170435.1	CG31009-RA (Cad99C), mRNA
0	0	0	3	8	NM_168893.2	CG9391-RB, transcript variant B (CG9391), mRNA
0	0	0	3	8	NM_141037.3	CG9391-RA, transcript variant A (CG9391), mRNA
0	0	0	3	8	NM_165145.1	CG4824-RD, transcript variant D (BicC), mRNA
0	0	0	3	8	NM_165144.1	CG4824-RB, transcript variant B (BicC), mRNA
0	0	0	3	8	NM_057517.2	CG4824-RA, transcript variant A (BicC), mRNA
0	0	0	3	6	NM_079122.2	CG4545-RA (SerT), mRNA
0	0	0	3	6	NM_142180.2	CG4285-RA (CG4285), mRNA
0	0	0	3	5	NM_139512.1	CG14955-RA, transcript variant A (CG14955), mRNA
0	0	0	3	5	NM_167985.1	CG14955-RB, transcript variant B (CG14955), mRNA
0	0	0	3	5	NM_079854.1	CG9688-RA (mRpS18C), mRNA
0	0	0	3	3	NM_142457.2	CG7669-RA (CG7669), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

dme00562 : Inositol phosphate metabolism
dme01100 : Metabolic pathways
dme04070 : Phosphatidylinositol signaling system

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