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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

16980R-3

Symbol

RhoGAP71E

Full Name

RhoGAP71E

CG No

CG32149

Old CG No

CG16980

Synonyms

CG16980, CG7396, RhoGAP-71E1, l(3)j6B9, CG32149, BcDNA:LD07615, RhoGAP71E

Accession No (Link to NCBI)

NM_140527.2

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

pupal lethal

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATGTCCAGCTGGGCAGAACGC

Primer Seq. 3'

aatctagaggtaccACCTGTGCAGTCGCTCCGTTT

Predicted Fragment Size

IR fragment full Seq

0001 ATGTCCAGCT GGGCAGAACG CGTCCATNAG GcGCGCCGCG GATTTGGGCA ACGTGGTGAC 
0061 CTCCTGCGGC CACCCAGCCA CCGCTCCCGC CTACCCGTAC CGCCTCGAAA AGGTCAAATT 
0121 CGGTGTGCCG CTGGAGGAGG TGTGCAAGCA CAACAACAAC ATTCCCGGGC CCCTGCTCGT 
0181 CCTGATCCTC AAACTGAACA AGGAGTCACC GAACCGCCGC GATGTATTCC GCGCACCCGG 
0241 CCACCAGGGC GCAATGAAGA AGCTCATCCA CTTCCTGCAG GCCGGTCGGC TCGTCAACGT 
0301 GGACAACTAT TCGGTGTTCA CCATCGCCAG CGTGCTCAAG AAGTTCCTGC GCAAGATCCC 
0361 CAATGGGATA TTCGGGCGCA GCGGCGAGAA TGGAGCTGTT CGCCATCAAT GATCTGCAAA 
0421 ACGAAGCCGA GCAAACGGAG CGACTGCACA GAC

in silico PCR Fragment

0001 ATGTCCAGCT GGGCAGAACG CGTCCAT-AG GCGCGCCGCG GATTTGGGCA ACGTGGTGAC 
0061 CTCCTGCGGC CACCCAGCCA CCGCTCCCGC CTACCCGTAC CGCCTCGAAA AGGTCAAATT 
0121 CGGTGTGCCG CTGGAGGAGG TGTGCAAGCA CAACAACAAC ATTCCCGGGC CCCTGCTCGT 
0181 CCTGATCCTC AAACTGAACA AGGAGTCACC GAACCGCCGC GATGTATTCC GCGCACCCGG 
0241 CCACCAGGGC GCAATGAAGA AGCTCATCCA CTTCCTGCAG GCCGGTCGGC TCGTCAACGT 
0301 GGACAACTAT TCGGTGTTCA CCATCGCCAG CGTGCTCAAG AAGTTCCTGC GCAAGATCCC 
0361 CAATGGGATA TTCGGGCGCA GCGGCGAG-A TGGAGCTGTT CGCCATCAAT GATCTGCAAA 
0421 ACGAAGCCGA GCAAACGGAG CGACTGCACA GAC

Assemble Data

<PRE>

16980R-3.IR_full       1   ATGTCCAGCTGGGCAGAACGCGTCCAT<span class="snp"><tt>N</tt></span>AGGCGCGCCGCGGATTTGGGCAACGTGGTGAC 60
                           |||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||
16980R-3.in silico     1   ATGTCCAGCTGGGCAGAACGCGTCCAT<span class="snp"><tt>-</tt></span>AGGCGCGCCGCGGATTTGGGCAACGTGGTGAC 60


16980R-3.IR_full       61  CTCCTGCGGCCACCCAGCCACCGCTCCCGCCTACCCGTACCGCCTCGAAAAGGTCAAATT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16980R-3.in silico     61  CTCCTGCGGCCACCCAGCCACCGCTCCCGCCTACCCGTACCGCCTCGAAAAGGTCAAATT 120


16980R-3.IR_full       121 CGGTGTGCCGCTGGAGGAGGTGTGCAAGCACAACAACAACATTCCCGGGCCCCTGCTCGT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16980R-3.in silico     121 CGGTGTGCCGCTGGAGGAGGTGTGCAAGCACAACAACAACATTCCCGGGCCCCTGCTCGT 180


16980R-3.IR_full       181 CCTGATCCTCAAACTGAACAAGGAGTCACCGAACCGCCGCGATGTATTCCGCGCACCCGG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16980R-3.in silico     181 CCTGATCCTCAAACTGAACAAGGAGTCACCGAACCGCCGCGATGTATTCCGCGCACCCGG 240


16980R-3.IR_full       241 CCACCAGGGCGCAATGAAGAAGCTCATCCACTTCCTGCAGGCCGGTCGGCTCGTCAACGT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16980R-3.in silico     241 CCACCAGGGCGCAATGAAGAAGCTCATCCACTTCCTGCAGGCCGGTCGGCTCGTCAACGT 300


16980R-3.IR_full       301 GGACAACTATTCGGTGTTCACCATCGCCAGCGTGCTCAAGAAGTTCCTGCGCAAGATCCC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16980R-3.in silico     301 GGACAACTATTCGGTGTTCACCATCGCCAGCGTGCTCAAGAAGTTCCTGCGCAAGATCCC 360


16980R-3.IR_full       361 CAATGGGATATTCGGGCGCAGCGGCGAG<span class="snp"><tt>A</tt></span>ATGGAGCTGTTCGCCATCAATGATCTGCAAA 420
                           ||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||
16980R-3.in silico     361 CAATGGGATATTCGGGCGCAGCGGCGAG<span class="snp"><tt>-</tt></span>ATGGAGCTGTTCGCCATCAATGATCTGCAAA 420


16980R-3.IR_full       421 ACGAAGCCGAGCAAACGGAGCGACTGCACAGAC 453
                           |||||||||||||||||||||||||||||||||
16980R-3.in silico     421 ACGAAGCCGAGCAAACGGAGCGACTGCACAGAC 453

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	433	0	0	0	NM_168629.2	CG32149-RC, transcript variant C (RhoGAP71E), mRNA
100	433	0	0	0	NM_140527.2	CG32149-RA, transcript variant A (RhoGAP71E), mRNA
100	433	0	0	0	NM_168630.1	CG32149-RB, transcript variant B (RhoGAP71E), mRNA
0.46	2	3	3	4	NM_132239.1	CG1632-RA (CG1632), mRNA
0	0	3	3	2	NM_137231.2	CG8388-RA (CG8388), mRNA
0	0	3	2	3	NM_142524.2	CG6040-RA (CG6040), mRNA
0	0	2	3	7	NM_001043070.1	CG34146-RA (brp), mRNA
0	0	2	3	3	NM_136501.1	CG11191-RA (CG11191), mRNA
0	0	2	2	2	NM_166641.1	CG2987-RB, transcript variant B (alpha-catenin-related), mRNA
0	0	2	2	2	NM_143816.2	CG2987-RA, transcript variant A (alpha-catenin-related), mRNA
0	0	1	4	6	NM_143262.1	CG14254-RA (CG14254), mRNA
0	0	1	3	4	NM_057454.3	CG16720-RA, transcript variant A (5-HT1A), mRNA
0	0	1	3	4	NM_166322.1	CG16720-RB, transcript variant B (5-HT1A), mRNA
0	0	1	3	2	NM_057594.3	CG5848-RB, transcript variant B (cact), mRNA
0	0	1	3	2	NM_205999.1	CG5848-RD, transcript variant D (cact), mRNA
0	0	1	3	2	NM_057595.3	CG5848-RC, transcript variant C (cact), mRNA
0	0	1	3	2	NM_165152.1	CG5848-RA, transcript variant A (cact), mRNA
0	0	1	2	10	NM_143108.1	CG11859-RA (CG11859), mRNA
0	0	1	2	9	NM_138049.2	CG3328-RA (CG3328), mRNA
0	0	1	2	6	NM_137794.2	CG11073-RA (CG11073), mRNA
0	0	1	2	5	NM_164406.1	CG5080-RA, transcript variant A (CG5080), mRNA
0	0	1	2	5	NM_134734.2	CG5080-RB, transcript variant B (CG5080), mRNA
0	0	1	2	4	NM_142098.1	CG8538-RA (CG8538), mRNA
0	0	1	2	3	NM_170213.2	CG31102-RA (CG31102), mRNA
0	0	1	2	2	NM_079649.2	CG18740-RA (mor), mRNA
0	0	1	2	2	NM_137311.2	CG8566-RD (unc-104), mRNA
0	0	0	7	3	NM_079222.2	CG8339-RA (sfl), mRNA
0	0	0	5	4	NM_170361.1	CG5514-RA, transcript variant A (CG5514), mRNA
0	0	0	5	4	NM_143342.1	CG5514-RB, transcript variant B (CG5514), mRNA
0	0	0	5	3	NM_080335.2	CG2984-RA (Pp2C1), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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