National Institute of Genetics
Contact us      Quick Search       
NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -
Home Home
Japanese | English  
NIG-RNAi Strains Detail
Request : Click the "Order" button to request for this stock   request request_help.gif
Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 16980R-1 
 Symbol RhoGAP71E  Full Name RhoGAP71E 
 CG No CG32149  Old CG No CG16980 
 Synonyms CG16980, CG7396, RhoGAP-71E1, l(3)j6B9, CG32149, BcDNA:LD07615, RhoGAP71E 
 Accession No (Link to NCBI) NM_140527.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees pupal lethal 
 Map Viewer
 Reference
[Please submit your publication]
 
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgATGTCCAGCTGGGCAGAACGC 
 Primer Seq. 3'
aatctagaggtaccACCTGTGCAGTCGCTCCGTTT 
 Predicted Fragment Size
451 
 IR fragment full Seq
0001 ATGTCCAGCT GGGCAGAACG CGTCCATNAG GcGCGCCGCG GATTTGGGCA ACGTGGTGAC 
0061 CTCCTGCGGC CACCCAGCCA CCGCTCCCGC CTACCCGTAC CGCCTCGAAA AGGTCAAATT 
0121 CGGTGTGCCG CTGGAGGAGG TGTGCAAGCA CAACAACAAC ATTCCCGGGC CCCTGCTCGT 
0181 CCTGATCCTC AAACTGAACA AGGAGTCACC GAACCGCCGC GATGTATTCC GCGCACCCGG 
0241 CCACCAGGGC GCAATGAAGA AGCTCATCCA CTTCCTGCAG GCCGGTCGGC TCGTCAACGT 
0301 GGACAACTAT TCGGTGTTCA CCATCGCCAG CGTGCTCAAG AAGTTCCTGC GCAAGATCCC 
0361 CAATGGGATA TTCGGGCGCA GCGGCGAGAA TGGAGCTGTT CGCCATCAAT GATCTGCAAA 
0421 ACGAAGCCGA GCAAACGGAG CGACTGCACA GAC 
 in silico PCR Fragment
0001 ATGTCCAGCT GGGCAGAACG CGTCCAT-AG GCGCGCCGCG GATTTGGGCA ACGTGGTGAC 
0061 CTCCTGCGGC CACCCAGCCA CCGCTCCCGC CTACCCGTAC CGCCTCGAAA AGGTCAAATT 
0121 CGGTGTGCCG CTGGAGGAGG TGTGCAAGCA CAACAACAAC ATTCCCGGGC CCCTGCTCGT 
0181 CCTGATCCTC AAACTGAACA AGGAGTCACC GAACCGCCGC GATGTATTCC GCGCACCCGG 
0241 CCACCAGGGC GCAATGAAGA AGCTCATCCA CTTCCTGCAG GCCGGTCGGC TCGTCAACGT 
0301 GGACAACTAT TCGGTGTTCA CCATCGCCAG CGTGCTCAAG AAGTTCCTGC GCAAGATCCC 
0361 CAATGGGATA TTCGGGCGCA GCGGCGAG-A TGGAGCTGTT CGCCATCAAT GATCTGCAAA 
0421 ACGAAGCCGA GCAAACGGAG CGACTGCACA GAC 
 Assemble Data
<PRE>

16980R-1.IR_full       1   ATGTCCAGCTGGGCAGAACGCGTCCAT<span class="snp"><tt>N</tt></span>AGGCGCGCCGCGGATTTGGGCAACGTGGTGAC 60
                           |||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||
16980R-1.in silico     1   ATGTCCAGCTGGGCAGAACGCGTCCAT<span class="snp"><tt>-</tt></span>AGGCGCGCCGCGGATTTGGGCAACGTGGTGAC 60


16980R-1.IR_full       61  CTCCTGCGGCCACCCAGCCACCGCTCCCGCCTACCCGTACCGCCTCGAAAAGGTCAAATT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16980R-1.in silico     61  CTCCTGCGGCCACCCAGCCACCGCTCCCGCCTACCCGTACCGCCTCGAAAAGGTCAAATT 120


16980R-1.IR_full       121 CGGTGTGCCGCTGGAGGAGGTGTGCAAGCACAACAACAACATTCCCGGGCCCCTGCTCGT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16980R-1.in silico     121 CGGTGTGCCGCTGGAGGAGGTGTGCAAGCACAACAACAACATTCCCGGGCCCCTGCTCGT 180


16980R-1.IR_full       181 CCTGATCCTCAAACTGAACAAGGAGTCACCGAACCGCCGCGATGTATTCCGCGCACCCGG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16980R-1.in silico     181 CCTGATCCTCAAACTGAACAAGGAGTCACCGAACCGCCGCGATGTATTCCGCGCACCCGG 240


16980R-1.IR_full       241 CCACCAGGGCGCAATGAAGAAGCTCATCCACTTCCTGCAGGCCGGTCGGCTCGTCAACGT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16980R-1.in silico     241 CCACCAGGGCGCAATGAAGAAGCTCATCCACTTCCTGCAGGCCGGTCGGCTCGTCAACGT 300


16980R-1.IR_full       301 GGACAACTATTCGGTGTTCACCATCGCCAGCGTGCTCAAGAAGTTCCTGCGCAAGATCCC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16980R-1.in silico     301 GGACAACTATTCGGTGTTCACCATCGCCAGCGTGCTCAAGAAGTTCCTGCGCAAGATCCC 360


16980R-1.IR_full       361 CAATGGGATATTCGGGCGCAGCGGCGAG<span class="snp"><tt>A</tt></span>ATGGAGCTGTTCGCCATCAATGATCTGCAAA 420
                           ||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||
16980R-1.in silico     361 CAATGGGATATTCGGGCGCAGCGGCGAG<span class="snp"><tt>-</tt></span>ATGGAGCTGTTCGCCATCAATGATCTGCAAA 420


16980R-1.IR_full       421 ACGAAGCCGAGCAAACGGAGCGACTGCACAGAC 453
                           |||||||||||||||||||||||||||||||||
16980R-1.in silico     421 ACGAAGCCGAGCAAACGGAGCGACTGCACAGAC 453

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   433  NM_168629.2  CG32149-RC, transcript variant C (RhoGAP71E), mRNA 
100   433  NM_140527.2  CG32149-RA, transcript variant A (RhoGAP71E), mRNA 
100   433  NM_168630.1  CG32149-RB, transcript variant B (RhoGAP71E), mRNA 
0.46   NM_132239.1  CG1632-RA (CG1632), mRNA 
0   NM_137231.2  CG8388-RA (CG8388), mRNA 
0   NM_142524.2  CG6040-RA (CG6040), mRNA 
0   NM_001043070.1  CG34146-RA (brp), mRNA 
0   NM_136501.1  CG11191-RA (CG11191), mRNA 
0   NM_166641.1  CG2987-RB, transcript variant B (alpha-catenin-related), mRNA 
0   NM_143816.2  CG2987-RA, transcript variant A (alpha-catenin-related), mRNA 
0   NM_143262.1  CG14254-RA (CG14254), mRNA 
0   NM_057454.3  CG16720-RA, transcript variant A (5-HT1A), mRNA 
0   NM_166322.1  CG16720-RB, transcript variant B (5-HT1A), mRNA 
0   NM_057594.3  CG5848-RB, transcript variant B (cact), mRNA 
0   NM_205999.1  CG5848-RD, transcript variant D (cact), mRNA 
0   NM_057595.3  CG5848-RC, transcript variant C (cact), mRNA 
0   NM_165152.1  CG5848-RA, transcript variant A (cact), mRNA 
0   10  NM_143108.1  CG11859-RA (CG11859), mRNA 
0   NM_138049.2  CG3328-RA (CG3328), mRNA 
0   NM_137794.2  CG11073-RA (CG11073), mRNA 
0   NM_164406.1  CG5080-RA, transcript variant A (CG5080), mRNA 
0   NM_134734.2  CG5080-RB, transcript variant B (CG5080), mRNA 
0   NM_142098.1  CG8538-RA (CG8538), mRNA 
0   NM_170213.2  CG31102-RA (CG31102), mRNA 
0   NM_079649.2  CG18740-RA (mor), mRNA 
0   NM_137311.2  CG8566-RD (unc-104), mRNA 
0   NM_079222.2  CG8339-RA (sfl), mRNA 
0   NM_170361.1  CG5514-RA, transcript variant A (CG5514), mRNA 
0   NM_143342.1  CG5514-RB, transcript variant B (CG5514), mRNA 
0   NM_080335.2  CG2984-RA (Pp2C1), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway

SHIGEN - SHared Information of GENetic resources - National Institute of Genetics (NIG)
Copyright (c) National Institute of Genetics (NIG)
All rights reserved.
The NIG-Fly is a part of the National BioResource Project which started in 2002.