NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

16717R-2

Symbol

CG16717

Full Name

CG16717

CG No

CG16717

Old CG No

CG16717

Synonyms

CG16717

Accession No (Link to NCBI)

NM_140086.1

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

semi-lethal

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGTGCCAGTACATCCACTTAGC

Primer Seq. 3'

aatctagaggtaccTCATCCTCCAAATACCGACAG

Predicted Fragment Size

IR fragment full Seq

0001 NTGCCAGTAC ATCCACTTAG CCAGGATCCC ACGGCCGCTT GGAGGGAGAT TAGCAAAACG 
0061 CAGCGNGtGN TCAAGGTGAC AATGAAGCCA CCGACCACCA CGGTgGCGCC CAACAAGGCA 
0121 CGAGTGGTTT GCATGTCCGA CACGCACTCC CTGACGCCCT ACATCAAGTT CGATATACCA 
0181 GATGGCGATA TATTCATACA TGCCGGCGAT TTTACCAAGT GCGGCCAACT GGAGGAGGTG 
0241 GAGGAGTTTA ACACCTGGAT TGGAGCACTG CCCCATCGCC ACAAGATCGT GATTGCTGGC 
0301 AATCATGAGC TCAGCTTCGA TAGAACCTTC ACCCATCCTT TCCAGAAGAG CAAAAGTCAT 
0361 GCTTCGAGCA GCAAACACAC CGGCATGTCC ATCCTCGATG ATCTGCCCAC TTTgGGCAAC 
0421 GCCAAGGAGA ACTTGGAGAG CGCTGTGCAA ACGCAGAATG TCCGCGATGT TCTGACCAAC 
0481 TGTCGGTATT TGGAGGATGA

in silico PCR Fragment

0001 GTGCCAGTAC ATCCACTTAG CCAGGATCCC ACGGCCGCTT GGAGGGAGAT TAGCAAAACG 
0061 CAGCGCGTGA TCAAGGTGAC AATGAAGCCA CCGACCACCA CGGTGGCGCC CAACAAGGCA 
0121 CGAGTGGTTT GCATGTCCGA CACGCACTCC CTGACGCCCT ACATCAAGTT CGATATACCA 
0181 GATGGCGATA TATTCATACA TGCCGGCGAT TTTACCAAGT GCGGCCAACT GGAGGAGGTG 
0241 GAGGAGTTTA ACACCTGGAT TGGAGCACTG CCCCATCGCC ACAAGATCGT GATTGCTGGC 
0301 AATCATGAGC TCAGCTTCGA TAGAACCTTC ACCCATCCTT TCCAGAAGAG CAAAAGTCAT 
0361 GCTTCGAGCA GCAAACACAC CGGCATGTCC ATCCTCGATG ATCTGCCCAC TTTGGGCAAC 
0421 GCCAAGGAGA ACTTGGAGAG CGCTGTGCAA ACGCAGAATG TCCGCGATGT TCTGACCAAC 
0481 TGTCGGTATT TGGAGGATGA

Assemble Data

<PRE>

16717R-2.IR_full        1   TGCCAGTACATCCACTTAGCCAGGATCCCACGGCCGCTTGGAGGGAGATTAGCAAAACG 59
                            |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16717R-2.in silico      1   TGCCAGTACATCCACTTAGCCAGGATCCCACGGCCGCTTGGAGGGAGATTAGCAAAACG 59


16717R-2.IR_full       61  CAGCG<span class="snp"><tt>N</tt></span>GTG<span class="snp"><tt>N</tt></span>TCAAGGTGACAATGAAGCCACCGACCACCACGGTGGCGCCCAACAAGGCA 119
                           |||||<span class="snp"><tt>&nbsp;</tt></span>|||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||
16717R-2.in silico     61  CAGCG<span class="snp"><tt>C</tt></span>GTG<span class="snp"><tt>A</tt></span>TCAAGGTGACAATGAAGCCACCGACCACCACGGTGGCGCCCAACAAGGCA 119


16717R-2.IR_full       121 CGAGTGGTTTGCATGTCCGACACGCACTCCCTGACGCCCTACATCAAGTTCGATATACCA 179
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16717R-2.in silico     121 CGAGTGGTTTGCATGTCCGACACGCACTCCCTGACGCCCTACATCAAGTTCGATATACCA 179


16717R-2.IR_full       181 GATGGCGATATATTCATACATGCCGGCGATTTTACCAAGTGCGGCCAACTGGAGGAGGTG 239
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16717R-2.in silico     181 GATGGCGATATATTCATACATGCCGGCGATTTTACCAAGTGCGGCCAACTGGAGGAGGTG 239


16717R-2.IR_full       241 GAGGAGTTTAACACCTGGATTGGAGCACTGCCCCATCGCCACAAGATCGTGATTGCTGGC 299
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16717R-2.in silico     241 GAGGAGTTTAACACCTGGATTGGAGCACTGCCCCATCGCCACAAGATCGTGATTGCTGGC 299


16717R-2.IR_full       301 AATCATGAGCTCAGCTTCGATAGAACCTTCACCCATCCTTTCCAGAAGAGCAAAAGTCAT 359
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16717R-2.in silico     301 AATCATGAGCTCAGCTTCGATAGAACCTTCACCCATCCTTTCCAGAAGAGCAAAAGTCAT 359


16717R-2.IR_full       361 GCTTCGAGCAGCAAACACACCGGCATGTCCATCCTCGATGATCTGCCCACTTTGGGCAAC 419
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16717R-2.in silico     361 GCTTCGAGCAGCAAACACACCGGCATGTCCATCCTCGATGATCTGCCCACTTTGGGCAAC 419


16717R-2.IR_full       421 GCCAAGGAGAACTTGGAGAGCGCTGTGCAAACGCAGAATGTCCGCGATGTTCTGACCAAC 479
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16717R-2.in silico     421 GCCAAGGAGAACTTGGAGAGCGCTGTGCAAACGCAGAATGTCCGCGATGTTCTGACCAAC 479


16717R-2.IR_full       481 TGTCGGTATTTGGAGGATGA 499
                           ||||||||||||||||||||
16717R-2.in silico     481 TGTCGGTATTTGGAGGATGA 499

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	2	NM_140086.1	CG16717-RA (CG16717), mRNA
0.2	1	3	2	6	NM_167470.1	CG18102-RD, transcript variant D (shi), mRNA
0.2	1	3	2	6	NM_206742.1	CG18102-RG, transcript variant G (shi), mRNA
0.2	1	3	2	6	NM_206745.2	CG18102-RA, transcript variant A (shi), mRNA
0.2	1	3	2	6	NM_206743.1	CG18102-RF, transcript variant F (shi), mRNA
0.2	1	3	2	6	NM_206744.2	CG18102-RE, transcript variant E (shi), mRNA
0.2	1	3	2	6	NM_001042813.1	CG18102-RH, transcript variant H (shi), mRNA
0.2	1	3	2	6	NM_167471.3	CG18102-RB, transcript variant B (shi), mRNA
0.2	1	3	2	6	NM_001042814.1	CG18102-RI, transcript variant I (shi), mRNA
0.2	1	3	2	6	NM_080114.3	CG18102-RC, transcript variant C (shi), mRNA
0	0	5	5	3	NM_141388.2	CG1249-RA (CG1249), mRNA
0	0	5	3	12	NM_167620.2	CG32547-RA (CG32547), mRNA
0	0	4	2	5	NM_078803.1	CG4105-RA (Cyp4e3), mRNA
0	0	3	2	2	NM_131930.1	CG15572-RA (CG15572), mRNA
0	0	2	7	2	NM_132909.1	CG9782-RA (CG9782), mRNA
0	0	2	4	4	NM_143114.1	CG10514-RA (CG10514), mRNA
0	0	2	4	2	NM_133117.2	CG7406-RA (CG7406), mRNA
0	0	2	3	6	NM_132521.2	CG1578-RA (CG1578), mRNA
0	0	2	3	5	NM_143459.1	CG1964-RA (Kul), mRNA
0	0	2	3	3	NM_170139.1	CG31132-RA (BRWD3), mRNA
0	0	2	2	4	NM_057604.3	CG1825-RA (Map60), mRNA
0	0	1	4	4	NM_135400.1	CG9287-RA (CG9287), mRNA
0	0	1	3	8	NM_135281.1	CG6630-RA (CG6630), mRNA
0	0	1	3	6	NM_142448.2	CG8064-RA (CG8064), mRNA
0	0	1	3	5	NM_176223.2	CG30115-RD, transcript variant D (CG30115), mRNA
0	0	1	3	4	NM_132066.1	CG4320-RA (raptor), mRNA
0	0	1	3	3	NM_079352.2	CG3329-RA (Prosbeta2), mRNA
0	0	1	3	3	NM_079222.2	CG8339-RA (sfl), mRNA
0	0	1	2	3	NM_132317.1	CG12121-RA (CG12121), mRNA
0	0	0	8	5	NM_169939.1	CG5670-RD, transcript variant D (Atpalpha), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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