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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
16711R-3 |
| Symbol |
CG16711 |
Full Name |
CG16711 |
| CG No |
CG16711 |
Old CG No |
CG16711 |
| Synonyms |
CG16711 |
| Accession No (Link to NCBI) |
NM_140090.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGCTGCTGCTAGCCAGGATGAG |
| Primer Seq. 3' |
aatctagaggtaccGCTGTGGATGTGACGTGCGTT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GCTGCTGCTA GCCAGGATGA GCCGGAGGNG AAGTGCGAAT AACAACAATA ATCCCACAGC
0061 AACAACAACA ACAATTAACG GAGGGCATCA TCAAGCAGGA AACTGGAGGC CGCTCCCGCC
0121 GGTTTGCCAC CGAAAATCGG AGCGCCGGAG AGTGGCCCGT GATATATTCC TGGTCTTCGA
0181 GGAGCAGACC ACCCGGCGAC GAGGGAAGGG AGCACGGGGC AAGGCCACAA GGGCAAAGCA
0241 AAAGTTCGGA CACCTGAAGG TGGCCAATAC GCCGCGCATC AATAAGAATG TGCTGCGCGA
0301 CATTAAAACG GAACCAAAGT GCAGCTCGGA AGCCGTTGTG CCGGCTGCTG CCCCAGCTAT
0361 CACACCCATT CCCGCACTGA ATCCCCCAAT TCCTCCGCAA ATAGCCGCGC TCAATCCTCC
0421 GTTGCCGGAG GAGCCGACGT TAGCCAAATC CTTGCCGCGA ACgGCGGCCG TAAAATTGGA
0481 AACCAAAACG CACGTCACAT CCACAGC |
| in silico PCR Fragment |
0001 GCTGCTGCTA GCCAGGATGA GCCGGAGGCG -AGTGCGAAT AACAACAATA AT--CACAGC
0061 AACAACAACA ACAA-TAAC- GAGGGCATCA TCAAGCAGG- AACTGGAGGC CGCT-CCGCC
0121 GGTTTGCCAC CGAAAATCGG AGCGCCGGAG AGTGGCCCGT GATATATTCC TGGTCTTCGA
0181 GGAGCAGACC ACCCGGCGAC GAGGGAAGGG AGCACGGGGC AAGGCCACAA GGGCAAAGCA
0241 AAAGTTCGGA CACCTGAAGG TGGCCAATAC GCCGCGCATC AATAAGAATG TGCTGCGCGA
0301 CATTAAAACG GAACCAAAGT GCAGCTCGGA AGCCGTTGTG CCGGCTGCTG CCCCAGCTAT
0361 CACACCCATT CCCGCACTGA ATCCCCCAAT TCCTCCGCAA ATAGCCGCGC TCAATCCTCC
0421 GTTGCCGGAG GAGCCGACGT TAGCCAAATC CTTGCCGCGA ACGGCGGCCG TAAAATTGGA
0481 AACCAAAACG CACGTCACAT CCACAGC |
| Assemble Data |
<PRE>
16711R-3.IR_full 1 GCTGCTGCTAGCCAGGATGAGCCGGAGG<span class="snp"><tt>N</tt></span>G<span class="snp"><tt>A</tt></span>AGTGCGAATAACAACAATAAT<span class="snp"><tt>C</tt></span><span class="snp"><tt>C</tt></span>CACAGC 60
||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|<span class="snp"><tt> </tt></span>|||||||||||||||||||||<span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span>||||||
16711R-3.in silico 1 GCTGCTGCTAGCCAGGATGAGCCGGAGG<span class="snp"><tt>C</tt></span>G<span class="snp"><tt>-</tt></span>AGTGCGAATAACAACAATAAT<span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span>CACAGC 60
16711R-3.IR_full 61 AACAACAACAACAA<span class="snp"><tt>T</tt></span>TAAC<span class="snp"><tt>G</tt></span>GAGGGCATCATCAAGCAGG<span class="snp"><tt>A</tt></span>AACTGGAGGCCGCT<span class="snp"><tt>C</tt></span>CCGCC 120
||||||||||||||<span class="snp"><tt> </tt></span>||||<span class="snp"><tt> </tt></span>|||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||<span class="snp"><tt> </tt></span>|||||
16711R-3.in silico 61 AACAACAACAACAA<span class="snp"><tt>-</tt></span>TAAC<span class="snp"><tt>-</tt></span>GAGGGCATCATCAAGCAGG<span class="snp"><tt>-</tt></span>AACTGGAGGCCGCT<span class="snp"><tt>-</tt></span>CCGCC 120
16711R-3.IR_full 121 GGTTTGCCACCGAAAATCGGAGCGCCGGAGAGTGGCCCGTGATATATTCCTGGTCTTCGA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16711R-3.in silico 121 GGTTTGCCACCGAAAATCGGAGCGCCGGAGAGTGGCCCGTGATATATTCCTGGTCTTCGA 180
16711R-3.IR_full 181 GGAGCAGACCACCCGGCGACGAGGGAAGGGAGCACGGGGCAAGGCCACAAGGGCAAAGCA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16711R-3.in silico 181 GGAGCAGACCACCCGGCGACGAGGGAAGGGAGCACGGGGCAAGGCCACAAGGGCAAAGCA 240
16711R-3.IR_full 241 AAAGTTCGGACACCTGAAGGTGGCCAATACGCCGCGCATCAATAAGAATGTGCTGCGCGA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16711R-3.in silico 241 AAAGTTCGGACACCTGAAGGTGGCCAATACGCCGCGCATCAATAAGAATGTGCTGCGCGA 300
16711R-3.IR_full 301 CATTAAAACGGAACCAAAGTGCAGCTCGGAAGCCGTTGTGCCGGCTGCTGCCCCAGCTAT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16711R-3.in silico 301 CATTAAAACGGAACCAAAGTGCAGCTCGGAAGCCGTTGTGCCGGCTGCTGCCCCAGCTAT 360
16711R-3.IR_full 361 CACACCCATTCCCGCACTGAATCCCCCAATTCCTCCGCAAATAGCCGCGCTCAATCCTCC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16711R-3.in silico 361 CACACCCATTCCCGCACTGAATCCCCCAATTCCTCCGCAAATAGCCGCGCTCAATCCTCC 420
16711R-3.IR_full 421 GTTGCCGGAGGAGCCGACGTTAGCCAAATCCTTGCCGCGAACGGCGGCCGTAAAATTGGA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16711R-3.in silico 421 GTTGCCGGAGGAGCCGACGTTAGCCAAATCCTTGCCGCGAACGGCGGCCGTAAAATTGGA 480
16711R-3.IR_full 481 AACCAAAACGCACGTCACATCCACAGC 507
|||||||||||||||||||||||||||
16711R-3.in silico 481 AACCAAAACGCACGTCACATCCACAGC 507
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
3 |
38 |
NM_168361.1 |
CG16711-RB, transcript variant B (CG16711), mRNA |
| 100 |
482 |
0 |
3 |
38 |
NM_140090.1 |
CG16711-RA, transcript variant A (CG16711), mRNA |
| 0.82 |
4 |
2 |
11 |
27 |
NM_079861.2 |
CG1455-RA (CanA1), mRNA |
| 0.62 |
3 |
14 |
70 |
171 |
NM_132370.1 |
CG2989-RA (CG2989), mRNA |
| 0.62 |
3 |
13 |
18 |
33 |
NM_143443.2 |
CG11470-RA (CG11470), mRNA |
| 0.62 |
3 |
5 |
12 |
23 |
NM_166441.2 |
CG30387-RB, transcript variant B (CG30387), mRNA |
| 0.62 |
3 |
5 |
12 |
22 |
NM_137730.2 |
CG30387-RA, transcript variant A (CG30387), mRNA |
| 0.62 |
3 |
5 |
12 |
22 |
NM_166442.2 |
CG30387-RC, transcript variant C (CG30387), mRNA |
| 0.62 |
3 |
5 |
5 |
21 |
NM_169778.1 |
CG31246-RA (CG31246), mRNA |
| 0.62 |
3 |
3 |
13 |
27 |
NM_078663.2 |
CG5529-RA (B-H1), mRNA |
| 0.62 |
3 |
3 |
2 |
15 |
NM_167373.1 |
CG32629-RA (CG32629), mRNA |
| 0.41 |
2 |
8 |
28 |
42 |
NM_142444.1 |
CG18599-RA (CG18599), mRNA |
| 0.41 |
2 |
5 |
5 |
12 |
NM_132655.1 |
CG1662-RA (CG1662), mRNA |
| 0.41 |
2 |
4 |
3 |
24 |
NM_143154.3 |
CG5053-RA (CG5053), mRNA |
| 0.41 |
2 |
3 |
10 |
5 |
NM_166991.2 |
CG32779-RA (CG32779), mRNA |
| 0.41 |
2 |
3 |
4 |
26 |
NM_001032244.1 |
CG32904-RA, transcript variant A (seq), mRNA |
| 0.41 |
2 |
2 |
6 |
28 |
NM_078660.2 |
CG5424-RB, transcript variant B (f), mRNA |
| 0.41 |
2 |
2 |
6 |
28 |
NM_001042819.1 |
CG5424-RF, transcript variant F (f), mRNA |
| 0.41 |
2 |
2 |
6 |
28 |
NM_176745.1 |
CG5424-RD, transcript variant D (f), mRNA |
| 0.41 |
2 |
2 |
6 |
26 |
NM_176746.1 |
CG5424-RC, transcript variant C (f), mRNA |
| 0.2 |
1 |
16 |
52 |
103 |
NM_079002.2 |
CG3905-RA (Su(z)2), mRNA |
| 0.2 |
1 |
15 |
56 |
150 |
NM_078592.2 |
CG11172-RA (NFAT), mRNA |
| 0.2 |
1 |
12 |
50 |
139 |
NM_169786.1 |
CG7847-RB, transcript variant B (sr), mRNA |
| 0.2 |
1 |
12 |
49 |
137 |
NM_079671.2 |
CG7847-RA, transcript variant A (sr), mRNA |
| 0.2 |
1 |
12 |
33 |
48 |
NM_132161.4 |
CG1659-RA (unc-119), mRNA |
| 0.2 |
1 |
11 |
27 |
48 |
NM_168964.2 |
CG11352-RB, transcript variant B (jim), mRNA |
| 0.2 |
1 |
11 |
27 |
48 |
NM_143699.2 |
CG11352-RC, transcript variant C (jim), mRNA |
| 0.2 |
1 |
10 |
37 |
70 |
NM_137212.2 |
CG30089-RA (CG30089), mRNA |
| 0.2 |
1 |
9 |
51 |
179 |
NM_167335.1 |
CG4013-RC, transcript variant C (Smr), mRNA |
| 0.2 |
1 |
9 |
51 |
179 |
NM_167334.1 |
CG4013-RB, transcript variant B (Smr), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| |
|
| DB |
 |
| +- |
|
| DC |
 |
| +- |
|
| DM |
 |
| +- |
|
| VM |
 |
| |
|
| VB |
 |
| |
|
|
Caspase-3 signal
| N |
|
| ++ |
|
| DB |
|
| ++ |
|
| DC |
|
| ++ |
|
| DM |
|
| + |
|
| VM |
|
| |
|
| VB |
|
| |
|
|
|
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
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|
