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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 1657R-4 
 Symbol CG1657  Full Name CG1657 
 CG No CG1657  Old CG No CG1657 
 Synonyms CG1657 
 Accession No (Link to NCBI)  
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees early pupal lethal 
 Map Viewer
 Reference
[Please submit your publication]
 
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgAGATCATGGACCTGGCCCGCA 
 Primer Seq. 3'
aatctagaggtaccGGGCTGTTCGCTGGTCAGCAA 
 Predicted Fragment Size
500 
 IR fragment full Seq
 
 in silico PCR Fragment
 
 Assemble Data

1657R-4.IR full       1   AGATCATGGACCTGGCCCGCACTCTCCGCCNNNAGCAGCTCTTCATCCAGCAGGAGCAGG 60
                          ||||||||||||||||||||||||||||||   |||||||||||||||||||||||||||
1657R-4.in silico     1   AGATCATGGACCTGGCCCGCACTCTCCGCCAGGAGCAGCTCTTCATCCAGCAGGAGCAGG 60


1657R-4.IR full       61  CGGCCTTTGCCCAGCTCACTGGAGCCTTTGAAACCAATGCAGGGACCATAACCAAGTTGG 120
                          ||||||||||||||||||||||||||||||| | ||||||||||||||||||||||||||
1657R-4.in silico     61  CGGCCTTTGCCCAGCTCACTGGAGCCTTTGAGAGCAATGCAGGGACCATAACCAAGTTGG 120


1657R-4.IR full       121 CCTTTGTGTGTGCCCAGCAGCGGCAGATACTCAATGAGCTGCTCCTGGCGCGCACAGATC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1657R-4.in silico     121 CCTTTGTGTGTGCCCAGCAGCGGCAGATACTCAATGAGCTGCTCCTGGCGCGCACAGATC 180


1657R-4.IR full       181 AGGATCCGCTGCTCTTCTGCCGCCGAGCGAGTGCCTACGATAGTGCCCAATTCGTGGACG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1657R-4.in silico     181 AGGATCCGCTGCTCTTCTGCCGCCGAGCGAGTGCCTACGATAGTGCCCAATTCGTGGACG 240


1657R-4.IR full       241 CCAAGCAGTTGCTGCCCTATGAGCACGCCTTGGCCTACGAAGATCTGTTCAACTACTTGT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1657R-4.in silico     241 CCAAGCAGTTGCTGCCCTATGAGCACGCCTTGGCCTACGAAGATCTGTTCAACTACTTGT 300


1657R-4.IR full       301 ATAATACACCCTATTTGTTGGCCCTATCCCTGGCCACCGCGGATCGCTTATCCCTGCTCT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1657R-4.in silico     301 ATAATACACCCTATTTGTTGGCCCTATCCCTGGCCACCGCGGATCGCTTATCCCTGCTCT 360


1657R-4.IR full       361 CGGCCAGCCAGCTTGGCCAAATTATCAATACCATAGCGACGGGACTGTATG-CAATGCCA 420
                          ||||||||||||||||||||||||| ||||||||||||||||||||||||| ||||||||
1657R-4.in silico     361 CGGCCAGCCAGCTTGGCCAAATTATTAATACCATAGCGACGGGACTGTATGGCAATGCCA 420


1657R-4.IR full       421 TCAATACCAAGGATGTGGAACTGCTGCTTAAATTGCTGCGGGAACTGATTGAAATCCAAT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1657R-4.in silico     421 TCAATACCAAGGATGTGGAACTGCTGCTTAAATTGCTGCGGGAACTGATTGAAATCCAAT 480


1657R-4.IR full       481 TGCTGACCAGCGAACAGCC- 500
                          ||||||||||||||||||| 
1657R-4.in silico     481 TGCTGACCAGCGAACAGCCC 500

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
 

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