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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 1616R-1 
 Symbol dpa  Full Name disc proliferation abnormal 
 CG No CG1616  Old CG No CG1616 
 Synonyms Mcm4, MCM4, DmMcm4, 43Ca, DmMCM4, Dpa, l(2)43Ca, CG1616, dpa 
 Accession No (Link to NCBI) NM_057837.3 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees larval lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Kohzaki H.<br> The function of replication and SCF complex during Drosophila wing development.<br> Front Biosci (Landmark Ed) (2018) 23(12) 2235-2244 [ PubMed ID = 29772558 ] [ RRC reference ]

Bauke AC, Sasse S, Matzat T, Klämbt C.<br> A transcriptional network controlling glial development in the Drosophila visual system.<br> Development (2015) 142(12) 2184-93 [ PubMed ID = 26015542 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgAGGATTGGGTGCGAACATGAG 
 Primer Seq. 3' 
aatctagaggtaccCCTGCGGATAGCAGATCAACT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 AGGATTGGGT GCGAACATGA GCGAGATCGA TCTGAGTTCG CCGCTGAACT ACGGCACCCC 
0061 CAGCTCGATG GGGTCCATTC GAACCCCTCG TTCCGGAATT AGAGGCACTC CGCTGCGGGC 
0121 GCGGCCCGAT AATAAGAACG GACAAGCGCA TCCGCCAGGT GGCCATCGGT GGGGGCTCTG 
0181 GGCTCGAACC AATTCCTGAG AAGGGCTCTG AAACCACCGA TCCTGTATCG GAATCTTCGC 
0241 AAGCACCTCA GCTCGTCGTC TGGGGAACCA ATGTTGTTGT CAGCCAGTGC AAATCCAAGT 
0301 TCAAGTCGTT TATTATGCGC TTTATTGACC CCAGTGCCGA GCAGGACGAA ATCTCCGAGA 
0361 ATATTGATGT TAACCAGCCA CTGTATCTTC AGAAGCTGGA GGAGATTCAC ACATTGGAGG 
0421 AGCCCTATCT GAATCTCAAC TGTGCCCATC TCAAGACCTT TGATCAGGAC TTGTACCGCC 
0481 AGTTGATCTG CTATCCGCAG G 
 in silico PCR Fragment 
0001 AGGATTGGGT GCGAACATGA GCGAGATCGA TCTGAGTTCG CCGCTGAACT ACGGCACCCC 
0061 CAGCTCGATG GGGTCCATTC GAACCCCTCG TTCCGGAATT AGAGGCACTC CGCTGCGGGC 
0121 GCGGCCCGAT -ATAAGAACG GACAAGCGCA TCCGCCAGGT GGCCATCGGT GGGGGCTCTG 
0181 GGCTCGAACC AATTCCTGAG AAGGGCTCTG AAACCACCGA TCCTGTATCG GAATCTTCGC 
0241 AAGCACCTCA GCTCGTCGTC TGGGGAACCA ATGTTGTTGT CAGCCAGTGC AAATCCAAGT 
0301 TCAAGTCGTT TATTATGCGC TTTATTGACC CCAGTGCCGA GCAGGACGAA ATCTCCGAGA 
0361 ATATTGATGT TAACCAGCCA CTGTATCTTC AGAAGCTGGA GGAGATTCAC ACATTGGAGG 
0421 AGCCCTATCT GAATCTCAAC TGTGCCCATC TCAAGACCTT TGATCAGGAC TTGTACCGCC 
0481 AGTTGATCTG CTATCCGCAG G 
 Assemble Data 
<PRE>

1616R-1.IR_full       1   AGGATTGGGTGCGAACATGAGCGAGATCGATCTGAGTTCGCCGCTGAACTACGGCACCCC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1616R-1.in silico     1   AGGATTGGGTGCGAACATGAGCGAGATCGATCTGAGTTCGCCGCTGAACTACGGCACCCC 60


1616R-1.IR_full       61  CAGCTCGATGGGGTCCATTCGAACCCCTCGTTCCGGAATTAGAGGCACTCCGCTGCGGGC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1616R-1.in silico     61  CAGCTCGATGGGGTCCATTCGAACCCCTCGTTCCGGAATTAGAGGCACTCCGCTGCGGGC 120


1616R-1.IR_full       121 GCGGCCCGAT<span class="snp"><tt>A</tt></span>ATAAGAACGGACAAGCGCATCCGCCAGGTGGCCATCGGTGGGGGCTCTG 180
                          ||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||
1616R-1.in silico     121 GCGGCCCGAT<span class="snp"><tt>-</tt></span>ATAAGAACGGACAAGCGCATCCGCCAGGTGGCCATCGGTGGGGGCTCTG 180


1616R-1.IR_full       181 GGCTCGAACCAATTCCTGAGAAGGGCTCTGAAACCACCGATCCTGTATCGGAATCTTCGC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1616R-1.in silico     181 GGCTCGAACCAATTCCTGAGAAGGGCTCTGAAACCACCGATCCTGTATCGGAATCTTCGC 240


1616R-1.IR_full       241 AAGCACCTCAGCTCGTCGTCTGGGGAACCAATGTTGTTGTCAGCCAGTGCAAATCCAAGT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1616R-1.in silico     241 AAGCACCTCAGCTCGTCGTCTGGGGAACCAATGTTGTTGTCAGCCAGTGCAAATCCAAGT 300


1616R-1.IR_full       301 TCAAGTCGTTTATTATGCGCTTTATTGACCCCAGTGCCGAGCAGGACGAAATCTCCGAGA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1616R-1.in silico     301 TCAAGTCGTTTATTATGCGCTTTATTGACCCCAGTGCCGAGCAGGACGAAATCTCCGAGA 360


1616R-1.IR_full       361 ATATTGATGTTAACCAGCCACTGTATCTTCAGAAGCTGGAGGAGATTCACACATTGGAGG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1616R-1.in silico     361 ATATTGATGTTAACCAGCCACTGTATCTTCAGAAGCTGGAGGAGATTCACACATTGGAGG 420


1616R-1.IR_full       421 AGCCCTATCTGAATCTCAACTGTGCCCATCTCAAGACCTTTGATCAGGACTTGTACCGCC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1616R-1.in silico     421 AGCCCTATCTGAATCTCAACTGTGCCCATCTCAAGACCTTTGATCAGGACTTGTACCGCC 480


1616R-1.IR_full       481 AGTTGATCTGCTATCCGCAGG 501
                          |||||||||||||||||||||
1616R-1.in silico     481 AGTTGATCTGCTATCCGCAGG 501

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_057837.3  CG1616-RA (dpa), mRNA 
0   0  3  3  3  NM_166141.2  CG8421-RD, transcript variant D (Asph), mRNA 
0   0  3  3  3  NM_176185.1  CG8421-RE, transcript variant E (Asph), mRNA 
0   0  2  2  5  NM_078517.2  CG2175-RA, transcript variant A (dec-1), mRNA 
0   0  2  2  5  NM_167133.1  CG2175-RC, transcript variant C (dec-1), mRNA 
0   0  2  2  5  NM_167132.1  CG2175-RB, transcript variant B (dec-1), mRNA 
0   0  1  4  9  NM_078645.2  CG4252-RA (mei-41), mRNA 
0   0  1  4  2  NM_143048.1  CG11089-RA (CG11089), mRNA 
0   0  1  3  5  NM_142992.1  CG6454-RB, transcript variant B (CG6454), mRNA 
0   0  1  3  5  NM_170141.1  CG6454-RA, transcript variant A (CG6454), mRNA 
0   0  1  3  3  NM_145105.2  CG17611-RA (eIF6), mRNA 
0   0  1  3  1  NM_143459.1  CG1964-RA (Kul), mRNA 
0   0  1  2  3  NM_140420.1  CG9040-RA (CG9040), mRNA 
0   0  1  2  2  NM_135830.3  CG16863-RA, transcript variant A (CG16863), mRNA 
0   0  1  2  2  NM_206430.1  CG2666-RC, transcript variant C (kkv), mRNA 
0   0  1  2  2  NM_079509.1  CG2666-RA, transcript variant A (kkv), mRNA 
0   0  1  2  2  NM_169052.1  CG2666-RB, transcript variant B (kkv), mRNA 
0   0  1  2  2  NM_001042896.1  CG16863-RB, transcript variant B (CG16863), mRNA 
0   0  0  6  3  NM_137192.1  CG12958-RA (CG12958), mRNA 
0   0  0  5  5  NM_167241.1  CG1799-RC, transcript variant C (ras), mRNA 
0   0  0  5  5  NM_167240.1  CG1799-RA, transcript variant A (ras), mRNA 
0   0  0  5  5  NM_079907.4  CG1799-RB, transcript variant B (ras), mRNA 
0   0  0  5  2  NM_141379.1  CG1157-RA (Osi15), mRNA 
0   0  0  4  8  NM_137690.2  CG10543-RA, transcript variant A (CG10543), mRNA 
0   0  0  4  6  NM_058149.3  CG3352-RA (ft), mRNA 
0   0  0  4  5  NM_164387.1  CG3022-RB, transcript variant B (GABA-B-R3), mRNA 
0   0  0  4  5  NM_078723.2  CG3022-RA, transcript variant A (GABA-B-R3), mRNA 
0   0  0  4  4  NM_166696.1  CG9071-RB, transcript variant B (NaCP60E), mRNA 
0   0  0  4  4  NM_079134.5  CG9071-RA, transcript variant A (NaCP60E), mRNA 
0   0  0  4  3  NM_170062.1  CG10868-RB, transcript variant B (orb), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway dme03030 : DNA replication

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