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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 1605R-2 
 Symbol az2  Full Name az2 
 CG No CG1605  Old CG No CG1605 
 Synonyms zf43C, CG1605, az2 
 Accession No (Link to NCBI) NM_136445.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		 
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGGTGGTTTGCGGGAACATTTT 
 Primer Seq. 3' 
aatctagaggtaccAGAGGCATGTCTGCTGCTTAT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GGTGGTTTGC GGGAACATTT TGGCCAGGAA CAACTTCGAG CACTTTGTTC TGGCGTGTGC 
0061 TCACCAAAAT GACTGCTACG TGGAGATGGA GTTCAAGCGG TGGCGANGAG TTCATCGTGC 
0121 ACGTCAAGGA GCACCTGGAA GCAAATCCAA GCATGCAGCA AGAGCTCACT ACTTCCGAAG 
0181 TCATTTCGTC AGCCAAATGC GAGGAGGAGG TCTCGCCAGC CAAGGTGTTC ATAGTGGAGG 
0241 AGCCTCCGGA AAATTGCCTG GCTGAAGACA TGTTCGTTGA CGTGCCACCT TCATCAGAGA 
0301 ACGACGAGAG CACCAGCCCG GTGGAGGAGG AGGAGGAAGA AGACGACGAG GTGGACTCCT 
0361 CCTCGATGAC AGTTGACTGC GAATTGGGCA CCAGAAACTC CACACACTTT GCTCCTTTAA 
0421 CCAAATTCAA TCCTACGTTT TACCGACGCA GTCCTCGGAT CACACAGTTC ATTGAACTGT 
0481 ATAAGCAGCA GACATGCCTC T 
 in silico PCR Fragment 
0001 GGTGGTTTGC GGGAACATTT TGGCCAGGAA CAACTTCGAG CACTTTGTTC TGGCGTGTGC 
0061 TCACCAAAAT GACTGCTACG TGGAGATGGA GTTCAAGCGG TGGCGA-GAG TTCATCGTGC 
0121 ACGTCAAGGA GCACCTGGAA GCAAATCCAA GCATGCAGCA AGAGCTCACT ACTTCCGAAG 
0181 TCATTTCGTC AGCCAAATGC GAGGAGGAGG TCTCGCCAGC CAAGGTGTTC ATAGTGGAGG 
0241 AGCCTCCGGA AAATTGCCTG GCTGAAGACA TGTTCGTTGA CGTGCCACCT TCATCAGAGA 
0301 ACGACGAGAG CACCAGCCCG GTGGAGGAGG AGGAGGAAGA AGACGACGAG GTGGACTCCT 
0361 CCTCGATGAC AGTTGACTGC GAATTGGGCA CCAGAAACTC CACACACTTT GCTCCTTTAA 
0421 CCAAATTCAA TCCTACGTTT TACCGACGCA GTCCTCGGAT CACACAGTTC ATTGAACTGT 
0481 ATAAGCAGCA GACATGCCTC T 
 Assemble Data 
<PRE>

1605R-2.IR_full       1   GGTGGTTTGCGGGAACATTTTGGCCAGGAACAACTTCGAGCACTTTGTTCTGGCGTGTGC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1605R-2.in silico     1   GGTGGTTTGCGGGAACATTTTGGCCAGGAACAACTTCGAGCACTTTGTTCTGGCGTGTGC 60


1605R-2.IR_full       61  TCACCAAAATGACTGCTACGTGGAGATGGAGTTCAAGCGGTGGCGA<span class="snp"><tt>N</tt></span>GAGTTCATCGTGC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||
1605R-2.in silico     61  TCACCAAAATGACTGCTACGTGGAGATGGAGTTCAAGCGGTGGCGA<span class="snp"><tt>-</tt></span>GAGTTCATCGTGC 120


1605R-2.IR_full       121 ACGTCAAGGAGCACCTGGAAGCAAATCCAAGCATGCAGCAAGAGCTCACTACTTCCGAAG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1605R-2.in silico     121 ACGTCAAGGAGCACCTGGAAGCAAATCCAAGCATGCAGCAAGAGCTCACTACTTCCGAAG 180


1605R-2.IR_full       181 TCATTTCGTCAGCCAAATGCGAGGAGGAGGTCTCGCCAGCCAAGGTGTTCATAGTGGAGG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1605R-2.in silico     181 TCATTTCGTCAGCCAAATGCGAGGAGGAGGTCTCGCCAGCCAAGGTGTTCATAGTGGAGG 240


1605R-2.IR_full       241 AGCCTCCGGAAAATTGCCTGGCTGAAGACATGTTCGTTGACGTGCCACCTTCATCAGAGA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1605R-2.in silico     241 AGCCTCCGGAAAATTGCCTGGCTGAAGACATGTTCGTTGACGTGCCACCTTCATCAGAGA 300


1605R-2.IR_full       301 ACGACGAGAGCACCAGCCCGGTGGAGGAGGAGGAGGAAGAAGACGACGAGGTGGACTCCT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1605R-2.in silico     301 ACGACGAGAGCACCAGCCCGGTGGAGGAGGAGGAGGAAGAAGACGACGAGGTGGACTCCT 360


1605R-2.IR_full       361 CCTCGATGACAGTTGACTGCGAATTGGGCACCAGAAACTCCACACACTTTGCTCCTTTAA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1605R-2.in silico     361 CCTCGATGACAGTTGACTGCGAATTGGGCACCAGAAACTCCACACACTTTGCTCCTTTAA 420


1605R-2.IR_full       421 CCAAATTCAATCCTACGTTTTACCGACGCAGTCCTCGGATCACACAGTTCATTGAACTGT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1605R-2.in silico     421 CCAAATTCAATCCTACGTTTTACCGACGCAGTCCTCGGATCACACAGTTCATTGAACTGT 480


1605R-2.IR_full       481 ATAAGCAGCAGACATGCCTCT 501
                          |||||||||||||||||||||
1605R-2.in silico     481 ATAAGCAGCAGACATGCCTCT 501

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  26  19  NM_136445.2  CG1605-RA (az2), mRNA 
0.82   4  3  15  57  NM_132370.1  CG2989-RA (CG2989), mRNA 
0.62   3  13  33  69  NM_138025.2  CG3121-RA (CG3121), mRNA 
0.41   2  16  53  143  NM_001014737.1  CG7107-RG, transcript variant G (up), mRNA 
0.41   2  16  53  143  NM_080349.2  CG7107-RA, transcript variant A (up), mRNA 
0.41   2  16  53  143  NM_167375.1  CG7107-RB, transcript variant B (up), mRNA 
0.41   2  16  53  140  NM_167376.1  CG7107-RD, transcript variant D (up), mRNA 
0.41   2  16  53  138  NM_001014738.1  CG7107-RF, transcript variant F (up), mRNA 
0.41   2  16  53  138  NM_001014739.1  CG7107-RE, transcript variant E (up), mRNA 
0.41   2  13  6  23  NM_166289.1  CG5186-RB, transcript variant B (slim), mRNA 
0.41   2  13  6  23  NM_079058.2  CG5186-RA, transcript variant A (slim), mRNA 
0.41   2  4  4  5  NM_079276.2  CG4466-RA (Hsp27), mRNA 
0.2   1  4  8  20  NM_139645.2  CG15021-RA (CG15021), mRNA 
0.2   1  2  6  15  NM_137203.2  CG8192-RA (CG8192), mRNA 
0.2   1  2  2  8  NM_168955.1  CG6838-RB, transcript variant B (CG6838), mRNA 
0.2   1  2  2  8  NM_141152.1  CG6838-RA, transcript variant A (CG6838), mRNA 
0   0  11  30  65  NM_132012.1  CG15776-RA (CG15776), mRNA 
0   0  10  14  14  NM_138041.2  CG4049-RA (CG4049), mRNA 
0   0  9  15  48  NM_143060.1  CG13647-RA (CG13647), mRNA 
0   0  7  20  36  NM_078816.2  CG7279-RA (Lip1), mRNA 
0   0  7  15  57  NM_132346.1  CG3003-RB (CG3003), mRNA 
0   0  7  14  31  NM_080335.2  CG2984-RA (Pp2C1), mRNA 
0   0  7  9  29  NM_132908.2  CG4453-RA (Nup153), mRNA 
0   0  7  8  30  NM_131978.1  CG15465-RA (CG15465), mRNA 
0   0  7  7  23  NM_001007096.1  CG8742-RB, transcript variant B (Gyc76C), mRNA 
0   0  7  7  23  NM_001007095.1  CG8742-RC, transcript variant C (Gyc76C), mRNA 
0   0  7  7  23  NM_079441.3  CG8742-RA, transcript variant A (Gyc76C), mRNA 
0   0  7  5  9  NM_167643.1  CG32537-RA (CG32537), mRNA 
0   0  6  23  55  NM_170139.1  CG31132-RA (BRWD3), mRNA 
0   0  6  9  20  NM_167218.1  CG32685-RC (CG32685), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway

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