NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

1602R-2

Symbol

CG1602

Full Name

CG1602

CG No

CG1602

Old CG No

CG1602

Synonyms

CG1602

Accession No (Link to NCBI)

NM_136447.2

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCTTGCGGCCTTATTTGTGTGA

Primer Seq. 3'

aatctagaggtaccATTCCAAAGGCACGGGTGTTC

Predicted Fragment Size

IR fragment full Seq

0001 CTTGCGGCCT TATTTGTGTG AGCAGGGACT ACGAGAAGTT CCTGATGCGC TGCTCCTACT 
0061 GTCCAACGGA CGTGGAGGTG GCCCAGTGGC AGGAGTTTGT GCTGCACATC CGCAACGTGC 
0121 ATAGCAAAGT AAGGAGCATG GAAGACGACT TCGAGCCAGT GGGGGAAAAT TCTACGGAGT 
0181 CTGGGCACGT AGATCCTCCA GAGGAGAAAC CCCAAGCGGA AGAGTCGATA TTCCAGCAAG 
0241 ACGAATCGGC GGACGGAGCC TGTGTACAGC TGCATATTGA CGCAGTGCGT TCGTCGACAA 
0301 ACGAGACTGA GGAGTCGGAC GTATCTTTGG TTACAGATGA AGAAGAATCC GTCCAGGACG 
0361 AGGATCCAAG TGAACCAGCT CTTGATGATG CCAATTTAAA AACCACACCC ACATACAAGT 
0421 TTCATCCATC CTTCTTTCGC CGAGATCACC GAACCATAAA GTTCATAGAG ATCTACAAGG 
0481 AACACCCGTG CCTTTGGGAA TGGT

in silico PCR Fragment

0001 CTTGCGGCCT TATTTGTGTG AGCAGGGACT ACGAGAAGTT CCTGATGCGC TGCTCCTACT 
0061 GTCCAACGGA CGTGGAGGTG GCCCAGTGGC AGGAGTTTGT GCTGCACATC CGCAACGTGC 
0121 ATAGCAAAGT AAGGAGCATG GAAGACGACT TCGAGCCAGT GGGGGAAAAT TCTACGGAGT 
0181 CTGGGCACGT AGATCCTCCA GAGGAGAAAC CCCAAGCGGA AGAGTCGATA TTCCAGCAAG 
0241 ACGAATCGGC GGACGGAGCC TGTGTACAGC TGCATATTGA CGCAGTGCGT TCGTCGACAA 
0301 ACGAGACTGA GGAGTCGGAC GTATCTTTGG TTACAGATGA AGAAGAATCC GTCCAGGACG 
0361 AGGATCCAAG TGAACCAGCT CTTGATGATG CCAATTTAAA AACCACACCC ACATACAAGT 
0421 TTCATCCATC CTTCTTTCGC CGAGATCACC GAACCATAAA GTTCATAGAG ATCTACAAGG 
0481 AACACCCGTG CCTTTGGAA- ---T

Assemble Data

<PRE>

1602R-2.IR_full       1   CTTGCGGCCTTATTTGTGTGAGCAGGGACTACGAGAAGTTCCTGATGCGCTGCTCCTACT 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1602R-2.in silico     1   CTTGCGGCCTTATTTGTGTGAGCAGGGACTACGAGAAGTTCCTGATGCGCTGCTCCTACT 60


1602R-2.IR_full       61  GTCCAACGGACGTGGAGGTGGCCCAGTGGCAGGAGTTTGTGCTGCACATCCGCAACGTGC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1602R-2.in silico     61  GTCCAACGGACGTGGAGGTGGCCCAGTGGCAGGAGTTTGTGCTGCACATCCGCAACGTGC 120


1602R-2.IR_full       121 ATAGCAAAGTAAGGAGCATGGAAGACGACTTCGAGCCAGTGGGGGAAAATTCTACGGAGT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1602R-2.in silico     121 ATAGCAAAGTAAGGAGCATGGAAGACGACTTCGAGCCAGTGGGGGAAAATTCTACGGAGT 180


1602R-2.IR_full       181 CTGGGCACGTAGATCCTCCAGAGGAGAAACCCCAAGCGGAAGAGTCGATATTCCAGCAAG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1602R-2.in silico     181 CTGGGCACGTAGATCCTCCAGAGGAGAAACCCCAAGCGGAAGAGTCGATATTCCAGCAAG 240


1602R-2.IR_full       241 ACGAATCGGCGGACGGAGCCTGTGTACAGCTGCATATTGACGCAGTGCGTTCGTCGACAA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1602R-2.in silico     241 ACGAATCGGCGGACGGAGCCTGTGTACAGCTGCATATTGACGCAGTGCGTTCGTCGACAA 300


1602R-2.IR_full       301 ACGAGACTGAGGAGTCGGACGTATCTTTGGTTACAGATGAAGAAGAATCCGTCCAGGACG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1602R-2.in silico     301 ACGAGACTGAGGAGTCGGACGTATCTTTGGTTACAGATGAAGAAGAATCCGTCCAGGACG 360


1602R-2.IR_full       361 AGGATCCAAGTGAACCAGCTCTTGATGATGCCAATTTAAAAACCACACCCACATACAAGT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1602R-2.in silico     361 AGGATCCAAGTGAACCAGCTCTTGATGATGCCAATTTAAAAACCACACCCACATACAAGT 420


1602R-2.IR_full       421 TTCATCCATCCTTCTTTCGCCGAGATCACCGAACCATAAAGTTCATAGAGATCTACAAGG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1602R-2.in silico     421 TTCATCCATCCTTCTTTCGCCGAGATCACCGAACCATAAAGTTCATAGAGATCTACAAGG 480


1602R-2.IR_full       481 AACACCCGTGCCTTTGG<span class="snp"><tt>G</tt></span>A<span class="snp"><tt>A</tt></span><span class="snp"><tt>T</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>G</tt></span>T 504
                          |||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>|
1602R-2.in silico     481 AACACCCGTGCCTTTGG<span class="snp"><tt>A</tt></span>A<span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span>T 504

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_136447.2	CG1602-RA (CG1602), mRNA
0	0	2	4	2	NM_141415.1	CG15177-RA (CG15177), mRNA
0	0	2	3	3	NM_078570.2	CG1763-RA (nod), mRNA
0	0	2	2	2	NM_057525.2	CG7937-RA (C15), mRNA
0	0	1	3	3	NM_130518.2	CG3703-RA (CG3703), mRNA
0	0	1	2	5	NM_143087.1	CG13652-RA (CG13652), mRNA
0	0	1	2	4	NM_001031863.1	CG33950-RD, transcript variant D (trol), mRNA
0	0	1	2	4	NM_001031866.1	CG33950-RA, transcript variant A (trol), mRNA
0	0	1	2	4	NM_001031864.1	CG33950-RE, transcript variant E (trol), mRNA
0	0	1	2	4	NM_001031862.1	CG33950-RC, transcript variant C (trol), mRNA
0	0	1	2	4	NM_001031867.1	CG33950-RF, transcript variant F (trol), mRNA
0	0	1	2	4	NM_140763.1	CG7408-RB (CG7408), mRNA
0	0	0	6	2	NM_136099.2	CG17344-RA (CG17344), mRNA
0	0	0	4	6	NM_141639.1	CG16779-RA (CG16779), mRNA
0	0	0	4	4	NM_206496.1	CG14869-RB, transcript variant B (CG14869), mRNA
0	0	0	4	4	NM_169659.2	CG14869-RA, transcript variant A (CG14869), mRNA
0	0	0	4	2	NM_206480.2	CG17207-RA (CG17207), mRNA
0	0	0	4	2	NM_079969.2	CG9244-RB (Acon), mRNA
0	0	0	3	10	NM_001014624.1	CG33555-RC, transcript variant C (btsz), mRNA
0	0	0	3	8	NM_141982.2	CG7242-RA (CG7242), mRNA
0	0	0	3	5	NM_165196.1	CG31739-RA (CG31739), mRNA
0	0	0	3	5	NM_057663.3	CG17161-RA, transcript variant A (grp), mRNA
0	0	0	3	4	NM_135894.2	CG15270-RA, transcript variant A (CG15270), mRNA
0	0	0	3	4	NM_205997.1	CG15270-RB, transcript variant B (CG15270), mRNA
0	0	0	3	4	NM_169057.1	CG31547-RB, transcript variant B (CG31547), mRNA
0	0	0	3	4	NM_169058.1	CG31547-RA, transcript variant A (CG31547), mRNA
0	0	0	3	4	NM_137977.2	CG11299-RA (CG11299), mRNA
0	0	0	3	4	NM_167189.2	CG32705-RA (CG32705), mRNA
0	0	0	3	3	NM_140343.2	CG17667-RA, transcript variant A (CG17667), mRNA
0	0	0	3	3	NM_168516.1	CG17667-RB, transcript variant B (CG17667), mRNA

Wing Disc

GFP

Puc

Caspase

DAPI

Merge

Figure

JNK signal

Caspase-3 signal

Dcompartment		Penetrance

Pathways (updated: 2024/04/26)

KEGG Pathway

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