NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

15822R-1

Symbol

CG15822

Full Name

CG15822

CG No

CG15822

Old CG No

CG15822

Synonyms

CG15822

Accession No (Link to NCBI)

NM_139436.1

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Fan W, Lam SM, Xin J, Yang X, Liu Z, Liu Y, Wang Y, Shui G, Huang X.<br> Drosophila TRF2 and TAF9 regulate lipid droplet size and phospholipid fatty acid composition.<br> PLoS Genet (2017) 13(3) e1006664 [ PubMed ID = 28273089 ] [ RRC reference ]

Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.<br> Zyxin links fat signaling to the hippo pathway.<br> PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAGACGCGGAGTGCCTACTTAT

Primer Seq. 3'

aatctagaggtaccCTCCATCGAAGCCAAAGTCTG

Predicted Fragment Size

IR fragment full Seq

0001 AGACGCGGAG TGCCTACTTA TCCGGCGGAT TCGATCGCCA GAANGCGCAT CGTCTTCGTT 
0061 GTCAACNGCA TTCAACGATC TGCAGCTGTG GAATCGCCGC TATCTGCAGG TGACTCTGGA 
0121 CTATCTGAAG CGGTCTCTCA GCGCTTCGGT GTTGCAGAAT GGCGTGAGTG TGGTGGTCAA 
0181 TGCCCAGGAG AGCAGTTCGC GGATATCACG GCAGCAGGTG CGACAGATTT ACGCCCTCTT 
0241 CGGGGGCGAC NATCAATGTG GATCTGTATC TAGTCAGGGC GGAGGGCTTC TGGGAGAAGC 
0301 ATGTGGAGCC GTGCACCAAG TCCCAGGTCA AGGGAGAGCC CCTGGTGCTG TCGAAGGCGA 
0361 GGCTGTTCAA GTTCATAGAG CCGCAGAACC TGCCCGAGGA ACTGGGCGGC ACCTTGCAGT 
0421 TCAACTACGA TCTGTGGCTG CAGCAGCGCA AGTCNCATCG ACGAGTTCAC CAAGTCGCAT 
0481 GTGCAGACTT TGGCTTCGAT GGAG

in silico PCR Fragment

0001 AGACGCGGAG TGCCTACTTA TCCGGCGGAT TCGATCGCCA GAA-GCGCAT CGTCTTCGTT 
0061 GTCAAC-GCA TTCAACGATC TGCAGCTGTG GAATCGCCGC TATCTGCAGG TGACTCTGGA 
0121 CTATCTGAAG CGGTCTCTCA GCGCTTCGGT GTTGCAGAAT GGCGTGAGTG TGGTGGTCAA 
0181 TGCCCAGGAG AGCAGTTCGC GGATATCACG GCAGCAGGTG CGACAGATTT ACGCCCTCTT 
0241 CGGGGGCGAC -ATCAATGTG GATCTGTATC TAGTCAGGGC GGAGGGCTTC TGGGAGAAGC 
0301 ATGTGGAGCC GTGCACCAAG TCCCAGGTCA AGGGAGAGCC CCTGGTGCTG TCGAAGGCGA 
0361 GGCTGTTCAA GTTCATAGAG CCGCAGAACC TGCCCGAGGA ACTGGGCGGC ACCTTGCAGT 
0421 TCAACTACGA TCTGTGGCTG CAGCAGCGCA AGTC-CATCG ACGAGTTCAC CAAGTCGCAT 
0481 GTGCAGACTT TGGCTTCGAT GGAG

Assemble Data

<PRE>

15822R-1.IR_full       1   AGACGCGGAGTGCCTACTTATCCGGCGGATTCGATCGCCAGAA<span class="snp"><tt>N</tt></span>GCGCATCGTCTTCGTT 60
                           |||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||
15822R-1.in silico     1   AGACGCGGAGTGCCTACTTATCCGGCGGATTCGATCGCCAGAA<span class="snp"><tt>-</tt></span>GCGCATCGTCTTCGTT 60


15822R-1.IR_full       61  GTCAAC<span class="snp"><tt>N</tt></span>GCATTCAACGATCTGCAGCTGTGGAATCGCCGCTATCTGCAGGTGACTCTGGA 120
                           ||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||
15822R-1.in silico     61  GTCAAC<span class="snp"><tt>-</tt></span>GCATTCAACGATCTGCAGCTGTGGAATCGCCGCTATCTGCAGGTGACTCTGGA 120


15822R-1.IR_full       121 CTATCTGAAGCGGTCTCTCAGCGCTTCGGTGTTGCAGAATGGCGTGAGTGTGGTGGTCAA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15822R-1.in silico     121 CTATCTGAAGCGGTCTCTCAGCGCTTCGGTGTTGCAGAATGGCGTGAGTGTGGTGGTCAA 180


15822R-1.IR_full       181 TGCCCAGGAGAGCAGTTCGCGGATATCACGGCAGCAGGTGCGACAGATTTACGCCCTCTT 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15822R-1.in silico     181 TGCCCAGGAGAGCAGTTCGCGGATATCACGGCAGCAGGTGCGACAGATTTACGCCCTCTT 240


15822R-1.IR_full       241 CGGGGGCGAC<span class="snp"><tt>N</tt></span>ATCAATGTGGATCTGTATCTAGTCAGGGCGGAGGGCTTCTGGGAGAAGC 300
                           ||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||
15822R-1.in silico     241 CGGGGGCGAC<span class="snp"><tt>-</tt></span>ATCAATGTGGATCTGTATCTAGTCAGGGCGGAGGGCTTCTGGGAGAAGC 300


15822R-1.IR_full       301 ATGTGGAGCCGTGCACCAAGTCCCAGGTCAAGGGAGAGCCCCTGGTGCTGTCGAAGGCGA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15822R-1.in silico     301 ATGTGGAGCCGTGCACCAAGTCCCAGGTCAAGGGAGAGCCCCTGGTGCTGTCGAAGGCGA 360


15822R-1.IR_full       361 GGCTGTTCAAGTTCATAGAGCCGCAGAACCTGCCCGAGGAACTGGGCGGCACCTTGCAGT 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15822R-1.in silico     361 GGCTGTTCAAGTTCATAGAGCCGCAGAACCTGCCCGAGGAACTGGGCGGCACCTTGCAGT 420


15822R-1.IR_full       421 TCAACTACGATCTGTGGCTGCAGCAGCGCAAGTC<span class="snp"><tt>N</tt></span>CATCGACGAGTTCACCAAGTCGCAT 480
                           ||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||
15822R-1.in silico     421 TCAACTACGATCTGTGGCTGCAGCAGCGCAAGTC<span class="snp"><tt>-</tt></span>CATCGACGAGTTCACCAAGTCGCAT 480


15822R-1.IR_full       481 GTGCAGACTTTGGCTTCGATGGAG 504
                           ||||||||||||||||||||||||
15822R-1.in silico     481 GTGCAGACTTTGGCTTCGATGGAG 504

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_139436.1	CG15822-RC (CG15822), mRNA
0	0	2	5	2	NM_141583.2	CG9836-RA (CG9836), mRNA
0	0	2	4	4	NM_133090.1	CG6696-RA (CG6696), mRNA
0	0	2	3	10	NM_167521.2	CG32575-RA, transcript variant A (hang), mRNA
0	0	2	3	9	NM_135751.2	CG5075-RA (CG5075), mRNA
0	0	2	3	2	NM_142583.2	CG4703-RA (Arc42), mRNA
0	0	2	2	2	NM_139491.1	CG1241-RA (Atg2), mRNA
0	0	1	8	4	NM_176385.1	CG33214-RA (CG33214), mRNA
0	0	1	3	7	NM_139528.2	CG17569-RB, transcript variant B (gry), mRNA
0	0	1	3	7	NM_168000.1	CG17569-RA, transcript variant A (gry), mRNA
0	0	1	3	5	NM_079277.2	CG3705-RA (aay), mRNA
0	0	1	3	3	NM_135795.1	CG9395-RA (CG9395), mRNA
0	0	1	3	2	NM_142103.1	CG14846-RA (CG14846), mRNA
0	0	1	2	6	NM_134771.2	CG18317-RA (CG18317), mRNA
0	0	1	2	6	NM_001014715.1	CG33513-RB, transcript variant B (Nmdar2), mRNA
0	0	1	2	6	NM_001014714.1	CG33513-RC, transcript variant C (Nmdar2), mRNA
0	0	1	2	6	NM_001014716.1	CG33513-RA, transcript variant A (Nmdar2), mRNA
0	0	1	2	3	NM_138007.2	CG3735-RA (CG3735), mRNA
0	0	1	2	3	NM_080016.2	CG4481-RA (Glu-RIB), mRNA
0	0	1	2	2	NM_167310.1	CG10347-RB, transcript variant B (CG10347), mRNA
0	0	1	2	2	NM_132527.1	CG10347-RA, transcript variant A (CG10347), mRNA
0	0	1	2	2	NM_164480.1	CG4272-RB, transcript variant B (CG4272), mRNA
0	0	1	2	2	NM_134829.2	CG4272-RA, transcript variant A (CG4272), mRNA
0	0	0	6	2	NM_137039.1	CG13332-RA (CG13332), mRNA
0	0	0	5	14	NM_168103.1	CG7507-RB, transcript variant B (Dhc64C), mRNA
0	0	0	5	14	NM_079205.3	CG7507-RA, transcript variant A (Dhc64C), mRNA
0	0	0	5	9	NM_138220.1	CG13889-RA (CG13889), mRNA
0	0	0	5	3	NM_138049.2	CG3328-RA (CG3328), mRNA
0	0	0	4	7	NM_143462.2	CG1969-RA, transcript variant A (CG1969), mRNA
0	0	0	4	7	NM_170422.1	CG1969-RB, transcript variant B (CG1969), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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