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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

15255R-2

Symbol

CG15255

Full Name

CG15255

CG No

CG15255

Old CG No

CG15255

Synonyms

BG:BACR44L22.1, CG15255

Accession No (Link to NCBI)

NM_135911.1

Inserted Chr.

Insertional Mutation

lethal

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAGGTGGATTTGTCGAGGGTGA

Primer Seq. 3'

aatctagaggtaccCTGTGTCGGCATACTTTTGGA

Predicted Fragment Size

IR fragment full Seq

0001 AGGTGGATTT GTCGAGGGTG ATATGATGCT AACGGAGGAG CAACAAAGAA ATCTGGAGCA 
0061 GGGTGCTCCC AAGGCTCGCA ATGGCCTTAT CAACACGGAA AAGAGATGGC CTGGAAATGT 
0121 GGTGGTCTAC AGGATATCAG ATGACTTTGA CACGGCGCAC AAGAAGGCCA TCCAAACTGG 
0181 CATCGATACC TTGGAGCTGC ACACTTGTTT GAGGTTTAGG GAAGCCACCG ATGAGGATAA 
0241 GGCCTATTTG ACGGTTACGG CCAAGTCTGG AGGATGCTAC ACTGCCGTCG GCTACCAAGG 
0301 TGCCCCGCAG GAAATGAATC TGGAAATATA CCCGCTCGGC GAAGGCTGTT TCCGGCCCGG 
0361 AACGATCCTG CATGAGTTTA TGCACGCCTT GGGATTCTAT CACCAACAGA GCTCGTCCAT 
0421 TCGAGATGAT TTTATAAACG TGATCTACGA AAACATAGTG CCGGGCAAGG AATTCAATTT 
0481 CCAAAAGTAT GCCGACACAG

in silico PCR Fragment

0001 AGGTGGATTT GTCGAGGGTG ATATGATGCT AACGGAGGAG CAACAAAGAA ATCTGGAGCA 
0061 GGGTGCTCCC AAGGCTCGCA ATGGCCTTAT CAACACGGAA AAGAGATGGC CTGGAAATGT 
0121 GGTGGTCTAC AGGATATCAG ATGACTTTGA CACGGCGCAC AAGAAGGCCA TCCAAACTGG 
0181 CATCGATACC TTGGAGCTGC ACACTTGTTT GAGGTTTAGG GAAGCCACCG ATGAGGATAA 
0241 GGCCTATTTG ACGGTTACGG CCAAGTCTGG AGGATGCTAC ACTGCCGTCG GCTACCAAGG 
0301 TGCCCCGCAG GAAATGAATC TGGAAATATA CCCGCTCGGC GAAGGCTGTT TCCGGCCCGG 
0361 AACGATCCTG CATGAGTTTA TGCACGCCTT GGGATTCTAT CACCAACAGA GCTCGTCCAT 
0421 TCGAGATGAT TTTATAAACG TGATCTACGA AAACATAGTG CCGGGCAAGG AATTCAATTT 
0481 CCAAAAGTAT GCCGACACAG

Assemble Data


15255R-2.IR_full       1   AGGTGGATTTGTCGAGGGTGATATGATGCTAACGGAGGAGCAACAAAGAAATCTGGAGCA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15255R-2.in silico     1   AGGTGGATTTGTCGAGGGTGATATGATGCTAACGGAGGAGCAACAAAGAAATCTGGAGCA 60


15255R-2.IR_full       61  GGGTGCTCCCAAGGCTCGCAATGGCCTTATCAACACGGAAAAGAGATGGCCTGGAAATGT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15255R-2.in silico     61  GGGTGCTCCCAAGGCTCGCAATGGCCTTATCAACACGGAAAAGAGATGGCCTGGAAATGT 120


15255R-2.IR_full       121 GGTGGTCTACAGGATATCAGATGACTTTGACACGGCGCACAAGAAGGCCATCCAAACTGG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15255R-2.in silico     121 GGTGGTCTACAGGATATCAGATGACTTTGACACGGCGCACAAGAAGGCCATCCAAACTGG 180


15255R-2.IR_full       181 CATCGATACCTTGGAGCTGCACACTTGTTTGAGGTTTAGGGAAGCCACCGATGAGGATAA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15255R-2.in silico     181 CATCGATACCTTGGAGCTGCACACTTGTTTGAGGTTTAGGGAAGCCACCGATGAGGATAA 240


15255R-2.IR_full       241 GGCCTATTTGACGGTTACGGCCAAGTCTGGAGGATGCTACACTGCCGTCGGCTACCAAGG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15255R-2.in silico     241 GGCCTATTTGACGGTTACGGCCAAGTCTGGAGGATGCTACACTGCCGTCGGCTACCAAGG 300


15255R-2.IR_full       301 TGCCCCGCAGGAAATGAATCTGGAAATATACCCGCTCGGCGAAGGCTGTTTCCGGCCCGG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15255R-2.in silico     301 TGCCCCGCAGGAAATGAATCTGGAAATATACCCGCTCGGCGAAGGCTGTTTCCGGCCCGG 360


15255R-2.IR_full       361 AACGATCCTGCATGAGTTTATGCACGCCTTGGGATTCTATCACCAACAGAGCTCGTCCAT 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15255R-2.in silico     361 AACGATCCTGCATGAGTTTATGCACGCCTTGGGATTCTATCACCAACAGAGCTCGTCCAT 420


15255R-2.IR_full       421 TCGAGATGATTTTATAAACGTGATCTACGAAAACATAGTGCCGGGCAAGGAATTCAATTT 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15255R-2.in silico     421 TCGAGATGATTTTATAAACGTGATCTACGAAAACATAGTGCCGGGCAAGGAATTCAATTT 480


15255R-2.IR_full       481 CCAAAAGTATGCCGACACAG 500
                           ||||||||||||||||||||
15255R-2.in silico     481 CCAAAAGTATGCCGACACAG 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_135911.1	CG15255-RA (CG15255), mRNA
0.2	1	2	2	3	NM_135191.2	CG9537-RA (DLP), mRNA
0	0	5	4	3	NM_134671.1	CG11592-RA (CG11592), mRNA
0	0	5	3	4	NM_135691.1	CG6734-RA (CG6734), mRNA
0	0	3	2	2	NM_001014500.1	CG33558-RA (CG33558), mRNA
0	0	2	2	3	NM_167704.1	CG11710-RA, transcript variant A (CG11710), mRNA
0	0	2	2	3	NM_134549.2	CG11710-RB, transcript variant B (CG11710), mRNA
0	0	1	5	3	NM_078697.2	CG1668-RA, transcript variant A (Pbprp2), mRNA
0	0	1	5	3	NM_176767.1	CG1668-RB, transcript variant B (Pbprp2), mRNA
0	0	1	3	3	NM_057675.3	CG8169-RA (Pms2), mRNA
0	0	1	2	8	NM_134281.1	CG8651-RC, transcript variant C (trx), mRNA
0	0	1	2	8	NM_057421.2	CG8651-RD, transcript variant D (trx), mRNA
0	0	1	2	8	NM_057422.2	CG8651-RB, transcript variant B (trx), mRNA
0	0	1	2	8	NM_001014621.1	CG8651-RE, transcript variant E (trx), mRNA
0	0	1	2	8	NM_134282.1	CG8651-RA, transcript variant A (trx), mRNA
0	0	1	2	7	NM_165226.1	CG7100-RF, transcript variant F (CadN), mRNA
0	0	1	2	7	NM_165227.1	CG7100-RD, transcript variant D (CadN), mRNA
0	0	1	2	7	NM_165228.1	CG7100-RC, transcript variant C (CadN), mRNA
0	0	1	2	7	NM_165229.1	CG7100-RA, transcript variant A (CadN), mRNA
0	0	1	2	7	NM_165230.1	CG7100-RG, transcript variant G (CadN), mRNA
0	0	1	2	7	NM_165231.1	CG7100-RE, transcript variant E (CadN), mRNA
0	0	1	2	7	NM_001032108.1	CG7100-RJ, transcript variant J (CadN), mRNA
0	0	1	2	7	NM_001032109.1	CG7100-RI, transcript variant I (CadN), mRNA
0	0	1	2	7	NM_001032107.1	CG7100-RK, transcript variant K (CadN), mRNA
0	0	1	2	7	NM_001032106.1	CG7100-RL, transcript variant L (CadN), mRNA
0	0	1	2	7	NM_165225.1	CG7100-RB, transcript variant B (CadN), mRNA
0	0	1	2	7	NM_165224.1	CG7100-RH, transcript variant H (CadN), mRNA
0	0	0	4	7	NM_142483.2	CG7702-RA, transcript variant A (CG7702), mRNA
0	0	0	4	7	NM_169829.1	CG7702-RB, transcript variant B (CG7702), mRNA
0	0	0	4	5	NM_139511.2	CG1869-RA (CG1869), mRNA

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