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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 15253R-1

Symbol CG15253 Full Name CG15253

CG No CG15253 Old CG No CG15253

Synonyms BACR44L22.3, BG:BACR44L22.3, CG15253

Accession No (Link to NCBI) NM_135914.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGAGGGTGATATGGTGCCCAGT

Primer Seq. 3'

aatctagaggtaccTGCATTACGCTGCCATAGTCG

Predicted Fragment Size

IR fragment full Seq

0001 GAGGGTAGAT ATGGTGCCCA GTGGATCCTC GAGAAATATT TGGCGCAACG AAACCTATAG 
0061 ATGGCCCAAT CGCATTATTT ATTACCATAT TAATAGCTAT ATTGATGAAG AACATCGTAA 
0121 CCACATCGTG AGCGCTATTC AGAAAATCGA ATCCATTTCA TGCCTGACCT TCAAAGAGGC 
0181 CACCACTGAT CAGAAGTATT ATGTAAATGT GACCTCCGAG GAGGGCGGCT GTTTCTCCTA 
0241 CATCGGTTAT CTGAATCGCG TTCAACAACT GAATCTCCAG AATAATGAAA TAGGCGTCGG 
0301 TTGTTTCCGT TTGTATACGA TAGTGCATGA GTTCCTGCAT GCCCTTGGAT TTTTCCATCA 
0361 GCAAAGTGCC GCCGATCGGG ATGATTATGT CCAAATTGTG GAGGAGAATA TCACCGAAGG 
0421 CATGGAGTTT AACTTTGATA AATATACCGA GGAAACCGTC AATGATTTTG GTGAGAAATA 
0481 CGACTATGGC AGCGTAATGC A

in silico PCR Fragment

0001 GAGGGT-GAT ATGGTGCCCA GTGGATCCTC GAGAAATATT TGGCGCAACG AAACCTATAG 
0061 ATGGCCCAAT CGCATTATTT ATTACCATAT TAATAGCTAT ATTGATGAAG AACATCGTAA 
0121 CCACATCGTG AGCGCTATTC AGAAAATCGA ATCCATTTCA TGCCTGACCT TCAAAGAGGC 
0181 CACCACTGAT CAGAAGTATT ATGTAAATGT GACCTCCGAG GAGGGCGGTT GTTTCTCCTA 
0241 CATCGGTTAT CTGAATCGCG TTCAACAACT GAATCTCCAG AATAATGAAA TAGGCGTCGG 
0301 TTGTTTCCGT TTGTATACGA TAGTGCATGA GTTCCTGCAT GCCCTTGGAT TTTTCCATCA 
0361 GCAAAGTGCC GCCGATCGGG ATGATTATGT CCAAATTGTG GAGGAGAATA TCACCGAAGG 
0421 CATGGAGTTT AACTTTGATA AATATACCGA GGAAACCGTC AATGATTTTG GTGAGAAATA 
0481 CGACTATGGC AGCGTAATGC A

Assemble Data


15253R-1.IR_full       1   GAGGGTAGATATGGTGCCCAGTGGATCCTCGAGAAATATTTGGCGCAACGAAACCTATAG 60
                           |||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||
15253R-1.in silico     1   GAGGGT-GATATGGTGCCCAGTGGATCCTCGAGAAATATTTGGCGCAACGAAACCTATAG 60


15253R-1.IR_full       61  ATGGCCCAATCGCATTATTTATTACCATATTAATAGCTATATTGATGAAGAACATCGTAA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15253R-1.in silico     61  ATGGCCCAATCGCATTATTTATTACCATATTAATAGCTATATTGATGAAGAACATCGTAA 120


15253R-1.IR_full       121 CCACATCGTGAGCGCTATTCAGAAAATCGAATCCATTTCATGCCTGACCTTCAAAGAGGC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15253R-1.in silico     121 CCACATCGTGAGCGCTATTCAGAAAATCGAATCCATTTCATGCCTGACCTTCAAAGAGGC 180


15253R-1.IR_full       181 CACCACTGATCAGAAGTATTATGTAAATGTGACCTCCGAGGAGGGCGGCTGTTTCTCCTA 240
                           |||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||
15253R-1.in silico     181 CACCACTGATCAGAAGTATTATGTAAATGTGACCTCCGAGGAGGGCGGTTGTTTCTCCTA 240


15253R-1.IR_full       241 CATCGGTTATCTGAATCGCGTTCAACAACTGAATCTCCAGAATAATGAAATAGGCGTCGG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15253R-1.in silico     241 CATCGGTTATCTGAATCGCGTTCAACAACTGAATCTCCAGAATAATGAAATAGGCGTCGG 300


15253R-1.IR_full       301 TTGTTTCCGTTTGTATACGATAGTGCATGAGTTCCTGCATGCCCTTGGATTTTTCCATCA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15253R-1.in silico     301 TTGTTTCCGTTTGTATACGATAGTGCATGAGTTCCTGCATGCCCTTGGATTTTTCCATCA 360


15253R-1.IR_full       361 GCAAAGTGCCGCCGATCGGGATGATTATGTCCAAATTGTGGAGGAGAATATCACCGAAGG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15253R-1.in silico     361 GCAAAGTGCCGCCGATCGGGATGATTATGTCCAAATTGTGGAGGAGAATATCACCGAAGG 420


15253R-1.IR_full       421 CATGGAGTTTAACTTTGATAAATATACCGAGGAAACCGTCAATGATTTTGGTGAGAAATA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15253R-1.in silico     421 CATGGAGTTTAACTTTGATAAATATACCGAGGAAACCGTCAATGATTTTGGTGAGAAATA 480


15253R-1.IR_full       481 CGACTATGGCAGCGTAATGCA 501
                           |||||||||||||||||||||
15253R-1.in silico     481 CGACTATGGCAGCGTAATGCA 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_135914.1	CG15253-RA (CG15253), mRNA
0.41	2	15	18	49	NM_135913.2	CG15254-RA (CG15254), mRNA
0	0	5	2	3	NM_142881.2	CG6763-RA (CG6763), mRNA
0	0	4	2	6	NM_079876.2	CG1483-RA, transcript variant A (Map205), mRNA
0	0	4	2	6	NM_170586.1	CG1483-RB, transcript variant B (Map205), mRNA
0	0	1	10	3	NM_142985.1	CG5715-RA (CG5715), mRNA
0	0	1	3	2	NM_137095.2	CG8415-RA (RpS23), mRNA
0	0	1	3	2	NM_142083.2	CG14355-RA (CG14355), mRNA
0	0	1	2	9	NM_079132.2	CG16910-RA (key), mRNA
0	0	1	2	5	NM_138124.2	CG3589-RA (CG3589), mRNA
0	0	1	2	5	NM_137497.2	CG10924-RA (CG10924), mRNA
0	0	1	2	3	NM_141894.2	CG14739-RA (CG14739), mRNA
0	0	1	2	3	NM_137232.3	CG8389-RB, transcript variant B (CG8389), mRNA
0	0	1	2	3	NM_166132.2	CG8389-RA, transcript variant A (CG8389), mRNA
0	0	1	2	3	NM_168346.1	CG32043-RB, transcript variant B (CG32043), mRNA
0	0	1	2	3	NM_140064.2	CG32043-RA, transcript variant A (CG32043), mRNA
0	0	1	2	2	NM_139758.1	CG10475-RA (Jon65Ai), mRNA
0	0	0	6	2	NM_078517.2	CG2175-RA, transcript variant A (dec-1), mRNA
0	0	0	6	2	NM_167133.1	CG2175-RC, transcript variant C (dec-1), mRNA
0	0	0	6	2	NM_167132.1	CG2175-RB, transcript variant B (dec-1), mRNA
0	0	0	5	2	NM_143065.2	CG6422-RA, transcript variant A (CG6422), mRNA
0	0	0	5	2	NM_170188.1	CG6422-RB, transcript variant B (CG6422), mRNA
0	0	0	4	3	NM_057949.3	CG10270-RA (D19B), mRNA
0	0	0	3	6	NM_135344.2	CG8486-RA, transcript variant A (CG8486), mRNA
0	0	0	3	6	NM_164795.2	CG8486-RB, transcript variant B (CG8486), mRNA
0	0	0	3	6	NM_001042881.1	CG8486-RC, transcript variant C (CG8486), mRNA
0	0	0	3	5	NM_164517.1	CG8817-RC, transcript variant C (lilli), mRNA
0	0	0	3	5	NM_164516.1	CG8817-RA, transcript variant A (lilli), mRNA
0	0	0	3	5	NM_078741.2	CG8825-RA (gkt), mRNA
0	0	0	3	5	NM_142098.1	CG8538-RA (CG8538), mRNA

Wing Disc

GFP

Puc

Caspase

DAPI

Merge

Figure

JNK signal

Caspase-3 signal

Dcompartment		Penetrance

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