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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

15021R-1

Symbol

CG15021

Full Name

CG15021

CG No

CG15021

Old CG No

CG15021

Synonyms

CT34875, CG15021

Accession No (Link to NCBI)

NM_139645.2

Inserted Chr.

Insertional Mutation

lethal

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTCCATCCTATGGTCCTCCGCA

Primer Seq. 3'

aatctagaggtaccTCTCTCCAGGAGGTGGCAAAT

Predicted Fragment Size

IR fragment full Seq

0001 TCCATCCTAT GGTCCTCCGC AGACGCCTCC ACCACGTCCT CCACCACAGC CAACTCCATC 
0061 GGCTCCCGCT CCACCACCAT CTTATGGACC GCCGCAGACA CCACCACCTC GTCCTCCACC 
0121 GCAGCCAACA CCTTCGGCTC CAGCTCCATC CTATGGACCT CCTCAGCCTC AGCCACCGGC 
0181 ACCACAGCCA CCATCGCCAC CATCGCCACA ACCTGGGCCA GAGTACCTGC CTCCAGATCA 
0241 GCCAAAGCCA CGTCCAACGC CCTCGCGTCC TCAGCCGCCT CCACCACCAC CACCTAGGCC 
0301 CCAGCCAACT CCTGGCTACG GACCACCACC GCCACCACCT CCGCCTAAGC CACAGCCTAC 
0361 TCCAGGATAC GGCCCTCCTA CACCACCACC AGGACCAGGA CCCGCTCCGC CGGCACCACA 
0421 GCCGCCAAGA CCACAGCCGC CACGCCCACA GCCACCACGG CCACAGCCAG GATCTGAGTA 
0481 TTTGCCACCT CCTGGAGAGA

in silico PCR Fragment

0001 TCCATCCTAT GGTCCTCCGC AGACGCCTCC ACCACGTCCT CCACCACAGC CAACTCCATC 
0061 GGCTCCCGCT CCACCACCAT CTTATGGACC GCCGCAGACA CCACCACCTC GTCCTCCACC 
0121 GCAGCCAACA CCTTCGGCTC CAGCTCCATC CTATGGACCT CCTCAGCCTC AGCCACCGGC 
0181 ACCACAGCCA CCATCGCCAC CATCGCCACA ACCTGGGCCA GAGTACCTGC CTCCAGATCA 
0241 GCCAAAGCCA CGTCCAACGC CCTCGCGTCC TCAGCCGCCT CCACCACCAC CACCTAGGCC 
0301 CCAGCCAACT CCTGGCTACG GACCACCACC GCCACCACCT CCGCCTAAGC CACAGCCTAC 
0361 TCCAGGATAC GGCCCTCCTA CACCACCACC AGGACCAGGA CCCGCTCAGC CGGCACCACA 
0421 GCCGCCAAGA CCACAGCCGC CACGCCCACA GCCACCACGG CCACAGCCAG GATCTGAGTA 
0481 TTTGCCACCT CCTGGAGAGA

Assemble Data

<PRE>

15021R-1.IR_full       1   TCCATCCTATGGTCCTCCGCAGACGCCTCCACCACGTCCTCCACCACAGCCAACTCCATC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15021R-1.in silico     1   TCCATCCTATGGTCCTCCGCAGACGCCTCCACCACGTCCTCCACCACAGCCAACTCCATC 60


15021R-1.IR_full       61  GGCTCCCGCTCCACCACCATCTTATGGACCGCCGCAGACACCACCACCTCGTCCTCCACC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15021R-1.in silico     61  GGCTCCCGCTCCACCACCATCTTATGGACCGCCGCAGACACCACCACCTCGTCCTCCACC 120


15021R-1.IR_full       121 GCAGCCAACACCTTCGGCTCCAGCTCCATCCTATGGACCTCCTCAGCCTCAGCCACCGGC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15021R-1.in silico     121 GCAGCCAACACCTTCGGCTCCAGCTCCATCCTATGGACCTCCTCAGCCTCAGCCACCGGC 180


15021R-1.IR_full       181 ACCACAGCCACCATCGCCACCATCGCCACAACCTGGGCCAGAGTACCTGCCTCCAGATCA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15021R-1.in silico     181 ACCACAGCCACCATCGCCACCATCGCCACAACCTGGGCCAGAGTACCTGCCTCCAGATCA 240


15021R-1.IR_full       241 GCCAAAGCCACGTCCAACGCCCTCGCGTCCTCAGCCGCCTCCACCACCACCACCTAGGCC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15021R-1.in silico     241 GCCAAAGCCACGTCCAACGCCCTCGCGTCCTCAGCCGCCTCCACCACCACCACCTAGGCC 300


15021R-1.IR_full       301 CCAGCCAACTCCTGGCTACGGACCACCACCGCCACCACCTCCGCCTAAGCCACAGCCTAC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15021R-1.in silico     301 CCAGCCAACTCCTGGCTACGGACCACCACCGCCACCACCTCCGCCTAAGCCACAGCCTAC 360


15021R-1.IR_full       361 TCCAGGATACGGCCCTCCTACACCACCACCAGGACCAGGACCCGCTC<span class="snp"><tt>C</tt></span>GCCGGCACCACA 420
                           |||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||
15021R-1.in silico     361 TCCAGGATACGGCCCTCCTACACCACCACCAGGACCAGGACCCGCTC<span class="snp"><tt>A</tt></span>GCCGGCACCACA 420


15021R-1.IR_full       421 GCCGCCAAGACCACAGCCGCCACGCCCACAGCCACCACGGCCACAGCCAGGATCTGAGTA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15021R-1.in silico     421 GCCGCCAAGACCACAGCCGCCACGCCCACAGCCACCACGGCCACAGCCAGGATCTGAGTA 480


15021R-1.IR_full       481 TTTGCCACCTCCTGGAGAGA 500
                           ||||||||||||||||||||
15021R-1.in silico     481 TTTGCCACCTCCTGGAGAGA 500

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	37	136	305	NM_139645.2	CG15021-RA (CG15021), mRNA
0.82	4	9	16	34	NM_132669.1	CG11816-RA (CG11816), mRNA
0.82	4	2	13	30	NM_134474.4	CG32532-RA (CG32532), mRNA
0.62	3	10	43	186	NM_142330.1	CG5225-RA (CG5225), mRNA
0.62	3	8	8	43	NM_206154.1	CG33130-RB, transcript variant B (l(2)k07433), mRNA
0.62	3	8	8	43	NM_176218.1	CG33130-RA, transcript variant A (l(2)k07433), mRNA
0.62	3	8	8	43	NM_206153.1	CG33130-RC, transcript variant C (l(2)k07433), mRNA
0.62	3	4	5	57	NM_167776.1	CG14619-RE, transcript variant E (CG14619), mRNA
0.62	3	4	5	57	NM_167775.1	CG14619-RD, transcript variant D (CG14619), mRNA
0.62	3	4	5	57	NM_134618.2	CG14619-RA, transcript variant A (CG14619), mRNA
0.62	3	3	12	38	NM_141642.1	CG9373-RA (CG9373), mRNA
0.41	2	5	13	54	NM_001014706.1	CG13376-RA (CG13376), mRNA
0.2	1	3	9	32	NM_079195.2	CG15002-RB (mas), mRNA
0.2	1	3	6	20	NM_135130.1	CG9050-RA (CG9050), mRNA
0.2	1	2	16	46	NM_140925.2	CG7323-RA (CG7323), mRNA
0.2	1	2	16	27	NM_134527.1	CG15322-RA (CG15322), mRNA
0.2	1	2	5	18	NM_164429.1	CG31666-RC, transcript variant C (CG31666), mRNA
0.2	1	2	5	18	NM_164428.2	CG31666-RB, transcript variant B (CG31666), mRNA
0.2	1	2	5	18	NM_134766.3	CG31666-RA, transcript variant A (CG31666), mRNA
0.2	1	2	5	18	NM_164430.2	CG31666-RD, transcript variant D (CG31666), mRNA
0.2	1	2	4	16	NM_080365.2	CG4894-RB, transcript variant B (Ca-alpha1D), mRNA
0.2	1	2	4	16	NM_165146.1	CG4894-RC, transcript variant C (Ca-alpha1D), mRNA
0.2	1	2	4	16	NM_165147.1	CG4894-RD, transcript variant D (Ca-alpha1D), mRNA
0.2	1	2	4	16	NM_134429.2	CG4894-RA, transcript variant A (Ca-alpha1D), mRNA
0	0	11	32	95	NM_164565.1	CG3399-RC, transcript variant C (capu), mRNA
0	0	11	32	95	NM_164566.1	CG3399-RD, transcript variant D (capu), mRNA
0	0	11	32	95	NM_164567.1	CG3399-RB, transcript variant B (capu), mRNA
0	0	11	32	95	NM_057618.2	CG3399-RA, transcript variant A (capu), mRNA
0	0	9	8	83	NM_130680.2	CG3588-RB, transcript variant B (CG3588), mRNA
0	0	8	23	115	NM_141397.2	CG1021-RB, transcript variant B (CG1021), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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