NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

14997R-2

Symbol

CG14997

Full Name

CG14997

CG No

CG14997

Old CG No

CG14997

Synonyms

BcDNA:GH04863, CG14997

Accession No (Link to NCBI)

NM_139620.1

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATGAACCGTCGCCTTCCAGGA

Primer Seq. 3'

aatctagaggtaccTTACTGTCGGGGGTCTCCAGG

Predicted Fragment Size

IR fragment full Seq

0001 ATGAACCGTC GCCTTCCAGG AACCATCAGC CAGTGCCAGA AGCTTCTCCT AGCCACCCAC 
0061 AACCATGCTC CAACGGCCTT CTCCACCAGA TTCCTGTCCA CATCTCGAGT GAACCGCGAG 
0121 CGGCAAGAAT GCCAAGTGCT GGTTGTGGGC GGTGGCACCG GTGGGTGTGC CATGGCCGCC 
0181 AAGTTGTCCT CACGTTTGGG CAGCGACAAG GTGGTCGTCC TGGAACCGGA AGATAAACAT 
0241 TACTACCAAC CCATGTTCAC CCTAATAGGA GGTGGTATGA AGAGATTGGA TCAGAGCCAC 
0301 CGCCAAATGG CCGATGTGCT GCCCACAAAG GCCAAATGGG TGAAGGAGAA GGCGCTGGAG 
0361 TTCGATCCGG ATAATAATAC GGTTAGCACT TCTGGTGGCA AGACCATCAA GTACGACTTC 
0421 CTGATCATCG CCACCGGACT GCAGCTCAAT TATGGAAAGA TACCCGGCCT GGTGGAGGCC 
0481 CTGGAGACCC CCGACAGTAA

in silico PCR Fragment

0001 ATGAACCGTC GCCTTCCAGG AACCATCAGC CAGTGCCAGA AGCTTCTCCT AGCCACCCAC 
0061 AACCATGCTC CAACGGCCTT CTCCACCAGA TTCCTGTCCA CATCTCGAGT GAACCGCGAG 
0121 CGGCAAGAAT GCCAAGTGCT GGTTGTGGGC GGTGGCACCG GTGGGTGTGC CATGGCCGCC 
0181 AAGTTGTCCT CACGTTTGGG CAGCGACAAG GTGGTCGTCC TGGAACCGGA AGATAAACAT 
0241 TACTACCAAC CCATGTTCAC CCTAATAGGA GGTGGTATGA AGAGATTGGA TCAGAGCCAC 
0301 CGCCAAATGG CCGATGTGCT GCCCACAAAG GCCAAATGGG TGAAGGAGAA GGCGCTGGAG 
0361 TTCGATCCGG ATAATAATAC GGTTAGCACT TCTGGTGGCA AGACCATCAA GTACGACTTC 
0421 CTGATCATCG CCACCGGACT GCAGCTCAAT TATGGAAAGA TACCTGGCCT GGTGGAGGCC 
0481 CTGGAGACCC CCGACAGTAA

Assemble Data

<PRE>

14997R-2.IR_full       1   ATGAACCGTCGCCTTCCAGGAACCATCAGCCAGTGCCAGAAGCTTCTCCTAGCCACCCAC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14997R-2.in silico     1   ATGAACCGTCGCCTTCCAGGAACCATCAGCCAGTGCCAGAAGCTTCTCCTAGCCACCCAC 60


14997R-2.IR_full       61  AACCATGCTCCAACGGCCTTCTCCACCAGATTCCTGTCCACATCTCGAGTGAACCGCGAG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14997R-2.in silico     61  AACCATGCTCCAACGGCCTTCTCCACCAGATTCCTGTCCACATCTCGAGTGAACCGCGAG 120


14997R-2.IR_full       121 CGGCAAGAATGCCAAGTGCTGGTTGTGGGCGGTGGCACCGGTGGGTGTGCCATGGCCGCC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14997R-2.in silico     121 CGGCAAGAATGCCAAGTGCTGGTTGTGGGCGGTGGCACCGGTGGGTGTGCCATGGCCGCC 180


14997R-2.IR_full       181 AAGTTGTCCTCACGTTTGGGCAGCGACAAGGTGGTCGTCCTGGAACCGGAAGATAAACAT 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14997R-2.in silico     181 AAGTTGTCCTCACGTTTGGGCAGCGACAAGGTGGTCGTCCTGGAACCGGAAGATAAACAT 240


14997R-2.IR_full       241 TACTACCAACCCATGTTCACCCTAATAGGAGGTGGTATGAAGAGATTGGATCAGAGCCAC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14997R-2.in silico     241 TACTACCAACCCATGTTCACCCTAATAGGAGGTGGTATGAAGAGATTGGATCAGAGCCAC 300


14997R-2.IR_full       301 CGCCAAATGGCCGATGTGCTGCCCACAAAGGCCAAATGGGTGAAGGAGAAGGCGCTGGAG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14997R-2.in silico     301 CGCCAAATGGCCGATGTGCTGCCCACAAAGGCCAAATGGGTGAAGGAGAAGGCGCTGGAG 360


14997R-2.IR_full       361 TTCGATCCGGATAATAATACGGTTAGCACTTCTGGTGGCAAGACCATCAAGTACGACTTC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14997R-2.in silico     361 TTCGATCCGGATAATAATACGGTTAGCACTTCTGGTGGCAAGACCATCAAGTACGACTTC 420


14997R-2.IR_full       421 CTGATCATCGCCACCGGACTGCAGCTCAATTATGGAAAGATACC<span class="snp"><tt>C</tt></span>GGCCTGGTGGAGGCC 480
                           ||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||
14997R-2.in silico     421 CTGATCATCGCCACCGGACTGCAGCTCAATTATGGAAAGATACC<span class="snp"><tt>T</tt></span>GGCCTGGTGGAGGCC 480


14997R-2.IR_full       481 CTGGAGACCCCCGACAGTAA 500
                           ||||||||||||||||||||
14997R-2.in silico     481 CTGGAGACCCCCGACAGTAA 500

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_168071.1	CG14997-RB, transcript variant B (CG14997), mRNA
100	482	0	0	0	NM_139620.1	CG14997-RA, transcript variant A (CG14997), mRNA
0	0	3	4	4	NM_132072.1	CG14446-RA (CG14446), mRNA
0	0	3	3	5	NM_139549.1	CG11526-RB, transcript variant B (CG11526), mRNA
0	0	3	3	5	NM_168023.1	CG11526-RA, transcript variant A (CG11526), mRNA
0	0	2	5	12	NM_134961.1	CG10019-RA (CG10019), mRNA
0	0	2	2	3	NM_168743.2	CG32177-RA (CG32177), mRNA
0	0	2	2	3	NM_137119.2	CG10105-RA (CG10105), mRNA
0	0	2	2	2	NM_168210.1	CG8582-RA, transcript variant A (Sh3beta), mRNA
0	0	2	2	2	NM_139855.2	CG8582-RB, transcript variant B (Sh3beta), mRNA
0	0	1	4	3	NM_138027.2	CG11388-RA (CG11388), mRNA
0	0	1	3	2	NM_001038982.1	CG33970-RA, transcript variant A (CG33970), mRNA
0	0	1	2	12	NM_001014630.1	CG33547-RA (Rim), mRNA
0	0	1	2	5	NM_165342.3	CG9326-RB, transcript variant B (CG9326), mRNA
0	0	1	2	5	NM_165343.2	CG9326-RC, transcript variant C (CG9326), mRNA
0	0	1	2	5	NM_206011.2	CG9326-RD, transcript variant D (CG9326), mRNA
0	0	1	2	4	NM_057328.3	CG3127-RA (Pgk), mRNA
0	0	1	2	4	NM_136193.1	CG2493-RA (CG2493), mRNA
0	0	1	2	3	NM_132310.2	CG2194-RB, transcript variant B (Reg-3), mRNA
0	0	1	2	3	NM_167182.1	CG2194-RC, transcript variant C (Reg-3), mRNA
0	0	1	2	2	NM_168549.1	CG10089-RB, transcript variant B (CG10089), mRNA
0	0	1	2	2	NM_168548.1	CG10089-RA, transcript variant A (CG10089), mRNA
0	0	1	2	2	NM_140397.1	CG10089-RD, transcript variant D (CG10089), mRNA
0	0	0	6	22	NM_175960.3	CG33196-RB (dp), mRNA
0	0	0	6	5	NM_142065.1	CG9927-RA (Art6), mRNA
0	0	0	6	3	NM_140862.1	CG9452-RA (CG9452), mRNA
0	0	0	5	11	NM_205940.1	CG10595-RB, transcript variant B (d), mRNA
0	0	0	5	11	NM_205939.1	CG10595-RC, transcript variant C (d), mRNA
0	0	0	5	11	NM_175991.1	CG10595-RA, transcript variant A (d), mRNA
0	0	0	5	5	NM_136158.2	CG10538-RA (CdGAPr), mRNA