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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 14985R-1 
 Symbol CG14985  Full Name CG14985 
 CG No CG14985  Old CG No CG14985 
 Synonyms CG14985 
 Accession No (Link to NCBI) NM_139589.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		 
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTATACGCGCCGTGGAATGCGA 
 Primer Seq. 3' 
aatctagaggtaccAGACAGTCACGTTCCGCCGCT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 TATACGCGCC GTGGAATGCG ACCAAGTTGG ACGGATCTTG GANGGCGCAA ACGCTGTACA 
0061 CAGAGGGGAT CGCGCAGTTG ATGCAGTTCG TCAACGGAGA ACCGGATGAA GCCAAACGGA 
0121 AGGGGTTCCT AACCAGGATC AAGGAGTACA TGGATCGGGC TGACGCAATT AAGGCGCGGA 
0181 TCAATGGCAA ACTAATGCTC GGCGAGGTGG TTTCCCACGT TTCCATCGAC GAGAATGATA 
0241 CGGGCTTCGA CTACGATCAG CTCTTCGGGA AATACATGGA TGACAAGACC GTCGAGATCA 
0301 TGCTGGAGGA ACCCTACATG ACGCAGAACT ATCAGTATCA AAATCTAGTA AGATTCTTGG 
0361 AGCTAGCTGC CACAAATTGC CCAAACCTAA AGTATTTTCG ACTGATCACC AAAGAGTACA 
0421 AGGATGCAAA GAATCCAGAT CAGCAGGAAA CAAATCTTGG ACAGATCAAG GGTGACTTGG 
0481 AGCGGCGGAA CGTGACTGTC T 
 in silico PCR Fragment 
0001 TATACGCGCC GTGGAATGCG ACCAAGTTGG ACGGATCTTG GA-GGCGCAA ACGCTGTACA 
0061 CAGAGGGGAT CGGGCAGTTG ATGCAGTTCG TCAACGGGGA ACCGGATGAA GCCAAACGGA 
0121 AGGGGTTCCT GACCAGGATC AAGGAGTACA TGGATCGGGC TGACGCAATT AAGGCGCGGA 
0181 TCAATGGCAA ACTAATGCTC GGCGAGGTGG TTTCCCACGT TTCCATCGAC GAGAATGATA 
0241 CGGGCTTCGA CTACGATCAG CTCTTCGGGA AATACATGGA CGACAAGACC GTCGAGATCA 
0301 TGCTGGAGGA ACCCTACATG ACGCAGAACT ATCAGTATCA AAATCTAATA AGATTCTTGG 
0361 AGCTAGCTGC CACAAATTGC CCAAACCTAA AGTATTTTCG ACTGATCACC AAAGAGTACA 
0421 AGGATGCAAA GAATCCAGAT CAGCAGGAAA CAAATCTTGG ACAGATCAAG GGTGACTTGG 
0481 AGCGGCGGAA CGTGACTGTC T 
 Assemble Data 
<PRE>

14985R-1.IR_full       1   TATACGCGCCGTGGAATGCGACCAAGTTGGACGGATCTTGGA<span class="snp"><tt>N</tt></span>GGCGCAAACGCTGTACA 60
                           ||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||
14985R-1.in silico     1   TATACGCGCCGTGGAATGCGACCAAGTTGGACGGATCTTGGA<span class="snp"><tt>-</tt></span>GGCGCAAACGCTGTACA 60


14985R-1.IR_full       61  CAGAGGGGATCG<span class="snp"><tt>C</tt></span>GCAGTTGATGCAGTTCGTCAACGG<span class="snp"><tt>A</tt></span>GAACCGGATGAAGCCAAACGGA 120
                           ||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||
14985R-1.in silico     61  CAGAGGGGATCG<span class="snp"><tt>G</tt></span>GCAGTTGATGCAGTTCGTCAACGG<span class="snp"><tt>G</tt></span>GAACCGGATGAAGCCAAACGGA 120


14985R-1.IR_full       121 AGGGGTTCCT<span class="snp"><tt>A</tt></span>ACCAGGATCAAGGAGTACATGGATCGGGCTGACGCAATTAAGGCGCGGA 180
                           ||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||
14985R-1.in silico     121 AGGGGTTCCT<span class="snp"><tt>G</tt></span>ACCAGGATCAAGGAGTACATGGATCGGGCTGACGCAATTAAGGCGCGGA 180


14985R-1.IR_full       181 TCAATGGCAAACTAATGCTCGGCGAGGTGGTTTCCCACGTTTCCATCGACGAGAATGATA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14985R-1.in silico     181 TCAATGGCAAACTAATGCTCGGCGAGGTGGTTTCCCACGTTTCCATCGACGAGAATGATA 240


14985R-1.IR_full       241 CGGGCTTCGACTACGATCAGCTCTTCGGGAAATACATGGA<span class="snp"><tt>T</tt></span>GACAAGACCGTCGAGATCA 300
                           ||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||
14985R-1.in silico     241 CGGGCTTCGACTACGATCAGCTCTTCGGGAAATACATGGA<span class="snp"><tt>C</tt></span>GACAAGACCGTCGAGATCA 300


14985R-1.IR_full       301 TGCTGGAGGAACCCTACATGACGCAGAACTATCAGTATCAAAATCTA<span class="snp"><tt>G</tt></span>TAAGATTCTTGG 360
                           |||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||
14985R-1.in silico     301 TGCTGGAGGAACCCTACATGACGCAGAACTATCAGTATCAAAATCTA<span class="snp"><tt>A</tt></span>TAAGATTCTTGG 360


14985R-1.IR_full       361 AGCTAGCTGCCACAAATTGCCCAAACCTAAAGTATTTTCGACTGATCACCAAAGAGTACA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14985R-1.in silico     361 AGCTAGCTGCCACAAATTGCCCAAACCTAAAGTATTTTCGACTGATCACCAAAGAGTACA 420


14985R-1.IR_full       421 AGGATGCAAAGAATCCAGATCAGCAGGAAACAAATCTTGGACAGATCAAGGGTGACTTGG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14985R-1.in silico     421 AGGATGCAAAGAATCCAGATCAGCAGGAAACAAATCTTGGACAGATCAAGGGTGACTTGG 480


14985R-1.IR_full       481 AGCGGCGGAACGTGACTGTCT 501
                           |||||||||||||||||||||
14985R-1.in silico     481 AGCGGCGGAACGTGACTGTCT 501

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_139589.1  CG14985-RA (CG14985), mRNA 
0   0  1  4  5  NM_137042.2  CG6155-RA (Roe1), mRNA 
0   0  1  3  2  NM_131944.1  CG3546-RA (CG3546), mRNA 
0   0  1  3  2  NM_135226.2  CG11221-RA (CG11221), mRNA 
0   0  1  2  3  NM_136564.1  CG14743-RA (CG14743), mRNA 
0   0  1  2  2  NM_132967.1  CG5004-RA (CG5004), mRNA 
0   0  0  6  7  NM_168857.1  CG11796-RA, transcript variant A (CG11796), mRNA 
0   0  0  6  7  NM_140968.2  CG11796-RB, transcript variant B (CG11796), mRNA 
0   0  0  6  5  NM_167643.1  CG32537-RA (CG32537), mRNA 
0   0  0  4  13  NM_080152.2  CG5092-RA (Tor), mRNA 
0   0  0  4  6  NM_078860.2  CG4599-RA, transcript variant A (Tpr2), mRNA 
0   0  0  4  6  NM_165164.1  CG4599-RB, transcript variant B (Tpr2), mRNA 
0   0  0  4  6  NM_132121.2  CG3135-RA (shf), mRNA 
0   0  0  4  5  NM_137537.2  CG15099-RA (CG15099), mRNA 
0   0  0  4  4  NM_135390.2  CG13088-RA (CG13088), mRNA 
0   0  0  4  4  NM_169531.1  CG9412-RD, transcript variant D (rin), mRNA 
0   0  0  4  4  NM_080168.2  CG9412-RB, transcript variant B (rin), mRNA 
0   0  0  4  4  NM_169533.1  CG9412-RA, transcript variant A (rin), mRNA 
0   0  0  4  4  NM_169532.1  CG9412-RE, transcript variant E (rin), mRNA 
0   0  0  4  4  NM_169530.1  CG9412-RC, transcript variant C (rin), mRNA 
0   0  0  4  3  NM_132012.1  CG15776-RA (CG15776), mRNA 
0   0  0  4  2  NM_078807.1  CG4897-RA (RpL7), mRNA 
0   0  0  4  2  NM_135714.2  CG6579-RA (CG6579), mRNA 
0   0  0  3  9  NM_057388.2  CG9224-RA (sog), mRNA 
0   0  0  3  9  NM_057920.3  CG3423-RA (SA), mRNA 
0   0  0  3  8  NM_165022.1  CG3762-RC, transcript variant C (Vha68-2), mRNA 
0   0  0  3  8  NM_165021.1  CG3762-RB, transcript variant B (Vha68-2), mRNA 
0   0  0  3  8  NM_143747.2  CG3762-RA, transcript variant A (Vha68-2), mRNA 
0   0  0  3  6  NM_137076.1  CG6701-RA (CG6701), mRNA 
0   0  0  3  5  NM_001043023.1  CG17800-PAQ (Dscam), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway

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