NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

14968R-1

Symbol

CG14968

Full Name

CG14968

CG No

CG14968

Old CG No

CG14968

Synonyms

anon-WO0107627.3, anon-WO0107627.2, anon-WO0107627.1, CG14968

Accession No (Link to NCBI)

NM_168011.1

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGATCTGGACAGCCAGGTCAAT

Primer Seq. 3'

aatctagaggtaccTGTCGCGTGGGTGTAATAGTG

Predicted Fragment Size

IR fragment full Seq

0001 GATCTGGACA GCCAGGTCAA TGTGGAGGAT TTGCCCATAA CGTTCAAGGT GAAGTACATT 
0061 GGTTCCGAAG TGGCACGTGG CTTATGGGGC ATTAAGTATA CGCGTCGTCC GGTGGACATA 
0121 ATGGTGGGCG TGGCCAAGAA CCTGCCGCCC AATAAGGTGC TGCCCAACTG CGAACTGAAG 
0181 GTGTCCACCG ACGGAGTCCA GCTGGAGATC ATATCGCCAA AGGCCAGCAT CAATCACTGG 
0241 AGCTATCCCA TCGACACGAT CTCGTATGGC GTTCAGGACC TGGTCTACAC AAGGGTCTTT 
0301 GCCATGATCG TGGTGAAGGA CGAGTCGAGT CCGCATCCCT TTGAGGTTCA CGCCTTCGTG 
0361 TGCGACAGTC GTGCGATGGC GCGGAAATTG ACCTTTGCCC TGGcCGCCGC CTTCCAGGAT 
0421 TACTCGCGAC GGGTCAAGGA GGCAACCGGT GAGGAGGAGG GCGAGGcCAC GCCCAGCGAC 
0481 ACTATTACAC CCACGCGACA

in silico PCR Fragment

0001 GATCTGGACA GCCAGGTCAA TGTGGAGGAT TTGCCCATAA CGTTCAAGGT GAAGTACATT 
0061 GGTTCCGAAG TGGCACGTGG CTTATGGGGC ATTAAGTATA CGCGTCGTCC GGTGGACATA 
0121 ATGGTGGGCG TGGCCAAGAA CCTGCCGCCC AATAAGGTGC TGCCCAACTG CGAACTGAAG 
0181 GTGTCCACCG ACGGAGTCCA GCTGGAGATC ATATCGCCAA AGGCCAGCAT CAATCACTGG 
0241 AGCTATCCCA TCGACACGAT CTCGTATGGC GTTCAGGACC TGGTCTACAC AAGGGTCTTT 
0301 GCCATGATCG TGGTGAAGGA CGAGTCGAGT CCGCATCCCT TTGAGGTTCA CGCCTTCGTG 
0361 TGCGACAGTC GTGCGATGGC GCGGAAGTTG ACCTTTGCCC TGGCCGCCGC CTTCCAGGAT 
0421 TACTCGCGAC GGGTCAAGGA GGCAACCGGT GAGGAGGAGG GCGAGGCCAC GCCCAGCGAC 
0481 ACTATTACAC CCACGCGACA

Assemble Data

<PRE>

14968R-1.IR_full       1   GATCTGGACAGCCAGGTCAATGTGGAGGATTTGCCCATAACGTTCAAGGTGAAGTACATT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14968R-1.in silico     1   GATCTGGACAGCCAGGTCAATGTGGAGGATTTGCCCATAACGTTCAAGGTGAAGTACATT 60


14968R-1.IR_full       61  GGTTCCGAAGTGGCACGTGGCTTATGGGGCATTAAGTATACGCGTCGTCCGGTGGACATA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14968R-1.in silico     61  GGTTCCGAAGTGGCACGTGGCTTATGGGGCATTAAGTATACGCGTCGTCCGGTGGACATA 120


14968R-1.IR_full       121 ATGGTGGGCGTGGCCAAGAACCTGCCGCCCAATAAGGTGCTGCCCAACTGCGAACTGAAG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14968R-1.in silico     121 ATGGTGGGCGTGGCCAAGAACCTGCCGCCCAATAAGGTGCTGCCCAACTGCGAACTGAAG 180


14968R-1.IR_full       181 GTGTCCACCGACGGAGTCCAGCTGGAGATCATATCGCCAAAGGCCAGCATCAATCACTGG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14968R-1.in silico     181 GTGTCCACCGACGGAGTCCAGCTGGAGATCATATCGCCAAAGGCCAGCATCAATCACTGG 240


14968R-1.IR_full       241 AGCTATCCCATCGACACGATCTCGTATGGCGTTCAGGACCTGGTCTACACAAGGGTCTTT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14968R-1.in silico     241 AGCTATCCCATCGACACGATCTCGTATGGCGTTCAGGACCTGGTCTACACAAGGGTCTTT 300


14968R-1.IR_full       301 GCCATGATCGTGGTGAAGGACGAGTCGAGTCCGCATCCCTTTGAGGTTCACGCCTTCGTG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14968R-1.in silico     301 GCCATGATCGTGGTGAAGGACGAGTCGAGTCCGCATCCCTTTGAGGTTCACGCCTTCGTG 360


14968R-1.IR_full       361 TGCGACAGTCGTGCGATGGCGCGGAA<span class="snp"><tt>A</tt></span>TTGACCTTTGCCCTGGCCGCCGCCTTCCAGGAT 420
                           ||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||
14968R-1.in silico     361 TGCGACAGTCGTGCGATGGCGCGGAA<span class="snp"><tt>G</tt></span>TTGACCTTTGCCCTGGCCGCCGCCTTCCAGGAT 420


14968R-1.IR_full       421 TACTCGCGACGGGTCAAGGAGGCAACCGGTGAGGAGGAGGGCGAGGCCACGCCCAGCGAC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14968R-1.in silico     421 TACTCGCGACGGGTCAAGGAGGCAACCGGTGAGGAGGAGGGCGAGGCCACGCCCAGCGAC 480


14968R-1.IR_full       481 ACTATTACACCCACGCGACA 500
                           ||||||||||||||||||||
14968R-1.in silico     481 ACTATTACACCCACGCGACA 500

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_139543.2	CG14968-RB, transcript variant B (CG14968), mRNA
100	482	0	0	0	NM_168012.1	CG14968-RC, transcript variant C (CG14968), mRNA
100	482	0	0	0	NM_168014.1	CG14968-RE, transcript variant E (CG14968), mRNA
100	482	0	0	0	NM_168013.1	CG14968-RD, transcript variant D (CG14968), mRNA
100	482	0	0	0	NM_168011.1	CG14968-RA, transcript variant A (CG14968), mRNA
0.41	2	2	2	6	NM_132173.2	CG12153-RA (Hira), mRNA
0	0	5	3	2	NM_057664.4	CG15444-RB, transcript variant B (ine), mRNA
0	0	5	3	2	NM_175959.1	CG15444-RD, transcript variant D (ine), mRNA
0	0	4	5	2	NM_079230.2	CG32386-RA (corn), mRNA
0	0	3	2	3	NM_057911.3	CG6382-RA (Elf), mRNA
0	0	2	3	10	NM_167189.2	CG32705-RA (CG32705), mRNA
0	0	2	3	2	NM_057431.2	CG7171-RA (Uro), mRNA
0	0	2	2	3	NM_167193.1	CG32702-RA (CG32702), mRNA
0	0	1	6	5	NM_168898.1	CG32436-RA (CG32436), mRNA
0	0	1	5	6	NM_080327.2	CG3665-RA, transcript variant A (Fas2), mRNA
0	0	1	5	5	NM_167004.1	CG3665-RB, transcript variant B (Fas2), mRNA
0	0	1	5	5	NM_167005.1	CG3665-RC, transcript variant C (Fas2), mRNA
0	0	1	4	7	NM_141262.2	CG12005-RB (Mms19), mRNA
0	0	1	4	6	NM_130550.2	CG11417-RA (CG11417), mRNA
0	0	1	4	3	NM_001043262.1	CG34157-RE, transcript variant E (Dys), mRNA
0	0	1	3	6	NM_135223.2	CG11199-RA, transcript variant A (Liprin-alpha), mRNA
0	0	1	3	6	NM_164707.1	CG11199-RB, transcript variant B (Liprin-alpha), mRNA
0	0	1	3	2	NM_142393.1	CG14321-RA (CG14321), mRNA
0	0	1	2	4	NM_134677.1	CG13690-RA (CG13690), mRNA
0	0	1	2	4	NM_164645.1	CG31915-RA (CG31915), mRNA
0	0	1	2	3	NM_057986.3	CG3456-RA (Mct1), mRNA
0	0	1	2	3	NM_169151.1	CG31286-RC, transcript variant C (CG31286), mRNA
0	0	1	2	3	NM_169152.1	CG31286-RB, transcript variant B (CG31286), mRNA
0	0	1	2	3	NM_141417.2	CG1315-RA (CG1315), mRNA
0	0	0	5	8	NM_080026.2	CG10847-RB, transcript variant B (enc), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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