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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 14895R-2

Symbol Pak3 Full Name Pak3

CG No CG14895 Old CG No CG14895

Synonyms PaK3, DmPAK3, CG14895, D-Pak3, DPAK3, pak3, dPAK3, PAK3, anon-WO0118547.285, Pak3

Accession No (Link to NCBI) NM_142288.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGGAGATGGGGGATCGATCTCA

Primer Seq. 3'

aatctagaggtaccCGGTGCTCCAGATTGGTCTTG

Predicted Fragment Size

IR fragment full Seq

0001 GNAGATGGGG GATCGATCTC AGAGATCGGT GCACCCACAA ACTTCCAACG NCACTTCCAT 
0061 GTCTCGCGCA ACCAGGAAAC CGGCGACCTG GAGGGGCTTC CAGCTCCATG GGTGCGCCTA 
0121 ATGAACTCAC AGATCACTCG CGATGAGCAG GACAAGAATC CAGATGCTGC CTACCATGCC 
0181 GTTAAGTACT ACAACTATTC GATTAAGAAG AAAGAGAACG AGGTCTTCAA GCCCTTCATC 
0241 ACCGAAGATG TGATCCGCGA GGAGTCCAAG GAGATAGAAA ACTATGTCAA CTACAAAAAC 
0301 AAGCACAAGT CTCAGGATCC CGAAAAGTCC GACGACGATG GCAGTTCGAC GGCCACGGAG 
0361 ACGGAAAGTA GCAGCGGTTG TGGCTCCTCG GCAGGTAATA GTAACAGCAG CAGCATCAAC 
0421 GACAGCAGCC AGCAGTCGAC GGTGCCgCTG GACGCCCTAG AGGAGGTGTT CAAGGAGCTC 
0481 AAGACCAATC TGGAGCACCG

in silico PCR Fragment

0001 GGAGATGGGG GATCGATCTC AGAGATCGGT GCACCCACAA ACTTCCAACG GCACTTCCAT 
0061 GTCTCGCGCA ACCAGGAAAC CGGCGACCTG GAGGGGCTTC CAGCTCCATG GGTGCGCCTA 
0121 ATGAACTCAC AGATCACTCG CGATGAGCAG GACAAGAATC CAGATGCTGC CTACCATGCC 
0181 GTTAAGTACT ACAACTATTC GATTAAGAAG AAAGAGAACG AGGTCTTCAA GCCCTTCATC 
0241 ACCGAAGATG TGATCCACGA GGAGTCCAAG GAGATAGAAA ACTATGTCAA CTACAAAAAC 
0301 AAGCACAAGT CTCAGGATCC CGAAAAGTCC GACGACGATG GCAGTTCGAC GGCCACGGAG 
0361 ACGGAAAGTA GCAGCGGTTG TGGCTCCTCG GCAGGTAATA GTAACAGCAG CAGCATCAAC 
0421 GACAGCAGCC AGCAGTCGAC GGTGCCGCTG GACGCCCTAG AGGAGGTGTT CAAGGAGCTC 
0481 AAGACCAATC TGGAGCACCG

Assemble Data


14895R-2.IR_full       1   GNAGATGGGGGATCGATCTCAGAGATCGGTGCACCCACAAACTTCCAACGNCACTTCCAT 60
                           | |||||||||||||||||||||||||||||||||||||||||||||||| |||||||||
14895R-2.in silico     1   GGAGATGGGGGATCGATCTCAGAGATCGGTGCACCCACAAACTTCCAACGGCACTTCCAT 60


14895R-2.IR_full       61  GTCTCGCGCAACCAGGAAACCGGCGACCTGGAGGGGCTTCCAGCTCCATGGGTGCGCCTA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14895R-2.in silico     61  GTCTCGCGCAACCAGGAAACCGGCGACCTGGAGGGGCTTCCAGCTCCATGGGTGCGCCTA 120


14895R-2.IR_full       121 ATGAACTCACAGATCACTCGCGATGAGCAGGACAAGAATCCAGATGCTGCCTACCATGCC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14895R-2.in silico     121 ATGAACTCACAGATCACTCGCGATGAGCAGGACAAGAATCCAGATGCTGCCTACCATGCC 180


14895R-2.IR_full       181 GTTAAGTACTACAACTATTCGATTAAGAAGAAAGAGAACGAGGTCTTCAAGCCCTTCATC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14895R-2.in silico     181 GTTAAGTACTACAACTATTCGATTAAGAAGAAAGAGAACGAGGTCTTCAAGCCCTTCATC 240


14895R-2.IR_full       241 ACCGAAGATGTGATCCGCGAGGAGTCCAAGGAGATAGAAAACTATGTCAACTACAAAAAC 300
                           |||||||||||||||| |||||||||||||||||||||||||||||||||||||||||||
14895R-2.in silico     241 ACCGAAGATGTGATCCACGAGGAGTCCAAGGAGATAGAAAACTATGTCAACTACAAAAAC 300


14895R-2.IR_full       301 AAGCACAAGTCTCAGGATCCCGAAAAGTCCGACGACGATGGCAGTTCGACGGCCACGGAG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14895R-2.in silico     301 AAGCACAAGTCTCAGGATCCCGAAAAGTCCGACGACGATGGCAGTTCGACGGCCACGGAG 360


14895R-2.IR_full       361 ACGGAAAGTAGCAGCGGTTGTGGCTCCTCGGCAGGTAATAGTAACAGCAGCAGCATCAAC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14895R-2.in silico     361 ACGGAAAGTAGCAGCGGTTGTGGCTCCTCGGCAGGTAATAGTAACAGCAGCAGCATCAAC 420


14895R-2.IR_full       421 GACAGCAGCCAGCAGTCGACGGTGCCGCTGGACGCCCTAGAGGAGGTGTTCAAGGAGCTC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14895R-2.in silico     421 GACAGCAGCCAGCAGTCGACGGTGCCGCTGGACGCCCTAGAGGAGGTGTTCAAGGAGCTC 480


14895R-2.IR_full       481 AAGACCAATCTGGAGCACCG 500
                           ||||||||||||||||||||
14895R-2.in silico     481 AAGACCAATCTGGAGCACCG 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	4	NM_142288.2	CG14895-RA, transcript variant A (Pak3), mRNA
47.92	231	1	4	6	NM_169719.1	CG14895-RB, transcript variant B (Pak3), mRNA
0	0	7	11	65	NM_132578.1	CG11146-RA (CG11146), mRNA
0	0	5	27	179	NM_001038734.1	CG16902-RC (Hr4), mRNA
0	0	5	11	55	NM_133033.1	CG12672-RA (CG12672), mRNA
0	0	5	9	90	NM_080105.2	CG31243-RF, transcript variant F (cpo), mRNA
0	0	5	9	55	NM_135615.2	CG6700-RA (CG6700), mRNA
0	0	4	19	45	NM_141216.2	CG14650-RA (CG14650), mRNA
0	0	4	18	85	NM_168237.1	CG17888-RD, transcript variant D (Pdp1), mRNA
0	0	4	12	73	NM_001014517.1	CG2368-RL, transcript variant L (psq), mRNA
0	0	4	12	73	NM_001014520.1	CG2368-RI, transcript variant I (psq), mRNA
0	0	4	12	73	NM_001014518.1	CG2368-RK, transcript variant K (psq), mRNA
0	0	4	12	73	NM_001014519.1	CG2368-RJ, transcript variant J (psq), mRNA
0	0	4	12	72	NM_165792.1	CG2368-RE, transcript variant E (psq), mRNA
0	0	4	12	72	NM_176142.1	CG2368-RG, transcript variant G (psq), mRNA
0	0	4	12	72	NM_176141.1	CG2368-RF, transcript variant F (psq), mRNA
0	0	4	12	72	NM_206086.1	CG2368-RC, transcript variant C (psq), mRNA
0	0	4	12	72	NM_165790.1	CG2368-RA, transcript variant A (psq), mRNA
0	0	4	12	72	NM_165791.1	CG2368-RD, transcript variant D (psq), mRNA
0	0	4	12	72	NM_176143.1	CG2368-RH, transcript variant H (psq), mRNA
0	0	4	12	72	NM_078962.2	CG2368-RB, transcript variant B (psq), mRNA
0	0	4	7	10	NM_136069.2	CG10600-RA (CG10600), mRNA
0	0	4	4	17	NM_133104.2	CG7282-RA (CG7282), mRNA
0	0	4	4	2	NM_164558.1	CG10021-RD, transcript variant D (bowl), mRNA
0	0	4	3	3	NM_133091.2	CG6578-RA (phm), mRNA
0	0	3	45	125	NM_078797.2	CG13109-RA (tai), mRNA
0	0	3	7	23	NM_206067.1	CG1429-RF, transcript variant F (Mef2), mRNA
0	0	3	7	19	NM_057673.2	CG1429-RA, transcript variant A (Mef2), mRNA
0	0	3	7	19	NM_057671.2	CG1429-RB, transcript variant B (Mef2), mRNA
0	0	3	7	19	NM_057672.2	CG1429-RD, transcript variant D (Mef2), mRNA

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