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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 14894R-3

Symbol CG14894 Full Name CG14894

CG No CG14894 Old CG No CG14894

Synonyms CG14894

Accession No (Link to NCBI) NM_142286.2

Inserted Chr. lll

Insertional Mutation lethal

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

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Reference
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Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCAGGCAACGCCAACAGTGACG

Primer Seq. 3'

aatctagaggtaccCCTCACATACTCCGGCCACAG

Predicted Fragment Size

IR fragment full Seq

0001 CAGGCAACGC CAACAGTGAC GACGAATTCC ATGATGCGAT CGGGGAGGAG ATAACCGAAA 
0061 AAGAGGCCAC CAGCACGCGG CAGANCGANA AGGACGTGGA TGAGATCGTG GAGAAGCAAA 
0121 ACCAGCTGGC GTTGGATGAC GAAGCGGAAC AGGGCGCGGC TGGCGGAGAT TCCATCGCGA 
0181 CACCAACGAC AGTGGACTCT GAGCTAACCA TTGAGGAGTT GCGCGAACGG GAGAAGGATC 
0241 TCAGCCCAGA GCAGCTGACA GCTAACAAAG AAAAGGCCGA TAAGCTGAAA GTTGAGGGCA 
0301 ACGAGTTGTT CAAAAATGAC GACGCCGAAN GCGCCGCCAA GACCTACACA GAAGCTTTAG 
0361 ACATTTGCCC CTCGGCCAAC TCCAAGGAGC GAGCAGTTCT CTACGGAAAC AGGGCTGCTG 
0421 CCAAGATTAA GCTGGAGGCC AACAAAGCGG CCATCGACGA CTGCACAAAG GCTATAGAAC 
0481 TGTGGCCGGA GTATGTGAGG

in silico PCR Fragment

0001 CAGGCAACGC CAACAGTGAC GACGAATTCC ATGATGCGAT CGGGGAGGAG ATAACCGAAA 
0061 AAGAGGCCAC CAGCACGCGG CAGAGCGAAA AGGACGTGGA TGAGATCGTG GAGAAGCAAA 
0121 ACCAGCTGGC GTTGGATGAC GAAGCGGAAC AGGGCGCGGC TGGTGGAGAT TCCATCGCGA 
0181 CACCAACGAC AGTGGACTCT GAGCTAACCA TTGAGGAGTT GCGCGAACGG GAGAAGGATC 
0241 TCAGCCCAGA GCAGCTGACA GCTAACAAAG AAAAGGCCGA TAAGCTGAAA GTTGAGGGCA 
0301 ACGAGTTGTT CAAAAATGAC GACGCCGAAG GCGCCGCCAA GACCTACACA GAAGCCTTAG 
0361 ACATTTGCCC CTCGGCCAGC TCCAAGGAGC GAGCAGTTCT CTACGGAAAC AGGGCTGCTG 
0421 CCAAGATTAA GCTGGAGGCC AACAAAGCGG CCATCGACGA CTGCACAAAG GCTATAGAAC 
0481 TGTGGCCGGA GTATGTGAGG

Assemble Data


14894R-3.IR_full       1   CAGGCAACGCCAACAGTGACGACGAATTCCATGATGCGATCGGGGAGGAGATAACCGAAA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14894R-3.in silico     1   CAGGCAACGCCAACAGTGACGACGAATTCCATGATGCGATCGGGGAGGAGATAACCGAAA 60


14894R-3.IR_full       61  AAGAGGCCACCAGCACGCGGCAGANCGANAAGGACGTGGATGAGATCGTGGAGAAGCAAA 120
                           |||||||||||||||||||||||| ||| |||||||||||||||||||||||||||||||
14894R-3.in silico     61  AAGAGGCCACCAGCACGCGGCAGAGCGAAAAGGACGTGGATGAGATCGTGGAGAAGCAAA 120


14894R-3.IR_full       121 ACCAGCTGGCGTTGGATGACGAAGCGGAACAGGGCGCGGCTGGCGGAGATTCCATCGCGA 180
                           ||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||
14894R-3.in silico     121 ACCAGCTGGCGTTGGATGACGAAGCGGAACAGGGCGCGGCTGGTGGAGATTCCATCGCGA 180


14894R-3.IR_full       181 CACCAACGACAGTGGACTCTGAGCTAACCATTGAGGAGTTGCGCGAACGGGAGAAGGATC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14894R-3.in silico     181 CACCAACGACAGTGGACTCTGAGCTAACCATTGAGGAGTTGCGCGAACGGGAGAAGGATC 240


14894R-3.IR_full       241 TCAGCCCAGAGCAGCTGACAGCTAACAAAGAAAAGGCCGATAAGCTGAAAGTTGAGGGCA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14894R-3.in silico     241 TCAGCCCAGAGCAGCTGACAGCTAACAAAGAAAAGGCCGATAAGCTGAAAGTTGAGGGCA 300


14894R-3.IR_full       301 ACGAGTTGTTCAAAAATGACGACGCCGAANGCGCCGCCAAGACCTACACAGAAGCTTTAG 360
                           ||||||||||||||||||||||||||||| ||||||||||||||||||||||||| ||||
14894R-3.in silico     301 ACGAGTTGTTCAAAAATGACGACGCCGAAGGCGCCGCCAAGACCTACACAGAAGCCTTAG 360


14894R-3.IR_full       361 ACATTTGCCCCTCGGCCAACTCCAAGGAGCGAGCAGTTCTCTACGGAAACAGGGCTGCTG 420
                           |||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||
14894R-3.in silico     361 ACATTTGCCCCTCGGCCAGCTCCAAGGAGCGAGCAGTTCTCTACGGAAACAGGGCTGCTG 420


14894R-3.IR_full       421 CCAAGATTAAGCTGGAGGCCAACAAAGCGGCCATCGACGACTGCACAAAGGCTATAGAAC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14894R-3.in silico     421 CCAAGATTAAGCTGGAGGCCAACAAAGCGGCCATCGACGACTGCACAAAGGCTATAGAAC 480


14894R-3.IR_full       481 TGTGGCCGGAGTATGTGAGG 500
                           ||||||||||||||||||||
14894R-3.in silico     481 TGTGGCCGGAGTATGTGAGG 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	1	NM_142286.2	CG14894-RA (CG14894), mRNA
0	0	4	2	4	NM_136413.1	CG12836-RA (CG12836), mRNA
0	0	2	5	2	NM_137567.1	CG9811-RA (Rgk1), mRNA
0	0	2	4	3	NM_143588.2	CG15556-RA (CG15556), mRNA
0	0	1	4	9	NM_136069.2	CG10600-RA (CG10600), mRNA
0	0	1	4	6	NM_134947.1	CG15415-RA (CG15415), mRNA
0	0	1	4	4	NM_169826.1	CG31224-RA (CG31224), mRNA
0	0	1	3	4	NM_139889.1	CG7492-RA (CG7492), mRNA
0	0	1	3	3	NM_057382.2	CG16738-RA (slp1), mRNA
0	0	1	3	2	NM_165112.1	CG31829-RA (CG31829), mRNA
0	0	1	2	13	NM_001043289.1	CG34100-RA, transcript variant A (mld), mRNA
0	0	1	2	3	NM_078659.3	CG5055-RA, transcript variant A (baz), mRNA
0	0	1	2	2	NM_164463.1	CG7082-RD, transcript variant D (CG7082), mRNA
0	0	1	2	2	NM_164462.1	CG7082-RB, transcript variant B (CG7082), mRNA
0	0	1	2	2	NM_134813.2	CG7082-RC, transcript variant C (CG7082), mRNA
0	0	1	2	2	NM_142793.2	CG7054-RA (CG7054), mRNA
0	0	1	2	2	NM_164461.1	CG7082-RA, transcript variant A (CG7082), mRNA
0	0	0	6	5	NM_141779.2	CG6621-RA (CG6621), mRNA
0	0	0	6	5	NM_134724.2	CG4710-RA, transcript variant A (smi21F), mRNA
0	0	0	6	5	NM_164405.1	CG4710-RB, transcript variant B (smi21F), mRNA
0	0	0	6	2	NM_136864.2	CG13185-RA (CG13185), mRNA
0	0	0	5	12	NM_137821.2	CG10955-RA (CG10955), mRNA
0	0	0	5	6	NM_137288.2	CG4439-RA (CG4439), mRNA
0	0	0	5	6	NM_166809.1	CG11154-RB, transcript variant B (ATPsyn-beta), mRNA
0	0	0	5	6	NM_166808.1	CG11154-RA, transcript variant A (ATPsyn-beta), mRNA
0	0	0	5	4	NM_137347.2	CG15605-RA (CG15605), mRNA
0	0	0	5	3	NM_136786.2	CG11979-RA (Rpb5), mRNA
0	0	0	4	15	NM_169056.1	CG31550-RB, transcript variant B (CG31550), mRNA
0	0	0	4	12	NM_167848.1	CG18214-RD, transcript variant D (trio), mRNA
0	0	0	4	11	NM_080325.3	CG6450-RC (lva), mRNA

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