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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 14883R-3

Symbol CG14883 Full Name CG14883

CG No CG14883 Old CG No CG14883

Synonyms CG14883

Accession No (Link to NCBI) NM_142290.1

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

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Reference
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Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTTGTTGTGGCTGGCGCTCAAA

Primer Seq. 3'

aatctagaggtaccTCCAGGAAATCGGACAATTGC

Predicted Fragment Size

IR fragment full Seq

0001 TTGTTGTGGC TGGCGCTCAA AATGATTTAC CAGCTGCTGT gCTGCTCCGT GAGTCTGATC 
0061 TTCTTCTGCT TCAACGTATT CTGGCTCTTT TGCAACCTGG CCATCCCGTG GTGCACTCTT 
0121 ACCTTGCTCG TTGTGTGCAT CNGCCTCCAA GTTCGTCAAA CTGCAACGAA GTCCCAACGA 
0181 GAAGCGACTC CTAAGCCTTC TGAGTTCTCC AGAGGAATGG CCCAGTTACT GGCCAATCGC 
0241 CAACCGGGCA GCCGGCTTTG ATGCGCCCGA AAACTCCAAG GCAGCCATTA AGAANTGCCT 
0301 CGCACGTGGA TATCGGAACG TGCTTCTGGA TGNCgGCCTC ACGTCCTGTG GTGAAATAGT 
0361 CGTGGCTAAC CCCACAAAAA TAAACGTTGc ACAGCCGCTT GTGGAGTTGC AAAAGCTAAA 
0421 CATCACGGAG CAACAcCCGA TGGGGTCGCA GTACGAAGCG GAAACGGTGG CTCCCCTCAG 
0481 GCAATTGTCC GATTTCCTGG A

in silico PCR Fragment

0001 TTGTTGTGGC TGGCGCTCAA AATGATTTAC CAGCTGCTGT GCTGCTCCGT GAGTCTGATC 
0061 TTCTTCTGCT TCAACGTATT CTGGCTCTTT TGCAACCTGG CCATCCCGTG GTGCACTCTT 
0121 ACCTTGCTCG TTGTGTGCAT C-GCCTCCAA GTTCGTCAAA CTGCAACGAA GTCCCAACGA 
0181 GAAGCGACTC CTAAGCCTTC TGAGTTCTCC AGAGGAATGG CCCAGTTACT GGCCAATCGC 
0241 CAACCGGGCA GCCGGCTTTG ATGCGCCCGA AAACTCCAAG GCAGCCATTA AGAAGTGCCT 
0301 CGCACGTGGA TATCGGAACG TGCTTCTGGA TGCCGGCCTC ACGTCCTGTG GTGAAATAGT 
0361 CGTGGCTAAC CCCACAAAAA TAAACGTTGC ACAGCCGCTT GTGGAGTTGC AAAAGCTAAA 
0421 CATCACGGAG CAACACCCGA TGGGGTCGCA GTACGAAGCG GAAACGGTGG CTCCCCTCAG 
0481 GCAATTGTCC GATTTCCTGG A

Assemble Data


14883R-3.IR_full       1   TTGTTGTGGCTGGCGCTCAAAATGATTTACCAGCTGCTGTGCTGCTCCGTGAGTCTGATC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14883R-3.in silico     1   TTGTTGTGGCTGGCGCTCAAAATGATTTACCAGCTGCTGTGCTGCTCCGTGAGTCTGATC 60


14883R-3.IR_full       61  TTCTTCTGCTTCAACGTATTCTGGCTCTTTTGCAACCTGGCCATCCCGTGGTGCACTCTT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14883R-3.in silico     61  TTCTTCTGCTTCAACGTATTCTGGCTCTTTTGCAACCTGGCCATCCCGTGGTGCACTCTT 120


14883R-3.IR_full       121 ACCTTGCTCGTTGTGTGCATCNGCCTCCAAGTTCGTCAAACTGCAACGAAGTCCCAACGA 180
                           ||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||
14883R-3.in silico     121 ACCTTGCTCGTTGTGTGCATC-GCCTCCAAGTTCGTCAAACTGCAACGAAGTCCCAACGA 180


14883R-3.IR_full       181 GAAGCGACTCCTAAGCCTTCTGAGTTCTCCAGAGGAATGGCCCAGTTACTGGCCAATCGC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14883R-3.in silico     181 GAAGCGACTCCTAAGCCTTCTGAGTTCTCCAGAGGAATGGCCCAGTTACTGGCCAATCGC 240


14883R-3.IR_full       241 CAACCGGGCAGCCGGCTTTGATGCGCCCGAAAACTCCAAGGCAGCCATTAAGAANTGCCT 300
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||
14883R-3.in silico     241 CAACCGGGCAGCCGGCTTTGATGCGCCCGAAAACTCCAAGGCAGCCATTAAGAAGTGCCT 300


14883R-3.IR_full       301 CGCACGTGGATATCGGAACGTGCTTCTGGATGNCGGCCTCACGTCCTGTGGTGAAATAGT 360
                           |||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||
14883R-3.in silico     301 CGCACGTGGATATCGGAACGTGCTTCTGGATGCCGGCCTCACGTCCTGTGGTGAAATAGT 360


14883R-3.IR_full       361 CGTGGCTAACCCCACAAAAATAAACGTTGCACAGCCGCTTGTGGAGTTGCAAAAGCTAAA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14883R-3.in silico     361 CGTGGCTAACCCCACAAAAATAAACGTTGCACAGCCGCTTGTGGAGTTGCAAAAGCTAAA 420


14883R-3.IR_full       421 CATCACGGAGCAACACCCGATGGGGTCGCAGTACGAAGCGGAAACGGTGGCTCCCCTCAG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14883R-3.in silico     421 CATCACGGAGCAACACCCGATGGGGTCGCAGTACGAAGCGGAAACGGTGGCTCCCCTCAG 480


14883R-3.IR_full       481 GCAATTGTCCGATTTCCTGGA 501
                           |||||||||||||||||||||
14883R-3.in silico     481 GCAATTGTCCGATTTCCTGGA 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_142290.1	CG14883-RA (CG14883), mRNA
0	0	5	3	4	NM_175947.1	CG33128-RA (CG33128), mRNA
0	0	2	2	5	NM_135887.2	CG3491-RA (CG3491), mRNA
0	0	1	5	3	NM_144370.2	CG17932-RA, transcript variant A (Ugt36Bc), mRNA
0	0	1	5	3	NM_165193.1	CG17932-RB, transcript variant B (Ugt36Bc), mRNA
0	0	1	3	5	NM_135811.2	CG9267-RA (CG9267), mRNA
0	0	1	3	3	NM_134906.1	CG12400-RA (CG12400), mRNA
0	0	1	3	2	NM_139949.2	CG7185-RA (CG7185), mRNA
0	0	1	2	7	NM_206278.1	CG5406-RC, transcript variant C (sif), mRNA
0	0	1	2	7	NM_079908.2	CG5406-RA, transcript variant A (sif), mRNA
0	0	1	2	5	NM_176414.1	CG1988-RB, transcript variant B (CG1988), mRNA
0	0	1	2	4	NM_140493.2	CG16959-RA, transcript variant A (CG16959), mRNA
0	0	1	2	4	NM_168126.1	CG5406-RB, transcript variant B (sif), mRNA
0	0	1	2	4	NM_141430.2	CG1988-RA, transcript variant A (CG1988), mRNA
0	0	1	2	2	NM_136542.1	CG30361-RA, transcript variant A (mXr), mRNA
0	0	0	7	2	NM_135983.1	CG15136-RA (CG15136), mRNA
0	0	0	7	2	NM_165301.1	CG10026-RA, transcript variant A (CG10026), mRNA
0	0	0	7	2	NM_136124.2	CG10026-RB, transcript variant B (CG10026), mRNA
0	0	0	7	2	NM_165302.2	CG10026-RC, transcript variant C (CG10026), mRNA
0	0	0	6	3	NM_135292.1	CG13793-RA (CG13793), mRNA
0	0	0	5	8	NM_001014721.1	CG3620-RC, transcript variant C (norpA), mRNA
0	0	0	5	8	NM_080330.2	CG3620-RA, transcript variant A (norpA), mRNA
0	0	0	5	8	NM_167008.1	CG3620-RB, transcript variant B (norpA), mRNA
0	0	0	5	8	NM_001014720.1	CG3620-RD, transcript variant D (norpA), mRNA
0	0	0	5	3	NM_001043086.1	CG30084-RG, transcript variant G (CG30084), mRNA
0	0	0	5	3	NM_001032250.1	CG30084-RE, transcript variant E (CG30084), mRNA
0	0	0	5	2	NM_137283.2	CG7848-RA (CG7848), mRNA
0	0	0	4	10	NM_137583.3	CG11228-RA (hpo), mRNA
0	0	0	4	3	NM_145757.2	CG30084-RC, transcript variant C (CG30084), mRNA
0	0	0	4	3	NM_001032249.1	CG30084-RF, transcript variant F (CG30084), mRNA

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