NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

14834R-2

Symbol

CG14834

Full Name

CG14834

CG No

CG14834

Old CG No

CG14834

Synonyms

CT34650, CG14834

Accession No (Link to NCBI)

NM_139851.1

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Umetsu D, Dunst S, Dahmann C.<br> An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.<br> PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCGGAAAAGATACGGAAGAGCA

Primer Seq. 3'

aatctagaggtaccATTGGCCGTACGTTGAATGAC

Predicted Fragment Size

IR fragment full Seq

0001 CGGAAAAGAT ACGGAAGAGC AGCCTCAGTG GCTATACGGT GATACTTCTA CTGGCTGCTA 
0061 TTCAGTTCCA AGCAAGTGTA GTGTCCAGCA CGACAACCAA ACCGCCATCG ACATTGGCCA 
0121 CATCCACGAA AGCTAGTCGC AATGTGAATC CCCTGACGGA GTCGAAGCTC GAGAAGAATT 
0181 GCAAGGCGCT GTGTGAGCAT TGCGGCTGTT TGGGCTTCTA CTGCGGCGAG GAGTGCCTGT 
0241 GCGAGTGTAA TGACGACCAA TCCGATACTG AGTGCATTCG CACCATGCAG ATGAATGCCA 
0301 AGACGTTGAA TCTACCATTC GAAATCGTCA TCCAGGGACC GAGCAGCAAT CGGTTTGTGC 
0361 GCAACGCCCA GCAGTTCGAG CAGAAACGAG ATCTGGTGGC CAGGAGCGCC AGCACTTCAA 
0421 TGCAGTCGCC TCGAAATCGT CGCTCAAACA TAACCATCTA CAAGCCCTCC ACAAAGTTGG 
0481 TCATTCAACG TACGGCCAAT

in silico PCR Fragment

0001 CGGAAAAGAT ACGGAAGAGC AGCCTCAGTG GCTATACGGT GATACTTCTA CTGGCTGCTA 
0061 TTCAGTTCCA AGCAAGTGTA GTGTCCAGCA CGACAACCAA ACCGCCATCG ACATTGGCCA 
0121 CATCCACGAA AGCTAGTCGC AATGTGAATC CCCTGACGGA GTCGAAGCTC GAGAAGAATT 
0181 GCAAGGCGCT GTGTGAGCAT TGCGGCTGTT TGGGCTTCTA CTGCGGCGAG GAGTGCCTGT 
0241 GCGAGTGTAA TGACGACCAA TCCGATACTG AGTGCATTCG CACCATGCAG ATGAATGCCA 
0301 AGACGTTGAA TCTACCATTC GAAATCGTCA TCCAGGGACC GAGCAGCAAT CGCTTTGTGC 
0361 GCAACGCACA GCAGTTCGAG CAGAAGCGAG ATCTGGTGGC CAGGAGCGCC AGCACTTCAA 
0421 TGCAGTCGCC TCGAAATCGT CGCTCAAACA TAACCATCTA CAAGCCCTCC ACAAAGTTGG 
0481 TCATTCAACG TACGGCCAAT

Assemble Data

<PRE>

14834R-2.IR_full       1   CGGAAAAGATACGGAAGAGCAGCCTCAGTGGCTATACGGTGATACTTCTACTGGCTGCTA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14834R-2.in silico     1   CGGAAAAGATACGGAAGAGCAGCCTCAGTGGCTATACGGTGATACTTCTACTGGCTGCTA 60


14834R-2.IR_full       61  TTCAGTTCCAAGCAAGTGTAGTGTCCAGCACGACAACCAAACCGCCATCGACATTGGCCA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14834R-2.in silico     61  TTCAGTTCCAAGCAAGTGTAGTGTCCAGCACGACAACCAAACCGCCATCGACATTGGCCA 120


14834R-2.IR_full       121 CATCCACGAAAGCTAGTCGCAATGTGAATCCCCTGACGGAGTCGAAGCTCGAGAAGAATT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14834R-2.in silico     121 CATCCACGAAAGCTAGTCGCAATGTGAATCCCCTGACGGAGTCGAAGCTCGAGAAGAATT 180


14834R-2.IR_full       181 GCAAGGCGCTGTGTGAGCATTGCGGCTGTTTGGGCTTCTACTGCGGCGAGGAGTGCCTGT 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14834R-2.in silico     181 GCAAGGCGCTGTGTGAGCATTGCGGCTGTTTGGGCTTCTACTGCGGCGAGGAGTGCCTGT 240


14834R-2.IR_full       241 GCGAGTGTAATGACGACCAATCCGATACTGAGTGCATTCGCACCATGCAGATGAATGCCA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14834R-2.in silico     241 GCGAGTGTAATGACGACCAATCCGATACTGAGTGCATTCGCACCATGCAGATGAATGCCA 300


14834R-2.IR_full       301 AGACGTTGAATCTACCATTCGAAATCGTCATCCAGGGACCGAGCAGCAATCG<span class="snp"><tt>G</tt></span>TTTGTGC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||
14834R-2.in silico     301 AGACGTTGAATCTACCATTCGAAATCGTCATCCAGGGACCGAGCAGCAATCG<span class="snp"><tt>C</tt></span>TTTGTGC 360


14834R-2.IR_full       361 GCAACGC<span class="snp"><tt>C</tt></span>CAGCAGTTCGAGCAGAA<span class="snp"><tt>A</tt></span>CGAGATCTGGTGGCCAGGAGCGCCAGCACTTCAA 420
                           |||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||
14834R-2.in silico     361 GCAACGC<span class="snp"><tt>A</tt></span>CAGCAGTTCGAGCAGAA<span class="snp"><tt>G</tt></span>CGAGATCTGGTGGCCAGGAGCGCCAGCACTTCAA 420


14834R-2.IR_full       421 TGCAGTCGCCTCGAAATCGTCGCTCAAACATAACCATCTACAAGCCCTCCACAAAGTTGG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14834R-2.in silico     421 TGCAGTCGCCTCGAAATCGTCGCTCAAACATAACCATCTACAAGCCCTCCACAAAGTTGG 480


14834R-2.IR_full       481 TCATTCAACGTACGGCCAAT 500
                           ||||||||||||||||||||
14834R-2.in silico     481 TCATTCAACGTACGGCCAAT 500

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_139851.1	CG14834-RA (CG14834), mRNA
0	0	5	2	6	NM_080026.2	CG10847-RB, transcript variant B (enc), mRNA
0	0	5	2	6	NM_206270.1	CG10847-RA, transcript variant A (enc), mRNA
0	0	4	4	4	NM_140195.2	CG6190-RA (As), mRNA
0	0	4	3	4	NM_205945.1	CG3811-RD, transcript variant D (Oatp30B), mRNA
0	0	4	3	4	NM_135449.2	CG3811-RB, transcript variant B (Oatp30B), mRNA
0	0	4	3	4	NM_164857.1	CG3811-RA, transcript variant A (Oatp30B), mRNA
0	0	4	3	4	NM_205946.1	CG3811-RC, transcript variant C (Oatp30B), mRNA
0	0	3	5	3	NM_080309.2	CG14048-RA (mRpL14), mRNA
0	0	3	4	6	NM_079635.2	CG6384-RA, transcript variant A (Cp190), mRNA
0	0	3	4	6	NM_169632.1	CG6384-RB, transcript variant B (Cp190), mRNA
0	0	2	3	5	NM_167844.1	CG32479-RA (CG32479), mRNA
0	0	2	2	4	NM_132920.1	CG9675-RA (CG9675), mRNA
0	0	2	2	3	NM_168090.1	CG32242-RA (CG32242), mRNA
0	0	1	5	15	NM_079908.2	CG5406-RA, transcript variant A (sif), mRNA
0	0	1	5	15	NM_206278.1	CG5406-RC, transcript variant C (sif), mRNA
0	0	1	4	8	NM_168782.1	CG3961-RB, transcript variant B (CG3961), mRNA
0	0	1	4	8	NM_140810.2	CG3961-RC, transcript variant C (CG3961), mRNA
0	0	1	4	8	NM_168781.1	CG3961-RA, transcript variant A (CG3961), mRNA
0	0	1	4	2	NM_143526.1	CG9737-RA (CG9737), mRNA
0	0	1	3	5	NM_134516.2	CG32529-RA, transcript variant A (CG32529), mRNA
0	0	1	3	4	NM_168126.1	CG5406-RB, transcript variant B (sif), mRNA
0	0	1	3	3	NM_138052.2	CG3356-RA (CG3356), mRNA
0	0	1	3	3	NM_079222.2	CG8339-RA (sfl), mRNA
0	0	1	2	8	NM_132538.1	CG15737-RA (CG15737), mRNA
0	0	1	2	6	NM_134477.1	CG14204-RA (CG14204), mRNA
0	0	1	2	5	NM_170546.1	CG31005-RA (CG31005), mRNA
0	0	0	6	6	NM_057737.2	CG15804-RA, transcript variant A (Dhc62B), mRNA
0	0	0	6	6	NM_206236.1	CG15804-RB, transcript variant B (Dhc62B), mRNA
0	0	0	5	10	NM_080260.2	CG5692-RA (raps), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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