NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

14644R-2

Symbol

CG14644

Full Name

CG14644

CG No

CG14644

Old CG No

CG14644

Synonyms

CG14644

Accession No (Link to NCBI)

NM_141188.4

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCGGAGTACGAGCGAGCGGTAA

Primer Seq. 3'

aatctagaggtaccTTGGCCTTGGCCTCACGCATC

Predicted Fragment Size

IR fragment full Seq

0001 CGNAGTACGA GCGAGCGGTA ATCCTGCGCC TGGGTCgNCT GCGCCCTAAG CCCCCGAggG 
0061 GGCCAGGCGT CATCTTTCTC GTGCCCTGCA TTGATGACCT CGCTGTTGTG GACATACGTA 
0121 CCAGGTCATT TGACCTACAT AGACAGGAAA TCCTCACTCG TGACATGGTG ACGATCAGCA 
0181 TCGACGGAgT GGTGTACTAC AGCATCAAGA GCCCATTCGA CGCCATGCTG CAAGTATACG 
0241 ATCCGNAGNA GGCGACCGAG AAGCTAGCAA TGACCACGCT GCGTAACGTG GCCGGCACCC 
0301 ACAAGCTAAT GGATTTGCTC TCGTCCAAGN AGTACNTCTC CAACCAAATA GAGGGTATAT 
0361 TGTACAACTC CACAGAGCCc tGGGGCATAC GCGTGGAACG GGTCGAGATC AAGGAGATCT 
0421 TCATGCCTGA TCAACTGAAG CGCGCCCTGG CTGTGGAACA GGAgGNGATG CGTGAGgcCA 
0481 AGGCCAA

in silico PCR Fragment

0001 CGGAGTACGA GCGAGCGGTA ATCCTGCGCC TGGGTCGCCT GCGCCCTAAG CCCCCGAGGG 
0061 GGCCAGGCGT CATCTTTCTC GTGCCCTGCA TTGATGACCT CGCTGTTGTG GACATACGTA 
0121 CCAGGTCATT TGACCTACAT AGACAGGAAA TCCTCACTCG TGACATGGTG ACGATCAGCA 
0181 TCGACGGAGT GGTGTACTAC AGCATCAAGA GCCCATTCGA CGCCATGCTG CAAGTATACG 
0241 ATCCGGAGGA GGCGACCGAG AAGCTAGCAA TGACCACGCT GCGTAACGTG GCCGGCACCC 
0301 ACAAGCTAAT GGATTTGCTC TCGTCCAAGG AGTACCTCTC CAACCAAATA GAGGGTATAT 
0361 TGTACAACTC CACAGAGCCC TGGGGCATAC GCGTGGAACG GGTCGAGATC AAGGAGATCT 
0421 TCATGCCTGA TCAACTGAAG CGCGCCCTGG CTGTGGAACA GGAGGCGATG CGTGAGGCCA 
0481 AGGCCAA

Assemble Data

<PRE>

14644R-2.IR_full       1   CG<span class="snp"><tt>N</tt></span>AGTACGAGCGAGCGGTAATCCTGCGCCTGGGTCG<span class="snp"><tt>N</tt></span>CTGCGCCCTAAGCCCCCGAGGG 60
                           ||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||
14644R-2.in silico     1   CG<span class="snp"><tt>G</tt></span>AGTACGAGCGAGCGGTAATCCTGCGCCTGGGTCG<span class="snp"><tt>C</tt></span>CTGCGCCCTAAGCCCCCGAGGG 60


14644R-2.IR_full       61  GGCCAGGCGTCATCTTTCTCGTGCCCTGCATTGATGACCTCGCTGTTGTGGACATACGTA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14644R-2.in silico     61  GGCCAGGCGTCATCTTTCTCGTGCCCTGCATTGATGACCTCGCTGTTGTGGACATACGTA 120


14644R-2.IR_full       121 CCAGGTCATTTGACCTACATAGACAGGAAATCCTCACTCGTGACATGGTGACGATCAGCA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14644R-2.in silico     121 CCAGGTCATTTGACCTACATAGACAGGAAATCCTCACTCGTGACATGGTGACGATCAGCA 180


14644R-2.IR_full       181 TCGACGGAGTGGTGTACTACAGCATCAAGAGCCCATTCGACGCCATGCTGCAAGTATACG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14644R-2.in silico     181 TCGACGGAGTGGTGTACTACAGCATCAAGAGCCCATTCGACGCCATGCTGCAAGTATACG 240


14644R-2.IR_full       241 ATCCG<span class="snp"><tt>N</tt></span>AG<span class="snp"><tt>N</tt></span>AGGCGACCGAGAAGCTAGCAATGACCACGCTGCGTAACGTGGCCGGCACCC 300
                           |||||<span class="snp"><tt>&nbsp;</tt></span>||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||
14644R-2.in silico     241 ATCCG<span class="snp"><tt>G</tt></span>AG<span class="snp"><tt>G</tt></span>AGGCGACCGAGAAGCTAGCAATGACCACGCTGCGTAACGTGGCCGGCACCC 300


14644R-2.IR_full       301 ACAAGCTAATGGATTTGCTCTCGTCCAAG<span class="snp"><tt>N</tt></span>AGTAC<span class="snp"><tt>N</tt></span>TCTCCAACCAAATAGAGGGTATAT 360
                           |||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||
14644R-2.in silico     301 ACAAGCTAATGGATTTGCTCTCGTCCAAG<span class="snp"><tt>G</tt></span>AGTAC<span class="snp"><tt>C</tt></span>TCTCCAACCAAATAGAGGGTATAT 360


14644R-2.IR_full       361 TGTACAACTCCACAGAGCCCTGGGGCATACGCGTGGAACGGGTCGAGATCAAGGAGATCT 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14644R-2.in silico     361 TGTACAACTCCACAGAGCCCTGGGGCATACGCGTGGAACGGGTCGAGATCAAGGAGATCT 420


14644R-2.IR_full       421 TCATGCCTGATCAACTGAAGCGCGCCCTGGCTGTGGAACAGGAGG<span class="snp"><tt>N</tt></span>GATGCGTGAGGCCA 480
                           |||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||
14644R-2.in silico     421 TCATGCCTGATCAACTGAAGCGCGCCCTGGCTGTGGAACAGGAGG<span class="snp"><tt>C</tt></span>GATGCGTGAGGCCA 480


14644R-2.IR_full       481 AGGCCAA 487
                           |||||||
14644R-2.in silico     481 AGGCCAA 487

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	469	0	0	0	NM_141188.4	CG14644-RA (CG14644), mRNA
0	0	4	3	12	NM_206789.1	CG33253-RA (CG33253), mRNA
0	0	4	3	4	NM_164977.1	CG6686-RA, transcript variant A (CG6686), mRNA
0	0	4	3	4	NM_135685.2	CG6686-RB, transcript variant B (CG6686), mRNA
0	0	3	5	5	NM_140191.1	CG6207-RA, transcript variant A (GlcAT-P), mRNA
0	0	3	5	5	NM_001014581.1	CG6207-RC, transcript variant C (GlcAT-P), mRNA
0	0	3	5	5	NM_168451.2	CG6207-RB, transcript variant B (GlcAT-P), mRNA
0	0	2	5	8	NM_140110.2	CG8108-RB, transcript variant B (CG8108), mRNA
0	0	2	5	8	NM_168386.1	CG8108-RA, transcript variant A (CG8108), mRNA
0	0	2	3	4	NM_141502.2	CG10435-RA (CG10435), mRNA
0	0	2	3	3	NM_001043220.1	CG34127-RA (CG34127), mRNA
0	0	2	3	3	NM_169061.1	CG34127-RA (CG34127), mRNA,4,5,-tris-phosphate receptor CG1063-RA, transcript variant A (Itp-r83A), mRNA
0	0	2	3	3	NM_169060.1	CG34127-RA (CG34127), mRNA,4,5,-tris-phosphate receptor CG1063-RA, transcript variant A (Itp-r83A), mRNA,4,5,-tris-phosphate receptor CG1063-RB, transcript variant B (Itp-r83A), mRNA
0	0	2	3	2	NM_135911.1	CG15255-RA (CG15255), mRNA
0	0	1	8	7	NM_136158.2	CG10538-RA (CdGAPr), mRNA
0	0	1	7	4	NM_078977.2	CG10897-RA, transcript variant A (tou), mRNA
0	0	1	4	7	NM_205886.1	CG11838-RB, transcript variant B (Oseg3), mRNA
0	0	1	4	7	NM_134686.1	CG11838-RA, transcript variant A (Oseg3), mRNA
0	0	1	2	6	NM_078592.2	CG11172-RA (NFAT), mRNA
0	0	1	2	3	NM_001043114.1	CG33484-RD, transcript variant D (zormin), mRNA
0	0	1	2	3	NM_001043112.1	CG33484-RB, transcript variant B (zormin), mRNA
0	0	1	2	3	NM_206240.1	CG33484-RA, transcript variant A (zormin), mRNA
0	0	1	2	3	NM_001043113.1	CG33484-RC, transcript variant C (zormin), mRNA
0	0	1	2	2	NM_141918.1	CG10013-RA (CG10013), mRNA
0	0	1	2	2	NM_206650.1	CG2259-RB, transcript variant B (Gclc), mRNA
0	0	1	2	2	NM_080266.2	CG2259-RA, transcript variant A (Gclc), mRNA
0	0	0	8	8	NM_167865.1	CG32333-RA, transcript variant A (CG32333), mRNA
0	0	0	6	6	NM_132430.2	CG11207-RA (feo), mRNA
0	0	0	6	3	NM_169489.1	CG7518-RA, transcript variant A (CG7518), mRNA
0	0	0	6	3	NM_141993.2	CG7518-RB, transcript variant B (CG7518), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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